Lavender Oil Research Articles

In vitro efficacy of lavender oil, otological gel and gentamicin to eradicate biofilm produced by Pseudomonas aeruginosa.

Otitis externa (OE) is one of the most frequently diagnosed dermatological diseases in dogs, having a multifactorial aetiology. Among the bacterial agents associated with canine OE, Pseudomonas aeruginosa is of special concern owing to its frequent multidrug resistance profile and ability to form biofilms related to the infection's chronicity and recurrence. The main objective of this study was to evaluate and compare the antibiofilm activity of two innovative antimicrobials-an otological gel containing a synthetic antimicrobial peptide and Lavandula angustifolia essential oil-with gentamicin (a conventional antibiotic) using biofilm-producing P. aeruginosa isolates obtained from dogs with OE. The biofilm eradication capacity of gentamicin, otological gel and lavender oil was determined against a collection of 12 P. aeruginosa biofilm-producers among 35 clinical isolates obtained from the ear canals of dogs with OE. Also, the antimicrobial activity of the otological gel against P. aeruginosa biofilms was assessed in an invitro model of dog cerumen. Lavender oil showed the best effectiveness after 30 min of contact, eradicating 58.3% (seven of 12) of the isolates, and gentamicin showed full eradication (12 of 12) after 24 h. The otological gel acted more slowly than the lavender oil; yet at 24 h, the antibiofilm capacity of both compounds was similar, with no significant difference between them. It also was found that triglycerides from synthetic cerumen earwax had antipseudomonal activity and, when combined with the otological gel, led to the full eradication of P. aeruginosa. The results of this invitro study indicate that lavender oil and the otological gel may be effective topical treatments for canine OE promoted by P. aeruginosa biofilm-producers, as alternatives to gentamicin.

Strategical Ways for Dental Anxiety Management Prior to Third Molar Extraction.

A patient experiencing moderate to severe anxiety often skips regular check-ups, choosing to visit only when necessary. As a result, their oral health worsens. Various variables can affect anxiety such as age, gender, culture, and previous experiences. One of the most frequent oral surgical procedures is extracting the third molar, which is accompanied by severe pain, tension, and anxiety, the use of invasive equipment, and local anesthetic. A combined effect was found of four factors, including the patient, dental employees, setting, and dental procedure, which can trigger and affect dental anxiety. Both pharmacological and non-pharmacological approaches were explored; as a non-drug approach, anxiety was managed by music therapy. Acupuncture, music, audio-visual aids, and lavender oil showed an effect that helped in reducing anxiety. Patients' dental anxiety can be reduced through behavioral management such as educating the patients before the surgical procedure, relaxation strategies, biofeedback, and exposure therapy. Medicationsinclude benzodiazepines, nitrous oxide (N2O), and midazolam. Effective treatment relies on collaboration between the patients anddentists; calm patients not only provide a manageable environment for the dental team but can also result in better treatment outcomes.

AROMA THERAPY INTERVENTION TO IMPROVING SLEEP QUALITY IN PATIENTS WITH CARDIOVASCULAR PROBLEMS

Background: Poor sleep quality will lead to stressful conditions which can cause the release of epinephrine and norepinephrine has the effect of increasing the oxygen demand for the myocardium. Aromatherapy is recommended as a no-side-effect, non-invasive, inexpensive and painless method to reduce anxiety and pain. improve sleep quality of cardiovascular patients. Purpose: The aim of this study is to analyze the potential of aromatherapy interventions in patients with cardiovascular disorders. Methods: The research method in this article uses a literature study approach. The articles will be selected based on the following criteria. The search sources in this article use scientific-based databases (ScienceDirect, PubMed, EBSCOhost, and Wiley Library Online). The search starts from December 2022 using the keywords Cardiac Patients – Aromatherapy – Sleep quality. Result: Inhalation aromatherapy with peppermint or lavender essential oil can improve the sleep quality of cardiovascular patients. Inhaling aromatic oil stimulates the sense of smell affecting the central nervous system. Lavender oil can reduce fatigue, anxiety, heart rate, respiratory rate, and blood pressure, improve lung and brain oxygenation, and improve sleep quality. The use of essential oils can be a safe and cost-effective therapy for sleep disorders and can reduce the overuse of prescription medications. Implication: Lavender and peppermint essential oils have a therapeutic effect that relaxes the body. These effects can make the body feel relaxed and release hormones that can help patients sleep. However, this intervention still needs further research specifically from the aspect of dose and medium of aromatherapy.

