Lavandula Angustifolia Essential Oil Research Articles

Programming and validation of a novel green procedure for the production of essential oil from Lavandula angustifolia

Lavandula angustifolia (lavender) essential oil (LEO) is extremely popular and beneficial. In the present study, LEO was extracted with green ultrasound-assisted supercritical CO2 (UscCO2) and other classical methodologies. The highest yield of LEO (5.32 % (w/w)) was achieved with the UscCO2 process, followed by hexane extraction (4.32%), scCO2 extraction (3.94 %) and hydrodistillation (HD; 3.69 %). The contents of two dominant aromatic ingredients, linalool (29.93 %) and linalyl acetate (35.08 %), obtained by the UscCO2 procedure were also the highest. Remarkably, the linalyl acetate content in UscCO2-derived LEO was nearly triple that in LEO extracted via HD (12.33 %). The novel UscCO2 technique not only provided the highest LEO quality but also revealed the highest extraction efficiency under mild extraction parameters. The dynamic solubility of LEO in the UscCO2 and scCO2 procedures was further determined to confirm the kinetic efficacy. The dynamic solubility was simulated with three density-based models, which provided excellent agreement with the experimental results. The approximate heat of vaporization, total heat of solution and heat of solvation obtained from UscCO2 dynamic extraction of LEO were significantly lower than those obtained from the scCO2 procedure. The kinetic and thermal effects of ultrasonic energy substantially contributed to the excellent performance of UscCO2.

Modulating the growth, immune/antioxidant traits, immune-related genes, and resistance to Aeromonas veronii infection in Oreochromis niloticus reared under high stocking densities by dietary Lavandula angustifolia essential oil

Modulating the growth, immune/antioxidant traits, immune-related genes, and resistance to Aeromonas veronii infection in Oreochromis niloticus reared under high stocking densities by dietary Lavandula angustifolia essential oil

The efficiency of Thymus vulgaris, Lavandula angustifolia, and Cupressus sempervirens essential oils in suppression of Hymenoscyphus fraxineus on Fraxinus angustifolia seedlings

AbstractDue to intense ash dieback, this research tested the bioactivity of Thymus vulgaris, Lavandula angustifolia, and Cupressus sempervirens essential oils (EO) against Hymenoscyphus fraxineus. In vitro testing showed that EOs had a pronounced fungitoxic effect on H. fraxineus. The most effective EO was extracted from T. vulgaris, while EOs obtained from L. angustifolia and C. sempervirens were less effective and showed the same effect. At a concentration of 1%, all tested EOs inhibited mycelial growth, while 0.01% and 0.1% concentrations of EO extracted from T. vulgaris also inhibited the growth of H. fraxineus. The in planta test of EO extracted from T. vulgaris conducted on three‐year‐old Fraxinus angustifolia seedlings showed a reduction in the size of necrotic lesions and a better crown condition compared to untreated seedlings. This research demonstrates, for the first time, the efficiency of EOs in suppressing ash dieback making a path for the development of new biological protection measures against H. fraxineus.

Assessment of lavender (Lavandula angustifolia) essential oil as a natural anesthetic and sodium bicarbonate as a sedative on physiological and histopathological status of Tilapia zilli during the transport practices

Assessment of lavender (Lavandula angustifolia) essential oil as a natural anesthetic and sodium bicarbonate as a sedative on physiological and histopathological status of Tilapia zilli during the transport practices

The Local Anesthetic Activity of Lavandula angustifolia and Eugenia caryophyllata Essential Oils

Previous studies show that Lavandula angustifolia (lavender) and Eugenia caryophyllata (clove) essential oils can help relieve dental pain. Clove oil and lavender oil were tested as topical anesthetics in this study on rabbits, and their effects and likely mechanisms of action were analyzed. Clove oil and lavender oil were extracted by hydrodistillation using a Clevenger-setup apparatus. Topically applying lidocaine, clove oil, or lavender oil topically all significantly reduced corneal sensitivity. The sensitivity of the cornea was successfully reduced by treatments consisting of lidocaine at a concentration of 0.5%, 25 μg of clove oil, and 50 μg of lavender oil. When clove oil is applied topically to the cornea, it produces effects similar to those of a local anesthetic due to the involvement of the cholinergic system. To achieve the desired effect of producing local anesthesia in the cornea of the rabbit, lidocaine, clove oil, and lavender oil were applied topically to the animal. A noticeable local
 anesthetic effect was produced when sub-anesthetic doses of lidocaine were combined with sub-anesthetic doses of lavender or clove oil.

