To search for a safe, effective and non-polluting bio-control microbe of tomato gray mould and tomato leaf mould, 642 endophytic bacterial strains were isolated from healthy tomato plants by tissue-isolation method. Two antagonistic strains of endophytic bacteria Thyy1 and Jcxy8, with strong and steady antagonistic effect on Botrytis cinerea and Fulvia fulva were selected by plat-confront method. Thyy1 and Jcxy8 are preliminarily identified as Bacillus circulans and Bacillus subtilis, respectively, based on physiological and biochemical character measurement. The cultivating medium and conditions of Thyy1 and Jcxy8 strains were then studied. Results indicate that the optimum medium for culturing comprises of a well-ventilated soybean powder with an initial pH of 6.7, duration of 48 h and temperature of 30 ℃.