Methanolic Leaf Extract Research Articles

Antioxidant properties of Carallia brachiata stem bark and leaf

Carallia brachiata (Lour.) Merr. (Rhizophoraceae) had been used to treat various human disoders. This study reported antioxidant activity of C. brachiata of stem bark and leaf in various solvents. Each sample was macerated in various solvent separately. By using DPPH, ABTS, and FRAP assays, antioxidant activity was determined. The TLC method was created using dichloromethane and ethyl acetate mobile phase. The methanol extract of stem bark contained the highest TPC value (262.73 ± 1.58 mg GAE/ g extract), while the methanol extract of leaf contained the highest TFC value (45.46 ± 0.32 mg QE/ g extract). The total phenolic content of stem bark extract was higher than that of leaf extract, but the flavonoid content of the leaf extract was marginally higher. The methanol extract exhibited the highest antioxidant activity for DPPH (13.06±0.08 μg/mL), ABTS (7.19±0.08 μg/mL), and FRAP assay (155.19±0.34 μM Fe2+/g). In this research, stem bark has greater antioxidant activity than the leaf. In addition, the results of TLC profile were detailed. The TLC chromatogram indicates that C. brachiata stem bark contains more components than leaf. This study indicates that C. brachiata has a rich source of prospective antioxidants.

Identification of Phytochemical Compounds Antioxidant Leaf Extract and Clove Flower Extract (Syzygium aromaticum)

One plant known as a flavoring spice, but also as a traditional medicinal plant that is thought to contain antioxidant phytochemical compounds, namely cloves (Syzygium aromaticum) which are widely found in Maluku, Sulawesi and surrounding areas. This study aims to identify the phytochemical compounds of antioxidants of leaf extract and clove flower extract (Syzygium aromaticum) originating from North Sulawesi using leaf methanol extract and clove flowers, where the test was carried out at the Research Center for Spice and Medicine Plant (Ballitro) Bogor included the alkaloid qualitative test , flavonoids, saponins, tannins, and triterpenoids. The results found that there were components of antioxidant phytochemicals in the form of alkaloids, flavonoids, saponins, tannins and triterpenoids in the extraran methanol leaf and clove flowers, with the frequency of existence ranging from there to many.

Exploring the Natural Preservation Potential of Aqueous Guava Leaf Extracts on Pangasius Pangasius: An Experimental Study

Fish, being a significant biological indicator in water, serves as a valuable food source once harvested. However, the high protein content in fish makes it an ideal medium for microorganisms, which can lead to spoilage. In areas without access to freezers or ice, preserving fish becomes a challenge. The objective of this study is to investigate the efficacy of Psidium guajava (guava) leaves as a natural preservation method for Pangasius pangasius fish. Key parameters, including gills, eye, texture, odor, and mucilage, were used to evaluate fish quality. Leaf methanolic extract was applied at doses of 0%, 20%, 40%, 60%, and 80%. Data were collected between 1 and 3 days after storage. Results showed that fish quality declined and began to deteriorate after 2 days of storage, particularly in the control treatment (0% extract). However, the fish samples treated with doses of 60% and 80% experienced relatively good quality over the course of 2 days. Although some spoilage occurred in these samples, they remained suitable for consumption. In contrast, fish samples treated with other doses exhibited complete spoilage and were no longer consumable. In conclusion, guava leaf extracts offer a promising alternative for fish preservation.

Chemical Profiling and Biological Activity of Psydrax dicoccos Gaertn.