Authentication of Lavandula angustifolia Mill. (Lamiaceae) essential oil using physical property, gas chromatography, enantiomeric selectivity, and stable isotope analyses

ABSTRACT Authentic Lavandula angustifolia (lavender) essential oil samples (n = 41) were procured from six producing countries. Essential oils were analyzed to determine physical properties and to determine profiles and related data by GC/MS, GC/FID, enantioselective GC, and GC/IRMS. The current study identified 43 volatile compounds that were detected in all authentic lavender samples (n = 41), and which can be considered authentic markers. Enantiomeric ranges for 15 volatile compounds and stable isotope ranges for four prominent compounds were determined in authentic lavender. Authentic samples and associated data were used to assess the quality of commercially available lavender essential oil samples (n = 12). 75% of the commercial samples studied were adulterated,and 17 volatile compounds were detected in these samples which can be considered markers for adulteration. This study establishes the utility and importance of using a multifaceted analytical approach to differentiate quality and determine authenticity of lavender essential oil.

Nanoemulsion of Lavandula angustifolia Essential Oil/Gold Nanoparticles: Antibacterial Effect against Multidrug-Resistant Wound-Causing Bacteria.

Hospitalized patients are severely impacted by delayed wound healing. Recently, there has been a growing focus on enhancing wound healing using suitable dressings. Lavandula angustifolia essential oil (LEO) showed potential antibacterial, anti-inflammatory, and wound healing properties. However, the prepared gold nanoparticles possessed multifunctional properties. Consequently, the present investigation aimed to synthesize a novel nanosystem consisting of nano-Lavandula angustifolia essential oil and gold nanoparticles prepared through ultrasonic nanoemulsifying techniques in order to promote wound healing and combat bacterial infection. LEO showed potent antibacterial activity against Klebsiella pneumoniae, MRSA and Staphylococcus aureus with minimum inhibitory concentration (MIC) values of 32, 16 and 16 µg/mL, respectively, while exhibiting low activity against Proteus mirabilis. Interestingly, the newly formulated nano-gold/nano-Lavandula angustifolia penetrated the preformed P. mirabilis biofilm with a full eradication of the microbial cells, with MIC and MBEC (minimal biofilm eradication concentration) values reaching 8 and 16 µg/mL, respectively. The cytotoxic effect of the novel nanoformula was also assessed against WI-38 fibroblasts vero (normal) cells (IC50 = 0.089 mg/mL) while nano-gold and nano-Lavandula angustifolia showed higher results (IC50 = 0.529, and 0.209 mg/mL, respectively). Nano-gold/nano-Lavandula angustifolia formula possessed a powerful wound healing efficacy with a 96.78% wound closure. These findings revealed that nano-gold/nano-Lavandula angustifolia nanoemulsion can inhibit bacterial growth and accelerate the wound healing rate.

Kokedama and essential oils had a relaxing psychophysiological effect on Taiwanese women during the COVID-19 pandemic

BackgroundDuring the COVID-19 pandemic, we designed an indoor nature activity program for citizens with a relaxing effect similar to forest bathing to promote their physical and mental health. We integrated an indoor horticultural activity (Kokedama) with the breathing of Pseudotsuga menziesii (P. menziesii) and Lavandula angustifolia (L. angustifolia) essential oils (EOs) with the goal of creating a nature-inspired environment in an indoor setting where participants would feel as if immersed in a forest atmosphere. MethodsTaiwanese women participated in the experiment, using two Saturday mornings, one Saturday in a university classroom in the city center; and the other Saturday in a workshop in a Suburban Park. Intra-group comparisons were used to assess the Physiological responses to urban and suburban environmental stimuli and measured self-reported psychological responses. We recorded parameters associated with heart-rate variability and brainwaves. We also administered the State-Trait Anxiety Inventory-State (STAI-S) questionnaire before and after the participants had completed the entire program. ResultsAfter the participants had breathed the P. menziesii and L. angustifolia EOs, the levels of some physiological parameters increased (standard deviation of normal-to-normal intervals, normalized high frequency, and high alpha wave) and those of others decreased (normalized low frequency, low- to high-frequency ratio power, high beta wave, and gamma wave). These findings were corroborated by the data from the STAI-S questionnaires. ConclusionsThe psychophysiological data from this study provide significant scientific evidence for the health benefits of an indoor nature activity program in women.

