Latifolia Essential Oil Research Articles

Fish Scale Gelatin Nanofibers with Helichrysum italicum and Lavandula latifolia Essential Oils for Bioactive Wound-Healing Dressings.

Essential oils are valuable alternatives to synthetic antibiotics that have the potential to avoid the pathogen resistance side effects generated by leather. Helichrysum italicum and Lavandula latifolia essential oils combined with fish scale gelatin were electrospun using a coaxial technique to design new bioactive materials for skin wound dressings fabrication. Fish scale gelatins were extracted from carp fish scales using two variants of the same method, with and without ethylenediaminetetraacetic acid (EDTA). Both variants showed very good electrospinning properties when dissolved in acetic acid solvent. Fish scale gelatin nanofibers with Helichrysum italicum and Lavandula latifolia essential oil emulsions ensured low microbial load (under 100 CFU/g of total number of aerobic microorganisms and total number of yeasts and filamentous fungi) and the absence of Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536, and Candida albicans ATCC 1023 as compared to fish scale gelatin without essential oils, which recommends them for pharmaceutical or topical applications. A scratch-test performed on human dermal fibroblasts proved that the biomaterials contributing to the wound healing process included fish scale gelatin nanofibers without EDTA (0.5% and 1%), fish scale gelatin nanofibers without EDTA and Lavandula latifolia essential oil emulsion (1%), fish scale gelatin nanofibers with EDTA (0.6%), and fish scale gelatin nanofibers with EDTA with Helichrysum italicum essential oil emulsion (1% and 2%).

Comparison of volatile compounds and antibacterial activity of Citrus aurantifolia, Citrus latifolia, and Citrus hystrix shell essential oils by pilot extraction

Citrus peel essential oil has considerable potential to be used as a direct agent against a wide range of Gram-positive and Gram-negative bacteria. The three citrus peel species used in this study included Citrus aurantifolia, Citrus latifolia, and Citrus hystrix. Essential oils were extracted on a pilot model and analyzed by gas chromatography-mass spectrometry (GC-MS). Gram-positive bacteria (Bacillus subilits, Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Samonella typhimurium, Pseudomonas aeruginosa) were used to counteract the antibacterial effects of the samples. The yield of hydrodistillation distilled essential oil is C aurantifolia (1.15%), C latifolia (1.32%), and C hystrix (3.18%). The essential oil contains the main ingredient D-Limonene. The highest antibacterial activity against L. monocytogenes is Citrus latifolia essential oil. The main chemical composition of essential oils are β-pinene, D-limonene, γ-terpinene, terpinolene, α-terpineol, … and the antimicrobial activity of the essential oil is affected by the variation of D-limonene. and β-pinene in essential oils.

Dietary limonCitrus × latifoliafruit peel essential oil improves antioxidant capacity of tambaqui (Colossoma macropomum) juveniles

The effects of dietary supplementation with Citrus × latifolia essential oil (EOCL: 0, 0.25, 0.5, 1.0 and 2.0 ml EOCL/kg diet) on growth, survival, gut tract morphology and the metabolic and oxidative parameters of tambaqui (Colossoma macropomum) were investigated in a 60-day experiment. The inclusion of up to 2.0 ml EOCL/kg diet did not promote growth; however, fish fed 1.0 and 2.0 ml EOCL/ kg diet presented higher survival and all EOCL groups had increased intestinal fold height and length compared to the control group. After 60 days of experiment, glucose, glycogen, lactate and protein levels in the liver and muscle were altered significantly by dietary addition of EOCL. The muscle LPO content was higher in fish fed 2.0 ml EOCL/ kg diet than the control group. The reactive oxygen species content was higher in the liver but lower in the muscle of fish fed 1.0 ml EOCL/kg diet compared to the control group. SOD activity was higher in the liver of fish fed 0.50 and 1.0 ml EOCL/kg diet than the control group. Therefore, dietary addition of 1.0 ml EOCL/kg diet is advisable for tambaqui juveniles since it improved survival, the antioxidant capacity of tissues and the intestinal absorption area.

