LE Diet Research Articles

Relationship between feed efficiency and gut microbiota in laying chickens under contrasting feeding conditions

The gut microbiota is known to play an important role in energy harvest and is likely to affect feed efficiency. In this study, we used 16S metabarcoding sequencing to analyse the caecal microbiota of laying hens from feed-efficient and non-efficient lines obtained by divergent selection for residual feed intake. The two lines were fed either a commercial wheat-soybean based diet (CTR) or a low-energy, high-fibre corn-sunflower diet (LE). The analysis revealed a significant line x diet interaction, highlighting distinct differences in microbial community composition between the two lines when hens were fed the CTR diet, and more muted differences when hens were fed the LE diet. Our results are consistent with the hypothesis that a richer and more diverse microbiota may play a role in enhancing feed efficiency, albeit in a diet-dependent manner. The taxonomic differences observed in the microbial composition seem to correlate with alterations in starch and fibre digestion as well as in the production of short-chain fatty acids. As a result, we hypothesise that efficient hens are able to optimise nutrient absorption through the activity of fibrolytic bacteria such as Alistipes or Anaerosporobacter, which, via their production of propionate, influence various aspects of host metabolism.

Effects of electrolyte balance on intestinal barrier, amino acid metabolism, and mTORC1 signaling pathway in piglets fed low-protein diets

A proper dietary electrolyte balance (dEB) is essential to ensure optimal growth performance of piglets. In the low-protein diet, this balance may be affected by the reduction of soybean meal and the inclusion of high levels of synthetic amino acids. The objective of this experiment was to evaluate the optimal dEB of low-protein diets and its impact on the growth performance of piglets. A total of 108 piglets (initial age of 35 d) were randomly divided into 3 groups with 6 replicates of 6 pigs each as follows: low electrolyte diet (LE group; dEB = 150 milliequivalents [mEq]/kg); medium electrolyte diet (ME group; dEB = 250 mEq/kg); high electrolyte diet (HE group; dEB = 350 mEq/kg). Results indicated that the LE and HE diet significantly decreased the average daily gain, average daily feed intake, and crude protein digestibility (P < 0.05) in piglets. Meanwhile, LE diets disrupted the structural integrity of the piglets' intestines and decreased jejunal tight junction protein (occludin and claudin-1) expression (P < 0.05). Additionally, the pH and HCO3− in the arterial blood of piglets in the LE group were lower than those in the ME and HE groups (P < 0.05). Interestingly, the LE diet significantly increased lysine content in piglet serum (P < 0.05), decreased the levels of arginine, leucine, glutamic acid, and alanine (P < 0.05), and inhibited the mammalian target of rapamycin complex 1 (mTORC1) pathway by decreasing the phosphorylation abundance of key proteins. In summary, the dietary electrolyte imbalance could inhibit the activation of the mTORC1 signaling pathway, which might be a key factor in the influence of the dEB on piglet growth performance and intestinal health. Moreover, second-order polynomial (quadratic) regression analysis showed that the optimal dEB of piglets in the low-protein diet was 250 to 265 mEq/kg.

Research Note: Comparative effects of liquid and dry applications of a combination of lysolecithin, synthetic emulsifier, and monoglycerides on growth performance, nutrient digestibility, and litter moisture in broilers fed diets of differing energy density

Supplementation of a combination of lysolecithin, a synthetic emulsifier, and monoglycerides (LEX) in liquid and dry form to broiler diets with different energy levels was investigated to determine their effect on performance, litter quality and subsequent occurrence of footpad lesions. One thousand two hundred and forty-eight-day-old Ross 308 broilers were assigned to 1 of 6 treatments for a 42-day study: a basal diet with a normal energy content (NE); NE + 300 g/t LEX in liquid form (LEL); NE + 500 g/t LEX in dry form (LED); a basal diet with low energy (LE, -90 kcal/kg starter, -100 kcal/kg grower, finisher), LE + 300 g/t LEL and a LE + 500 g/t LED. Each treatment consisted of 13 pens of 16 birds each. Diets were fed in 3 phases (starter d 0-10, grower d 11-21, finisher d 22-42). Feed intake and weight were measured on d 0, 10, 21, and 42. On d 42 a litter sample was collected from each pen and 2 birds per pen were assessed for footpad lesions and breast scald. Data were analyzed using JMP 16, with means separation achieved using Tukey's HSD; significance was assumed at P < 0.05. Results showed a higher (P < 0.05) cumulative bodyweight gain with LEX supplementation (NE CON = 2,718 g, NE+LED = 2,829, NE+LEL = 2,895, LE CON = 2,722, LE+LED = 2,787, LE+LEL = 2,893; P = 0.0027). An increased feed intake was observed for the LE diets, however cumulative FCR of LE+LED and LE+LEL remained equal to the NE control (1.657 NE CON, 1.657 LE+LED, 1.623 LE+LEL; P > 0.05), suggesting LEX enabled the birds to compensate for the energy gap. Litter dry matter was significantly improved with both LED and LEL supplementation compared to the control groups, and resulted in lower (P < 0.05) occurrence and severity of footpad lesions and breast scalds. Considering the income over feed cost (IOFC) of the NE treatment as the reference point for comparison, all other treatments improved profitability, with NE+LEL and LE+LEL achieving the greatest IOFC with 154.58 and 175.96 €/1,000 birds respectively. In conclusion, feeding broilers a combination of lysophospholipids, a synthetic emulsifier and monoglycerides resulted in improved bird performance. The use of the LEX also improved litter quality and footpad health, therefore improving animal welfare indicators such as breast scald and footpad measurements.

