Leafminer Research Articles

Temporal co-occurrence of leaf herbivory by chewers, leaf miners and gall-formers on a tropical tree: Do leaf traits matter?

Temporal co-occurrence of leaf herbivory by chewers, leaf miners and gall-formers on a tropical tree: Do leaf traits matter?

Estimation of Yield Losses Due to Major Insect Pests of Groundnut

A field experiment was carried to estimate the yield losses due to the groundnut leaf miner Aproaerema modicella (Deventer), tobacco caterpillar Spodoptera litura (F), leafhoppers Empoasca kerri (Pruthi) and thrips Scirtothrips dorsalis (Hood). The results showed the insect pests caused a significant reduction in plant height and other yield attributing characters. They caused a mean reduction of plant height (22%), primary branches (29.1%) reduction in pods/ plant (54.6%) and (44.5) reduction of kernels/ plant. There was a higher yield in the protected plots with 2050 kg/ ha due to spray of insecticides when compared to unprotected plots with 1123 kg/ ha, with a yield increase of 82.5%.

Fertilizer Management, Soil Chemical Properties, Leaf Mineral Contents, and Fruit Quality in Passion Fruit Orchards on Amami-Oshima Island

Fertilizer Management, Soil Chemical Properties, Leaf Mineral Contents, and Fruit Quality in Passion Fruit Orchards on Amami-Oshima Island

Study on the Population Density of Insect Pests of Broccoli, Brassica Oleracea, in the Jadiriyah Area of Baghdad, Iraq

A field study was conducted in the fields of the College of Agricultural Engineering Sciences at the University of Baghdad in Al-Jadriya during the 2018 agricultural season. The objective was to survey the population densities of pests affecting broccoli (Brassica oleracea). The results revealed that the broccoli plants in the open field were affected by various pests. On September 24, the highest density of two-spotted red mite (Tetranychus urticae) eggs reached 35 eggs per leaf. On November 12, the highest density of mite nymphs was recorded at 28 nymphs per leaf. The leaf miner, Liriomyza sativae, exhibited the highest density of 23 tunnels per leaf on September 17. Additionally, the highest density of whitefly nymphs (Bemisia tabaci) was observed on October 1 and November 17, reaching 29 nymphs per leaf. The results also indicated the presence of the aphid lion, Chrysoperla carnea, as a natural enemy. Furthermore, the parasitoid Diglyphus isaea was found on the leaf miner, while the parasitoid Eretmocerus mundus was identified on the whitefly. Therefore, understanding the species of pests and their density attacking agricultural crops is crucial. This knowledge allows for the release of natural enemies in the field to mitigate pest infestations.

The complete mitochondrial genome of Leucoptera coffeella (Lepidoptera: Lyonetiidae) and phylogenetic relationships within the Yponomeutoidea superfamily

The coffee leaf miner (Leucoptera coffeella) is one of the major pests of coffee crops in the neotropical regions, and causes major economic losses. Few molecular data are available to identify this pest and advances in the knowledge of the genome of L. coffeella will contribute to improving pest identification and also clarify taxonomy of this microlepidoptera. L. coffeella DNA was extracted and sequenced using PacBio HiFi technology. Here we report the complete L. coffeella circular mitochondrial genome (16,407 bp) assembled using Aladin software. We found a total of 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs) and an A + T rich-region and a D-loop. The L. coffeella mitochondrial gene organization is highly conserved with similarities to lepidopteran mitochondrial gene rearrangements (trnM-trnI-trnQ). We concatenated the 13 PCG to construct a phylogenetic tree and inferred the relationship between L. coffeella and other lepidopteran species. L. coffeella is found in the Lyonetiidae clade together with L. malifoliella and Lyonetia clerkella, both leaf miners. Interestingly, this clade is assigned in the Yponomeutoidea superfamily together with Gracillariidae, and both superfamilies displayed species with leaf-mining feeding habits.

