Lateral Strip Research Articles

Mapping the quadriceps tendon: an anatomic and morphometric study to guide tendon harvesting.

The quadriceps tendon (QT) may be used in first-line knee ligament surgery (Anterior and Posterior Cruciate Ligaments), surgical revision and multiligament surgery. There are few published anatomic guides to QT harvesting. The present anatomic study sought to determine the ideal harvesting site and exa mined possible correlation between patellar and graft sizes. A descriptive morphometric anatomic study was performed on 12 cadaveric knees. The reference anatomical landmark was the center of the superior edge of the patella. The QT was dissected and sliced longitudinally into five 5-mm strips. The central strip corresponded to the anatomic center of the patella. QT thickness was measured every 10 mm over a length of 100 mm. Data were analyzed on Pearson correlation test and Student, Bartlett and Fisher tests (α risk = 0.05). QT thickness ranged from 0.7 to 9.78 mm, for a mean 4.94 mm. Mean thickness in the lateral, central and medial strips was, respectively, 3.464, 6.040 and 3.899 mm. Central and centromedial strips were thicker than medial, centrolateral and lateral strips; central and centromedial strips were similar at, respectively, 6.040 and 6.041 mm (non-significant: p = 0.95), and significantly thicker than lateral strips. QT thickness showed significant correlation with patellar length (r = 0.75; p = 0.0048; 95% CI [+0.31; +0.93]). The present anatomical study confirmed that QT should be harvested from the central and centromedial regions. Mean thickness was 7.84 mm at the patellar insertion, 7.37 mm at 20 mm from the insertion, 6.41 at 40 mm, 5.61 at 60 mm and 4.33 at 100 mm.

Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples

It is a fact that the level of estradiol (E2) in powdered milk has been increased manyfold with growing concerns on dairy safety. Our study produced a panel of monoclonal antibodies (mAb) specific for E2 screening and established a lateral flow colloidal gold immunoassay strip for determining E2 in milk samples with the E2 mAb. The sensitivity of the test strip was found to be 180.42 ± 3.17 pg/mL and the lower detection limit (LDL) value was 37.14 pg/mL. For negative milk samples spiked with 400, 800, and 1200 pg/mL, the recovery range was 86.7–93.5% and the coefficient of variation scope [CV (%)] was 4.8–6.2%. The results of comparative studies between test strips and HPLC were relatively identical while the strip test only required less than 8 min. In the study, the test strip was a practical screening technology for quantitative, semi-quantitative, or qualitative detection of E2 residues.

Disposable lateral flow-through strip for smartphone-camera to quantitatively detect alkaline phosphatase activity in milk

A disposable lateral flow-through strip was developed for smartphone to fast one-step quantitatively detect alkaline phosphatase (ALP) activity in raw milk. The strip comprises two functional components, a conjugation pad loaded with phosphotyrosine-coated gold nanoparticles (AuNPs@Cys-Try-p) and a testing line coated with anti-phosphotryosine antibody (anti-Tyr-p mAb). The dephosphorylation activity of ALP at the testing zone can be quantitatively assayed by monitoring the accumulated AuNPs-induced color changes by smartphone camera, thus providing a highly convenient portable detection method. A trace amount of ALP as low as 0.1UL(-1) with a linear dynamic range of 0.1-150UL(-1) (R(2)=0.999) in pasteurized milk and raw milk can be one-step detected by the developed flow-through strip within 10min, demonstrating the potential of smartphone-based portable sensing device for pathogen detection. This bio-hazards free lateral flow-through testing strip can be also used to fabricate rapid, sensitive and inexpensive enzyme or immunosensors for broad portable clinic diagnosis and food contamination analysis, particularly in point-of-care and daily food quality inspection.

A one-piece lateral flow impedimetric test strip for label-free clenbuterol detection

A one-piece lateral flow impedance strip was developed for detection of clenbuterol hydrochloride, a restricted food additive.

Development of prostatic exosomal protein (PSEP) lateral flow chromatographic strips for detection of chronic prostatitis

Development of prostatic exosomal protein (PSEP) lateral flow chromatographic strips for detection of chronic prostatitis

RF Infrastructure Cooperative System for in Lane Vehicle Localization

This paper presents a sensor which can be included in an Advanced Driver Assistance System (ADAS) that is compliant with the Vehicle to Infrastructure communication standard (V2I). This system allows estimation of the vehicle lateral position in real time by ensuring cooperation between an on-board vehicle system and passive transponders integrated in the lateral white strips of the road. Based on an optimization method, the lateral position vehicle is provided with a distance error less than 3 cm. In this paper, experimental results are presented in order to evaluate the robustness of the proposed system in a realistic environment. Three scenarios are considered to take into account the bitumen properties, the presence of parasitic reflectors in different positions around the system and the interaction between transponders.