Effect of Prepartum Dietary Energy Level on Production and Reproduction in Nili Ravi Buffaloes.

Simple SummaryThe objective was to evaluate the effect of prepartum dietary energy level on performance of Nili Ravi buffaloes. Buffaloes were fed low, medium or high energy diet during 63 days prepartum and same lactation diet during 14 weeks postpartum. Buffaloes fed the high energy diet had better performance compared with buffaloes fed the medium or low energy diet. While, prepartum feeding of low energy diet adversely affected buffalo performance.The objective of this study was to evaluate the effect of prepartum dietary energy level on postpartum production and reproduction in Nili Ravi buffaloes (n = 21). The buffaloes were offered low energy (LE: 1.31 Mcal/kg DM NEL (net energy for lactation)), medium energy (ME: 1.42 Mcal/kg DM NEL) or high energy (HE: 1.54 Mcal/kg DM NEL) diet for 63 days prepartum, and received the same lactation diet (LD: 1.22 Mcal/kg DM NEL) during 14 weeks postpartum. The effects of dietary energy level and week were analyzed with Proc GLIMMIX model. Dry matter intake (DMI) was lower in buffaloes fed the LE diet compared with buffaloes fed the ME or HE diet. Calf birth weight (CBW) was higher in buffaloes fed the HE diet compared with buffaloes fed the ME or LE diet. Milk production was similar in buffaloes fed the HE, ME or LE diet within 14 weeks postpartum and throughout the lactation. Milk fat was higher in buffaloes fed the LE diet compared with buffaloes fed the ME or HE diet. Milk protein and lactose yields was high in buffaloes fed the HE diet compared with buffaloes fed the ME or LE diet. Body condition score was high in HE and was affected by diet × week interactions during pre and postpartum period. The concentrations of β-hydroxybutyrate (BHBA) and triglycerides in serum was lowest in buffaloes fed the HE diet compared with the buffaloes fed the ME or LE diet. The buffaloes fed the HE diet had early uterine involution (UI), first estrus, short dry days, and calving interval (CI) compared with buffaloes fed the ME or LE diet. None of buffaloes fed the LE diet exhibited estrus during the first 14 weeks postpartum compared with buffaloes fed the ME or HE diet. In conclusion, prepartum feeding of high energy diet can be helpful in improving the postpartum productive and reproductive performance in Nili Ravi buffaloes.

105 The Impact of Lowering Floor Space Allowance and Dietary Energy Level on Finishing Pigs, from 65 Kg to Market, on Growth Performance

Abstract The objective of this experiment was to evaluate the impact of reducing floor space allowance in finishing hogs with a starting body weight (BW) of 65 ± 2 kg when fed high versus low energy diets on growth performance. Mixed-sex pens (n=88) with 24 ± 1 pigs per pen were blocked by starting BW to a randomized complete block design with eight treatments. These eight treatments formed a 2 x 4 factorial arrangement with 2 energy levels: low (LE, 3270 ±19 kcal/kg ME) vs. high (HE, 3392 ±19 kcal/kg ME) accomplished through fat inclusion; and 4 floor space allowances: 0.60, 0.63, 0.65, 0.67 m2 per pig. Assigned floor space was adjusted by moveable gates in the rear of the pen, which were corrected at each pig removal until the marketing phase. Pen weight and feed disappearance were measured for 2 phases at d 0, 29, and 48 to calculate average daily gain (ADG), average daily feed intake (ADFI), and gain to feed ratio (GF). Data were analyzed by pen (SAS 9.4, Cary, NC) with the repeated measure of phase, and the fixed effects of floor space allowance, dietary energy, and the interaction between. For the overall trial, decreased floor space had no effect (P &amp;gt; 0.1) on ADG, ADFI, or GF (0.36, for all). Energy had a significant effect (P &amp;lt; 0.01) on ADFI (3.17 vs 3.12 kg for LE and HE, respectively) and GF (0.35 and 0.36 for LE and HE, respectively), and tended to impact ADG (1.12 vs 1.13 kg, for LE and HE, respectively; P = 0.08). In conclusion, lowering floor space allowance from 0.67 m2 down to 0.60 m2 did not affect the growth performance of pigs starting at 65 kg. Pigs fed HE diets had 2.86% better GF compared with pigs fed LE diets, however had similar growth and body weight.

