Background: Atherosclerotic cardiovascular disease (ASCVD) is the leading cause of death in women in the United States and risk factors increase with menopause. Midlife Latinas have a more adverse ASCVD risk profile than non-Latina White women but remain underrepresented in research. Identifying risk factors and assessing outcomes are necessary to address the primary prevention of ASCVD in this population. Thus, the aim of this analysis was to identify factors related to 10-year ASCVD risk in midlife Latinas. Methods: We conducted a cross-sectional analysis using baseline data from 30 participants enrolled in a pilot study designed to reduce ASCVD risk in perimenopausal Latinas. The women were aged 40-60 years, free of ASCVD, and without hormone therapy use in the previous three months. Sociocultural, behavioral, and reproductive factors (e.g., age, education, acculturation, perceived stress, physical activity, age at menarche) were self-reported using an interviewer-administered questionnaire. Carotid-femoral pulse wave velocity (cfPWV) and physical and anthropometric measures (e.g., weight, waist circumference, blood pressure, lipid profile) were collected during a clinical exam. The 10-year ASCVD risk score was determined using the American College of Cardiology ASCVD Risk Estimator tool. We assessed factors related to 10-year ASCVD risk using linear regression models and adjusted for factors associated with the outcome at p<0.1. Results: Participants were on average aged 46.8 ± 4.2 years, 48% had less than a high school education, and 97% were born outside of the continental United States. The mean cfPWV was 6.8 ± 1.1 m/s, and the mean 10-year ASCVD risk was 1.4% ± 0.97. In fully adjusted models, we found that higher cfPWV (ß [SE]= 0.48 [0.22], p=0.04) and experiencing any vasomotor symptoms in the previous two weeks (ß [SE]= 1.4 [0.44], p=0.005) were associated with greater 10-year ASCVD risk. Conclusions: Though the 10-year ASCVD risk was relatively low (<5%), we found that increased cfPWV and a report of vasomotor symptoms (hot flashes, night sweats) were associated with greater 10-year ASCVD risk in perimenopausal Latinas. These preliminary data indicate that cfPWV and vasomotor symptoms may be important factors to consider for early risk stratification in midlife Latina women.