Development and Characterization of an Active Transparent Biodegradable Tara Gum Film Incorporated with Lavandula angustifolia Essential Oil

Development and Characterization of an Active Transparent Biodegradable Tara Gum Film Incorporated with Lavandula angustifolia Essential Oil

Biological activities and chemical characterization of Lavandula angustifolia essential oil from Seraïdi, Northeastern Algeria

Abstract. Hadj Moussa AH, Benaliouche F, Sbartai I, Sbartai H. 2023. Biological activities and chemical characterization of Lavandula angustifolia essential oil from Seraïdi, Northeastern Algeria. Biodiversitas 24: 4535-4543. The objectives of this study are to examine the chemical composition of essential oil (EO) extracted by hydrodistillation from the flowering tops of dry lavender (Lavandula angustifolia) harvested in the town of Seraïdi in Northeastern Algeria and evaluate its antifungal, antibacterial, and antioxidant potential. The chemical analysis of this essential oil was carried out by gas chromatography coupled with mass spectrophotometry (GC-MS). The antimicrobial activity was evaluated by dilution method in solid and liquid medium, and the antioxidant activity was carried out using the DPPH° radical scavenging assay. It revealed the presence of 20 compounds representing the general component of this oil with a yield of 1.71%. This oil is composed mainly of Linalool (31.27%), followed by Camphor (16.21%), Linalool Oxide (11.98%), and Linalyl Acetate (11.93%). Other constituents were identified at relatively medium [2-Furarmethanol (7.49%), 1,8-Cineole/eucalyptol (6.76%), Borneol (5.34%)] and low contents [1-Hexyl butyrate (1.25%)]. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of lavender EO against Fusarium roseum were 3000 µg/mL and 4000 µg/mL, respectively. Lavender OE had moderate antibacterial activity against Pseudomonas savastanoi. The antioxidant activity of lavender EO was lower than that of vitamin C.

Antibacterial and antifungal activity of Lavandula angustifolia and Thymus vulgaris essential oils grown in the Algerian west coast

ABSTRACT The screening of natural products in the quest for new antimicrobial and antifungal agents has become the need of the hour in order to combat drug-resistant pathogenic microbes.The aim was to evaluate the antibacterial and antifungal activities of Lavandula angustifolia and Thymus vulgaris essential oils (EOs).The antimicrobial activity was tested on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus using agar disc diffusion and the antifungal screening was done in the same way against Candida albicans. The Thymus Vulgaris EO collected from Oran was very active against Staphylococcus aureus and Candida albicans. Lavandula angustifolia EO collected from Oran was extremely active against Pseudomonas aeruginosa and Candida albicans, Candida albicans was the most sensitive to the essential oils. Essential oils of L. angustifolia and T. vulgaris are highly effective natural antibacterial and antifungal agents that can be used to treat infections and food poisoning.