Olive Performance under the Soil Application of Humic Acid and the Spraying of Titanium and Zinc Nanoparticles under Soil Salinity Stress

Salinity is a major social, economic, and environmental menace in climates with low rainfall and high evapotranspiration, and it influences plant growth and causes restriction to crop production in the world. Additionally, under salinity stress, numerous physiological processes such as photosynthesis, biomass accumulation, and photosynthate transfer are also harshly lessened, and it also limits the absorption of adequate water by plants and leads to a dimension in plant water status. Therefore, the current study was conducted to investigate the soil application of humic acid (HA) at 0, 0.5, 1 and 2 kg/tree alone or in combination with the foliar spraying of 0 mg ZnO2 + 0 mg TiO2, 200 mg ZnO2 + 60 mg TiO2 and/or 300 mg ZnO2 + 80 mg TiO2 through the two successive seasons 2022 and 2023. The results demonstrated that the use of HA alone or in combination with the spraying of TiO2 and ZnO2 greatly improved the leaf chlorophyll, flower number, fruit set percentages, fruit yields in kg or in ton per hectare, fruit weight, fruit size, and fruit firmness. Additionally, the same used treatments greatly improved the fruit content from TSS and oil percentages and also the leaf mineral content from N, P and K, while they minimized the fruit drop percentage and fruit moisture content as compared to control. The most positive influence was observed with the soil implementation of 2 kg HA combined with 300 mg ZnO2 + 80 mg TiO2 in the two experimental seasons.

Revealing a Novel Potential Pest of Plum Trees in the Caucasus: A Species Resembling the European Leaf-Mining Stigmella plagicolella, Nepticulidae.

In instances of severe infestations, Nepticulidae larvae can inflict damage on cultivated plants. Previously, it was assumed that the Prunus-feeding Nepticulidae have continuous distribution from Europe to the neighboring Caucasus. During recent fieldwork in the Caucasus, leaf mines were found on plum trees that initially resembled those of Stigmella plagicolella (Stainton) in Europe. However, upon rearing the adults, significant differences emerged, leading to the hypothesis that a different Prunus-feeding species exists in the Caucasus; this challenges previous records in Western Asia. This paper presents the outcomes of our morphological, molecular, and statistical investigations, unveiling S. colchica sp. nov., a previously unknown potential plum-tree pest. Distinguished by male genitalia characteristics, the new species differs from S. plagicolella. The inter- and intraspecific divergences between S. colchica sp. nov. and S. plagicolella range from 3.5% to 6.02%. Moreover, the utilized delimitation algorithms reliably clustered two species separately, as does our mitotype network. A statistical analysis also shows a discernible trend between the leaf mines of S. colchica sp. nov. and S. plagicolella. This unexpected discovery not only documents a new potential pest, enhancing our understanding of the Caucasian fauna, but also contributes to the broader biological inventory.

Physicochemical characteristics of pomegranate fruits as affected by foliar application of potassium and zinc

Foliar application of mineral nutrients can considerably improve fruit yield and quality. In this study, the effects of K and Zn application by spraying on leaf mineral contents, and some physical and biochemical characteristics of pomegranate fruits cv. ‘Malas Saveh’ were investigated. The mineral treatments were three concentrations of K2SO4 (0, 1, and 2%) and three concentrations of ZnSO4 (0, 0.5, and 1%). The solutions were applied in 2020–2021 and in four times: the first application was during the time of flowering and rest three applications were applied at two-week intervals. The results showed that leaves of trees fed with different concentrations of K2SO4 and ZnSO4 accumulated higher concentrations of N, P K, Zn, Fe, and Mn than those untreated. Pomegranate trees treated with the mineral treatments had higher chlorophyll a and total chlorophyll. Applying most mineral treatments, especially 2% K2SO4+0.5% ZnSO4, significantly increased the fruit yield, fruit diameter, fruit length, aril percentage, aril/skin, the weight of 100 arils, and juice content of 100 g arils, but reduced the cracking percentage, skin thickness, skin percentage and firmness of fruits. Moreover, fruits of pomegranate trees treated with the most mineral treatments and especially 2% K2SO4+0.5% ZnSO4, showed a greater level of TSS (total soluble solids), TSS/TA (total soluble solids/titratable acidity), anthocyanin, total phenol, flavonoid, antioxidant activity, and vitamin C, and a lower level of TA. The study results evidenced that K and Zn application by spraying has a high potential for improving fruit yield and quality of pomegranate.