Breast cancer is one of the deadliest diseases in women with a mortality rate of 6.6%. Adverse effects of synthetic drugs have directed research toward safer alternatives such as natural compounds. This study focused on Psydrax dicoccos Gaertn, an evergreen tree abundantly distributed in Tamil Nadu (India) for its possible application against breast cancer cells. P. dicoccos leaf methanol extract, found within a wide range of phytochemicals, demonstrated cytotoxic effects against MCF7 breast cancer cells at IC50 of 34 μg/mL. The extract exhibited good antioxidant activities against DPPH• (62%) and ABTS•+ (80%), as well as concentration-dependent (100-800 μg/mL) anti-inflammatory potential of 18-60% compared to standards, ascorbic acid or aspirin, respectively. Moreover, even low extract concentrations (10 μg/mL) inhibited the growth of Escherichia coli (1.9 ± 0.6 mm) and Pseudomonas aeruginosa (2.3 ± 0.7 mm), thus showing high antimicrobial and anti-inflammatory potential. GC-MS and LC-MS analyses identified 31 and 16 components, respectively, of which selected compounds were used to evaluate the interaction between key receptors (AKT-1, COX-2, and HER-2) of breast cancer based on binding energy (ΔG) and inhibition constant (Ki). The results indicate that bioactive compounds from P. dicoccos have potential against breast cancer cells, but further evaluations are needed.

Exploring the anti-cancer potential of methanolic extract from Simarouba glauca: Induction of apoptosis and growth inhibition in lung cancer cells

The causes behind lung cancer etiology remain unknown, and treatment failure is often caused by medication resistance, recurrence, and metastasis. Clinical development of novel therapeutic molecules to overcome drug resistance remains a problem. The purpose of this study was to evaluate the potential of Simarouba glauca triterpenoids among two different plant extracts (leaf extract and bark extract) in two human cell lines: lung adenocarcinoma (A549) and normal lung (L132) cell lines using four different solvents. The concentration yield of methanolic bark extract and leaf extracts was found to be higher compared to the other solvents using the Soxhlet method of extraction. Phytochemical analysis using Liberman-Burchard's test conducted confirmed the presence of triterpenoids in Methanolic extract from barks of Simarouba glauca. Comparative cytotoxicity potential among the extracts from bark and leaves, bark extracts showed an IC50 and LD50 as 350 μg/mL and 300 μg/mL respectively on the A549 cell lines of lung carcinoma. Cell cycle analysis using Methanolic bark extracts in comparison to the leaf extracts showed the arrest of the proliferation of A549 cells at the G0/G1 stage with a significant increase in cells at a level of 42.33%, 23.93%–23.47%, 17.96% respectively. Increased Reactive Oxygen Species (ROS) levels in the A549 cells in 1 h treated with bark extract and leaf extract than in the untreated cells. The methanolic extract from both the bark and leaves was found to be the most potent presenting potential arrest of cell cycle, reactive oxygen species generation and apoptosis. In summary, our study has found that the S. glauca-based plant extract has the anticancer potential by triggering apoptosis.

In-vivo Hepatoprotective Evaluation of Sicyos edulis on Wistar Albino Rats

The liver is known for synthesising enzymes, metabolism, and excretion of drugs and food. However, during biological processes, the abnormality occurs in the liver, which becomes a significant global health burden in humans, characterised by loss of synthetic function, breakdown of blood, irregular vitamin K, and localised, permanent changes to parenchymal cells. The study was designed to research the Phytochemical and biological screening of Sicyos edulis leaf for hepatoprotective activity on laboratory animals using paracetamol and methotrexate model for acute incidence. The study evaluated liver toxicity in healthy Wistar albino rats using two in vivo models. Each study group consists of six animals. In the first model, paracetamol p.o. for seven days. Similarly, in the second model, methotrexate was administered (single dose treatment) to animals with 20mg/kg, b.w., p.o. Both models were challenged with methanolic extract of Sicyos edulis leaf (MESEL) of doses 100mg/kg (low) and 200 mg/kg (high) p.o. for seven days, respectively. On day 8th, the blood samples were collected from the tail vein and analysed for various biochemical parameters. MESEL successfully restored the elevated serum biomarker levels in our study. The decrease in aspartate aminotransferase was observed by removing toxic metabolites, the reduction in alanine aminotransferase was due to an increase in ATP synthesis in mitochondria, thereby modulating the balance of liver energy metabolism, and the decrease in alkaline phosphates is due to tissue regeneration, an increase in total protein denotes the restoration of protein imbalance from acute liver injury. At different concentrations, all these effects strengthen the liver, regulate body metabolism, and ultimately inhibit further liver cell damage in favour of their regeneration. Our study also evidences the protective action of MESEL in rats against the Paracetamol and methotrexate model. The study reveals hepatocyte regeneration followed by hepatic restoration in pre-clinical settings. Keywords: Acute liver disease, Sicyos edulis, Silymarin, Methotrexate, Liver enzymes

