Layer Chromatography Method For Estimation Research Articles

High-Performance Thin Layer Chromatography Method for Simultaneous Estimation of Water-Soluble Vitamins: Analytical Method Development, Validation and Applications

Vitamins are pivotal ingredients in dietary supplements and nutraceutical products. Since many vitamins are unstable and rapidly degraded, it’s crucial to keep track of their loss throughout processing and storage. Monitoring hydrophilic vitamins may be more crucial than monitoring those lipophilic vitamins because the body does not maintain them as well. There seems to be a need for an all-encompassing method to estimate hydrophilic vitamins. In this study, we have developed a high-performance thin layer chromatography method for estimation of four water-soluble vitamins simultaneously using HPTLC silica-gel 60GF254 plates and ethyl acetate: methanol: hydrochloric acid (0.1N) (8.5:1:0.5 v/v/v) as a mobile phase, which was successfully validated as per the ICH guideline. All the validation parameters were within the acceptable range established by the ICH guideline. The method is precise, reproducible, easy, reliable, and applicable for the estimation of water-soluble vitamins in marketed products. To best of our knowledge, it is simpler, less time-consuming, and more economical than other approaches that have been published for the same purposes

Validated High Performance Thin Layer Chromatography Method for Simultaneous Estimation for Gallic Acid and Quercetin in Polyherbal Blend and Their Quantitative Estimation

Simple, sensitive high performance thin layer chromatography method for the estimation of gallic acid and quercetin in in-house polyherbal blend has been developed and validated. Methanolic solution of herbal blend comprising of Emblica officinalis, Camellia sinensis and Garcinia cambogia was used for analysis. The sepa-ration was performed on TLC aluminum plates precoated with silica gel G60 F254 and toluene: ethyl ace-tate: formic acid (5:1.5:1 v/v/v) at 254 nm scanning wavelength. The system gave well resolved peaks for gal-lic acid and quercetin at Rf 0.14 and Rf 0.29 respectively. The method validated as per ICH Q2R1 guidelines which shows regression co-efficient 0.9939 for gallic acid and 0.9988 for quercetin in range of 2–6 μg/ml. Recovery of gallic acid and quercetin was found in range of 98–102 % which confirms the accuracy of meth-od. Precision study (interday & intraday) showed that the relative standard deviation is less than 2 %, show-ing method is well precise. Proposed validated HPTLC method is simple, precise, specific, robust and accu-rate, and could find application in routine quality-control analysis. The method was used for quantitative es-timation of gallic acid and quercetin in the polyherbal blend and was found as 1.648 % w/w and 3.165 % w/w respectively.

Development of Validated Stability-indicating High Performance Thin Layer Chromatography Method for Estimation of Rabeprazole Sodium and Aceclofenac in Bulk Drug

Objective: To develop a validated stability-indicating high performance thin layer chromatography method for the estimation of Rabeprazole Sodium (RZL) and Aceclofenac (ACF) in bulk drugs.
 Methods: A high performance thin layer chromatographic (HPTLC) method has been developed for the separation of RZL & ACF on plates precoated with aluminium back silica gel 60 F254. Different mobile phases were used on trial and error basis for separation of two drugs. The final mobile phase selected for analysis was toluene: ethyl acetate: methanol: acetic acid: ammonia in the ration of 6:4:1:0.2:0.1 (v/v). Both the drugs showed maximum absorbance at 279 nm which was selected as the detection wavelength throughout the experimental work. Developed method was validated as per ICH guidelines. Forced degradation of drugs was carried out under various stress conditions and HPTLC method was used for analysing the stability of drugs.
 Results: HPTLC method was successfully developed for separation of RZL and ACF with clear separation of bands of the drugs. Method validation after assessment of various parameters indicated low % RSD within an acceptable limit of < 2.0 and the stability studies indicated the satisfactory separation of both the drugs from that of degraded products with considerable % recovery profile.
 Conclusion: The developed method is rapid, reliable, precise, and reproducible and demonstrates the suitability of the method for stability determination of rabeprazole and aceclofenac.

Development and Validation of High-Performance Thin Layer Chromatography Method for Estimation of Rifabutin in Bulk and Formulation

Development and Validation of High-Performance Thin Layer Chromatography Method for Estimation of Rifabutin in Bulk and Formulation

A validated high-performance thin layer chromatography method for estimation of lornoxicam and paracetamol in their combined tablet dosage form

Introduction: Lornoxicam (LORN) a nonsteroidal antiinflammatory drug of oxicam class is marketed in combination with Paracetamol, a common analgesic for acute inflammatory disease of joints. Materials and Methods: LORN and Paracetamol (PCM) were estimated at 280 nm by densitometry using silica gel 60 F 254 as stationary phase and a premix of toluene: chloroform: methanol: formic acid (3:5:1.5:0.2 v/v/v/v) as mobile phase. The method was found linear in a range of 160–560 nanograms/spot for LORN and 10 000–35 000 nanograms/spot for PCM with a correlation coefficient >0.99 for both. Result: PCM and LORN were well resolved with Rf 0.57 ± 0.02 and 0.75 ± 0.02, respectively. Conclusions: The developed high-performance thin layer chromatography method was found to be simple, specific, precise, and reproducible and can be used for the routine estimation of LORN and PCM in the combined tablet dosage form, available in market.

High-performance thin layer chromatography method for estimation of andrographolide in herbal extract and polyherbal formulations

A new, simple, sensitive, precise and robust high-performance thin layer chromatographic (HPTLC) method was developed for the estimation of andrographolide in herbal extracts and pharmaceutical dosage forms. Analysis of andrographolide was performed on TLC aluminium plates pre-coated with silica gel 60F-254 as stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with chloroform:toluene:methanol (66:26:8, v/v/v) at room temperature (25±2°C). The Rf value of andrographolide was found to be 0.49. Camag TLC scanner III was used for spectrodensitometric scanning and analysis in absorbance mode at 229nm. The system was found to give compact spots for andrographolide (Rf value of 0.49). The data for calibration plots showed good linear relationship with r2=0.9986 in the concentration range of 200ng to 1000ng with respect to peak area. The present method was validated by precision, recovery, robustness and reproducibility according to ICH guidelines. The limits of detection and quantification were determined and it was found to be 3.5 and 11.7ng, respectively. Statistical analysis of the data showed that the method is reproducible and selective for estimation of andrographolide.