82 Effects of a red-clover containing mineral on heifer behavior and fertility

Abstract Heat stress reduces reproductive efficiency in beef cattle. Recent evidence suggests that supplementation of red clover can alleviate the physiological impacts of fescue toxicosis which could decrease heat stress. Our objective was to determine the effect of supplementing heifers grazing endophyte-infected tall fescue (E+ TF) with a conventional mineral containing red-clover leaf. We hypothesized that heifers supplemented with the red-clover leaf mineral would have greater conception rates to AI (CRAI) in times of possible heat stress compared with heifers supplemented with conventional mineral. A grazing study was conducted from May – August in 2021, 2022, and 2023. Yearling beef heifers (n = 32, 36, 40 heifers yr-1, respectively) were blocked by body weight, hair coat score and color into 4 grazing blocks that were assigned to 1 of 2 treatments (2 blocks treatment-1): 1. conventional non-medicated loose mineral control (0%-RC), or 2. conventional loose mineral + 20% wt/wt red clover leaf (20%-RC). Mineral treatments were provided in ground, 3-compartment mineral feeders ad libitum. Each grazing block was assigned to four, 0.8- ha. pastures consisting of predominantly E+ TF and were rotationally managed over the course of the study. Each year estrus was synchronized suitable for fixed time AI in May and all heifers resynchronized in June. In addition, each heifer was fitted with an ear-tag sensor (CowManager SensOor, Agis Automatisering BV, Harmelen, the Netherlands) for continuous behavioral monitoring (including high activity and activity). The CRAI were analyzed with the GLIMMIX procedure of SAS using the binomial distribution link logit function (v.9.4., SAS Inst. Inc., Cary, NC). Total daily activity and high activity reported by CowManger during May and June were analyzed by the MIXED procedure with repeated measures of SAS. The CRAI were similar for 20%-RC (67.30 ± 9.80%) and 0%-RC (60.70 ± 9.80%) heifers following the first service of AI (P = 0.51) during a thermoneutral period in May. In contrast, CRAI was greater (P = 0.02) for 20%-RC heifers (48.67 ± 13.47%) than CM (15.10 ± 13.47%) in June when temperatures increased to potential heat stress levels. Overall, conception rate after two rounds of AI for 20%-RC (78.30 ± 9.30%) heifers tended to be greater (P = 0.08) than CM (62.20 ± 9.30 %). While high activity (associated with estrous behavior) reported by CowManager did not differ between treatments (20%-RC: 203.94; 0%-RC: 213.90; SEM: 8.65 min d-1; P = 0.25), normal daily activity of 20%-RC heifers (150.69 ± 10.40 min d-1) tended to be greater (P = 0.06) than CM (126.33 ± 8.65 min d-1). Results of this study suggest supplementing red-clover containing mineral may mitigate negative effects of consuming E+ TF including decreased conception rates during times of heat stress.

Leaf mineral profiling and its correlation with oil physicochemical traits from four olive (Olea europaea L.) cultivars grown in Morocco as affected by olive ripening stages

We investigated genotypic effects on the olive leaves mineral profile and its correlation with soil minerals. Likewise, olive oil extracted from four Mediterranean cultivars (‘Arbequina’, ‘Haouzia’, ‘Menara’, and ‘Picholine Languedoc’) at early and full ripening stages, was studied in terms of basic quality indices, fatty acids, sterols, pigments, and polyphenols. Our outcomes reveal important variations among cultivars and between ripening stages in terms of olive leaf elemental profiling and oil physicochemical traits, while there were no significant (p < 0.05) differences in soil mineral profiling. However, mineral profiling of leaves, basic quality indices, pigments, and polyphenols content of oils showed important inter-cultivar variations. Regarding fatty acid composition, oleic acid (C18:0) was the most abundant. For phytosterols profile, β-sitosterol was found to be the major phytosterol followed by campesterol. Olive oil from fully ripe fruits was marked by reduced chlorophylls (up to − 67.1%), carotenoids (up to − 68.73%) in ‘Menara’ and polyphenols (up to − 45.95%) in ‘Picholine Languedoc’, but an increase of total sterols (up to + 23.5%, ‘Haouzia’). Likewise, saturated (SFA) and monounsaturated fatty acids (MUFA) tended to decrease (up to − 13.5% and − 6.44%, respectively) found in ‘Menara’. However, ‘Arbequina’ had an inceased SFA (+ 7.35%) and MUFA (+ 8.62%). Polyunsaturated fatty acids tended to increase (up to + 41.98%, ‘Menara’) except for ‘Picholine Languedoc’ (− 7.92%). These outcomes were confirmed by principal component analysis with important positive and negative correlations among minerals and oil physicochemical traits. These results showed that the analyzed components could be considered as specific markers to discriminate the studied cultivars.Graphical abstract