In-vitro antimicrobial activity of essential oils of Tasmania

ABSTRACT Thirteen commercial Tasmanian essential oils were evaluated for their antimicrobial activity against 14 human pathogens and cosmetic contaminants including Enterococcus hirae, Kocuria rhizophila, Staphylococcus epidermidis, Propionibacterium acnes, Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes, Klebsiella pneumoniae, Pluralibacter gergoviae, Pseudomonas fluorescens, Burkholderia cepacia, Pseudomonas putida, Aspergillus brasiliensis, and Aspergillus fumigatus. The antimicrobial activities of various essential oils were evaluated using the AlamarBlue Cell Viability Assay, covering a testing concentration range from 3.125 to 800 µg/mL. Essential oils were deemed active if their minimum inhibitory concentration (MIC) value was less than or equal to 800 µg/mL. The essential oils of Rosemary (Rosmarinus officinalis ct. α-pinene), Parsley Herb (Petroselinum crispum), and Bitter Fennels (Foeniculum vulgare) demonstrated significant growth inhibitory effects on one microorganism (P. fluorescens) within an MIC value of ≤ 800 µg/mL. Big Badja Gum (Eucalyptus badjensis), Blue Gum (Eucalyptus globulus), Peppermint (Mentha piperita), and Lavender (Lavandula angustifolia) essential oils inhibited the growth of two different microorganisms. Southern Rosalina (Melaleuca ericifolia), Parsley Seed (Petroselinum crispum), Smoky Tea Tree (Leptospermum glaucescens), and Kunzea (Kunzea ambigua) essential oils exhibited an MIC value of less than or equal to 800 µg/mL against 4, 5, 6, and 8 microorganisms respectively. Notably, Coastal Tea Tree (Leptospermum laevigatum) essential oil displayed the most profound antimicrobial activity, inhibiting 11 out of 15 microbial strains within an MIC range of 50–800 µg/mL. The results of the gas chromatography analysis showed that the coastal tea tree essential oil was composed primarily of monoterpenes, with smaller amounts of sesquiterpenes and their oxygenated derivatives. 35 Compounds were identified, representing 95.50% of the compounds in the coastal tea tree essential oil. The main constituents of this essential oil were α-pinene (34.83%), followed by α- and β-eudesmols (10.21% total), and limonene (9.56%).

Effect of a lavender essential oil and sleep hygiene protocol on insomnia in postmenopausal women: A pilot randomized clinical trial

IntroductionInsomnia and vasomotor symptoms are frequently experienced during the menopausal transition and in postmenopause, worsening sleep maintenance and quality of life. Our study evaluated the use of lavender essential oil and sleep hygiene guidance on quality of life, sleep patterns and self-reported hot flashes in postmenopausal women with insomnia diagnosis. MethodsThis study is part of a larger experimental, double-blind, randomized controlled trial. We included 35 postmenopausal women with a diagnosis of insomnia who were distributed in two groups: A Placebo group (sunflower oil) and an Aroma group (Lavandula angustifolia essential oil); both inhaled the oils before bedtime for 29 days. The groups received sleep hygiene guidelines and weekly follow-up. Validated questionnaires were used to assess the effect of the intervention, and a sleep diary was used to assess sleep onset latency (SOL), total sleep time (TST), and sleep efficiency (SE) daily. Participants who complained of vasomotor symptoms at baseline kept a daily record of their frequency and intensity. ResultsAll participants benefited in almost all domains of quality of life and in the self-assessed TST and SE. The Aroma group achieved a significant improvement in their overall quality of life score over time. No differences were observed in respect of the hot flashes record and daytime sleepiness. ConclusionsThe aromatherapy intervention was effective in improving the overall quality of life in the Aroma group. Moreover, the sleep diary and sleep hygiene instructions helped all participants to, respectively, self-assess and improve their sleep patterns.

Enhancing terpene and other plant volatiles analysis – A free spreadsheet tool “Retentify” for GC–MS data processing

Despite significant progress in gas chromatography-mass spectrometry (GC–MS), misidentifications still occur, especially in untargeted analyses of complex samples of plant origin. In addition to using reference materials, a common approach to confirm identifications is to employ linear retention indices (LRIs). Ideally, when LRI validation is made automatically by the software using accurate library data. However, present solutions in this area either require additional payment or have many constraints. As a result, reference LRIs are often not included in search algorithms, or if they are, library LRI values may not be matched to the selectivity of the column used. Many GC–MS analyses are conducted without LRI comparison, or the comparison is performed after an MS library search. Such a manual examination is highly laborious, especially for datasets containing hundreds or more components. Therefore, an easy-to-use spreadsheet tool was developed to be used after an MS library search. The tool called Retentify, among other features, can: (1) calculate the experimental RIs, (2) retrieve automatically reference RIs from the reliable compilation of Babushok et al. (NIST), considering an appropriate selectivity of the column, (3) compute experimental LRIs errors and indicate the potential mismatch in the red/yellow/green color scale. Retentify is thoroughly described and demonstrated in the analysis of true lavender (Lavandula angustifolia) essential oil. The uniqueness of this tool lies in its reliance on highly accurate, averaged retention data provided by NIST, automatic input of reference LRIs based on CAS, and visuals to help LRI evaluation. The tool is easy to use and requires no financial and minimal time investment to learn, making it accessible to any researcher without specialization in plant volatile analysis.