Effect of diet energy level and genomic residual feed intake on bred Holstein dairy heifer growth and feed efficiency

The objective of this study was to determine growth, feed intake, and feed efficiency of postbred dairy heifers with different genomic residual feed intake (RFI) predicted as a lactating cow when offered diets differing in energy density. Postbred Holstein heifers (n = 128, ages 14-20 mo) were blocked by initial weight (high, medium-high, medium-low, and low) with 32 heifers per block. Each weight block was sorted by RFI (high or low) to obtain 2 pens of heifers with high and low genomically predicted RFI within each block (8 heifers per pen). Low RFI heifers were expected to have greater feed efficiency than high RFI heifers. Dietary treatments consisted of a higher energy control diet based on corn silage and alfalfa haylage [HE; 62.7% total digestible nutrients, 11.8% crude protein, and 45.6% neutral detergent fiber; dry matter (DM) basis], and a lower energy diet diluted with straw (LE; 57.0% total digestible nutrients, 11.7% crude protein, and 50.1% neutral detergent fiber; DM basis). Each pen within a block was randomly allocated a diet treatment to obtain a 2 × 2 factorial arrangement (2 RFI levels and 2 dietary energy levels). Diets were offered in a 120-d trial. Dry matter intake by heifers was affected by diet (11.0 vs. 10.0 kg/d for HE and LE, respectively) but not by RFI or the interaction of RFI and diet. Daily gain was affected by the interaction of RFI and diet, with low RFI heifers gaining more than high RFI heifers when fed LE (0.94 vs. 0.85 kg/d for low and high RFI, respectively), but no difference for RFI groups when fed HE (1.16 vs. 1.19 kg/d for low and high RFI, respectively). Respective feed efficiencies were improved for low RFI compared with high RFI heifers when fed LE (10.6 vs. 11.8 kg of feed DM/kg of gain), but no effect of RFI was found when fed HE (9.4 vs. 9.5 kg of DM/kg of gain for high and low RFI, respectively). No effect of RFI or diet on first-lactation performance through 150 DIM was observed. Based on these results, the feed efficiency of heifers having different genomic RFI may be dependent on diet energy level, whereby low RFI heifers utilized the LE diet more efficiently. The higher fiber straw (LE) diet controlled intake and maintained more desirable heifer weight gains. This suggests that selection for improved RFI in lactating cows may improve feed efficiency in growing heifers when fed to meet growth goals of 0.9 to 1.0 kg of gain/d.

Dietary Energy Level Impacts the Performance of Donkeys by Manipulating the Gut Microbiome and Metabolome.

Considerable evidence suggests that dietary energy levels and gut microbiota are pivotal for animal health and productivity. However, little information exists about the correlations among dietary energy level, performance, and the gut microbiota and metabolome of donkeys. The objective of this study was to investigate the mechanisms by which dietary energy content dictates the growth performance by modulating the intestinal microbiome and metabolome of donkeys. Thirty-six nine-month-old male Dezhou donkeys with similar body weights were randomly assigned to two groups fed low- or high-energy diets (LE or HE). The results showed that donkeys fed HE had increased (p < 0.05) the average daily gain (ADG) and feed efficiency (G/F) compared with those that received LE diet. The gut microbiota in both groups was dominated by the phyla Firmicutes and Bacteroidetes regardless of the dietary energy level. However, feeding HE to donkeys significantly decreased (p < 0.05) the ratio of Firmicutes to Bacteroidetes (F/B). Compared to the LE group, feeding HE specifically increased the abundances of unidentified_Prevotellaceae (p = 0.02) while decreasing the richness of unidentified_Ruminococcaceae (p = 0.05). Compared to the LE group, feeding the HE diet significantly (p < 0.05) upregulated certain metabolic pathways involving the aspartate metabolism and the urea cycle. In addition, the increased bacteria and metabolites in the HE-fed group exhibited a positive correlation with improved growth performance of donkeys. Taken together, feeding the HE diet increased the richness of Prevotellaceae and upregulated growth-related metabolic pathways, which may have contributed to the ameliorated growth performance of donkeys. Thus, it is a recommendable dietary strategy to feed HE diets to fattening donkeys for superior product performance and feed efficiency.