Probing the multitargeting potential of n-hexane fraction of Gongronema latifolium leaves in neurodegeneration via in vitro, GC–MS and in silico studies

BackgroundNeurodegenerative disorders (NDDs) are associated with increased activities of brain acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and monoamine oxidase (MAO) as well as Aβ-amyloid (Aβ) neurotoxicity; therefore, they offer a therapeutic option for the treatment of NDDs such as Alzheimer’s disease (AD). This study was aimed at identifying multi-targeting neurotherapeutics from Gongronema latifolium leaves using in vitro analysis, GC–MS profiling and computational methods.ResultsThe n-hexane solvent partition fraction of the methanol extract of Gongronema latifolium leaf (HF) exhibited concentration-dependent inhibitory activities against acetylcholinesterase and butyrylcholinesterase but not against MOA in vitro. The GC–MS chemical profiling identified 17 phytochemicals from the HF; these were further screened against human AChE, BChE, β-secretase enzyme (BACE1) and amyloid-β (Aβ) fibrils using molecular docking, ensemble-based docking (EBD), molecular dynamics simulation (MDs) and binding free energy (BFG) coupled with predictive adsorption, distribution, metabolism, excretion and toxicity (ADMET) analysis. The lead phytochemicals (LPs) (dihydroactinidiolide and 1H-Indole-3-ethanamine), with mean binding energies (− 6.525 ± 0.895 and 6.475 ± 0.985; − 6.833 ± 0.461 and − 6.466 ± 0.577; − 6.2 ± 0.845 and − 5.95 ± 0.353 kcal/mol) exhibited multi-target binding tendencies to the catalytic residues of hAChE, hBChE and hBACE1, in addition to hAβ fibril-disruptive tendencies (− 6.325 ± 0.545 and − 5.95 ± 0.353 kcal/mol), respectively. These results corroborated the initial molecular docking and BFG computations. The lead phytochemical–protein complexes were stable during the period of MDs. The LP presented favorable drug-likeness and ADMET properties coupled with the capacity to traverse the BBB.ConclusionDihydroactinidiolide and 1H-Indole-3-ethanamine, in part or in synergy, are identified as neurotherapeutic constituents of Gongronema latifolium that may have been responsible for the ethnopharmacologically reported neurotherapeutic activities of the leaf, and hence they are suggested as potential drug candidates that can be useful for managing or treating neurodegenerative disease such as Alzheimer's disease, subject to further investigation.

Membrane Stabilization, PhospholipaseA2, Albumin Denaturation, Protease Inhibition, as Viable Mechanisms for the Anti-Inflammatory Effects of Methanol Extract of Rauvolfia vomitoria Afzel Leaves