Evaluation of the inhalation of lavender oil on physiological and psychological effects: A pilot study

Evaluation of the inhalation of lavender oil on physiological and psychological effects: A pilot study

The Effect of Giving Red Ginger Oil On Pain After Iud Installation at Midwifery Independent Practice in Turen Malang Regency

ABSTRACT IUD installation pain is caused by a foreign body that enters the uterus, so to help the adaptation process, it is necessary to have treatment both pharmacologically and non-pharmacologically. The more frequent the pain that arises, the more recommended non-pharmacological treatment because it has a low risk. In post-IUD pain, there is complementary care in the form of essential oils. Essential oils often used are olive oil, peppermint oil, lavender oil, coconut oil, clove oil, and the latest red ginger oil. Complementary care can be done by giving Red Ginger Oil as a natural analgesic. The purpose of this study is to examine the impact of administering red ginger oil on post-IUD discomfort in a midwifery practice in Turen, Malang Regency. Experimental quantitative research using a one-group pre- and post-test design is what this study employs. The 18 people that filled out the survey were chosen using a quota system. The findings of the research were obtained three days after the controlled intervention, after the administration of Red Ginger Oil. Supplemental care, which posed less of a threat, was easily comprehended by patients. This research found that red ginger oil greatly alleviates uterine discomfort when administered topically. Keywords: IUD, Red Ginger Oil, Pain

The Therapeutic Effect of Shirvan Herbal Ointment on Human Cutaneous Leishmaniasis Compared with Glucantime.

Cutaneous leishmaniasis (CL) infection is caused by the Leishmania major (L. major) parasite and affects 1.5 to 2 million people worldwide each year. Although research into vaccines and antiparasitic drugs has been somewhat successful, their adverse effects include high toxicity, prolonged regeneration, and scarring. This has highlighted the importance of research to replace natural products with antibacterial and antioxidant properties, such as vegetable extracts and oils. Since, the anti-leishmaniasis effect of each of the components of Shirvan herbal ointment (aloe vera, Brazembel, Nigella sativa, propolis, lavender, and olive oil) has been separately studied and confirmed, it seems that the combination of these components can have an increasing anti-leishmanial effect to treat CL. Therefore, this study investigated the therapeutic impact of Shirvan herbal ointment on Iranian patients with leishmaniasis in comparison with glucantime (meglumine). Sixty patients with leishmaniasis were divided into the control and test groups. The control and test groups received intralesional glucantime and Shirvan herbal cream (two times daily), respectively. The size mean of the lesion was 51.5 ± 32.5 before and 11.11 ± 16.28 after treatment in the control group and 50.8 ± 31.2 before and 0.0 ± 0.0 after treatment in the test group. In addition, the period mean of treatment was 43.9 ± 14.4 days and 30.5 ± 7.4 days in the control and test groups, respectively. There was a significant difference in lesion size between the two groups after treatment. Data suggested that Shirvan herbal ointment can be an alternative drug in the treatment of human CL.

Perbedaan Pengaruh Jahe, Aromaterapi Oil Lavender dan Musik Klasik terhadap Pengurangan Emesis Gravidarum pada Ibu Hamil Trimester I

Emesis gravidarum is the most common disorder found in 70% of pregnant women. Nonpharmacological treatments that can be used are ginger, oil lavender aromatherapy, and classical music. The purpose of this study was to determine the differences in the effect of ginger, oil lavender aromatherapy, and classical music on reducing emesis gravidarum in the first trimester of pregnant women. The research method used a quasy-experiment with a three-group comparison pretest-posttest design in a time series. The sampling technique used purposive sampling, the sample size was 28 pregnant women in the working area of the Jogonalan II Health Center, 22 pregnant women in the Kebonarum Health Center, and 25 pregnant women in the Prambanan Health Center. Then divided into 3 groups, namely the ginger group, the oil lavender aromatherapy group, and the classical music group. Ginger reduced the emesis gravidarum score by 3,23. Oil lavender aromatherapy reduced the emesis gravidarum score by 4,42. Classical music lowers the emesis gravidarum score by 2,03. This study shows that there is an effect of reducing emesis gravidarum in first trimester pregnant women, but the difference in the effects is not significant because they have the same p value = 0,000 <0,05.ginger, oil lavender aromatherapy, classical music, emesis gravidarum

