Based on outcrop, seismic and drilling data, the main regional unconformities in the Sichuan Basin and their controls on hydrocarbon accumulation were systematically studied. Three findings are obtained. First, six regional stratigraphic unconformities are mainly developed in the Sichuan Basin, from the bottom up, which are between pre-Sinian and Sinian, between Sinian and Cambrian, between pre-Permian and Permian, between middle and upper Permian, between middle and upper Triassic, and between Triassic and Jurassic. Especially, 16 of 21 conventional (and tight) gas fields discovered are believed to have formed in relation to regional unconformities. Second, regional unconformity mainly controls hydrocarbon accumulation from five aspects: (1) The porosity and permeability of reservoirs under the unconformity are improved through weathering crust karstification to form large-scale karst reservoirs; (2) Good source-reservoir-caprock assemblage can form near the unconformity, which provides a basis for forming large gas field; (3) Regional unconformity may lead to stratigraphic pinch-out and rugged ancient landform, giving rise to a large area of stratigraphic and lithologic trap groups; (4) Regional unconformity provides a dominant channel for lateral migration of oil and gas; and (5) Regional unconformity is conducive to large-scale accumulation of oil and gas. Third, the areas related to regional unconformities are the exploration focus of large gas fields in the Sichuan Basin. The pre-Sinian is found with source rocks, reservoir rocks and other favorable conditions for the formation of large gas fields, and presents a large exploration potential. Thus, it is expected to be an important strategic replacement.