India being a rich and varied flora of medicinal plants since the Vedic period. The present study deals with the scientific validation of P. daemia.Linn with special reference to its pharmacognostical and phytochemical investigations. This is commonly known as P. daemia, figure and is known to have medicinal properties, also even used as home remedies in the rural and the remotest parts of the India Qualitative & Quantitative analysis for nutritional value and amino acid detection for both sample has been estimated & detected TLC finger prints of latex of at 366 nm (before derivatization) & Rf values in TLC finger prints of latex at 254 nm (after derivatization) has been studied for both Samples. Latex is applied externally on chronic infected wounds to alleviate edema, pain and to promote the healing; therefore an attempt has been made to carried out the detailed quality control and assurance of the drug followed by HPTLC profiles, HPTLC profile of P. Daemia latex milk powder observed under 366 nm & 254 nm shows the variety of results A – C (Gautala forest,) B - Aurangabad (M.S.) Sample A, B & C.