Green method for quantification of lavender and sweet orange essential oils in blends by synchronous fluorescence first derivative

A simple green method for the quantification of sweet orange and lavender essential oils (EOs) content in blends was developed using synchronous fluorescence of the first derivative spectra and sample preparation by surfactant-free microemulsions (SFMEs). Excitation and emission wavelengths pairs (λex/λem) were determined for both EOs (at 336/436 nm for sweet orange and at 330/388 nm for lavender). Optimization was conducted to establish the condition to prepare the SFMEs systems: 50 μL of oily phase containing EO diluted in octan-1-ol (1:4, v/v), 2.0 mL of water and propan-1-ol up to 5.0 mL final volume. Different proportions of EO in the oily phase were evaluated for analytical curves construction. Synchronous fluorescence measurements were performed and the acquired data were corrected by inner filter effect. Considering the first derivative of the synchronous scanning, data were extracted at 316.8 nm (Δλ = 100 nm) and 352.0 nm (Δλ = 58 nm) for sweet orange and lavender EOs, respectively, with correlation coefficients above 0.99. Limits of quantification of 7.97 μg mL−1 and 65.80 μg mL−1 were obtained indicating that 0.46% of sweet orange and 3.69% of lavender EOs can be quantified in mixtures. Simulated blends (25:75%, 50:50% and 75:25%, volume proportions) were also evaluated with recoveries of 71.5 – 128.9% and coefficient of variation between 3.1% – 11.6%. Additionally, the method's greenness was evaluated through three green metrics (Analytical Eco-Scale, GAPI, and AGREE) with consistent results.

Outcomes of Aromatherapy in Nausea and Vomiting After Total Knee Arthroplasty

PurposeThe purpose of this study is to assess the effectiveness of aromatherapy for postoperative nausea and vomiting (PONV) after total knee arthroplasty (TKA) under spinal anesthesia. DesignProspective randomized four-arm placebo-controlled trials MethodsOne hundred and twenty subjects were allocated to each of the four groups based on the application of aromatic oil in subjects manifesting PONV: group 1 (lavender), group 2 (lemon), group 3 (peppermint), and group 4 (normal saline placebo). Aromatherapy was administered to all subjects immediately after surgery. Antiemetics were provided to subjects with significant nausea or vomiting. The severity of nausea and vomiting in subjects post-TKA was evaluated using the Halpin nausea and vomiting scale (HNV). The HNV and the concentration of antiemetic drug use were evaluated. Subjects’ satisfaction with treatment for PONV was evaluated at discharge. FindingsHNV scores did not differ significantly between groups immediately after surgery until the third postoperative day (P > .05). The amount of antiemetic drug used in group 3 was significantly lower among the groups (P = .030). The subject satisfaction scale did not differ significantly among groups (P = .837). ConclusionsAromatherapy using peppermint oil reduced the amount of antiemetics used to treat PONV after TKA under spinal anesthesia with comparable subject satisfaction. Lavender and lemon oils did not reduce the use of antiemetics after TKA.

Effect of inhaled aromatherapy on sleep quality in critically ill patients: A systematic review and network meta-analysis.

This study aimed to compare the effect of inhaled aromatherapy using various essential oils on the sleep quality of critically ill patients. Inhalation of essential oils significantly promotes the physiological and psychological health of patients in intensive care units (ICUs). However, research identifying and ranking the effects of different essential oils on the sleep quality of critically ill patients is lacking. This study followed the PRISMA Extension Statement for Reporting of Systematic Reviews Incorporating Network Meta-Analyses of Health Care Interventions (PRISMA-NMA) guidelines. A comprehensive search of five databases (Embase, MEDLINE, the Cochrane Library, CINAHL and PsycINFO) was conducted from their inception to March 15, 2023 (with an additional eligible study included dated August 14, 2023). Google Scholar was used as a supplementary method. Frequentist NMA was used to determine the effects of various essential oils. Certainty of evidence (CoE) was assessed using Confidence in Network Meta-Analysis (CINeMA). A total of 11 trials involving 690 critically ill patients were included in the analysis. The NMA of inhaled aromatherapy revealed that the combination of lavender, Matricaria recutita, and neroli essential oils (ratio 6:2:0.5) resulted in the most significant improvement in sleep quality compared to usual care, followed by Rosa damascene, peppermint, Citrus aurantium, pure sunflower oil and lavender oil alone. The overall CoE for the results was rated as low. The results of this study indicate that a combination of lavender, Matricaria recutita and neroli essential oils significantly positively affected sleep quality among critically ill patients. Despite the low quality of evidence, inhaled aromatherapy is non-invasive and easy to use. Inhaled aromatherapy can effectively improve sleep quality among critically ill patients. No patient or public contribution applies to this work. The study protocol was registered to the PROSPERO International Prospective Register of Systematic Reviews (protocol number CRD42023433194).