De-oiled soy lecithin positively influenced growth performance, nutrient digestibility, histological intestinal alteration, and antioxidant status in turkeys fed with low energy diets

ABSTRACT 1. The purpose of this research was to investigate the effects of supplementing an emulsifier (de-oiled soybean lecithin (DSL)) in a low metabolisable energy (ME) diet on growth performance, nutrient digestibility, carcase characteristics, intestinal morphology, blood metabolites, and antioxidant status in growing turkeys. 2. A total of 480 one-day-old turkeys were assigned to one of four dietary treatments with of eight replicates of 15 birds each. Experimental treatments included a basal diet (BE) with commercially recommended levels of ME, a reduced energy diet (RE) with 0.42 MJ/kg reduction in dietary ME content, the RE diet + 1 g/kg DSL (DSL-1), and RE + 2 g/kg DSL (DSL-2). 3. After 112 days, the body weight, average daily gain, and feed:gain in turkeys fed the supplemented for BE diets were better (P < 0.05) than in those fed RE, and those fed diet DSL-2 had the best performance. Although the RE diet decreased abdominal fat and relative liver weight (P < 0.05), compared to the BE diet, and supplementation with either level of DSL did not influence these variables. 4. There were linear increases (P < 0.05) in fat digestibility, nitrogen-corrected apparent ME, and duodenal villus height, villus height/crypt depth ratio, and villus surface area in LE diet supplemented with DSL. From the jejunal morphology, crypt depth was decreased by DSL-supplemented diets (P < 0.05). 5. Serum triglyceride, total cholesterol, and malondialdehyde concentrations were lower, whereas the serum superoxide dismutase activity was greater for the DSL-2 group compared to the BE and RE groups (P < 0.05). 6. The findings suggested that, while low-ME diets impaired turkey growth performance, dietary supplementation of DSL could reverse such impacts of these diets. The DSL-supplemented diet at the inclusion level of 2 g/kg was advantageous over both BE and RE diets in terms of intestinal morphology, lipid profile, and antioxidant status in growing turkeys.

Impact of varying starter amino acid density and energy on 42 d male Cobb MV × Cobb 500 broiler performance and processing

Summary Previous research has shown that feeding high amino acid density (AAD) diets or increased AME improved broiler performance, though the relationship between AAD and AME on performance needs to be further explored. Therefore, the objective of this study was to evaluate the impact of feeding 2 AAD and 4 AME levels from day 0 to 14 on performance and yield of 42-day-old Cobb MV × Cobb 500 males. Starter diets were formulated to medium or high AAD (HAA), and very low (VLE), low (LE), medium (ME), or high (HE) AME. Common diets were provided from day 15 to 41. A 2 × 4 factorial arrangement of treatments was used, with day 0 BW considered as a covariant. On day 0 to 14, birds receiving HAA had the lowest feed conversion ratio (FCR) corrected and uncorrected for mortality (uFCR). Birds receiving VLE diets had the highest day 0 to 14 FCR when compared to birds fed other AME levels. Feeding LE diets resulted in higher day 0 to 14 FCR when compared to ME and HE diets. An AAD × AME interaction for day 0 to 14 total Lys intake/bird found that Lys intake/bird decreased when increasing dietary AME for HAA diets. An AAD × AME interaction was observed for day 0 to 28 uFCR whereas increasing starter AAD in diets formulated to ME and HE increased day 0 to 28 uFCR; no change was observed for VLE and LE diets. However, overall performance and processing was not affected by varying starter AAD and AME levels. Further research should investigate similar feeding strategies, but in other feeding phases.

Dietary energy sources and levels shift the multi-kingdom microbiota and functions in the rumen of lactating dairy cows

BackgroundDietary energy source and level in lactation diets can profoundly affect milk yield and composition. Such dietary effects on lactation performance are underpinned by alteration of the rumen microbiota, of which bacteria, archaea, fungi, and protozoa may vary differently. However, few studies have examined all the four groups of rumen microbes. This study investigated the effect of both the level and source of dietary energy on rumen bacteria, archaea, fungi, and protozoa in the rumen of lactating dairy cows. A 2 × 2 factorial design resulted in four dietary treatments: low and high dietary energy levels (LE: 1.52–1.53; and HE: 1.71–1.72 Mcal/kg dry matter) and two dietary energy sources (GC: finely ground corn; and SFC: steam-flaked corn). We used a replicated 4 × 4 Latin square design using eight primiparous Chinese Holstein cows with each period lasting for 21 d. The rumen microbiota was analyzed using metataxonomics based on kingdom-specific phylogenetic markers [16S rRNA gene for bacteria and archaea, 18S rRNA gene for protozoa, and internally transcribed spacer 1 (ITS1) for fungi] followed with subsequent functional prediction using PICRUSt2.ResultsThe GC resulted in a higher prokaryotic (bacterial and archaeal) species richness and Faith’s phylogenetic diversity than SFC. For the eukaryotic (fungi and protozoa) microbiota, the LE diets led to significantly higher values of the above measurements than the HE diets. Among the major classified taxa, 23 genera across all the kingdoms differed in relative abundance between the two dietary energy levels, while only six genera (none being protozoal) were differentially abundant between the two energy sources. Based on prokaryotic amplicon sequence variants (ASVs) from all the samples, overall functional profiles predicted using PICRUSt2 differed significantly between LE and HE but not between the two energy sources. FishTaco analysis identified Ruminococcus and Coprococcus as the taxa potentially contributing to the enriched KEGG pathways for biosynthesis of amino acids and to the metabolisms of pyruvate, glycerophospholipid, and nicotinate and nicotinamide in the rumen of HE-fed cows. The co-occurrence networks were also affected by the dietary treatments, especially the LE and GC diets, resulting in distinct co-occurrence networks. Several microbial genera appeared to be strongly correlated with one or more lactation traits.ConclusionsDietary energy level affected the overall rumen multi-kingdom microbiota while little difference was noted between ground corn and steam-flaked corn. Some genera were also affected differently by the four dietary treatments, including genera that had been shown to be correlated with lactation performance or feed efficiency. The co-occurrence patterns among the genera exclusively found for each dietary treatment may suggest possible metabolic interactions specifically affected by the dietary treatment. Some of the major taxa were positively correlated to milk properties and may potentially serve as biomarkers of one or more lactation traits.

