Methanolic Leaf Extract Research Articles

Biogenic synthesis of silver nanoparticles from the leaf extract of Enicostema axillare (Poir. ex Lam.) A. Raynal and its alpha amylase inhibition activity

Due to its numerous applications, commercial demand for silver nanoparticles produced by biological means is rising. This procedure has no adverse effects, is inexpensive, takes little time, and is non-toxic. The current work uses leaf extracts from Enicostema axillare (Mamajavo) to synthesise silver nanoparticles. The nanoparticles may be characterised by UV–VIS, FT-IR, SEM, TEM, and EDAX. The effectiveness of the screening inhibition assay and alpha-amaylase inhibitory activities is assessed using methanolic leaf extracts and silver nanoparticles. The outcome shows that AgNps exhibit aggregates of grains with a restricted size distribution range between 60 and 80 nm. In comparison to leaf extract, silver nanoparticles made from methanolic leaf extracts have high alpha-amaylase inhibitory action. This study showed that Enicostema axillare's AgNps decrease in the presence of alpha-amylase inhibitors.

Investigation of the Antibacterial Effect of Taraxacum officinale Extracts Extracted in Different Solvents on Bacteroides fragilis ATCC 25285 Standard Strain by Broth Microdilution Method

Bacteria causing hospital-associated infections continue to become resistant due to antibiotic resistance, which has become a global problem worldwide and accordingly, the antibiotic options used in the treatment of infectious diseases caused by these bacteria are limited. In the light of the data obtained from experimental studies on plants, it is thought that plant extracts may be a promising option in the treatment of infectious diseases. In this study, it was aimed to determine the antibacterial activity of Taraxacum officinale extracts on Bacteroides fragilis ATCC 25285 standard strain by broth microdilution method and to pioneer different studies that will investigate the antibacterial effects of plant extracts on resistant B.fragilis strains that cause hospital-acquired opportunistic infections after invasive interventions and trauma. In this study, the T.officinale plant collected as a result of field work was divided into root, leaf and flower parts and dried at 70 °C for 24 hours and then turned into powder. Dried plant samples were extracted in ethanol and methanol for 24 hours. The obtained extracts were stored at -80 °C to be used in the broth microdilution method. B.fragilis ATCC 25285 standard strain was used as the bacterial strain. As a result of the experiments performed with broth microdilution method, the MIC (minimum inhibitory concentration) value of root, leaf and flower extracts with ethanol was determined as 200 μg/ mL, the methanolic root extract as 100 μg/mL and the methanolic leaf and flower extracts as 200 μg/mL. As a result, ethanol and methanol plant extracts were found to be effective on B.fragilis strain.

Phytochemical profiling, antibacterial and antioxidant potential of different parts of invasive plant Sonchus wightianus DC

The aim of the present investigation was to elucidate the phytochemical characterisation, antimicrobial and antioxidant efficacies of methanol extracts of leaf, stem, and root parts of S. wightianus. The phytochemical characterisation by GC-MS analysis revealed the presence of different phytocompounds such as Lupenone (38.02% in leave extract), Leupeyl acetate (33.43% in root extract), 24- Noursa −3, 12- diene (27.16% stem extract). S. wightianus methanol extracts demonstrate IC50, 8.52 µg/ml, 7.72 µg/ml and 9.08 µg/ml using DPPH, FRAP and MCA scavenging assay, respectively. The antibacterial activity of different methanol extracts was evaluated against four microorganisms with a mean zone diameter of inhibition ranging from 13 to 19 mm. For quantitative phytochemical analysis, the highest Total Phenolic Content (TPC) and Total Flavonol Content (TFC-2) were found for steam methanol extract (321.7 mg GAE/g of dry extract, 162.4 mg QE/g of dry extract respectively).