Enhancing the durability of mosquito repellent textiles through microencapsulation of lavender oil

Enhancing the durability of mosquito repellent textiles through microencapsulation of lavender oil

Effects of Aromatherapy on Physical and Psychological Symptoms in Cancer Patients: A Systematic Review and Meta-analysis.

Numerous physical and psychological symptoms experienced by cancer patients seriously affect their normal lives. Many academics and medical professionals have attempted to use aromatherapy in this situation to help cancer patients manage their physical and emotional problems. To systematically investigate the efficacy of aromatherapy on physical and psychological symptoms in cancer patients. A systematic review and meta-analysis of randomized controlled trials was performed. Four electronic databases were searched. The review process followed a registered priori review protocol and was reported using Preferred Reporting Items for Systematic Reviews and Meta-analysis guidelines. Data extraction and quality assessment were performed in parallel. Twenty-six studies with 2912 subjects were included. Meta-analysis showed that aromatherapy significantly improved sleep quality, fatigue, anxiety, and depression. We performed a subgroup analysis according to the different plant or animal aromatics contained in the oil, which found that lavender oil significantly reduced preoperative anxiety. In addition, aromatherapy massage was superior to inhaled aromatherapy in reducing anxiety. Moreover, cancer patients who used aromatherapy reduced the frequency of vomiting in 24 hours. Aromatherapy is a useful treatment for improving sleep quality and reducing symptoms of fatigue, anxiety, and depression in cancer patients, as well as the frequency of vomiting over 24 hours. Healthcare providers can use aromatherapy to alleviate psychological and physical symptoms in cancer patients. The use of lavender oil and massage is recommended in clinical settings to improve anxiety symptoms in cancer patients.

Effect of Lavender Oil on Social Anxiety Among First-Year College Students

ABSTRACT Background: In order to build learning environments that support both academic and psychological growth, it is important to recognize and manage social anxiety, a problem that is frequently experienced by first-year students in educational settings. Objective: The aim of this study is to assess effectiveness of lavender oil on social anxiety among first-year college students. Methods: A quasi-experimental research design was employed. The 100 samples selected using the non-probability purposive selection strategy. The instrument has two sections: demographic information and the Social Interaction Anxiety Scale. Results: The results reveal that in the pretest, 70 (70%) had no anxiety, 12 (12%) had mild anxiety, and 18 (18%) had moderate anxiety, while in the post-test, 85 (85%) had no anxiety, 13 (13%) had mild anxiety, and 2 (2%) had moderate anxiety among first-year students. Students had a mean anxiety score of 37.90 ± 8.59 before and 21.53 ± 5.79 after the test. The mean difference score was 16.37, while the mean difference percentage was 41%. The estimated paired “t”-test result of t = 16.209 was found to be statistically significant at P < 0.001. This demonstrates that the lavender oil on social anxiety administered to students was effective in lowering anxiety levels during the post-test. Conclusion: Lavender oil is a promising natural treatment for social anxiety, providing a simple and accessible option with little side effects.