Background: The body uses inflammation as a defence mechanism to eliminate harmful stimuli like germs, damaged cells, or irritants and to initiate the healing process. However, the ongoing discovery of several medicinal plants and the testing of their bioactivity to produce information that will assist doctors and patients in making informed decisions prior to employing them have established.
 Aims: The study valuate the phosphatelipase A2, membrane stabilization, albumin denaturation, protease inhibition, and platelete aggregation activities as viable mechanisms for the anti-inflammatory effects of the methanol extract of Rauvolfia vomitoria Afzel leaf (MERVL).
 Methodology: The anti-inflammatory effect of (MERVL) was ascertained using the phosphate lipaseA2, membrane stabilization model, albumin denaturation, protease inhibitor, assay.
 Place and Duration of the Study: Department of Pharmacology Lab, Enugu State of University of Science and Technology Agbani Nigeria, between March 2021 and August 2021.
 Results: MERVL inhibited hypotonicity-induced haemolysis by 27.14, 41.10 and 65.70%, at the concentration of 0.4, 0.8 and, 1.0 mg/mL respectively.The highest percentage of inhibition (67.70%) was noticed at the highest concentration of the MERVL. These results were almost analogous to the standard drug (indometacin) used as it exhibited concentration dependent inhibition of albumin denaturation. Protease activity was significantly (P < 0.05) increased at all concentrations which follow the similar tendency as standard drug used. The results showed that MERVL has anti-inflammatory activities.

Long term administration of loquat leaves and their major component, ursolic acid, attenuated endogenous amyloid-β burden and memory impairment

Loquat (Eriobotrya japonica) leaves contain many bioactive components such as ursolic acid (UA) and amygdalin. We investigated the effects of loquat leaf powder and methanol extract in human neuroglioma H4 cells stably expressing the Swedish-type APP695 (APPNL-H4 cells) and C57BL/6 J mice. Surprisingly, the extract greatly enhanced cellular amyloid-beta peptide (Aβ) 42 productions in APPNL-H4 cells. Administration of leaf powder increased Aβ42 levels after 3 months and decreased levels after 12 months compared to control mice. Leaf powder had no effect on working memory after 3 months, but improved working memory after 12 months. Administration of UA decreased Aβ42 and P-tau levels and improved working memory after 12 months, similar to the administration of leave powder for 12 months. Amygdalin enhanced cellular Aβ42 production in APPNL-H4 cells, which was the same as the extract. Three-month administration of amygdalin increased Aβ42 levels slightly but did not significantly increase them, which is similar to the trend observed with the administration of leaf powder for 3 months. UA was likely the main compound contained in loquat leaves responsible for the decrease in intracerebral Aβ42 and P-tau levels. Also, amygdalin might be one of the compounds responsible for the transiently increased intracerebral Aβ42 levels.

Effect of methanolic extract of Ventilago maderaspatana leaves on liver function markers and histopathology of liver tissue in streptozotocin-induced diabetic rats

Aim: The liver is particularly vulnerable to oxidative stress brought on by hyperglycemia since it is the main tissue. In order to better understand how Ventilago maderaspatana leaf methanolic extract (MEVML) affects liver function markers and the histology of liver tissue in STZ-induced diabetic rats, this study looked into the hepatoprotective potential of MEVML. Method: A single STZ dose of 50 mg/kg body weight was given to the animals to cause diabetes. All of the experimental groups' serum was tested for levels of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), γ-glutamyl transferase (GGT), and urea content. Results: The results revealed a marked increase in the serum levels of urea, ALT, AST, ALP, and GGT activity. The results of MEVML treatment, however, were equivalent to those of glibenclamide treatment in that they led to a decline in the activity of ALT, ALP, AST, GGT, and urea content in the diabetic group of rats. Furthermore, diabetic rats' liver tissue showed considerable histological alterations. However, treatment with Ventilago maderaspatana methanolic leaf extract and glibenclamide considerably reduced these histological alterations. Conclusion: Overall, the findings of our study show that MEVML reduces liver function biomarkers and restores the architecture of hepatic tissue to reduce hepatic tissue damage under diabetes stress conditions.

Larvicidal property and active compound profiling of Annona squamosa leaf extracts against two species of diptera, Aedes aegypti and Anopheles stephensi.