Estimating Antioxidant and Free Radical Scavenging Activity of Arbutus Pavarii Extracts

The primary aim of the current study was to evaluate and compare antioxidant activity in leaves and flowers extracts of arbutus pavarii. For the purpose, the antioxidant potential of crude methanol extracts of arbutus pavarii was screened in vitro using reducing power, phosphor molybdenum assay, and radical scavenging activity by employing DPPH, NO, .OH methods as well as ferric thiocyanate (FTC) and thiobarbituric acid (TBA) tests to confirm the antioxidant potential of these extracts. Resultantly, it was confirmed that the leaves extract (LE) has displayed higher reducing ability compared with flowers extract (FE). The maximum antioxidant activity was found it in leaves methanol extract (199.38±12.73 mg of ascorbic acid/g of dry weight). The Methanolic LE was able to reduce the DPPH concentration with an IC50 of 1.09 ± mg/mL, which was noticeable stronger (P < 0.01) than that of the positive control (ascorbic acid), (IC50 = 0.01 ± 1.6 mg/mL) and FE (IC50= 1.25 ± mg/mL) as well. The LE showed slightly inhibited .OH radical (IC50, 0.78 mg/mL) compared with FE (IC50, 0.91 mg/ml). The obtained results of this investigation indicated the usefulness of utilization of arbutus pavarii leaves as a reliable source of antioxidants for nutritive and industrial purposes.

Phytochemical investigation of Mezoneuron benthamianum Baill, isolation, in vitro antioxidant, alpha-amylase inhibition, and in silico modelling studies

Mezoneuron benthamianum (Leguminosae-caesalpinioideae family), is widely used across West Africa for the treatment of bacterial, fungal, and inflammatory diseases. This study is aimed at investigating the antioxidant and antidiabetic activities of extracts and isolated compounds from Mezoneuron benthamianum leaf and root. The pulverized leaf (1 kg) was extracted by maceration with dichloromethane and methanol to give the dichloromethane (MBLD) and methanol (MBLM) extracts, while the root (2 kg) samples were extracted using dichloromethane and methanol (1:1) to give the dichloromethane-methanol extract (MBRDM) which was further partitioned to give the hexane (MBRH), ethyl acetate (MBRE) and methanol (MBRM) extracts respectively. The leaf methanol extract (MBLM) was fractionated using column chromatography (70–230 mesh size) which led to the isolation of compound 1. The extracts and isolated compounds were subjected to in-vitro antioxidant assay with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and α-amylase inhibitory assay using porcine pancreatic α-amylase respectively. FT-IR spectroscopic analysis compound 1 showed strong peaks at 1678 and 1612 cm−1 suggesting the presence of a carbonyl and an aromatic group respectively. 1H NMR spectroscopic analysis of compound 1 showed two peaks at 3.80 (3H, s) and 7.04 ppm (2H, s) indicating methoxy and aromatic protons respectively. 13C NMR also confirmed the presence of the methoxy peak at 50.8 ppm, four aromatic peaks at 108.76, 120.19, 131.25, 145.14 ppm indicating a para-disubstituted benzene ring, and a carbonyl peak at 167.80 ppm, this led to the elucidation of compound 1 known as methyl gallate. The antioxidant activities of the extracts and compounds showed that MBLM (IC50 = 112.0 μg/ml) and MBRE (IC50 = 91.84 μg/ml) had the highest activity of the leaf and root extracts respectively while methyl gallate (IC50 = 91.84 μg/ml) had lower activity than gallic acid (IC50 = 27.4 μg/ml), ascorbic acid (IC50 = 43.94 μg/ml) and BHT (68.94 μg/ml). The α-amylase inhibitory activities showed that MBLD (IC50 = 27.4 μg/ml), MBRE (IC50 = 72.2 μg/ml), gallic acid (IC50 = 27.4 μg/ml) and methyl gallate (IC50 = 43.9 μg/ml) had higher activity than the standard drug acarbose (IC50 = 378.2 μg/ml). For the In silico study, methyl gallate was docked into the active site of the targeted diabetic proteins; human pancreatic alpha-amylase, pig pancreatic alpha-amylase, and tetrameric IIB-HSDI. The results showed that methyl gallate had good interactions with the proteins, with binding affinities of −4.8, −4.8 and −6.5 kcal/mol respectively. Furthermore, in silico pharmacokinetics and toxicology properties of methyl gallate using the AdmetSAR and SwissADME software showed that the compound has good pharmacokinetic properties and it is generally non-toixc. This research has revealed detailed chemical and biological studies on Mezoneuron benthamianum and its isolated compound as an antioxidant and antidiabetic.

Chemical Composition of Methanol Extracts from Leaves and Flowers of Anemonopsis macrophylla (Ranunculaceae).