Segmentation-based quantification of Tuta absoluta’s damage on tomato plants

The invasion of the tomato leaf miner (Tuta absoluta) poses a significant threat to tomato productivity, leading to substantial yield losses for farmers. Currently, there is a lack of reliable methods for quantifying the effects of Tuta absoluta at an early stage before it causes significant damage. This research proposes a deep Convolutional Neural Network (CNN) model for the segmentation-based quantification of Tuta absoluta on tomato plants. The proposed quantification method employed a Mask RCNN model that achieved a mAP of 85.67 % and precisely detected and segmented the shapes of Tuta absoluta-infected areas on tomato leaves. The ability to accurately detect, segment and count Tuta mines in a tomato leaf image can have a significant impact on the agricultural industry by enabling farmers to quickly assess the extent of damage to their crops and take appropriate measures to prevent further losses.

Use of Images Obtained by Remotely Piloted Aircraft and Random Forest for the Detection of Leaf Miner (Leucoptera coffeella) in Newly Planted Coffee Trees

Brazil is the largest producer and exporter of coffee beans in the world. Given this relevance, it is important to monitor the crop to prevent attacks by pests. This study aimed to detect leaf miner (Leucoptera coffeella) infestation in a newly planted crop based on vegetation indices (VI) derived from aerial images obtained by a multispectral camera embedded in a remotely piloted aircraft (RPA) using random forest (RF). The study was conducted on the Cafua farm in the municipality of Lavras in southern Minas Gerais. The images were collected using a multispectral camera attached to a remotely piloted aircraft (RPA). Collections were carried out on 30 July 2019 (infested crop) and 16 December 2019 (post chemical control). The RF package in R software was used to classify the infested and healthy plants. The t test revealed significant differences in band means between healthy and infested plants, favouring higher means in healthy plants. VI also exhibited significant differences, with EXR being higher in infested plants and GNDVI, GOSAVI, GRRI, MPRI, NDI, NDRE, NDVI and SAVI showing higher averages in healthy plants, indicating distinct spectral responses and light absorption patterns between the two states of the plant. Due to the spectral differences between the classes, it was possible to classify the infested and healthy plants, and the RF algorithm performed very well.

Identification and characterization of lettuce cultivars with high inhibitory activity against the human pathogen Escherichia coli O157:H7: Toward a plant-intrinsic hurdle approach to microbial safety

Foodborne illness linked to fruit and vegetables poses a major challenge to public health and horticulture production. Processed lettuce has been implicated in recurrent outbreaks of pathogenic Shiga toxin-producing Escherichia coli (STEC) infection. We hypothesized that plant defenses elicited by mechanical injury may effect STEC inhibition in cut leaves. Intact and cut leaves of 31 lettuce cultivars (Lactuca spp.) selected from >500 accessions based on their resistance to common insect pests and the necrotrophic lettuce drop pathogen were assessed for STEC serovar O157:H7 (EcO157) survival under cold storage conditions. The cultivars were previously ranked also for phenotypes such as resistance to other phytopathogens and physiological traits. Total leaf phenolic compounds, anthocyanins, and reactive oxygen species (ROS); and phenylalanine lyase (PAL), peroxidase (POD), and polyphenol oxidase (PPO) activity were quantified in each cultivar. Principal Component Analysis (PCA) and K-means cluster analysis were applied to group phenotypes and EcO157 population decline on lettuce. In five cultivars, high resistance to herbivorous insects (aphids, leaf miners and thrips), diseases (lettuce drop, downy mildew, and Impatiens Necrotic Spot Virus), and the disorder tipburn was associated with up to 42-fold EcO157 population decline on cold-stored cut (shredded) leaves, and with up to 11-fold difference in decline on cold-stored intact vs cut leaves (ΔD). The decline on these cold-stored cut cultivars also was up to 24-fold greater than on the cultivar with the lowest decline, and up to 8-fold greater than the mean decline for all 31 cultivars (P < 0.01). Resistance to the hemibiotrophic pathogen causing lettuce bacterial leaf spot, Xanthomonas hortorum pv. vitians, was not indicative of high EcO157 inhibition in this system. High PAL activity, and phenolic and anthocyanin content (PPA); and high PAL and POD activity, were associated with high EcO157 population decline (P < 0.05). One cultivar with high susceptibility to herbivorous insects and phytopathogens, and low levels of defense metabolism nevertheless showed high EcO157 inhibition, suggesting other plant inhibitory characteristics at play. Basal plant immunity and pathways associated with a strong wound response may inform about enteric pathogen survival on minimally processed postharvest lettuce. The related traits identified in this study may serve to breed new genotypes with intrinsically enhanced microbial safety.