Vector control measures are important in lowering the spread of infections spread by mosquito. Synthetic pesticides used to suppress vector populations during the larval stage have had adverse impacts on people and the environment. The early III instar larvae of Aedes aegypti and Anopheles stephensi were the targets of the current experiment, which assessed the larvicidal ability of petroleum ether, chloroform, methanol, and aqueous extracts of Annona squamosa leaves. Using the standard World Health Organization (WHO) larval bioassay test, leaf extracts were evaluated for their activity against Ae. aegypti and An. stephensi to determine lethal doses. Phytochemical analysis and gas chromatography-mass spectrometry (GC-MS) were carried out to identify larvicidal components in the extract. Further analysis using a scanning electron microscope (SEM) was done to check the extracts toxicity for both mosquito larvae. The larvicidal active components were identified by GC-MS as tetradecanoic acid, cis-vaccenic acid, and 2,4-di-tert-butylphenol etc. Methanol leaf extracts of A. squamosa (ASME) exhibited strong larvicidal activity against the early 3rd instar larvae of Ae. aegypti and An. stephensi with Lethal concentration (LC50) values of 51.450 ppm and 107.121 ppm. Cell damages to the larva post exposure to ASME were examined. This finding showed that the ASME has better larvicidal activity and its components that may be used to kill larvae as larvicides. The extracts toxicity towards damage of midgut of larva further suggests that this plant methanol leaf extracts could be effective in larval growth control approaches.

Antibacterial activity of mangrove plant extract of Rhizophora apiculata in inhibiting the growth of various strains of Aeromonas hydrophila

Abstract. Mulia DS, Rahayu SD, Suyadi A, Mujahid I, Isnansetyo A. 2023. Antibacterial activity of mangrove plant extract of Rhizophora apiculata in inhibiting the growth of various strains of Aeromonas hydrophila. Biodiversitas 24: 4803-4810. Aeromoniasis is a bacterial disease caused by Aeromonas hydrophila. Synthetic antibiotics are often used to treat this disease. However, using antibiotics for too long with excessive doses can cause side effects for the environment, fish, and other aquatic biota. One of the natural ingredients with the potential as an antimicrobial is the mangrove plant Rhizophora apiculata. This study aims to determine the antimicrobial activity of mangrove plant extract R. apiculata in inhibiting the growth of various strains of A. hydrophila. The study used a completely randomized design experimental method with a factorial pattern with 3 factors (plant part, bacterial strain, and extract concentration) with 48 treatments and 3 replications. Methanol (polar) and n-hexane (non-polar) were used as extract solvents. The parts of the plant used are leaves and stems. The bacterial strains used were GPl-04, GB-01, GK-01, GJ-01, GL-01, and GL-02. The concentration of mangrove extract used was 0, 10, 20, and 30%. The inhibition zone diameter measured the antimicrobial activity using a paper disc (Kirby Bauer method). The data analysis used was the non-parametric Kruskall-Wallis test and qualitative descriptive. The results showed that R. apiculata methanol extract's leaves and stems contain bioactive compounds of flavonoids, alkaloids, and tannins, while the n-hexane extract contains terpenoids. Leaves methanol extract R. apiculata exhibited better than stems, namely the inhibition zone diameter of 2.87 mm, at a concentration of 20% against the GJ-01 bacterial strain, while the methanol extract of stem R. apiculata showed the best antimicrobial activity, namely the diameter of the inhibition zone of 2.10 mm, at a concentration of 30% against the bacterial strain GL-02. Leaves n-hexane extract R. apiculata had the best antimicrobial activity at a concentration of 10% against the bacterial strain GL-01, with an inhibition zone diameter of 2 mm. The n-hexane extract of stems R. apiculata showed antimicrobial activity at a 30% concentration of 1.23 mm in the same strain. Mangroves plant R. apiculata have bioactive compounds that can be used as natural bactericides to control A. hydrophila bacterial disease.