Real-time measurement of human salivary cortisol for the assessment of psychological stress using a smartphone

We present a simple smartphone-based measurement system consisting of a smartphone, a holder, and a lateral flow immune strip. The smartphone camera and light source were used to read the colorimetric signal from the lateral flow assay. A smartphone application was written and installed onto the smartphone. Various concentrations of cortisol were successfully measured using the images captured by the smartphone. Measurement of human salivary cortisol was then demonstrated using the lateral flow assay and the quantitative analysis was validated with the smartphone. The system was further evaluated using human saliva, demonstrating an accurate and reproducible platform for rapid and point-of-care quantification of cortisol using a smartphone-based measurement system.

Development of a simultaneous lateral flow strip test for the rapid and simple detection of deoxynivalenol and zearalenone.

The objective of this study was to develop a 1-step simultaneous lateral flow strip test for the rapid and simple detection of deoxynivalenol (DON) and zearalenone (ZEA) in grains. Two monoclonal antibodies (MAbs) against DON and ZEA were respectively conjugated with gold nanoparticles and used to develop a lateral flow strip test for a single toxin and multiple toxins. First, individual lateral flow strips for a single toxin were optimized, and their conditions were used to develop a simultaneous lateral flow strip for multiple toxins. Limits of detection of both lateral flow strip tests for DON and ZEA were the same (DON: 50 ng/mL, ZEA: 1 ng/mL). Both methods showed cross-reactivity for α-zearalenol and β-zearalenol, but no cross-reaction to other mycotoxins. The results can be completed obtained within 15 min. The cut-off values of the simultaneous lateral flow strip for the spiked rice and corn were 500 and 10 ng/g for DON and ZEA, respectively. The results demonstrated that the developed simultaneous lateral flow strip test offers a rapid, easy-to-use, and portable analytical system and can be used as a convenient qualitative tool for the on-site detection of DON and ZEA in food and agricultural commodities. Simultaneous lateral strip test is useful for a rapid detection of DON and ZEA at a time in food and grain samples.

Identification of Eye-Specific Domains and Their Relation to Callosal Connections in Primary Visual Cortex of Long Evans Rats.

Ocular dominance columns (ODCs) exist in many primates and carnivores, but it is believed that they do not exist in rodents. Using a combination of transneuronal tracing, in situ hybridization for Zif268 and electrophysiological recordings, we show that inputs from both eyes are largely segregated in the binocular region of V1 in Long Evans rats. We also show that, interposed between this binocular region and the lateral border of V1, there lies a strip of cortex that is strongly dominated by the contralateral eye. Finally, we show that callosal connections colocalize primarily with ipsilateral eye domains in the binocular region and with contralateral eye input in the lateral cortical strip, mirroring the relationship between patchy callosal connections and specific sets of ODCs described previously in the cat. Our results suggest that development of cortical modular architecture is more conserved among rodents, carnivores, and primates than previously thought.

A three-line lateral flow assay strip for the measurement of C-reactive protein covering a broad physiological concentration range in human sera

The lateral flow assay (LFA) strip sensor possesses many advantages as a diagnostic device, including the capabilities of rapid, one-step assay performance, and high throughput production. A major limitation of the sensor, however, is its difficulty in measuring a broad concentration range of target proteins, including C-reactive protein (CRP), due to the “hook effect.” In this study, we report the use of a three-line LFA strip sensor, adding an antigen line to the conventional two-line LFA sensor, for detecting CRP within a broad concentration range in human sera. We introduced an antigen line between test and control lines in the LFA sensor. The antigen line was formed by dispensing a CRP antibody solution followed by a CRP solution in nitrocellulose membrane. All other conditions were identical to those applied to the conventional LFA strip sensor. The CRP level in test samples was generated by data processing from the intensities of three lines. The strip sensor measured a linear detection range of CRP concentration from 1ng/mL to 500μg/mL within 10min, with a calculated detection range of 0.69ng/mL–1.02mg/mL. Using the developed three-line LFA sensor, 50 clinical samples were measured at a detection range of 0.4–84.7μg/mL. This novel and easy-to-use CRP sensor can be a useful tool for rapid, sensitive, and cost-effective detection of a broad physiological concentration range of CRP capabilities that are vital for various diagnostic applications.