Anemonopsis Siebold et Zucc. is an unstudied single-species genus belonging to the tribe Cimicifugeae (Ranunculaceae). The only species of this genus-Anemonopsis macrophylla Siebold and Zucc.-is endemic to Japan. There are no data on its chemical composition. This work is the first to determine (with liquid chromatography-high-resolution mass spectrometry, LC-HRMS) the chemical composition of methanol extracts of leaves and flowers of A. macrophylla. More than 100 compounds were identified. In this plant, the classes of substances are coumarins (13 compounds), furocoumarins (3), furochromones (2), phenolic acids (21), flavonoids (27), and fatty acids and their derivatives (15 compounds). Isoferulic acid (detected in extracts from this plant) brings this species closer to plants of the genus Cimicifuga, one of the few genera containing this acid and ferulic acid at the same time. Isoferulic acid is regarded as a reference component of a quality indicator of Cimicifuga raw materials. The determined profiles of substances are identical between the leaf and flower methanol extracts. Differences in levels of some identified substances were revealed between the leaf and flower extracts of A. macrophylla; these differences may have a substantial impact on the manifestation of the biological and pharmacological effects of the extracts in question.

Hypoglycemic, antidiabetic and toxicological evaluation of leaf extract of Xylopia parviflora (A. rich.) Benth

BackgroundAlmost all Xylopia species have a long history of being used therapeutically in China due to their potent antioxidant properties. Xylopia parviflora (X. parviflora) is a relatively novel plant comprised mainly of beta-pinene, which has a rich history in Chinese medicine and is a principal active constituent in numerous Chinese herbs such as Rakkyo, the Chinese onions, Huasi Baidu Fang and Ru Xiang which are all native to China. ObjectiveThe study evaluated the antidiabetic potentials of the methanol leaf extract of X. parviflora and its toxicity profile. MethodsA modified version of the OECD guidelines was used to conduct acute and sub-chronic toxicity tests on the plant extract. The extract's effects on glucose, haematological, and biochemical components were evaluated using standard procedures. Using glibenclamide (10 mg/kg) and distilled water as positive and negative controls, the extract was tested for its antidiabetic activity in diabetic rats with streptozotocin induction at 50, 150, and 300 mg/kg for 28 days. Furthermore, vital organs underwent histopathological examination. Analysis of Variance (ANOVA) and the Dunnett multiple comparison tests were used for the statistical analysis, with a significance level of p < 0.05 ResultsPreliminary phytochemical analysis of the leaf extract revealed the presence of tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars, and anthraquinones. The extract's median lethal dose (LD50) was greater than 2000 mg/kg in mice. Although it significantly reduced blood glucose levels in normal rats at 150 and 300 mg/kg doses, it had no toxic effects on the haematological and biochemical components of the collected blood samples. The extract produced an antidiabetic, non-dose-dependent impact on day seven at all the tested doses in streptozotocin-induced rats. On days 14, 21, and 28, however, glibenclamide and extract activity at all tested doses were comparable. At 200 mg/kg, the extract did not affect the histology of the liver, brain, kidney, or pancreas, but at 400 and 800 mg/kg, it had a slight and severe impact on these organs, respectively. ConclusionAccording to the study's findings, X. parviflora has antihyperglycemic activity and is non-toxic at low doses but may be harmful at high doses.

Cercaricidal efficacy of plant extract: evidence from the methanolic leaves and bark extracts of &lt;i&gt;Anacardium ocidentale&lt;/i&gt; (Linnaeus)

Schistosomiasis continues to be a public health problem causing in unsatisfactorily high level of morbidity. Praziquantel is a recent medicine for treatment but unable to kill emerging schistosomes. It does not prevent re-infection and its constant application may lead to the advent of drug-resistant parasites. Qualitative phytochemical screening of the methanol crude extract of A. occidentale revealed the presence of Tannins, Flavonoids and Saponins. Cercaricidal activity on Gymnocephalous cercaria, after 120 minutes of contact in a concentration of 0.10 mg/mL, showed that there was 92 and 34% death when exposed to the methanol leave and bark extracts of A. occidentale leaves, and A. occidentale bark respectively. There was 99% and 98.5% mortality between the methanol bark extract of A. occidentale and the leaf of A. occidentale plant respectively in a concentration of 0.60mg/mL. Lethal concentration on Gymnocephalous cercariae showed that A. occidentale methanolic bark extract had the highest LC50 and LC84 of 0.151 and 0.204 mg/mL respectively while A. occidentale methanol leaf extract had the lowest LC50 and LC84 of 0.123 and 0.162 respectively. A. occidentale methanol bark extract had the lowest LC50 of 0.237 and A. occidentale methanol leaf extract had the lowest LC 84 of 0.316 mg/ml. The correlation coefficient of the plant parts showed a strong positive correlation between the log concentrations and probit mortality. The extracts had an effect on the cercariae tested. A. occidentale leaves extract showed highest cercaricidal activity. Gymnocephalous cercariae was more susceptible to the plant parts than the Brevifurcate cercaria