Effects of free feeding time system and energy level to improve the reproductive performance of lactating sows during summer.

The reproductive performance of lactating sows was investigated by using different feeding methods including conventional feeding (CF, 3 times/d) or free feeding (FF), and different dietary energy level including low energy (LE: 3,300) or high energy (HE: 3,400 kcal/kg) during the hot season. A total of twenty-eight crossbred (Yorkshire × Landrace) sows were distributed into four treatments as a 2 × 2 factorial arrangement. Sows in the FF group showed lower body weight and backfat loss (p < 0.05) compared with the CF group. Backfat loss during lactation was lower (p < 0.05) in sows fed HE diet than in that fed LE diet. There were no significant differences in litter survival rate and weaning to estrus interval, but the litter weight at weaning was improved (p < 0.05) in FF and HE sows. Hence, it is concluded that using the free-feeding system or increased dietary energy density leads to improved sow performance during hot ambient temperature.

Increasing the ratio of SID lysine to metabolizable energy improves pig performance during a viral challenge.

Porcine reproductive and respiratory syndrome virus (PRRSV) compromises pig performance. However, increasing standardized ileal digestible Lys per Mcal metabolizable energy (SID Lys:ME) above requirement has been shown to mitigate reduced performance seen during a porcine reproductive and respiratory syndrome (PRRS) virus challenge. The objective of this study was to evaluate the effects of increasing the dietary SID Lys:ME from 100% National Research Council (NRC) requirement to 120% of the requirement in vaccinated (vac+; modified live vaccine Ingelvac PRRS) and non-vaccinated (vac−; no PRRS vaccine) grower pigs subjected to a PRRSV challenge. In addition, the dietary formulation approach to achieve the 120% ratio by increasing Lys relative to energy (HL) or diluting energy in relation to Lys (LE) was evaluated. This allowed us to test the hypothesis that pigs undergoing a health challenge would have the ability to eat to their energy needs. Within vaccine status, 195 mixed-sex pigs, vac+ (35.2 ± 0.60 kg body weight [BW]) and vac− (35.2 ± 0.65 kg BW) were randomly allotted to one of three dietary treatments (2.67, 3.23, or 3.22 g SID Lys:ME) for a 42-d PRRS virus challenge study representing 100%, 120%, and 120% of NRC requirement, respectively. Pigs were randomly allotted across two barns, each containing 24 pens with 7 to 10 pigs per pen (8 pens per diet per vaccine status). On day post-inoculation 0, both barns were inoculated with PRRSV and started on experimental diets. Within vaccine status, weekly and overall challenge period pig performance were assessed. In both vac+ (P < 0.05) and vac− (P < 0.05) pigs, the HL and LE diets increased end BW and overall average daily gain (ADG) ADG compared with pigs fed the control diet (P < 0.05). Overall, average daily feed intake (ADFI) during the challenge period was greater (P < 0.05) for pigs fed the LE diet compared with pigs fed control and HL treatments, regardless of vaccine status (20% and 17% higher ADFI than the control in vac+ and vac− pigs, respectively). The HL vac+ pigs had the greatest gain to feed (G:F) compared with the control and LE pigs (0.438 vs. 0.394 and 0.391 kg/kg, respectively; P < 0.01). Feed efficiency was not impacted (P > 0.10) by treatment in the vac− pigs. In summary, PRRSV-challenged grower pigs consumed feed to meet their energy needs as indicated by the increase in ADFI when energy was diluted in the (LE) diet, compared with control pigs. In both PRRS vac+ and vac− pigs subsequently challenged with PRRSV, regardless of formulation approach, fed 120% SID Lys:ME diets resulted in enhanced overall growth performance.