Detection of transgenic events in maize using immunochromatographic strip test and conventional PCR

With the growth in the transgenic market, fast and economically viable methodologies are necessary for undertaking transgene detection tests, both for identification of contamination in seeds and in grain. Seeds from commercial conventional GNZ 2004, and transgenic VT-Pro (MON89034), Roundup Ready (NK603) and Herculex (TC1507) maize cultivars were used. In order to simulate different levels of contamination, the transgenic seeds were mixed with conventional seeds at levels of 0.2%, 0.4%, 1.0% and 1.6% for VT-Pro, and 0.2%, 0.5%, 0.8% and 1.2% for Roundup Ready and Herculex. The lateral flow membrane strip test was performed in the whole seed, endosperm and embryo. For evaluation of the specificity of the technique in detection of the TC1507 event by means of the conventional PCR technique, seeds of the commercial maize hybrid GNZ 2004 were used as negative control, and the maize hybrid 2B655Hx as positive control. In order to simulate different levels of contamination, transgenic seeds were mixed with conventional seeds at the levels of 10%, 5%, 1%, 0.5% and 0.1%. Seeds from each sample were crushed, and then DNA extraction was performed by the CTAB 2% method. Using the immunochromatographic strip, it was possible to evaluate the expression of proteins related to the VT-Pro, Roundup Ready and Herculex events when whole seeds were used at the 0.2% level of contamination, whereas by the conventional PCR technique, it was possible to detect the TC1507 event in samples with 1% contamination.

Anatomical and Functional Recovery of Neurotized Remnant Rectus Abdominis Muscle in Muscle-Sparing Pedicled Transverse Rectus Abdominis Musculocutaneous Flap

BackgroundPedicled transverse rectus abdominis musculocutaneous flaps typically sacrifice the entire muscle. In our experience, the lateral strip of the rectus abdominis muscle can be spared in an attempt to maintain function and reduce morbidity. When the intercostal nerves are injured, muscle atrophy appears with time. The severed intercostal nerve was reinserted into the remnant lateral strip of the rectus abdominis muscle to reduce muscle atrophy.MethodsThe authors retrospectively reviewed 9 neurotized cases and 10 non-neurotized cases. Abdominal computed tomography was performed to determine the area of the rectus muscles. Electromyography (EMG) was performed to check contractile function of the remnant muscle. A single investigator measured the mean areas of randomly selected locations (second lumbar spine) using ImageJ software in a series of 10 cross-sectional slices. We compared the Hounsfield unit (HU) pre- and postoperatively to evaluate regeneration quality.ResultsIn the neurotization group, 7 of 9 cases maintained the mass of remnant muscle. However, in the non-neurotization group, 8 of 10 lost their mass. The number of totally atrophied muscles in each of the two groups was significantly different (P=0.027). All of the remnant muscles showed contractile function on EMG. The 9 remaining remnant rectus abdominis muscles showed declined the HU value after surgery but also within a normal range of muscle.ConclusionsNeurotization was found to be effective in maintaining the mass of remnant muscle. Neurotized remnant muscle had contractile function on EMG and no fatty degeneration by HU value.

Strengthening of RC walls using externally bonding of steel strips

The purpose of this experimental study is to investigate the hysteretic behavior of shear deficient reinforced concrete (RC) shear walls that are strengthened by bonding of steel strips. Four, 1/2 scale shear wall test specimens were constructed and tested under cyclic lateral loading. The slenderness ratio of the wall (h/l, where h=wall height, l=wall length) was 1.5. For this experimental research three wall specimens were strengthened with different steel strips configurations. The different configurations of steel strips were considered the diagonal strip, lateral strip and the combination of both lateral and vertical strips. All steel strip configurations are arranged on both sides of the shear wall symmetrically. The research focused on the effect of using bonding steel strips enhanching strength and increasing ductility of the non-seismic detailed shear walls. Test results showed that all the steel strip configurations improved the lateral strength, energy dissipation capacity and deformation capacity of the shear deficient RC wall significantly. Strengthened specimens developed the nominal flexural strength, and hence, the observed maximum base shear was controlled by flexure. Steel strips limited the opening of shear cracks and improved the lateral displacement capacity.

