Particles and bacteria released from operators in pharmaceutical cleanrooms significantly impact the quality of pharmaceutical products. Therefore, it is necessary to study particle and bacteria emissions from operators and their garments. This paper presents the results of experimental measurements of numbers of particles and bacterial emissions from integrated and split cleanroom garments, under different movement types and laundry conditions. The experiment demonstrated that the emission rate of 0.5 μm particles from split-type garments is approximately 2.0–3.5 times higher than that of integrated cleanroom garments, and 7.0–22.7 times for 5.0 μm particles. The particle emission rate of humans is closely related to the movement types, number of laundry cycles, and garment types. The maximum particle emission rates were obtained when wearing split cleanroom garment after 100 washes and performing knee bend, with 654603 P/min for 0.5 μm and 27185 P/min for 5.0 μm particles, respectively. The particle emission rate of humans is mainly caused by personnel movement, since the emission rate of garments accounts for at most 3.89 %. The bacteria colony of humans increases with the increase of laundry cycles of cleanroom garments. The maximum bacteria colony of integrated and split cleanroom garment were 4 CFU/plate and 9 CFU/plate with the laundry cycles of 100 times. The quantitative results of bacteria and particles emitted from pharmaceutical cleanroom operators are expected to ensure drug quality during production.