Effect of carbohydrases on growth performance, nutrient digestibility, blood profiles, and fecal microbiota in weanling pigs fed reduced energy diet

This study was conducted to evaluate the effect of carbohydrases on growth performance, nutrient digestibility, blood profiles, and fecal microbiota in weanling pigs fed reduced energy diet. A total of 160 crossbred [(Yorkshire × Landrace) × Duroc)] weanling pigs with an initial body weight (BW) of 7.23 ± 1.08 kg were used in this 42 d trial. Pigs were randomly allocated in one of the following four dietary treatments: (1) CON, basal diet [net energy (NE) 2545 kcal kg−1]; (2) LE, low-energy diet (NE 2470 kcal kg−1); (3) LEC1, LE + 0.1% carbohydrase; and (4) LEC2, LE + 0.2% carbohydrase based on the sex and BW. There were eight replications (pens) per treatment with five pigs (two male and three female) per pen. During day 0–7, pigs fed with LE diet had lower (P &lt; 0.05) average daily gain (ADG) and average daily feed intake than those fed with CON and LEC2 diets. LE group reduced (P &lt; 0.05) ADG throughout the experiment compared with CON and LEC2 groups. LE group reduced (P &lt; 0.05) the digestibility of dry matter, nitrogen, and gross energy compared with LEC2 diet. Taken together, LE diet caused detrimental effects on growth performance and nutrient digestibility in weanlings, whereas the addition of carbohydrases (0.2%) partially counteracted these negative effects.

Effects of lavender (Lavandula angustifolia) extract inclusion in diet on growth performance, innate immunity, immune-related gene expression, and stress response of common carp, Cyprinus carpio

In the present study, the effects of dietary lavender (Lavandula angustifolia) extract (LE) on growth performance and immune, antioxidant, and stress responses in common carp, Cyprinus carpio were investigated. For this, four diets containing 0, 0.5, 1.0 and 1.5% LE were offered to triplicate groups of fish (25.8 ± 1.66 g) for 70 days, and then the fish were exposed to a 3-h crowding stress. Growth performance, blood leukocyte count, plasma proteins and innate immune parameters, and head kidney cytokines’ gene expression were studied after the 70-day feeding trial (before the crowding stress). Plasma cortisol, glucose, superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were measured before and after the crowding stress. Results showed that dietary LE had no significant effects on the fish growth performance, blood leukocyte differential count, plasma albumin and total protein, and head kidney interferon gamma-1 gene expression. Blood leukocyte and plasma globulin, alternative complement and total immunoglobulin significantly increased in the fish fed with 1.0–1.5% LE diets compared to the fish fed with the control diet (0% LE). The plasma lysozyme activity significantly increased along with dietary LE supplemental levels. The lowest expression of interlukin-1 beta gene was related to the 1.0% LE treatment. Tumor necrosis factor-alpha gene expression significantly decreased along with increase in dietary LE levels and the lowest expression was related to the fish fed with 1.5% LE diet. The LE administration led to significant increase in interlukine-10 gene expression and the highest expression was related to the fish fed with 1.0% and 1.5% LE diets. The fish fed with 1.5% LE diet had significantly higher transforming growth factor-beta gene expression compared to the control group. Liver SOD and CAT activities showed significant increases along with dietary LE supplementation levels. After the crowding stress, hepatic SOD decreased in the control group, but not the LE-supplemented groups. Stress led to significant decrease in hepatic CAT activities in all treatments, but CAT activity increased along with the LE levels with highest activity in the fish fed with 1.5% LE. The stress led to significant increase in hepatic MDA in the treatments control and 0.5% LE, but not 1.0–1.5% LE groups. The stress led to significant increase in plasma cortisol and glucose levels in all treatments but dietary LE levels significantly decreased these parameters. Dietary LE supplementation at levels of 1.0–1.5% is recommended for common carp to suppress stress, inflammation and oxidative conditions and augment immune responses in the fish.

Amino acid efficiencies of utilization vary by different mechanisms in response to energy and protein supplies in dairy cows: Study at mammary-gland and whole-body levels