Chemical Composition of Essential Oil of Madhuca longifolia var. latifolia (Roxb.) A. Chev. Flowers

In the present investigation, the chemical composition of essential oil of Madhuca longifolia var. latifolia (Roxb.) A.Chev. flowers was studied for its aroma compounds. The essential oil was isolated using hydrodistillation method. The chemical composition of isolated essential oil was investigated by using gas chromatography equipped with flame ionization detector (GC) and gas chromatography coupled with mass spectrometry (GC/MS) which identified total 25 compounds. The major constituents found in the essential oil of M. longifolia var. latifolia flowers are (E,E)-Farnesol (29.56%), (Z,E)-α-Farnesene (12.84%), Dendrolasin (8.95%), 2,3-Dihydrofarnesol (8.69%), (E,E)-α-Farnesene (7.37%), Nonanal (5.69%), and β-Farnesene (4.38%). The results indicated that the M. longifolia var. latifolia essential oil was a rich source of sesquiterpenes.

Poiretia latifolia essential oil as a promising antifungal and anti-inflammatory agent: Chemical composition, biological screening, and development of a nanoemulsion formulation

Poiretia latifolia is a Brazilian plant species used in traditional medicine for a wide range of ailments. The objective of this study was to evaluate the antioxidant potential, anti-inflammatory activity, and antifungal effect of P. latifolia essential oil and its major compounds. In addition, a nanoemulsified system was developed for its application. Aerial parts of the plant, collected at different stages of development, were hydrodistilled, and the chemical composition of the oil was determined by gas chromatography–mass spectrometry (GC–MS). Limonene, trans-dihydrocarvone, and carvone were identified as major compounds. Antioxidant potential was evaluated by three different methods: TBARS, β-carotene/linoleic acid, and DPPH. P. latifolia oil showed antioxidant activity (0.508 mg/mL) in lipophilic medium, as evidenced by the β-carotene/linoleic acid assay. The anti-chemotactic effect of the oil was evaluated by inhibition of neutrophil migration (0.001–10 μg/mL): P. latifolia oil was able to inhibit up to 94.7% of migration. Antifungal activity was evaluated against yeasts (Candida spp.) and dermatophytic filamentous fungi (Microsporum spp. and Trichophyton spp.). P. latifolia oil showed antifungal activity against Microsporum canis, and a mixture of carvone and limonene was effective against the dermatophytes Trichophyton rubrum and Trichophyton mentagrophytes. Nanoemulsions were successfully developed. P. latifolia oil has the potential to be used as an antidermatophytic and anti-inflammatory agent.

Fractionation of acid lime essential oil using ethanol/water mixtures: Effect of the process on the aroma profile

This study aims to separate aroma-active components of the crude Citrus latifolia essential oil (EO) from the unstable terpene hydrocarbons using ethanol/water mixtures through liquid–liquid equilibrium (LLE) and to evaluate the aroma profiles of the crude EO and the LLE phases. For this purpose, the liquid compositions of the crude EOs and the LLE phases were found by gas chromatography analysis. The compositions of the liquid phases were predicted using the NRTL model and compared to the experimental data. Afterwards, the concentrations of the components in the vapour phases above the liquid mixtures were obtained by headspace analysis and the odour intensity of each component was estimated using the Stevens’ power law concept. Finally, the classification into olfactory families was evaluated through the Perfumery Radar methodology. The solvent extraction technique made it possible to obtain extract phases enriched in citral and poor in monoterpenes, with aromas profiles similar to that of the crude EO and classified as herbaceous and citrus scent, with floral, woody, and oriental nuances. The extract phase obtained from ethanol with 50% water was found to be a promising fraction for industrial applications. Furthermore, the results proved that the NRTL parameters can be efficiently used to predict the compositions of the phases from the LLE.

Antimycotic Activity and Genotoxic Evaluation of Citrus sinensis and Citrus latifolia Essential Oils.