Ovine Tests of a Novel Spinal Cord Neuromodulator and Dentate Ligament Fixation Method

ABSTRACTBackground: To improve methods for the treatment of intractable pain, we are developing a novel intradural spinal cord stimulator that could be either attached to the dentate ligaments of the human spinal cord or fitted around the dorsal arc of the cord itself. Purpose: Our goal was to carry out the first in vivo tests of these attachment methods in an ovine model using custom-built devices and instrumentation. For eventual translational studies, we also explored methods of mimicking a human dentate ligament attachment technique in this large animal model. Methods: As a starting point, we investigated details of the gross and histological anatomy of the ovine denticulate ligaments, and compared them with their human counterpart. The gap between the dura and the spinal cord in the sheep is small; hence, the denticulate ligaments are not long enough to accommodate human-scaled attachment clips. Therefore, lateral strips of the spinal-canal dura were fashioned to serve this same device attachment function. Results: This form of dural anchoring was implemented surgically for fixation of a silicone membrane implant that had 12 electrodes, and somatosensory evoked potentials were obtained successfully when stimuli were applied to it. The dorsal arc clamping technique was also implemented. Conclusions: We demonstrated that the dural attachment method is an effective surrogate model for testing the human dentate ligament device fixation technique, and that this mode of fixation was preferable to dorsal arc attachment. The relevant surgical innovations, anatomical findings, and the preliminary electrophysiological data from a pial surface stimulator attached in this way are presented.

Parametric Study of Dynamic Wrinkling in a Thin Sheet on Elastic Foundation

This work presents an approximate analytical study of the problem of dynamic wrinkling of a thin metal sheet under a specified time varying tension. The problem is investigated in the framework of the dynamic stability of a nonlinear plate model on elastic foundation which namely takes into account the nonlinear mechanics of mid-plane stretching and the dependence of the membrane force on this mechanics. The plate is assumed to be a wide rectangular slab, hinged at two opposite ends and free at the long ends, which can be deformed in a cylindrical shape so that the governing in-plane bending equation of motion takes the same form as that of a beam (e.g. lateral strip) element. An approximate analytical analysis of the beam wrinkling behavior under sinusoidal parametric excitation is carried out by using the assumed single mode wrinkling motion to reduce the beam field nonlinear partial differential equation to that of a single degree of freedom non-linear oscillator. A first order stability analysis of an approximate analytical solution obtained using the Multi-Time-Scales (MMS) method is used to derive a criterion defining critical driving frequency in terms of system parameters for the initiation of wrinkling motion in the thin metal sheet. Results obtained using this criterion is presented for selected values of system parameters.

Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Olaquindox Residues

A rapid immunochromatographic lateral flow test strip of competitive format has been developed for the specific determination of olaquindox (OLA) residues in pig urine and muscle tissues. The sensitivity of the test strip was found to be 1.58 ± 0.27 μg/kg and 1.70 ± 0.26 μg/kg of OLA in pig urine and muscle tissues, and the lower detection limit was 0.27 ± 0.08 μg/kg and 0.31 ± 0.07 μg/kg respectively. For negative pig urine and muscle samples spiked with 4, 12, and 36 μg/kg, the recovery range was 83.0-94.0% and 78.8-87.4% and the coefficient of variation scope [CV (%)] was 3.17-7.41% and 4.66-7.64% respectively. Parallel analysis of OLA samples from pig urine and muscle tissue showed comparable results from the test strip and HPLC. Each test requires 5-8 min, and the test strip can provide a useful screening method for quantitative, semiquantitative, or qualitative detection of OLA residues.

Immediate Eye Rehabilitation at the Time of Facial Nerve Sacrifice

In the setting of known facial nerve sacrifice or injury, patients require precautions to prevent exposure keratitis and the morbidity that follows. One recommended treatment is surgical placement of a gold weight with or without lateral tarsal strip. In patients in whom the facial nerve has been sacrificed, it is unknown whether rehabilitation should be simultaneous or in the perioperative period. Case series with chart review of patients who underwent immediate rehabilitation of the eye (gold weight and lateral tarsal strip) following facial nerve resection. From 1998 to 2009, 52 patients were studied. Postoperative ophthalmologic complications and the need for revision surgeries were measured. A gold weight was placed in all patients, and 48 of 52 (92%) simultaneous lateral tarsal strips were performed. The facial nerve was sacrificed in 51 of 52 (88%) patients, and the remaining patient had a known preoperative facial nerve paralysis. Thirty-six of 52 (69%) required free tissue transfer for reconstruction, underscoring the extensive resections performed. A 1.2-g gold weight was placed in 50 of 52 (96%) patients. Three (6%) patients required gold weight revision with a larger weight and 3 (6%) for extrusion. Eight (16.7%) patients underwent revision of the lateral tarsal strip for ectropion. No patients developed ophthalmologic complications. Patients undergoing radical surgical resections with known or suspected injury of the facial nerve should be considered for simultaneous rehabilitation of the upper and lower eye.