Screening of Preliminary Phytochemicals and In vitro Antibacterial Activity of Methanolic Extracts of Stem, Leaf, Root and Leaf Callus of Corynandra felina (L.f.) Cochrane & Iltis

Screening of Preliminary Phytochemicals and In vitro Antibacterial Activity of Methanolic Extracts of Stem, Leaf, Root and Leaf Callus of Corynandra felina (L.f.) Cochrane & Iltis

Exploring therapeutic potential of Woodfordia fruticosa (L.) Kurz leaf and bark focusing on antioxidant, antithrombotic, antimicrobial, anti-inflammatory, analgesic, and antidiarrheal properties.

The study aimed to evaluate the pharmacological properties of methanolic extracts of leaves and barks of Woodfordia fruticosa (L.) Kurz (family: Lythraceae) focusing on antioxidant, thrombolytic, anti-inflammatory, antibacterial, analgesic, and antidiarrheal effects. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, clot lysis, disc diffusion, and membrane stabilizing methods were employed to assess in vitro antioxidant, thrombolytic, antibacterial, and anti-inflammatory properties of the leaf and bark methanolic extracts (ME) of W. fruticosa and different organic solvents, that is, petroleum ether (PE), dichloromethane (DCM), chloroform (CL), and aqueous (AQ) fractions. In addition, in vivo central and peripheral analgesic and antidiarrheal activities of both crude extracts were evaluated at two doses (200 and 400 mg/kg of body weight [bw]). All the extracts and fractions showed promising antioxidant properties by scavenging DDPH free radicals with IC50 of 6.11-20.79 μg/mL. AQ fraction (41.24%) of leaves and ME (44.90%) of bark exerted notable in vitro thrombolytic activity. The CL fraction of leaves and AQ fraction of the bark showed 43.16% and 45.37% inhibition of RBC hemolysis, respectively, compared to the inhibition of RBC hemolysis by aspirin in a hypotonic-induced membrane stabilizing assay. Besides, both extracts were observed to provide significant (p < 0.001) central and peripheral analgesic responses at both doses of 200 and 400 mg/kg bw. Furthermore, both doses of bark extract (p < 0.001) and the 400 mg/kg bw of leaf extract (p < 0.05) were observed to possess statistically significant antidiarrheal activity. Additionally, in an in vivo acute toxicity investigation, both extracts had a median lethal dose (LD50) greater than 5000 mg/kg bw, indicating their safety level. The current study proves the ethnomedicinal uses of W. fruticosa; however, further studies are required for phytochemical screening to isolate the responsible bioactive compounds and discover the lead molecules from the plant species.

Estudos histológicos e ultraestruturais sobre o efeito dos extratos metanólicos das folhas de Cassia alata contra o adenocarcinoma pulmonar induzido quimicamente em ratos

ABSTRACT The present work aims to evaluate anticancer performance of Cassia alata methanolic leaf extracts (CMLE) in ethyl carbamate-stimulated lung adenocarcinoma (LAD) in differentiation to the function of Cisplatin (CIPL). Rats were divided into four groups: (1) control (CONT), (2) lung-adenocarcinoma (LAD) injected intra-peritoneally with 1g/kg ethyl carbamate once weekly for a month, (3) LAD+CMLE administered 500 mg/kg CMLE orally for the last two months of the experiment, and (4) LAD+CIPL treated group, injected 2.5 mg/kg Cisplatin intraperitoneally once weekly for the last two months of the experiment. Light and electron microscopic examinations revealed adenocarcinoma development in terminal bronchiole besides some histopathological changes in the LAD group such as atypical, exaggerated collagen fibers, increment of mucinous content, and increasing of PCNA positive immunoreactivity whereas electron microscopy investigation exposed that papillary adenocarcinoma originated from Clara cells in the LAD group. The LAD+CMLE treated group showed no tumor masses and nearly all with normal lung histology. It also recovered the normal ultrastructure of bronchiolar Clara cells. CMLE treatment offers a new alternative cure with less toxicity than Cisplatin for lung cancer therapy. Hence, CMLE would be employed as a novel supply of anti-cancer compounds combating lung cancer.

