Extracellular Polymeric Substances Layer Research Articles

Bacterial Biofilm Formation on a Human Cochlear Implant

To report the characteristics of a bacterial biofilm from the surface of a cochlear implant. Bacterial biofilm formation on implanted devices causes intractable infections and device extrusions necessitating device removal, with loss of function. More information is needed about biofilm characteristics and interactions with the implant surface before better treatments can be designed. A retrospective case review was combined with a descriptive histological study of the surface of an otologic device. The receiver/stimulator device removed from a cochlear implant patient because of intractable infection and partial device extrusion was fixed and processed for microscopic examination. Its surface and the material present on its surface were analyzed using light and electron microscopy, focusing on surface texture, cell types, and bacteria species and extracellular polymeric substances present within the biofilm. Stereomicroscopic examination revealed extracellular polymeric substances, pinkish yellow in color, with spheres of uniform size scattered throughout, indicative of a biofilm containing Staphylococcus aureus. Biofilm density was greatest in depressions on the surface of the implant. Cross-sectional analysis revealed bacteria interspersed with polymorphonuclear leukocytes. Scanning electron microscopic examination demonstrated an amorphous layer of extracellular polymeric substances containing small filaments, bacteria, and inflammatory cells. Only Staphylococcus aureus was detected. Cochlear implant material can provide a surface for bacterial biofilm formation. Impressions can provide an environment conducive to biofilm establishment and growth, ultimately necessitating device removal, with loss of implant function. Biofilm characterization should aid in design of cochlear implant devices less susceptible to biofilm formation.

Influence of extracellular polymeric substances on deposition and redeposition of Pseudomonas aeruginosa to surfaces

In this study, the role of extracellular polymeric substances (EPS) in the initial adhesion of EPS-producing Pseudomonas aeruginosa SG81 and SG81R1, a non-EPS-producing strain, to substrata with different hydrophobicity was investigated. The release of EPS by SG81 was concurrent with a decrease in surface tension of a bacterial suspension from 70 to 45 mJ m(-2) that was absent for SG81R1. Both strains adhered faster and in higher numbers to a hydrophilic than to a hydrophobic substratum, but the initial deposition rates and numbers of adhering bacteria in a stationary-end point were highest for the non-EPS-producing strain SG81R1, regardless of substratum hydrophobicity. Both strains adhered less to substrata pre-coated with isolated EPS of strain SG81. Furthermore, it was investigated whether bacteria, detached by passing air-bubbles, had left behind 'footprints' with an influence on adhesion of newly redepositing bacteria. Redeposition on glass was highest for non-EPS-producing SG81R1 and decreased linearly with the number of times these cycles of detachment and deposition were repeated to become similar to the redeposition of SG81 after six cycles. This indicates that P. aeruginosa SG81 leaves the substratum surface nearly completely covered with EPS after detachment, while SG81R1 releases only minor amounts of surface active EPS, completely covering the substratum after repeated cycles of detachment and adhesion. Atomic force microscopy showed a thick and irregular EPS layer (up to 32 nm) after the first detachment cycle of EPS-producing strain SG81, whereas the putatively non-EPS-producing strain SG81R1 left a 9 nm thin layer after one cycle. X-ray photoelectron spectroscopy indicated that the bacterial footprints consisted of uronic acids, the prevalence of which increased with the number of detachment and deposition cycles.

Mecanismo de biolixiviación de sulfuros metálicos

Among the hydrometallurgical techniques for the treatment of sulfide mineral concentrates, bioleaching is one with a high application potential on the base of its advances widely recognized. In spite of it, its development at industrial level has been slow due to its main drawback: its low kinetics. The start-up of bioleaching processes needs necessarily a deeper understanding of the mechanisms involved in the dissolution of mineral sulfides, in order to control and improve industrial scale-up. This bibliographic review collects the present state on the subject through the fundamentals of bioleaching models which integrate both the chemical-electrochemical behavior and the role played by microorganisms on the interface solution-mineral. At the same time other important aspects included in this paper are: the latest findings on chemical and electrochemical parameters of the process, the importance of extracellular polymeric substances (EPS) excreted by leach bacteria during its adherence to mineral particles, and the function played by the ferric iron trapped within this layer of EPS.