0410 Lavender essential oil and sleep hygiene as therapeutic options for insomniac postmenopausal women: a randomized clinical trial

Abstract Introduction Menopausal transition and postmenopause often bring sleep complaints and insomnia, a common sleep disorder. In addition, women at this stage also experience vasomotor symptoms, which can compromise sleep maintenance and quality of life. This study aimed to observe the effect of an intervention with aromatherapy and sleep hygiene in the quality of life, self-related sleep pattern and hot flashes record in postmenopausal women with insomnia diagnosis. Methods This pilot study is a double-blind randomized clinical trial. Of the 135 women interested in participating in the study, 34 postmenopausal women met the inclusion criteria and completed the protocol. Volunteers were clinically diagnosed with insomnia and distributed in two groups: Aroma Group (Lavandula angustifolia essential oil) (n=17) and Placebo Group (sunflower oil) (n=17), both inhaled before bedtime for 29 days. The groups received sleep hygiene guidelines and were monitored weekly. To assess the effect of the intervention, the Menopause-Specific Quality-of-Life Questionnaire, intervention version (MENQOL-I), and Epworth Sleepiness Scale (ESS) were used. With data from the sleep diary, we evaluated sleep onset latency (SLO), total sleep time (TST) and sleep efficiency (SE) daily. Additionally, participants who complained of vasomotor symptoms were invited to fill out a hot flashes form, reporting the presence or absence of these symptoms, daily amount, and its intensity. Results The therapeutic approaches in this study benefited participants in both groups in almost all domains of quality of life and self-rated sleep patterns such as TST and SE. Particularly, the lavender essential oil intervention provided significant improvement in the overall quality of life score over time. No differences were observed considering the hot flashes record and sleepiness. Conclusion The aromatherapy intervention was effective in improving overall quality of life in the Aroma group. Furthermore, strategies such as sleep hygiene and a sleep diary helped participants to self-assess their sleep routine and improve it. Support (if any) This research was supported by fellowships from Associação Fundo de Incentivo à Pesquisa (AFIP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) - Finance Code 001, and Conselho Nacional de Pesquisa (CNPq). Laszlo (essential oils, books, foods, and aromatherapy products) donated the necessary oils for this project.

Alginate nanoparticles containing Lavandula angustifolia essential oil as a potential potent, biocompatible and low-cost antitumor agent

Alginate nanoparticles containing Lavandula angustifolia essential oil as a potential potent, biocompatible and low-cost antitumor agent

Lavandula angustifolia Essential Oils as Effective Enhancers of Fluconazole Antifungal Activity against Candida albicans.

The increasing prevalence of Candida albicans resistance to commercial antifungal agents in recent decades has prompted modern medicine and veterinary medicine to search for combined treatment options. The aim of the study was to determine the activity of essential oils from different cultivars and morphological parts of the medicinal lavender (Lavandula angustifolia) in combination with fluconazole against Candida albicans ATCC 10231 strain. The effect of the combination of lavender essential oil with fluconazole was tested using the checkerboard method, and the obtained results were interpreted on the basis of fractional inhibitory concentration indices (FICIs). A synergistic interaction was found for all combinations of fluconazole with essential oils isolated both from flowers and leafy stalks of two tested lavender cultivars: 'Blue River' and 'Ellagance Purple'. The observed enhancement effect of fluconazole antifungal activity was significantly stronger in the case of essential oils obtained from flowers and leafy stalks of 'Blue River' cultivar. Analogous studies were performed for linalool, one of the main components of lavender essential oils, and a similar synergistic interaction with fluconazole was found.