Variations of mammary gland (MG) metabolism were studied in dairy cows in response to diets containing 2 levels of net energy of lactation [NEL; 25.0 and 32.5 Mcal/d for low (LE) and high energy (HE), respectively], combined with 2 levels of metabolizable protein [MP, 1,266 and 2,254 g/d of protein digestible in the intestine for low (LP) and high protein (HP), respectively] in a 2 × 2 factorial arrangement. Four cows received 4 diets (LELP, HELP, LEHP, and HEHP) in a 4 × 4 Latin square design with 2-wk experimental periods. Milk production and feed intake were measured on the last 5 d of each period, whereas MG net uptake of AA was determined on d 13. Efficiencies were estimated as the sum of measured milk true protein yield (MPY) and of estimations of metabolic fecal and scurf proteins multiplied by their respective AA profile and divided by the estimated AA supply minus the AA endogenous urinary loss. The increased MPY in the HE compared with the LE diets (higher by 123 g/d) was accompanied by increased mammary plasma flow and MG uptake of the nonessential AA (NEAA) and the essential AA (EAA), except for branched-chain AA. In contrast, the increase in MPY (higher by 104 g/d) observed in the HP compared with the LP diets was linked to increased MG uptake of EAA without a change in mammary plasma flow and a decreased NEAA uptake. Because MG uptake of total AA-N was almost equal to cows' milk output on a nitrogen basis, these different mechanisms involve a large MG flexibility, with variable synthesis of NEAA. In addition, MP efficiency did not increase only through increased MPY in the HE compared with the LE diets but also through metabolic fecal protein, estimated to increase (by 65 g/d) with dry matter intake. The MPY increased in the HP compared with the LP diets, but the increase was smaller than the calculated increase (greater by 993 g/d) in MP supply. The highest MG clearance rates of individual EAA could suggest that Met, His, and Lys were limiting in LP, and Met was the most limiting AA in HP. Interestingly, a similar hypothesis could be stated by analyzing estimated AA efficiencies. The highest efficiencies among EAA, observed for His in HELP and for Met with the other diets, could indicate that they were the most limiting AA in these respective diets, whereas other EAA (including Lys) efficiencies varied with MP efficiency. The MG metabolic flexibility with regard to individual AA utilization partially contributes to the anabolic fate of AA through MPY; however, other export proteins also contribute to variations in MP and AA efficiencies.

Effects of dietary supplementation of β-mannanase on performance and egg quality in laying hens

The objective of the experiment was to evaluate effects of dietary supplementation of β-mannanase (Hemicell®) on productive performance, egg quality, and fecal moisture content in laying hens from 20 to 35 weeks of age. A total of 375 Isa Brown’s hens (1615.6 ± 76.4 g/bird) were randomly assigned to 5 treatments in a completely randomized design. The 5 dietary treatments included (1) basal diet with a level of 2800 kcal ME and no β-mannanase supplementation (HE, Control), (2) HE + 32 units of β-mannanase/g of feed, (3) HE + 64 units of β-mannanase/g of feed, (4) basal diet with a level of 2700 kcal ME (LE) + 32 units of β-mannanase/g of feed, and (5) LE + 64 units of β-mannanase/g of feed. Each treatment was replicated with 25 cages of 3 hens each. All diets were in meal form and contained no antibiotics. The addition of β-mannanase to HE diets did not affect the egg production of birds as compared with the control (P &gt; 0.05). The birds fed LE diets with β-mannanase had the same egg production as those fed the control and β-mannanase-supplemented HE diets (P &gt; 0.05). Differences in egg weight, egg quality, survival rate, and fecal moisture content were not significant among the treatments (P &gt; 0.05). Briefly, addition of β-mannanase (32 units/g of feed) to LE diets would be beneficial for layers during the early laying period as it resulted in the same performance and egg quality as the HE diets without β-mannanase supplementation.

Effects of phytonutrients on growth performance, antioxidative status, and energy utilization of broilers fed low energy diets.

Two experiments were conducted to investigate the effects of phytonutrients (PN) on growth performance, antioxidant status, intestinal morphology, and nutrient utilization of birds fed low energy diets. In Exp. 1, a total of 1,440 one-day-old Ross 308 male broiler chickens were randomly divided into 3 treatment groups, with 16 replicates per treatment (48 pens; 30 birds per pen). Birds in treatment 1 were fed diets with normal energy content (NE). Birds in treatment 2 were fed NE diet but with 60 kcal removed (LE). Birds in treatment 3 were assigned to LE diet supplemented with PN (LE + PN). Results indicated that LE diet increased feed conversion ratio (FCR) compared with NE from d 1 to 38, while LE + PN diet prevented this response (P = 0.02). At d 26, birds in the LE + PN group had the highest ileal and jejunal villus height to crypt depth (VH:CD) ratio. At d 39, PN supplementation improved ileal and jejunal VH:CD ratio, compared with LE group. Moreover, birds fed PN diets received a better economic profit. In Exp. 2, 360 one-day-old Ross 308 male broiler chickens were used in a metabolism study. The treatments used in Exp. 2 were the same as those in Exp.1, with 4 replicates (pens) and 30 birds in each replicate. Dietary apparent metabolism energy (AME), energy and protein digestibility were determined between 21 and 28 d of age. Results showed that chickens fed LE + PN diet tended to have greater AME (P = 0.02) and nitrogen-corrected apparent metabolism energy (AMEn) (P = 0.03) than birds fed LE diets. It was concluded that LE + PN showed a potential advantage to improve feed conversion and gut health of broilers, as well as economic profits.