A potential antiviral against COVID-19 obtained from Byrsonima coccolobifolia leaves extract

In this study, we specifically focused on the crude methanolic leaf extract of Byrsonima coccolobifolia, investigating its antifungal potential against human pathogenic fungi and its antiviral activity against COVID-19. Through the use of high-performance liquid chromatography coupled with electrospray ionization ion trap tandem mass spectrometry, direct infusion electrospray ionization ion trap tandem mass spectrometry, and chromatographic dereplication procedures, we identified galloyl quinic acid derivatives, catechin derivatives, proanthocyanidins, and flavonoid glycosides. The broth dilution assay revealed that the methanolic leaf extract of B. coccolobifolia exhibits antifungal activity against Cryptococcus neoformans (IC50 = 4 μg/mL). Additionally, docking studies were conducted to elucidate the interactions between the identified compounds and the central residues at the binding site of biological targets associated with COVID-19. Furthermore, the extract demonstrated an in vitro half-maximum effective concentration (EC50 = 7 μg/mL) and exhibited significant selectivity (>90%) toward SARS-CoV-2.

Antioxidant and acetylcholinesterase inhibitory activities, in silico analyses, and anti-Alzheimer’s disease potential of leaf extracts of three Nigerian endemic medicinal plants (Spondias mombin, Carica papaya and Kalanchoe crenata)

BackgroundThe evaluation of the correlations between antioxidant and anti-acetylcholinesterase activities of methanol leaf extracts of three Nigerian endemic plants, Spondias mombin, Carica papaya and Kalanchoe crenata, was carried out. Their constituent phytochemicals were identified by HPLC–DAD fingerprinting. The antioxidant activity as typified by 2,2-diphenyl-1-picrylhydrazyl (DPPH·), 2,2′-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid (ABTS·+) and nitric oxide (NO) scavenging activities were evaluated. The acetylcholinesterase (AChE) inhibitory activity of the extracts was also determined.ResultsThe extracts contained appreciable amounts of the flavonoids, quercetin and kaempferol. The extracts of Spondias mombin, Carica papaya and Kalanchoe crenata showed concentration-dependent inhibitory activities against DPPH· and ABTS·+ with IC50 of 43.29 ± 0.443 µg/mL, 59.27 ± 0.644 µg/mL and 80.20 ± 0.414 µg/mL; 25.43 ± 0.325 (µg/mL), 39.84 ± 0.163 µg/mL and 59.02 ± 0.376 (µg/mL), respectively. The IC50 for the NO scavenging activities of the Spondias mombin, Carica papaya and Kalanchoe crenata extracts were 41.99 ± 0.217 µg/mL, 50.44 ± 0.281 µg/mL and 60.12 ± 0.512 µg/mL, respectively. The IC50 for the inhibitory effects on AChE was 53.24 ± 0.327 µg/mL, 60.95 ± 0.290 µg/m and 70.5 ± 0.426 µg/mL, respectively. The effectiveness of the plant in all the experimental tests was in the following order: S. mombin > C. papaya > K. crenata. The total flavonoid and total phenolic contents have extremely significant positive correlations with the antioxidant activities and AChE inhibitory activity. The correlation coefficients (r2) of DPPH scavenging activity and NO scavenging activity with the AChE inhibitory activity were 0.8295 µg/mL and 0.7337 µg/mL, respectively (P < 0.0001). The molecular docking and pharmacokinetic analyses on some constituent phytochemicals showed that quercetin, kaempferol, ferulic acid, leucocyanidin, gallic acid and isorhamnetin fulfilled the requirements for an anti-Alzheimer drug.ConclusionsThe results suggest that the plant species provide a significant source of secondary metabolites that can act as natural antioxidants and acetylcholinesterase inhibitors, which will be helpful in the treatment of Alzheimer’s disease.