Evaluation of lavender and rose aromatherapies on the success of inferior alveolar nerve block in symptomatic irreversible pulpitis: A randomized clinical trial

Trial designThis is a prospective, block-randomized, blinded, multiple arm and parallel-group superiority clinical trial. MethodsSeventy-eight patients satisfying the recruitment standards, were randomly allocated into three groups as follows: Group I (n = 26) – Inferior alveolar nerve block (IANB) devoid of aromatherapy (AT); Group II (n = 26) - IANB with lavender AT and Group III - IANB with rose AT (n = 26) with the help of the ultrasonic aroma diffuser (with respective oils)for 20 min/2 h in operatories 1,2 and 3 respectively. For AT, 3–4 drops of lavender and rose-conditioned oils were added from a 100 ml solution containing 100 mg of these medicinal plants. The pre-operative (PRO) and access opening (AO) pain as well as the anxiety of patients were recorded using the Visual Analog Scale (VAS) and Modified Dental Anxiety Scale (MDAS) respectively. Data thus obtained was entered into the Excel sheet and subjected to statistical tests (analysis of variance and paired t-test). The p-value less than 0.05 was considered statistically significant. ResultsGroup I showed non-significant disparity between PRO and AO for both VAS as well as MDAS (p = 0.62, p = 0.71). However, group II (p = 0.04, p = 0.02) and group III (p = 0.03, p = 0.01) revealed significant differences between PO - AO VAS and MDAS. MDAS and VAS intergroup comparison revealed a significant difference among groups I and II (p = 0.03, p = 0.04), and groups I and III (p = 0.02, p = 0.03). However non-significant disparity was observed among groups II and III (p = 0.85, 0.34). Moreover, there was a statistically significant reduction in anxiety levels in females compared to males after rose AT (p = 0.02). Nevertheless, groups I and II did not show any gender predilection for anxiety as well as pain. ConclusionAlleviation of dental anxiety as well as reduction in pain during AO of teeth with SIP can be achieved using Lavender and rose AT. In female patients, rose AT can be preferred over lavender AT.

Study on biological activity of Lavandula angustifolia and Citrus aurantiifolia essential oils

Abstract The essential oils of Lavandula angustifolia (lavender) and Citrus aurantiifolia (lime), have been used in several applications such as food industry, pharmacy, and cosmetics industries. In this report, we examined the biological activities of lavender and lime essential oils through several studies using β-carotene bleaching assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, antidiabetic assay, antibacterial assay, and also measure its total phenolic content (TPC). The functional group of lavender and lime was studied using Fourier transform infrared spectroscopy (FTIR), while the volatile compound was evaluated using Gas chromatography–mass spectrometry (GC-MS) analysis. The result showed that in DPPH assay, both of lavender and lime essentials oil have low activity with IC 50 of 1966.02 ppm and 4303.42 ppm, respectively, while in beta-carotene bleaching assay, lime has higher antioxidant activity of 39.03% compared to lavender as of 17.55%. Antibacterial activity against S. aureus showed that both lavender and lime have good activity as of 18.13 mm and 18.06 mm, respectively. The antidiabetic activity using α-glucosidase inhibitory activity presented that lavender and lime had high activity of 89.58 % and 88.30 %, respectively. Furthermore, the total phenolic content of lavender was slightly higher as of 480.00 mg GAE/g compared to lime as of 393.33 mg GAE/g. The GC-MS analysis of essential oils showed that lavender contains linalyl acetate (38.78%), linalool (36.12%), and 1,8-cineole (8.38%), while lime contains d-limonene (73.72%), beta-pinene (9.83%) and gamma-terpinene (8.24%), respectively. The present study showed the potential use of lavender and lime essential oils for antidiabetic, antimicrobial, and antioxidant agents.

Benefits of Lavender Aromatherapy in Reducing Menstruation Pain in Teenage Women

Adolescence is a transition stage that causes physical, emotional, and psychological changes. A disorder that often occurs in young women is menstrual pain which can change a woman's emotions in a short time, disrupting her routine and learning process. Aromatherapy can be applied directly through direct massage on the skin using plant extracts. Aromatherapy can treat respiratory problems, and pain, and reduce stress levels. Lavender oil is rich in analgesic essentials which can stimulate the nervous system to bring a feeling of calm to pain sufferers. The main aim of the research was to see how to reduce pain intensity in teenagers before and after being given lavender aromatherapy. The objective of implementing this PkM activity is so that young women at SMA Negeri 1 Perbaungan can experience increased knowledge and be able to carry out appropriate treatment for menstrual pain through the use of lavender aromatherapy. PkM activities were conducted through socialization, where the participants were young women at SMA Negeri 1 Perbaungan, totaling 30 participants. The PkM results show that many young women's menstrual pain conditions change from severe pain to mild and moderate pain after being given lavender aromatherapy therapy. This proves that giving lavender aromatherapy can reduce menstrual pain in young women. Apart from that, the socialization of PkM has been proven to be able to increase the knowledge of young women in understanding and applying it when menstrual pain occurs.