The aim of this study was to evaluate the antifungal activity of essential oils (EOs) of Citrus sinensis (C. sinensis) and Citrus latifolia (C. latifolia) against five Candida species: Candida albicans, Candida tropicalis, Candida glabrata, Candida lusitaniae and Candida guilliermondii; and perform its genotoxic evaluation. The EOs of C. sinensis and C. latifolia were obtained from the peel by hydro-distillation. The major components determined by GC-MS were in C. sinensis, d-limonene (96%) and α-myrcene (2.79%); and in C. latifolia, d-limonene (51.64%), β-thujene (14.85%), β-pinene (12.79%) and γ-terpinene (12.8%). Antifungal properties were studied by agar diffusion method, where C. sinensis presented low activity and C. latifolia essential oil was effective to inhibit growing of C. lusitaniae and C. guilliermondii with IC50 of 6.90 and 2.92 μg respectively. The minimum inhibitory concentrations (MIC) for C. sinensis were in a range of 0.42–3.71 μg and for C. latifolia of 0.22–1.30 μg. Genotoxic evaluation was done by Ames test where none of the oils induced point mutations. Flow cytometry was used to measure toxicity in human oral epithelial cells, C. sinensis was not cytotoxic and C. latifolia was toxic at 21.8 μg. These properties might bestow different odontological applications to each essential oil.

Insecticidal and oviposition deterrent effects of essential oils and their constituents against the invasive pest Drosophila suzukii (Matsumura) (Diptera: Drosophilidae)

Spotted wing drosophila (SWD), Drosophila suzukii (Matsumura), is an important new invasive pest of stone and berry fruits in North America and current control methods require frequent application of synthetic pesticides. This has created a need for new and environmentally friendly biopesticides for the control of SWD. This paper investigated the potential of nine essential oils from avocado (Persea americana Mill.), neem (Azadirachta indica A. Juss), kukui nut (Aleurites moluccana L.), macadamia nut (Macadamia integrifolia Maiden & Betche), spike lavender (Lavandula latifolia Vill.), Grosso lavandin leaf and flower (Lavandula×intermedia cv ‘Grosso’), and Provence lavandin leaf and flower (Lavandula×intermedia cv ‘Provence’) as well as three major monoterpene constituents of lavender essential oils: 1,8-cineole, 3-carene and linalool for their ability to control SWD through fumigation and contact toxicity assays as well as oviposition deterrent activity. Linalool was found to be the most effective monoterpene in fumigation assays (EC50 1.85 μL/L air) and spike lavender floral essential oil was found to be the most effective whole oil (EC50 3.79 μL oil/L air). In contact toxicity assays 1,8-cineole (EC50 0.67%) was the most effective monoterpene while avocado (EC50 0.54%) and spike lavender (EC50 0.69%) oils were the most effective whole oils. No significant oviposition deterrent activity was observed. This report indicates that L. latifolia essential oil and commercially available avocado oil are strong lead candidates for management of SWD. Furthermore the activity of L. latifolia essential oil is likely due to the high content of 1,8-cineole and linalool. This is the first report of significant insecticidal activity by these oils and compounds against SWD.

By-product of Lavandula latifolia essential oil distillation as source of antioxidants

The objective of this work was to evaluate the antioxidant properties of Lavandula latifolia waste obtained after essential oil distillation. Samples of 12 wild populations of the Lavandula genus collected between 2009 and 2010 were hydrodistilled and their by-products were analyzed using the Folin–Ciocalteu, free radical scavenging activity (2,2-diphenyl-1-picrylhydrazyl), and the ferric reducing antioxidant power (FRAP) methods. Rosmarinic acid, apigenin, and luteolin contents were analyzed by high-performance liquid chromatography–diode array detection. The mean of total phenolic content ranged from 1.89 ± 0.09 mg gallic acid equivalents/g dry weight to 3.54 ± 0.22 mg gallic acid equivalents/g dry weight. The average value of the half maximal effective concentration (EC50) for scavenging activity ranged from 5.09 ± 0.17 mg/mL to 14.30 ± 1.90 mg/mL and the variability of the EC50 in FRAP ranged from 3.72 ± 0.12 mg/mL to 18.55 ± 0.77 mg/mL. Annual variation was found among this samples and the environmental conditions of 2009 were found to be more favorable. The plants collected from Sedano showed the highest antioxidant power. Our results show that rosmarinic acid and apigenin in L. latifolia contributed to the antioxidant properties of the waste. In conclusion, the by-product of the distillation industry could be valorizing as a source of natural antioxidants.