Phytochemical Investigation and Toxicological Insights of Cassia sieberiana Leaf Extract: Implications for Medicinal use

Introduction: Cassia sieberiana, a member of the Fabaceae family, has a rich history of traditional medicinal uses. This study focuses on exploring the medicinal potential of the methanol extract from Cassia sieberiana leaves. The research aims to conduct a comprehensive analysis of bioactive compounds and assess acute toxicity through LD50 determination.&#x0D; Methods: Fresh leaves were collected from Opi town, Nsukka, Nigeria, and authenticated. Male Wister albino rats were acclimatized, and phytochemical screening was performed using qualitative and quantitative methods. LD50 determination followed internationally recognized protocols, employing mice models.&#x0D; Results: The methanol extract exhibited a yield of 11.2%. Phytochemical analysis revealed the presence of proteins, carbohydrates, tannins, alkaloids, steroids, cardiac glycosides, saponins, flavonoids, reducing sugars, terpenoids, and quinones. Alkaloids were predominant (1770.8±74.43 mg/100g). LD50 determination showed 100% survival at 5000 mg/kg, indicating relative safety.&#x0D; Discussion: The high yield suggests methanol as an effective solvent. Phytochemical composition aligns with traditional uses, and the prevalence of alkaloids supports reported medicinal applications. Low phenolic content suggests antioxidant effects, while flavonoids may contribute to anti-inflammatory properties. Saponins and tannins indicate potential for antimicrobial and purgative use. The study affirms the safety of the methanol leaf extract.&#x0D; Conclusion: The study provides valuable insights into the medicinal potential of Cassia sieberiana. The significant alkaloid content, diverse bioactive compounds and demonstrated safety in LD50 determination support its traditional uses. These findings lay a robust foundation for further exploration of Cassia sieberiana in drug development and healthcare applications.

Effects of methanolic leaf extract and fractions of Irvingia gabonensis on hematological parameters in Wistar rats with splenomegaly

Splenomegaly is a medical condition characterized by an enlargement of the spleen, an organ located in the upper left part of the abdomen, beneath the ribcage. The spleen plays various essential roles in the body, including filtering blood, removing old or damaged blood cells, and helping the immune system by producing and storing immune cells. Splenomegaly causes negative haematological effects. It is very common in Africa and Asia. The aim of this study is to investigate the effect of methanol leaf extract and fractions of Irvingia gabonensis on haematological parameters in splenomegaly-induced Wistar rats. Extraction and fractionation of the plant were carried out through standard procedure using methanol, hexane, butanol and ethyl acetate. A qualitative phytochemical evaluation of the plant was done. The effects of the extract and fractions of Irvingia gabonensis were investigated using a rat model. The presence of various phytochemicals like alkaloids, flavonoids, steroids, terpenoids, and tannins was observed in the extract and fractions of the plant. The methanolic extract and ethylacetate fraction significantly caused the normalization of PCV, HB, and RBC post-treatment. The findings from this study suggest that Irvingia gabonensis could be researched further for the management of splenomegaly and also the isolation of phytochemicals responsible for these effects.

Phytochemical Profiling, Green Synthesis, and Bioactivity Evaluation of Silver Nanoparticles (AgNPs) Synthesized from Ipomoea laxiflora Extract

