Low-density lipoprotein (LDL) apheresis via membrane filtration process is an extracorporeal blood purification technique using for the treatment of patients suffering from familial hypercholesterolemia (FH) and severe coronary artery disease (CAD). As much as the importance of LDL separation, the maximum permeability of beneficial blood plasma components through the membrane is regarded as an important issue in this method. Herein, patterned membranes are produced by the effect of horizontally aligned microchannels that formed by the incorporation of sulfated chopped carbon fiber (SCCF) in the membrane structure. Characterization studies verified the binding of carrageenan as a sulfated polysaccharide on the surface of chopped carbon fiber (CCF). Transmission and reflective optical micrographs demonstrated the orientation of SCCFs in a horizontally aligned direction in the membrane structure. Surface analyses including water contact angle and zeta potential revealed the improvement of hydrophilicity and negative charge density of membranes containing SCCF, respectively. Moreover, membrane surface roughness was enhanced with the addition of SCCF that led to a better selectivity. With the enhancement of SCCF concentration, the ratio of molecular weight cut-off to molecular weight retention onset (MWCO/MWRO) that is a marker of membrane selectivity was decreased effectively. The morphology study displayed the formation of an interfacial distance between SCCF and the polymeric matrix that produced horizontally parallel microchannels in the membrane structure. Blood plasma filtration and cardiovascular disease (CVD) risk factors verified that the M−SCCF3 (containing 0.7 wt% SCCF) due to having desired morphology and characteristics had the best performance for the LDL separation, and permeability of beneficial plasma components like high-density lipoprotein (HDL), and albumin protein. Furthermore, cell cytotoxicity analysis revealed no cytotoxicity in membranes which endorsed desired interaction of developed membranes with L-929 cells as the representation of physiological ambient. Also, reduction of protein adsorption on the membrane surface, and prolongation of coagulation times endorsed the hemocompatibility of membranes. The overall results certified that the M−SCCF3 due to having the best performance and desired structural properties was the best membrane for use in LDL apheresis.