The effects of Lavandula angustifolia essential oil on analgesic effects and percutaneous absorption of naproxen sodium gel; an in vivo and in vitro study.

The percutaneous bioavailability of naproxen is low and several technologies have been utilized to overcome the problem. Although, some studies have reported the permeation-enhancing properties of natural essential oils, no research has reflected the effectiveness of Lavandula angustifolia essential oil (LAEO) on increasing the percutaneous absorption of naproxen sodium from a topical gel. Therefore, the present study was designed to investigate whether LAEO increased the percutaneous absorption and the analgesic effects of naproxen sodium topical gel. In the present study, naproxen topical gel was formulated using carbopol 940 (a gelling agent) and several vehicles such as PEG 400, ethanol, and water and the properties of gels were measured. Percutaneous absorption-enhancing properties of LAEO were measured too. Based on our data, the essential oil-containing formulation of naproxen represented greater penetration into (222.19 ± 24.87 vs. 107.65 ± 6.38 μg/cm2 ), and also across (22.07 ± 4.42 vs. 13.14 ± 2.87 μg/cm2 ) skin layers compared to the naproxen gel. Additionally, a significant analgesic property was observed in the naproxen topical gel containing 0.5% essential oil during both first and late phases of formalin test, as well as the late phase of tail-flick test. It could be concluded that LAEO significantly enhanced naproxen percutaneous absorption and also its analgesic effects.

Lavandula austroapennina and Lavandula angustifolia essential oils and bioactive components: in vitro anti-denaturation effect of lavender from the Pollino massif (Southern Italy)

The Mediterranean basin represents a rich source of medicinal and aromatic plants. These species contain different metabolites such as phenolics, flavonoids, tannins, coumarins and sterols known for their beneficial properties. The essential oils (EOs) of Lavandula austroapennina and Lavandula angustifolia from the Pollino massif (southern Italy) were characterized using gas chromatography-mass spectrometry and their anti-denaturation effect was assessed using in vitro models with heat-treated bovine serum albumin (BSA) chosen as a protein model. L. austroapennina EO showed a better in vitro anti-denaturation activity compared to L. angustifolia, with IC50 values equal to 260.4 ± 4.2 and 480.0 ± 2.6 µg/mL, respectively. In order to relate the observed results to the most interesting identified phytochemicals, some major components were also tested. Linalool and terpinen-4-ol proved to be effective in protecting BSA from heat denaturation. Moreover, the inhibitory properties on nitric oxide production were verified in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Both lavender EOs showed concentration-dependent inhibitory properties, as well as the monoterpene linalool. Taken together, these results suggest that L. austroapennina EO and its main constituent linalool could be good candidates for further investigations, aimed at finding new drugs with anti-arthritic potential.

In vitro antibacterial effect of enrofloxacin combined with lavender essential oil on selected Salmonella serotypes isolated most commonly poultry

The present study investigated the antimicrobial activity of enrofloxacin in combination with lavender (Lavandula angustifolia) essential oil against three serotypes of Salmonella: Salmonella Enteritidis ATCC 13076, Salmonella Gallinarum biovar Pullorum ATCC 13036, Salmonella Typhimurium ATCC 14028 and Salmonella Typhimurium – a moderately sensitive monophasic strain. The analysis of the interaction of enrofloxacin with lavender essential oil was carried out using the checkerboard method. The obtained FICI values were interpreted as follows: synergistic when ≤ 0.5; additive when > 0.5 but ≤ 1; non-interactive when > 1 but ≤ 4; antagonistic when > 4. When analysing individual combinations of enrofloxacin with lavender oil, a positive additive effect was found in all cases (S. Enteritidis ATCC 13076 FICI – 0.85; S. Gallinarum biovar Pullorum ATCC 13036 FICI – 1.0; S. Typhimurium ATCC 14028 and S. Typhimurium – a moderately sensitive monophasic strain FICI – 0.7). Antagonistic interaction was not recorded in any case. When analysing individual combinations of enrofloxacin with lavender oil against the serotypes tested, an additive effect was demonstrated in all cases (FIC indices: 0.7-1.0). There was no antagonistic effect in any of the analysed combinations.