This study focuses on the eco-friendly production of silver nanoparticles using leaf extract from Ipomoea laxiflora and assesses their antioxidant and hemolytic effects. To our knowledge, this is the first report on the synthesis of silver nanoparticles using this species. Green synthesis offers immense potential in both medical and environmental fields, aiming to utilize less hazardous chemicals. Plant-based synthesis, in particular, is considered safe and effective due to the presence of reducing and capping agents within plant extracts. Ipomoea laxiflora H.J. Chowdhery &amp; Debta, belonging to the Convolvulaceae family, is an annual climber native to Tropical Africa and India. It has been traditionally used to treat fever, headaches, and stomach aches. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, phenols, tannins, terpenoids, steroids, glycosides, and cardio glycosides. Quantification of phytochemical contents, including total phenolic, flavonoid, and proanthocyanin content, was also performed. FT-IR spectroscopic analysis indicated characteristic peak values of major functional groups such as alkene, alkane, and carbonyl. Silver nanoparticles were synthesized by adding 10 mL of methanolic leaf extract to 90 mL of 1 mM aqueous silver nitrate solution, followed by heating at 80 degrees Celsius for three hours with continuous stirring. The change in color from yellow to dark brown confirmed the formation of silver nanoparticles. The antioxidant activity, determined by DPPH radical scavenging assay, showed 94% scavenging activity for methanolic extract compared to 98% for ascorbic acid. Higher concentrations exhibited increased scavenging activity. Total antioxidant activity ranged from 60% to 89% in hexane and methanol extracts, with methanol showing the highest concentration. Hemolytic activity was observed between concentrations of 10 to 100 µg/ml, with a hemolysis rate of 2.751% at a concentration of 100 µg/mL. The development of green nanoparticles using natural sources like Ipomoea laxiflora holds significant importance for environmental sustainability, health benefits, diverse biomedical applications, resource efficiency, and cost-effectiveness. Embracing such green approaches not only advances nanotechnology but also aligns with broader goals of promoting sustainable development.

Isoflavone-Rich Extract of Trifolium resupinatum: Anti-obesity Attributes with In Silico Investigation of Its Constituents

Trifolium resupinatum L., Fabaceae, aqueous methanol leaf extract was selected to mitigate some obesity-associated risk factors to validate the possibility of further developing herbal drugs. Chromatography and spectrophotometric techniques verified 14 phenolics, five of which were first isolated from the plant and identified as 6''-O-acetyl ononin, genistin, daidzin, sissotrin, and astragalin. Further phytochemical characterization was performed via liquid chromatography-electrospray ionization-mass spectrometry assisted by a spectral similarity molecular network. In total, 81 metabolites were tentatively annotated including 69 species-first dereplications. Two major isolates (formononetin and pseudobaptigenin) were selected along with the investigated extract for an in vitro pancreatic lipase inhibition assay. They showed notable effects with IC50 values (µg/ml): 47.2 ± 1.1, 112.8 ± 1.23, and 471.32 ± 0.8, respectively, incomparable to orlistat (23.8 ± 0.64). Preliminary in vivo assay (25 mg/kg extract, daily, 8 weeks) displayed weight loss interest and promising advancement of serum triacylglycerides, total cholesterol, and glucose levels. Molecular docking studies confirmed the promising binding score of formononetin and pseudobaptigenin near the active sites and highlighted the affinity of other isolates to the lipase enzyme. Several isolates passed Lipinski’s law of the drug-likeness test, whereas SwissADME radar displayed that all constituents fall within the acceptable bioavailability zone. Therefore, the combination of flavonoids, especially isoflavones, could be regarded as drug-like agents for protection against obesity-induced metabolic complaints.Graphical

Evaluation of the antidiarrheal activity of the Heliotropium indicum (Indian heliotrope) methanol leaf extract and formulation into oral suspensions

ABSTRACT Introduction: For many years, diarrhoea has been a leading cause of millions of annual deaths in both developing and developed regions. Consequently, searching for novel sources of antidiarrheal drugs has become a key emphasis in contemporary research Aim: This study aims to evaluate the safety and antidiarrheal potential of Heliotropium indicum methanol leaf extract (HIMLE) and to formulate stable suspensions for further application. Methods: The safety and antidiarrheal potential of Heliotropium indicum methanol leaf extract (HIMLE) were assessed, and stable suspensions were formulated and characterized. Phytochemical constituents were determined using standard methods Acute toxicity testing, conducted under the Organization for Economic Cooperation and Development-423 guidelines, revealed a lethal dose (LD50) greater than 3000 mg/kg body weight (bw). Antidiarrheal effects were evaluated using a castor oil-induced diarrhoea model in Wistar rats, with groups receiving different doses of HIMLE and control. Results: The phytochemical screening revealed the presence of Flavonoids, Phenols, Tannins, Saponins, Alkaloids and Steroids. The extract demonstrated a dose-dependent reduction in intestinal transit and frequency of watery stool, with promising results compared to the positive control, loperamide. Structured vehicle suspensions, formulated at 1.25% w/v with carboxyl methylcellulose, polyvinylpyrrolidone, and tragacanth, exhibited stability over four weeks. The suspensions effectively inhibited diarrhoea in a dose-dependent manner. Formulations 4.60±2.73. 4.64±1.64 and 4.73±1.91 exhibit similar pH trends with slight variations, while Formulations 6.50±0.23 and 6.70±0.23 demonstrate higher pH values; additionally, viscosity tends to decrease as rotational speed decreases across all formulations. at 60 rpm.. Conclusion: The study suggests that HIMLE possesses significant antidiarrheal activity. The formulated suspensions proved effective in controlling diarrhoea in Wistar rats, highlighting the potential therapeutic application of Heliotropium indicum methanol leaf extract. His significant antidiarrheal activity could be accounted for by pharmacological effects. Keywords: Heliotropium indicum, methanol leaf extract, acute toxicity, structured vehicle, antidiarrhea