Effect of diet energy density and genomic residual feed intake on prebred dairy heifer feed efficiency, growth, and manure excretion

The objective of this study was to determine the growth, feed efficiency, and manure excretion of prebred dairy heifers with differing predicted genomic residual feed intakes (RFI) when offered diets differing in energy density. Prebred Holstein heifers (n = 128, ages 4 to 8 mo) were blocked by weight (low, medium-low, medium-high, or high) with 32 heifers per block. Heifers in each weight block were grouped by RFI and randomly assigned to obtain 2 pens of high (HRFI) and 2 pens of low RFI (LRFI) heifers within each block (8 heifers/pen). Heifers with LRFI were hypothesized to have greater feed efficiency than HRFI heifers. Dietary treatments were a high-energy diet (HE; 66.6% total digestible nutrients, 14.0% crude protein, and 36.3% neutral detergent fiber, dry matter basis) and a low-energy diet (LE; 63.8% total digestible nutrients, 13.5% crude protein, and 41.2% neutral detergent fiber, dry matter basis). Each pen of heifers was randomly assigned to a treatment to obtain a 2 × 2 factorial arrangement (2 RFI levels × 2 diet energy densities). Diets were offered in a 120-d trial. Dry matter intake was not affected by diet, RFI, or their interaction. Average daily gain (ADG) was affected by diet, with heifers fed HE having greater ADG than heifers fed LE. In addition, RFI affected ADG, with LRFI heifers having greater ADG than HRFI heifers, whereas the interaction of RFI and diet was not significant. Feed efficiency was improved for heifers fed the HE diet, but it was not affected by RFI or the interaction of RFI and diet. Overall, feed efficiency of prebred heifers was not dependent on predicted genomic RFI, because the greater ADG of LRFI heifers was accompanied by slightly higher dry matter intake. Feed efficiency of heifers was reduced when heifers were fed the LE diet, but this resulted in more optimal ADG compared with the HE diet fed for ad libitum intake.

Breed and Nutrition Effects on Meat Quality and Retail Color after Lamb Pre-Slaughter Stress

The main objective of this research was to investigate the interactions between breed, nutrition and pre-slaughter stress, and the potential impacts on the meat quality and retail color/shelf life of lamb meat. Forty-eight Merino and second cross (Merino × Border Leicester) × Poll Dorset lambs were allocated to 2 dietary treatments: low energy diet (LE; 7.8 MJ ME/kg and 12% CP) vs. high energy (HE; 11.8 MJ ME/kg and 12% CP). Samples of longissimus lumborum (LL), and rectus femoris (RF) were taken 15 min post-slaughter for glycogen and lactate determination. pH and temperature of muscle samples were recorded at 0.5, 1.5, 3, 4.5, 6, and 24 h post-mortem. Muscle LL samples from the same side were collected at 24 h post slaughter for tenderness, cooking loss and retail color assessment. For retail color stability, the selected portion of muscle LL was sliced to create a fresh surface, placed on a tray and over wrapped. Color measurements were made fresh (after a 30 min bloom at 4°C, d 1) and then on d 3 and 6 of display, using a Hunter lab spectrophotometer XE Plus. Second-cross lambs on HE had the highest muscle glycogen at slaughter for both LL and RF. For the RF, the pHu values were elevated in Merinos on both LE and HE diets and 2X on the LE diet. Only for 2X lamb was the HE diet sufficient to reduce the pHu. HE diet reduced blood lactate in 2X lambs and elevated in Merinos. Merinos produced tougher meat at 6 d post-slaughter, which was also reflected by higher cooking losses. Both breeds of lambs on the LE diet had longer color shelf-life (higher R630/580) than the lambs on the HE diets.

Effect of dietary β-mannanase on productive performance, egg quality, and utilization of dietary energy and nutrients in aged laying hens raised under hot climatic conditions.

ObjectiveThe objective of this experiment was to investigate the effect of dietary β-mannanase on productive performance, egg quality, and utilization of dietary energy and nutrients in aged laying hens raised under hot climatic conditions.MethodsA total of 320 84-wk-old Hy-line Brown aged laying hens were allotted to one of four treatments with eight replicates in a completely randomized design. Two dietary treatments with high energy (HE; 2,800 kcal/kg nitrogen-corrected apparent metabolizable energy [AMEn]) and low energy (LE; 2,700 kcal/kg AMEn) were formulated. Two additional diets were prepared by adding 0.04% (MN4) or 0.08% β-mannanase (MN8) to LE treatment diets. The feeding trial was conducted for 28 d, covering a period from July to August in South Korea. The average daily room temperature and relative humidity were 29.2°C and 83%, respectively.ResultsProductive performance, egg quality, and cloacal temperature were not influenced by dietary treatments. The measured AMEn values for MN8 diets were similar to those for HE diets, which were greater (p<0.05) than those for LE and MN4 diets. However, the AMEn values for MN8 diets did not differ from those for LE and MN4 diets.ConclusionThe addition of β-mannanase to low energy diets increases energy values for diets fed to aged laying hens. However, this increase has little positive impacts on performance and egg quality. These results indicate that dietary β-mannanase does not mitigate the heat stress of aged laying hens raised under hot climatic conditions.