Recently, the possible existence of negative gluon helicity Δg has been observed to be compatible with existing empirical constraints, including from jet production in polarized proton-proton collisions at the Relativistic Heavy Ion Collider, and lattice QCD data on polarized gluon Ioffe time distributions. We perform a new global analysis of polarized parton distributions in the proton with new constraints from the high-x region of deep-inelastic scattering (DIS). A dramatic reduction in the quality of the fit for the negative Δg replicas compared to those with positive Δg suggests that the negative Δg solution cannot simultaneously account for high-x polarized DIS data along with lattice and polarized jet data.