Toxicological Evaluation of Crude Musa sapientum L (Banana) Methanol leaves Extract of in wistar albino rats

Abstract Introduction: Musa sapientum, L commonly known as banana, has been traditionally used for both nutritional and medicinal purposes across various cultures. Different parts of the plant are believed to possess therapeutic properties. Aim: This study aims to assess the toxicological profile of Musa sapientum L. methanol leaf extract (MSMLE) in Wistar albino rats to evaluate its safety and potential medicinal benefits. It focuses on the phytochemical constituents, determination of the lethal dose 50 (LD50), and subchronic effects on liver and kidney biomarkers, as well as hematological parameters. Materials and Methods: Phytochemical constituents of MSMLE were determined using standard methods. The LD50 was determined following OECD guidelines by administering fixed doses to groups of rats. For the subchronic study, rats were divided into five groups: a control group receiving distilled water (2 mg/kg) and four groups receiving escalating doses of MSMLE (150, 300, 600, and 1200 mg/kg) orally for 28 days. Graph Pad Instat Version 3.0 was used for data analysis. Results: Phytochemical screening confirmed the presence of alkaloids, steroids, flavonoids, tannins, and saponins in MSMLE. The LD50 was found to be greater than 3000 mg/kg, indicating low acute toxicity. Significant decreases (P&lt;0.05) in alkaline phosphatase (ALP), albumin (ALB), and alanine transaminase (ALT) were observed only in the group receiving the highest dose (1200 mg/kg) compared to the control. Kidney function biomarkers also showed significant decreases (P&lt;0.05) in the high-dose group. Hematological parameters, including red blood cells (RBC), white blood cells (WBC), hemoglobin (HGB), and neutrophils, decreased significantly (P&lt;0.05), while mean corpuscular volume (MCV) and lymphocytes showed a significant increase. Conclusion: The study concludes that MSMLE demonstrates low acute toxicity with an LD50 above 3000 mg/kg. However, subchronic administration at the highest dose (1200 mg/kg) resulted in significant changes in liver and kidney biomarkers, as well as hematological parameters, suggesting potential adverse effects at high doses. Further research is recommended to establish safe dosages and explore long-term effects before recommending extended use of MSMLE for therapeutic purposes Keywords: Musa sapientum Lin, Toxicity evaluation, Phytochemical analysis, traditional medicine, and Wistar albino rats

Effect of Plumbago zeylanica L. (Ceylon leadwort) methanol leaf extract on the hepatic profile of male Wistar rats

Effect of Plumbago zeylanica L. (Ceylon leadwort) methanol leaf extract on the hepatic profile of male Wistar rats

Evaluation of Hepatoprotective Potential and oxidative stress of Allium ascalonicum L. methanol leaf extract on liver of Albino rats