Effect of Newer Insecticides against the Major Insect Pests of Groundnut

Efficacy of some newer insecticides were tested against the groundnut leaf miner Aproaerema modicella (Deventer), tobacco caterpillar Spodoptera litura (F), gram pod borer Helicoverpa armigera (Hubner) and natural enemies (coccinellids) of groundnut. Tolfenpyrad @ 2.5ml/ l was significantly superior in controlling defoliator pests. The next best treatments were tolfenpyrad @ 2ml/ l, spinetoram @ 0.5ml/l, tolfenpyrad @ 1.5ml/ l, thiamethoxam+ lambdacyhalothrin @ 0.4ml/ l. The highest incremental cost benefit ratio (ICBR) was obtained in the treatments with tolfenpyrad @ 2.5ml/ l.

Bio-efficacy of insecticide pre mix (Chlorpyriphos 50% + Cypermethrin 5% EC) against ground nut leaf miner in Konkan region

Bio-efficacy of insecticide pre mix (Chlorpyriphos 50% + Cypermethrin 5% EC) against ground nut leaf miner in Konkan region

The explosion of pests and diseases due to climate change

Climate change has been felt over the last few decades in Indonesia, especially in Aceh. Uncertain climatic conditions can affect crop production and food distribution. Insect pests and plant pathogens respond differently to various causes of climate change. An increase in temperature causes changes in the behavior of pests and diseases in terms of their ability to attack. This review discusses the impact of rising temperatures and CO2 levels in the atmosphere on the ability to attack insect pests and pathogens on crops. Global climate warming is expected to trigger an expansion of pests and disease geographic distribution from lowland to highland, increase their survival, and increase the number of new host plants. The survey carried out in Central Aceh on an Arabica coffee plantation showed that leaf spots caused by Hemiliea vastatrix can attack a coffee plantation at an altitude of 1.400 meters, while the same case is also found at the same altitude where coffee berry borer pest (Hyphothenemus hampei) and coffee leaf miner (Leucoptera coffeella) can attack arabica coffee plantation. A comprehensive assessment of the impact of climate change on pests and plant diseases needs to be carried out to get an integrated pest management strategy.

Nutritional properties of Nauclea latifolia root and leaves

Nauclea latifolia is a medicinal plant that is valuable and native to Africa and Asia. It has served as herbal medicine for treating different ailments and diseases. The importance of herbal plants is not just for their medicinal effects but also their nutritional value. This study determined the proximate, vitamin, and mineral compositions of N. latifolia root and leaves using atomic absorption spectroscopy (AAS). The result revealed that N. latifolia root has moisture (5.436%), ash (7.377%), crude fat (5.012%), crude fibre (4.769%), crude protein (6.30%), carbohydrate (71.106%), while the leaf contains moisture (4.127%), ash (5.998 %), crude fat (3.998 %), crude fibre (6.713 %), crude protein (11.90 %), and carbohydrates (67.264%). On the other, vitamins A, C, D and E were 28.65 mg/L, 188.165 mg/100g of extract, 42 mg/L, and 23.86 mg/L, respectively, in the root and 15.197 mg/L, 783.20 mg/100 g, 45.60 mg/L, and 14.095 mg/L, respectively in the leaves. The root and leaf mineral content ranges from 0.351 to 8.278 ppm and 0.135 to 7.988 ppm, respectively. N. latifolia root and leaves have an appreciable content of vitamins A, C, D, and E. They contain reasonable amounts of minerals like phosphorus, calcium, zinc, ıron, magnesium, sodium and potassium. They could be a good source of carbohydrates, protein, and fibre for the development of the body. Their low ash and moisture contents make their storage easy. Therefore, N. latifolia root and leaves will make a good nutrient source for the ever-growing human population

Control of Tuta absoluta (Lepidoptera: Gelechiidae) by the new trend of photosensitizer and nanocomposites and their effects on productivity and storability of tomato

Tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae), is a serious insect pest on tomato plants worldwide. Its larvae can cause up to 100% damage if not controlled. Furthermore, using of chemical pesticides is causing serious threat to environment and human health. The effect of two photosensitizers; magnesium and copper chlorophyllin (Mg-Chl and Cu-Chl) alone and two nanomaterials (GO and Ag) over their photosensitizers (Mg-Chl /Go, Mg-Chl /Ag, Cu-Chl/ Go, and Cu-Chl /Ag) on T. absoluta in tomato field at two seasons were studied. The tested concentrations of photosensitizer and nanocomposites were 10−3 (100 ml/L), 10−4 (10 ml/L) and 10–5 (1 ml/L). The effect of photosensitizer and nanocomposites on reduction % of Tuta absoluta, tomato plants growth, yield, and quality were studied. The number of tunnels was recorded, the reduction percentages for each treatment were calculated compared to control before and after 1, 5, and 7 days of spraying. Results showed that the reduction in the number of tunnels after one day of spraying with photosensitizers (Mg-Chl and Cu-Chl) at the highest concentration (10−3) was 72.79 and 70.52% in the 1st season and 77.95 and 60.08% in the 2nd season. The reduction percentage increased gradually with the number of days after spraying and reached 100% after seven days in both seasons. The reduction percentage reached 100% in plants treated with all nanomaterial concentrations after five days of spraying in the 1st season and after one day of spraying in the second season. Photosensitizer and nanocomposites treatments at all concentrations positively affected all vegetative growth parameters of tomato plants compared with insecticides and control. The highest concentration of all treatments increased yield and enhanced the storability of tomato fruits. The yield after treatment with Mg-Chl was 28.67 tons/fed, which was more than the yield of those treated with Cu-Chl (24.8 tons/fed). Loading nano silver (Ag) over Mg-Chl achieved tomato yield (35.18 tons/fed) compared with the loading of nano graphene oxide (Go) (32.95 tons/fed). Therefore, treatment with these materials can be recommended in the IPM program to control T. absoluta in tomato fields.

First Records of Anthomyiidae (Diptera) from Nagorno-karabakh, Armenia

The root maggot flies are important pests of cultivated and non-cultivated plant species. This study on the invertebrate fauna of Nagorno-Karabakh was focused on the new leaf miners belonging to the Anthomyiidae. Pegomya bicolor (Wiedemann, 1817) and P. betae (Curtis, 1847) are documented for the first time in Nagorno-Karabakh, Armenia; P. bicolor was found on the leaves of Rumex confertus (Willd.), while P. betae infested the leaves of Beta vulgaris L., and Chenopodium album (L.). Thus, two species of the genus Pegomya cause serious damage to fodder and agricultural crops, causing 55 to 90% damage. Details of species description, ecology of larva and pupa, as well as the damage are included.

Genome-Wide Identification of the Genes of the Odorant-Binding Protein Family Reveal Their Role in the Olfactory Response of the Tomato Leaf Miner (Tuta absoluta) to a Repellent Plant

The remarkable biological and evolutionary adaptations of insects to plants are largely attributed to the powerful chemosensory systems of insects. The tomato leaf miner (Tuta absoluta) is a destructive invasive pest with a global distribution that poses a serious threat to the production of nightshade crops, especially tomatoes. Functional plants can attract or repel insect pests by releasing volatiles that interact with the olfactory system of insects, thereby reducing the damage of insect pests to target crops. However, there is limited research on the interaction between T. absoluta olfactory genes and functional plants. In this study, 97 members of the putative odorant-binding protein (OBP) family have been identified in the whole genome of T. absoluta. Phylogenetic analysis involving various Lepidopteran and Dipteran species, including D. melanogaster, revealed that OBP gene families present conserved clustering patterns. Furthermore, the Plus-C subfamily of OBP showed extremely significant expansion. Moreover, the expression levels of the OBP genes varied significantly between different developmental stages; that is, the highest number of OBP genes were expressed in the adult stage, followed by the larval stage, and fewer genes were expressed in high abundance in the egg stage. On the other hand, through a Y-tube olfactometer, we identified a functional plant—Plectranthus tomentosa—that significantly repels adult and larval T. absoluta. Finally, we screened the OBP genes in response to tomato and P. tomentosa volatiles at the genomic level of T. absoluta using RT-qPCR. These results laid a good foundation for controlling T. absoluta with functional plants and further studying olfactory genes.