The correction of involutional entropion of eyelid by lateral strip procedure

Aim:To determine cosmetic and functional outcome following lateral strip procedure (LSP) for involutional entropion.Materials and Methods:This study was a prospective analysis of 15 patients (20 eyelids) of involutional entropion, who needed surgical repair. After thorough evaluation, the surgical treatment (LSP) was done in all 15 patients.Results:Cosmetic and functional outcome was excellent in all cases following LSP. No complications and recurrence were encountered in any case.Conclusions:LSP is simple, physiologic, easy and quick to perform as OPD procedure for involutional entropion under local anesthesia without hospitalization by a general ophthalmologist.

New species of Crotonia (Acari: Oribatida: Camisiidae) from Nothofagus and Eucalyptus forests in Victoria, Australia, with a redescription of the fossil species Crotonia ramus (Womersley, 1957)

Six new species of oribatid mite belonging to the genus Crotonia are described from wet forests in Victoria, Australia. Crotonia alpina sp. nov., C. cornuta sp. nov. and C. victoriae sp. nov. belong to the Capistrata species-group, having the full complement of notogastral setae in the c series; whilst C. momitoi sp. nov., C. blacki sp. nov. and C. gadubanudi sp. nov. are members of the Cophinaria species-group, lacking setae c 2 . The fossil species Crotonia ramus (Womersley, 1957), also a member of the Cophinaria group, is redescribed from Tertiary Kauri pine resin (Agathis yallournensis). The new members of the Capistrata group share an unique combination of characters, including long flagelliform setae c 3 , shorter setiform c 2 and with setae c 3 the shortest of the c series; lateral strips of the notogastral shield ornamented with fields of tubercles; narrow, blunt bothridial auriculae and elongate parallel apophyses of setae h 2 projecting horizontally. The morphological homogeneity of this cluster of species is mirrored by the members of the Cophinaria species-group described herein which, together with C. pyemaireneri Colloff, 2009 and C. tasmanica Łochyńska, 2008 from Tasmania, plus C. jethurmerae Lee, 1985 from South Australia, share relatively well-developed setae d 2 , a porose notogastral shield with narrow lateral tuberculate strips; elongate, acute bothridial auriculae; long, flagelliform setae p 1 and relatively short apophyses of setae h 2 , divergent apically, and projecting posteriodorsally. The Victorian members of the Capistrata andCophinaria species-groups represent two homogeneous clusters of species associated with temperate rainforest refugia and wet sclerophyll forest in high-rainfall zones. An identification key is provided to the Australian species of Crotonia.

Spontaneous release of acetylcholine from autonomic nerves in the bladder

Bladder contractility is regulated by intrinsic myogenic mechanisms interacting with autonomic nerves. In this study, we have investigated the physiological role of spontaneous release of acetylcholine in guinea pig and rat bladders. Conventional isotonic or pressure transducers were used to record contractile activity of guinea pig and rat bladders. Hyoscine (3 micromol x L(-1)), but not tetrodotoxin (TTX, 1 micromol x L(-1)), reduced basal tension, distension-evoked contractile activity and physostigmine (1 micromol x L(-1))-evoked contractions of the whole guinea pig bladder and muscle strips in vitro. omega-Conotoxin GVIA (0.3 micromol x L(-1)) did not affect physostigmine-induced contractions when given either alone or in combination with omega-agatoxin IVA (0.1 micromol x L(-1)) and SNX 482 (0.3 micromol x L(-1)). After 5 days in organotypic culture, when extrinsic nerves had significantly degenerated, the ability of physostigmine to induce contractions was reduced in the dorso-medial strips, but not in lateral strips (which have around 15 times more intramural neurones). Most muscle strips from adult rats lacked intramural neurones. After 5 days in culture, physostigmine-induced or electrical field stimulation-induced contractions of the rat bladder strips were greatly reduced. In anaesthetized rats, topical application of physostigmine (5-500 nmol) on the bladder produced a TTX-resistant tonic contraction that was abolished by atropine (4.4 micromol x kg(-1) i.v.). The data indicate that there is spontaneous TTX-resistant release of acetylcholine from autonomic cholinergic extrinsic and intrinsic nerves, which significantly affects bladder contractility. This release is resistant to blockade of N, P/Q and R type Ca(2+) channels.