Cytotoxic Activity of Hypericum triquetrifolium Turra Methanolic Extract Against Cancer Cell Lines.

Since ancient times, several people in the Mediterranean region have employed Hypericum triquetrifolium Turra in traditional medicine. However, only the composition of its essential oils has received extensive research. This study investigated the cytogenetic and cytotoxic effects of H. triquetrifolium methanolic leaf extract on four different cancer cell lines. Methanolic extract of H. triquetrifolium leaves was prepared. Albino male mice were grouped into five: group 1 (blank control) received water;group II (CYP) received cyclophosphoamide; groups III, IV, and V were administered 50, 100, and 200 mg/kg of H. triquetrifolium extracts. On the 11th day, the animals were sacrificed and bone marrowwascollected. The metaphase index (MI) of the bone marrow cells was determined to evaluate the cytogenetic effect of the extract. The cytotoxic activity of the extract was tested on four cancer cell lines (HepG2, PC3, MDA, and A594), while WRL-68 normal cells were employed as control. The index of bone marrow cells in cyclophosphamide (CYP)-treated albino male mice shows a significant difference (P ≤ 0.05) between concentration inhibition 50 % IC50 of cancer cell line compered to WRL-68 normal cells, on MDA and WRL-68 cells (IC50 =185.7, 200.7), HepG2 and WRL-68 (IC50 104.9, 564.6), A549 and WRL-68 (IC50 115, 192), PC3 and WRL-68 (IC50 160.7, 298.7). The results showed that the extracts was able to increase metaphase index compared to cyclophasoamide-treated mice, which caused a drop in the percentage of metaphase index. The current study was showed that significant anti - proliferation activity of Hypericum triquetrifolium methanolic extract against MDA-MB-231 cell line is an epithelial, human breast cancer cell line,WRL-68, HepG2 and lung cancer cell lines (A594).

Phytochemical Studies and GC-MS Analysis of Tinospora Formanii Leaf Extracts

Abstract: The present study deals with the phytochemical aspects of Tinospora formanii. Crude leaf extracts were prepared with different solvents such as hexane, chloroform, methanol, and water using a Soxhlet apparatus. Qualitative and quantitative phytochemical studies and GC-MS analysis of leaf extracts have been carried out. Phytochemical studies showed that methanol leaf extract showed the presence of alkaloids, flavonoids, phenols, sterols, phytosterols, terpenoids, Tannins, saponins, glycosides, carbohydrates Anthraquinones, coumarins, Amino acids. Quantitative estimation showed that the methanol leaf extract exhibited a high flavonoid (74.25±0.885), phenol (57.22±0.135), and alkaloid (49.11±0.356) content. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified compounds such as 4-Hydroxy-3-methoxybenzyl alcohol, Humulen-[V1], Tridecene, Flavone, 9-hexadecenoic acid, Heptadecanoic acid, 1-Tricosene. The recorded phytocompounds are identified as bioactive constituents. The isolation of these bioactive compounds from T. formanii methanol leaf extract and their further studies in vivo animal models may lead to the development of new drugs.

Investigation of Phytochemicals and Antibacterial Activity of Persicaria odorata (L.) Methanolic Leaf Extract Against Selected Bacteria