(091) EXPOSURE OF THE VESTIBULE TO A POSSIBLE ALLERGIC TRIGGER AT A VERY YOUNG AGE: LIFELONG NEUROPROLIFERATIVE VESTIBULODYNIA

Abstract Introduction Vestibular fibroblasts from patients with neuroproliferative vestibulodynia (NPV) versus age-matched controls produced higher proinflammatory cytokine levels of various interleukins when provoked with candida albicans in vitro. In genetically susceptible patients, this enhanced cytokine synthesis is hypothesized, in part, to lead to neuroproliferation in vestibular tissue resulting in allodynia/hyperalgesia. Patients with acquired NPV, who initially experienced pain-free penetration in their early adult reproductive life, may experience persistent severe vestibular burning following an allergic reaction to a topical agent applied to the non-keratinized vestibular epithelium. In contrast, patients with lifelong NPV present with the classic history of inability to insert a tampon at menarche (mean age 12.4 years). Patients with lifelong NPV may have unknowingly been exposed to an allergen early in their childhood, potentially even triggered from ointments used in infancy to protect from diaper rash, resulting in local vestibular mast cell accumulation in genetically susceptible patients. Agents commonly used to prevent are zinc oxide 40%, (Desitin) with inactive ingredients petrolatum, cod liver oil, lanolin, talc, glycerin, sorbitan sesquioleate, beeswax, tocopheryl acetate and fragrance and A+D ointment that includes petroleum jelly, lanolin, lavender oil, light liquid paraffin, vitamin A and vitamin D. It is possible an allergic reaction could result from any of these ingredients when applied to the non-keratinized vestibular epithelium. Objective We wished to better understand the possible triggering event in patients with lifelong NPV. Methods A chart review was performed of patients who presented between January 2019 and June 2023 who were clinically suspected to have lifelong NPV based on a history of inability to use tampons and inability to have pain-free penetration and histopathlogically confirmed to have NPV. Information was obtained from a parent or caregiver who regularly changed patient’s diapers as to what agents were utilized to manage diaper rash. Results Our patient cohort included 14 individuals with vulvas, mean age 25 (range 18-37) years. One patient prone to diaper rash was treated with A+D baby diaper rash ointment and Flanders buttocks ointment (zinc oxide, white petrolatum, white beeswax, Peruvian balsam). One patient had a “bad” yeast infection as a baby and received “topical antibiotics”. Five patients claimed Desitin and Vaseline were used while six patients claimed Desitin alone, two of whom cryied every time they Desitin was applied. One patient from Puerto Rico, received “a sock filled with cornstarch that dabbed the vaginal area and crevices after wiping the area clean”. Conclusions The endodermal vestibular epithelium is not protected by a keratin layer, including in babies at a very young age. In genetically susceptible babies, an allergic reaction to an active/inactive agent used to prevent or treat diaper rash could have potentially triggered mast cell accumulation in the baby’s vestibule leading to increased synthesis of cytokines and growth factors resulting in neuroproliferation consistent with lifelong NPV, evidenced by the inability to place a tampon at menarche. More research into this concern is needed. Disclosure No.

Anthelmintic effects of some medicinal plants on different life stages of Fasciola hepatica: Evidence on oxidative stress biomarkers, and DNA damage.

Fasciolosis caused by Fasciola hepatica is a major public health and economic problem worldwide. Due to the lack of a successful vaccine and emerging resistance to the drug triclabendazole, alternative phytotherapeutic approaches are being investigated. This study investigated the in vitro anthelmintic activity of Lavender (Lavandula angustifolia) and carob (Ceratonia siliqua L.) essential oils (EOs) against F. hepatica. The in vitro study was based on an egg hatch assay (EHA), adult motility inhibition assays, DNA damage, reactive oxygen species (ROS) level along with several oxidative stress biomarkers including glutathione peroxidase (GSH), and glutathione-S-transferase (GST), superoxide dismutase (SOD) and malondialdehyde (MDA). To this end, different concentrations of L. angustifolia and C. siliqua EOs (1, 5, 10, 25 and 50 mg/mL) were used to assess anthelmintic effects on different life stages including egg, and adults of F. hepatica for 24 hrs. The results indicated that these EOs play a significant role as anthelminthics, and the effect was dependent on time and concentration. The in vitro treatment of F. hepatica worms with both L. angustifolia and C. siliqua EOs increased DNA damage, ROS production and induction of oxidative stress (decreased SOD, GST and GSH, and increased MDA), significantly compared to control. Therefore, it can be concluded that L. angustifolia and C. siliqua EOs have the potential to be used as novel agents for the control and treatment of F. hepatica infections. Further studies are required to investigate their pharmacological potential and effectiveness in vivo for the treatment of parasitic infections.