Influence of spike lavender (Lavandula latifolia Med.) essential oil in the quality, stability and composition of soybean oil during microwave heating

Lipids oxidation is one of the main factors leading to quality losses in foods. Its prevention or delay could be obtained by the addition of antioxidants. In this sense the present work intend to monitor the protective effects of Lavandula latifolia essential oil during soybean oil microwave heating. To achieve the proposed goal quality parameters (free acidity, peroxide value, specific coefficients of extinction and ΔK), fatty acids profile, tocopherols and tocotrienols composition, antioxidant activity and oxidative stability were evaluated in soybean oil with and without spike lavender essential oils (EO) submitted to different microwave heating exposure times (1, 3, 5, 10 and 15min; 1000Watt) with a standard domestic microwave equipment. Microwave heating induced severe quality and composition losses, mainly above 3min of microwave heating, regardless the sample tested. However, spike lavender EO addition counteracts the oxidation comparatively to control oils, by presenting enhanced values in quality parameters. A higher protection in unsaturated fatty acids loss was also observed as well as a higher antioxidant activity and oxidative stability. The microwave heating effects were clearly different in the samples with essential oils addition, allowing discrimination from plain soybean oils by a principal component analysis, being also capable to discriminate the different heating times tested within each sample.

Antiradical potential and antifungal activities of essential oils of the leaves of Citrus latifolia against Phaeoramularia angolensis

Investigations were conducted to determine the chemical composition, antiradical and antifungal activities of the essential oil extracted from the fresh leaves of Citrus latifolia var. Tahiti from Cameroon against Phaeoramularia angolensis. The essential oil obtained by hydrodistillation was analysed by GC and GC/MS. The disc diffusion method was used to evaluate the zone of fungal growth inhibition at various concentrations of the oil while the antiradical activity of the oil was studied by the DPPH (diphenyl picryl hydrazyl) method. The chemical analysis revealed 26 components among which limonene (45.76%), geranial (13.12%) and neral (10.35%) were the main components. The antiradical activity of C. latifolia essential oil (SC50 = 9.93 g/l) was less than that of butylated hydroxyl toluene (BHT) which was used as the reference compound (SC50 = 7.02 x 10-3 g/l). After 40 days of incubation on oil – supplemented medium, the growth of P. angolensis was totally inhibited by 1600 mg/l of C. latifolia oil. Results obtained in the present study indicate the possibility of exploiting C. latifolia var Tahiti essential oil to combat P. angolensis which is responsible for heavy losses of Citrus fruits harvests.

Biological Activity of Heterotheca latifolia Essential Oil Against Varroa jacobsoni

The acaricidal effects and adult bee toxicity of Heterotheca latifolia essential oil on Varroa jacobsoni mites were evaluated. The oil was prepared as an emulsion and applied on the varroa females and on adult bees by pulverization in Burgerjon's tower. Varroa lethality test was determined by 3%, 4% and 5% concentrations. The active ingredient was sprayed on the mites placed in groups of 10 on filter papers placed on a plastic dish. As controls, distilled water and water with the emulsifier were used. After spraying, mites were placed in an incubator at 33°±1°C and 70% RH. At these concentrations (3%, 4% and 5%) the oil showed a high mite mortality, while in the control group (water and water + emulsifier) the mortality was lower. Significant differences between control groups and tested groups were observed (p<0.05). At the highest concentrations (5%) H. latifolia oil did not show bee toxicity. Significant differences between control and tested groups were not observed (p>().()5). These results suggested that H. latifolia oil might play an important role in this honeybee parasitosis management.

Constituents of the essential oil of Lavandula latifolia

Thirty components were identified in Lavandula latifolia essential oil (spike oil). One of the compounds, espliegol (δ-terpineol), is a new natural product.