Multidrug-resistant microorganisms pose serious threats, especially to children from developing countries. Due to this concern, there is a greater need to develop potentially effective antibacterial agents from natural resources. This study aimed to investigate the phytochemical compounds of Persicaria odorata (L.) methanolic leaf extract and its antibacterial activity against selected bacteria. Agar well diffusion and broth microdilution were used to test gram-positive (Staphylococcus aureus and Bacillus cereus) and gram-negative bacteria (Salmonella enterica serovar typhimurium and Shigella flexneri). The bacteria were treated with P. odorata (L.) leaves extracted using methanol and diluted with 10% Dimethyl-sulfoxide (DMSO). P. odorata (L.) leaf extract was highly effective against S. aureus at a concentration of 1,000 mg/mL. Gram-positive bacteria had the lowest MIC and MBC values. The qualitative phytochemical analysis of the methanolic extract of P. odorata (L.) leaves indicated the presence of antibacterial compounds such as phenols, flavonoids, terpenoids, and tannins. In conclusion, P. odorata (L.) leaves which have been widely used in cuisines and traditional medicine also possess the potential to serve as an antibacterial agent.

Computational, chemical profile and in vitro acaricidal property of methanol and chloroform extracts of Cymbopogon citratus Stapf leaf

This study investigated the phytochemical composition, antioxidant and acaricidal properties of methanol and chloroform extracts of Cymbopogon citratus leaf. A known quantity of the pulverized plant material was first exhaustively extracted with chloroform and thereafter, with methanol using a Soxhlet apparatus set at 40 °C. The extracts were concentrated in a hot air oven at 40 °C. Gas chromatography-mass spectroscopy was used to determine the phytochemical composition, while 1,1 diphenyl-2-picrylhydraxyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assay were used to evaluate the antioxidant profile of the extracts at concentrations of 25 - 400 µg/mL. Contact method was used to investigate the acaricidal property and molecular docking analysis of selected identified compounds were performed against acetylcholinesterase to check their drug like potentials. Fifty-seven and sixty-four compounds were identified in the methanol and chloroform extracts, respectively. The methanol extract produced higher antioxidant and acaricidal properties (P &lt; 0.05) relative to the chloroform extract. The compounds formed hydrogen bonds with amino acid residues. In conclusion; the methanol extract demonstrated better antioxidant and acaricidal properties than the chloroform extract, which could be linked to the higher concentration of essential oil (Carvomenthol, (-)-carvone, and eucalyptol) in the methanol extract.

Isolation and Evaluation of Cytotoxic, Anti-Inflammatory, Anti-Ulcer Activity of Methanolic Extract of Ceriops decandra leaves

The current study was undertaken to provide scientific validation for the traditional medicinal applications of Ceriops decandra leaves in treating gastrointestinal disorders and inflammation. Additionally, the study aimed to isolate a pure component from the extracted leaves for further analysis. Lupeol was extracted from the crude methanolic extract of Ceriops decandra leaves by column chromatography as part of a phytochemical inquiry. Its structure was determined using 1H and 13C NMR spectroscopy. In order to assess the cytotoxicity, the unrefined methanolic extract was divided into two fractions: a petroleum fraction and an aqueous fraction, employing the modified Kupchan method. The brine shrimp lethality test revealed that both the aqueous and petroleum ether fractions had significant cytotoxic activity, with LC50 values of 1.93 µg/l and 2.04 µg/l, respectively. These values were compared to the LC50 value of the standard Vincristine Sulphate, which was found to be 0.02 µg/l. The results of the anti-inflammatory trial demonstrated that the administration of the extract at doses of 250mg/kg and 500mg/kg resulted in protection rates of 62.5% and 87.5%, respectively, as compared to the carrageenan control group after 3 hours post-injection. It is worth noting that Ibuprofen exhibited a higher level of protection, with a rate of 91.7%. In the context of ethanol-induced stomach ulcer, the administration of extracts at doses of 250 mg/kg and 500 mg/kg resulted in 45.5% and 59.1% protection against gastric ulcer, respectively. These findings were compared to the protective effect of Omeprazole, which demonstrated 63.6% protection and served as the standard reference. The findings suggest that the methanolic leaf extract of Ceriops decandra possesses robust cytotoxic and potent anti-inflammatory and anti-ulcer properties. These results provide support for the traditional application of this extract in the management of gastrointestinal diseases, inflammation, and cancer.