Formulation and Evaluation of Calendula and Lavender Oil Based Topical Antifungal Gel

Aim: Calendula officinalis and Lavendula angustifolia are traditional medicinal plants that have antifungal activity. A combination of these two plants has not been known for its activity against fungus. The present study deals with the formulation and evaluation of herbal topical Antifungal gel containing essential oils of both plants. Study Design: Proto type research design. Place and Duration of Study: Smt. R. D. Gardi Pharmacy College, Nyara- Rajkot. January 2024 to April 2024. Methodology: As 90% of skin infections are caused by Candida albicans, the antifungal activity of topical gel against this species was assessed. In this investigation, we created an antifungal herbal gel by combining carbopol 940 and other excipients with the essential oils of Calendula officinalis and Lavendula angustifolia in varying concentrations. For compatibility study, FTIR study would be done. Evaluation of Antifungal gel performed by using different tests likes, pH, Stability, Extrudability, Spreadability, Viscosity, Antifungal activity against Candida albicance, and in-vitro drug diffusion study. Results: As a result, the formulation's physiochemical characteristics, in vitro antifungal efficacy, and stability analysis (stable even after 30 days) were assessed. Every herbal gel formulation with a pH of 5–7 had favorable outcomes for physiochemical measures. Out of all the formulations, batch no 4 exhibited superior release characteristics (98 %) and zone of inhibition in comparison to the other formulated batches. The drug release profile of batch 4 showed great results. Conclusion: This is the first study on the scientific evaluation of Calendula officinalis and Lavendula angustifolia essential oil as a gel for antifungal activity. Thus this study reveals both good antifungals and in-vitro drug release; their essential oil may be formulated as Topical Gel Antifungal with satisfactory physicochemical parameters.

Sex differences in the effects of aromatherapy on anxiety and salivary oxytocin levels.

Aromatherapy is a holistic healing method to promote health and well-being by using natural plant extracts. However, its precise mechanism of action and influence on the endocrine system remains unclear. Since recent studies reported that a neuropeptide, oxytocin, can attenuate anxiety, we hypothesized that if oxytocin secretion is promoted through aromatherapy, it may improve mood and anxiety. The present study is aimed to investigate the relationship between oxytocin and the effects of aromatherapy with lavender oil on anxiety level, by measuring salivary oxytocin levels in healthy men and women. We conducted a randomized open crossover trial in 15 men and 10 women. Each participant received a placebo intervention (control group) and aromatherapy with lavender oil (aromatherapy group). For the aromatherapy group, each participant spent a 30-min session in a room with diffused lavender essential oil, followed by a 10-min hand massage using a carrier oil containing lavender oil. Anxiety was assessed using the State-Trait Anxiety Inventory (STAI) before the intervention, 30-min after the start of intervention, and after hand massage, in both groups. Saliva samples were collected at the same time points of the STAI. In women, either aromatherapy or hand massage was associated with a reduction in anxiety levels, independently. Moreover, salivary oxytocin levels were increased after aromatherapy. On the other hand, in men, anxiety levels were decreased after aromatherapy, as well as after hand massage, regardless of the use of lavender oil. However, there were no significant differences in changes of salivary oxytocin levels between the control and aromatherapy groups during the intervention period. Interestingly, there was a positive correlation between anxiety levels and salivary oxytocin levels before the intervention, but a negative correlation was observed after hand massage with lavender oil. The results of the present study indicate that in women, aromatherapy with lavender oil attenuated anxiety with increase in oxytocin level in women, whereas in men, there was no clear relationship of aromatherapy with anxiety or oxytocin levels but, there was a change in correlation between anxiety and oxytocin. The results of the present study suggest that the effect of aromatherapy can vary depending on sex.