Background: For decades, the leaves of Allium ascalonicum (commonly known as Alubosa elewe) from the Liliaceae family have been widely used in Nigeria for managing disorders. Despite its extensive use in traditional medicine, there is no scientific validation to support this traditional use. Aim: This study aimed to evaluate the hepatoprotective effect of Allium ascalonicum methanol extract (A. AMLE) in albino rats with acetaminophen-induced liver injury. Methods: Standard procedures were employed to conduct a phytochemical screening of A. AMLE. The hepatoprotective effect of A. AMLE was assessed using biomarkers of hepatocellular indices, oxidative stress, and lipid peroxidation. Albino rats were administered distilled water or A. AMLE at doses of 100, 200, or 400 mg/kg body weight, respectively, for seven days. The treatment groups were challenged with 300 mg/kg body weight of acetaminophen on the sixth day. Results: The phytochemical screening revealed the presence of flavonoids, saponins, anthraquinones, alkaloids, and tannins in A. AMLE. Oral administration of A. AMLE significantly (p &lt; 0.05) attenuated acetaminophen-induced alterations in serum alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin, and total bilirubin levels. Additionally, the acetaminophen-mediated decrease in the activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase were significantly ameliorated in the liver of albino rats. Elevated levels of malondialdehyde, conjugated dienes, lipid hydroperoxides, tumor necrosis factor-α, and interleukins-6 and -8 were significantly reduced by A. AMLE. Conclusion: This study scientifically validates the traditional use of Allium ascalonicum leaves for liver disorder management by demonstrating that its methanol extract mitigates acetaminophen-induced hepatotoxicity through enhanced antioxidant enzyme activity, highlighting its potential as a natural hepatoprotective agent and paving the way for further research and clinical application.

Effects of seasonal variation on phytochemicals contributing to the antimalarial and antitrypanosomal activities of Breonadia salicina using a metabolomic approach

This study involves the investigation of various plant parts of Breonadia salicina (Vahl) Hepper and J.R.I. Wood across multiple consecutive seasons. It aims to delve into the phytochemistry of these different plant parts and establish connections between the findings and their biological activities. This comprehensive approach employs metabolomics techniques, with the ultimate goal of exploring the potential for drug development. Samples were collected in Fondwe, a village in Limpopo (South Africa), based on local reports of the efficacy of this plant used by traditional healers in the area. The antimalarial and antitrypanosomal activities of samples collected over the seasons were determined with the parasite lactate dehydrogenase (pLDH) and specific Trypanosoma brucei assays, respectively. Consequently, a total of 24 compounds were tentatively identified through ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Chemical profiles of the different plant parts of Breonadia salicina collected in different seasons produced contrasting metabolic profiles. Chemometric analysis of the UPLC-QTOF-MS data enabled us to determine the chemical variability of the crude stem bark, root and leaf extracts (n = 48) collected over four consecutive seasons by evaluating the metabolomics fingerprinting of the samples using an untargeted approach. Principal component analysis (PCA), hierarchical cluster analysis (HCA), and partial least squares discriminant analysis (PLS-DA) indicated the existence of two key clusters that are linked to the root, stem bark, and leaves. The stem and root chemistry differed from that of the leaves. Seasonal variations were noted in each plant part, with autumn and winter samples closely grouped compared to spring and summer samples in the methanol leaf extracts. Biochemometric analysis could not relate specific compounds to the antimalarial and antitrypanosomal activities of the active extracts, underscoring the intricate interactions among the secondary metabolites. This study further confirms the optimal plant parts to collect in each season for the most effective antimalarial and antitrypanosomal activities.

The Potential Role of Phytochemicals of Juniperus procera in the Treatment of Ovarian Cancer and the Inhibition of Human Topoisomerase II Alpha Activity.

A variety of active chemicals found in medicinal plants can be used to develop new medications with few adverse effects. In vitro and in silico analyses were used to evaluate the anticancer properties of Juniperus procera fruit and leaf extracts. Here, we show that the methanolic extract from J procera fruit and leaf extracts inhibits 2 human ovarian cancer cell lines, A2780CP and SKOV-3. The leaf extract demonstrated strong cytotoxicity against A2780CP with an IC50 of 1.2 μg/mL, almost matching the IC50 of the anticancer medication doxorubicin (0.9 μg/mL). Higher antioxidant activity was observed in the fruit than leaf extract. The molecular docking results showed that the active component, podocarpusflavone A, was the best-docked chemical with the human topoisomerase II alpha enzyme. According to our knowledge, this is the first in vitro study to show the cytotoxicity of J procera extracts against the 2 previously described human ovarian cancer cell lines. The fact that the podocarpusflavone A molecule may have an inhibitory effect on the human topoisomerase II alpha enzyme was also revealed by this first in silico analysis. Our findings imply that the J procera fruit and leaf methanolic extract has anticancer characteristics that may guide future in vivo studies.