Lateral Dendrite Research Articles

Simulation of Dendrite Remelting via the Phase-Field Method

The solidification of alloys is a key physical phenomenon in advanced material-processing techniques including, but not limited to, casting and welding. Mastering and controlling the solidification process and the way in which microstructure evolution occurs constitute the key to obtaining excellent material properties. The microstructure of a solidified liquid metal is dominated by dendrites. The growth process of these dendrites is extremely sensitive to temperature changes, and even a small change in temperature can significantly affect the growth rate of the dendrite tip. Dendrite remelting is inevitable when the temperature exceeds the critical threshold. In this study, a temperature-induced-dendrite remelting model was established, which was implemented through the coupling of the phase field method (PFM) and finite difference method (FDM). The transient evolution law of dendrite remelting was revealed by simulating dendritic growth and remelting processes. The phase field model showed that the lateral dendrites melt first, the main dendrites melt later, and the main dendrites only shrink but do not melt when the lateral dendrites have not completely melted or the root is not broken. The long lateral branches break into fragments, while the short lateral branches shrink back into the main dendrites. The main dendrites fracture and melt in multiple stages due to inhomogeneity.

Phase-field simulation study on dendritic growth behavior during bilateral directional solidification

The directional solidification technique can uniform the crystal growth direction within the material, but does not remove the lateral side branches of the axial main arm during the growth process. The influence of such lateral side branches existing in the directionally solidified dendritic organisation on the mechanical properties of the material cannot be neglected. In this paper, based on the Kim-Kim-Suzuki (KKS) phase-field model, the competitive behaviour of lateral secondary dendrites between the two main arms under different boundary conditions and non-synchronous growth conditions is investigated for Fe-0.5 %C alloys. In addition, the diffusion and enrichment rules of solutes are analysed by the distribution of solutes. The results show that the molten pool is spherical without external force, and the size distribution of the molten pool is as follows: dendrite tip collision zone > dendrite root > dendrite wall, and the solute segregation rule is the same; The spacing of the main arms has little effect on the concentration values between the dendrites, and the solutes are very symmetrically over the entire computational region; The dendrite tip interface concentration during the stable phase of dendrite growth is 0.89 %. Without considering the initial liquid-phase concentration, the concentration in the collision zone at the dendrite tip is approximately twice the concentration at the tip of the dendrite in the stable period, which is 1.28 %, when the bilateral dendrites are encountered. Dendrites always collide preferentially on the centreline of the included angle under non-parallel conditions, and solute-enriched zones also occur on the centreline of the included angle; During the curved surface bilaterally directional solidification process, when the left and right sides have the same shape of surfaces, the solute enrichment at the end of solidification is on the centreline. For the case of a single planar boundary, the solute-enriched region is influenced by the curved surface and is similar to the initial curved surface profile.

The use of L-dopa induces the resistance of Mauthner neurons to the neurotoxic action of beta-amyloid

BACKGROUND: Mauthner goldfish (Carassius auratus (L)) cells serve as model objects for studying brain pathologies at the level of identified neurons and their individual dendrites. L-dopa may be an agent that decelerates the destruction of neurons caused by the toxic effects of beta-amyloid.
 AIMS: To study the three-dimensional structure of Mauthner neurons in goldfish and the ultrastructure of their afferent synapses under the influence of L-dopa and the toxic 2535 fragment of beta-amyloid.
 MATERIAL AND METHODS: This study was performed on the Mauthner neurons of goldfish fry (n=12) by using light and electron microscopy. Serial sections 3 m thick were used to identify and integrally reconstruct the structure of Mauthner neurons; determine the volume of the soma and ventral and lateral dendrites; and study the structure of afferent synapses.
 RESULTS: The use of L-dopa stabilized the size of the soma and ventral dendrites. The reduction in the volume of lateral dendrites was accompanied either by an increase in the volume of their branches under the action of beta-amyloid followed by that of L-dopa or by an increase in the volume of medial dendrites under the action of L-dopa followed by that of beta-amyloid. Although pathological changes in the ultrastructure of neurons and afferent synapses were not found, signs of early amyloidosis were detected.
 CONCLUSION: The use of L-dopa decelerates the degeneration of Mauthner neurons. The resistance of whole neurons to the neurotoxic action of beta-amyloid has been suggested to be due to the mechanism of structural homeostasis aiming at the compensatory restoration of the morphological organization of neurons.

Differential Subcellular Distribution and Release Dynamics of Cotransmitted Cholinergic and GABAergic Synaptic Inputs Modify Dopaminergic Neuronal Excitability.

We identified three types of monosynaptic cholinergic inputs spatially arranged onto medial substantia nigra dopaminergic neurons in male and female mice: cotransmitted acetylcholine (ACh)/GABA, GABA-only, and ACh only. There was a predominant GABA-only conductance along lateral dendrites and soma-centered ACh/GABA cotransmission. In response to repeated stimulation, the GABA conductance found on lateral dendrites decremented less than the proximally located GABA conductance, and was more effective at inhibiting action potentials. While soma-localized ACh/GABA cotransmission showed depression of the GABA component with repeated stimulation, ACh-mediated nicotinic responses were largely maintained. We investigated whether this differential change in inhibitory/excitatory inputs leads to altered neuronal excitability. We found that a depolarizing current or glutamate preceded by cotransmitted ACh/GABA was more effective in eliciting an action potential compared with current, glutamate, or ACh/GABA alone. This enhanced excitability was abolished with nicotinic receptor inhibitors, and modulated by T- and L-type calcium channels, thus establishing that activity of multiple classes of ion channels integrates to shape neuronal excitability.SIGNIFICANCE STATEMENT Our laboratory has previously discovered a population of substantia nigra dopaminegic neurons (DA) that receive cotransmitted ACh and GABA. This study used subcellular optogenetic stimulation of cholinergic presynaptic terminals to map the functional ACh and GABA synaptic inputs across the somatodendritic extent of substantia nigra DA neurons. We determined spatially clustered GABA-only inputs on the lateral dendrites while cotransmitted ACh and GABA clustered close to the soma. We have shown that the action of GABA and ACh in cotransmission spatially clustered near the soma play a critical role in enhancing glutamate-mediated neuronal excitability through the activation of T- and L-type voltage-gated calcium channels.

Pathological consequences of chronic olfactory inflammation on neurite morphology of olfactory bulb projection neurons

Chronic olfactory inflammation (COI) in conditions such as chronic rhinosinusitis significantly impairs the functional and anatomical components of the olfactory system. COI induced by intranasal administration of lipopolysaccharide (LPS) results in atrophy, gliosis, and pro-inflammatory cytokine production in the olfactory bulb (OB). Although chronic rhinosinusitis patients have smaller OBs, the consequences of olfactory inflammation on OB neurons are largely unknown. In this study, we investigated the neurological consequences of COI on OB projection neurons, mitral cells (MCs) and tufted cells (TCs). To induce COI, we performed unilateral intranasal administration of LPS to mice for 4 and 10 weeks. Effects of COI on the OB were examined using RNA-sequencing approaches and immunohistochemical analyses. We found that repeated LPS administration upregulated immune-related biological pathways in the OB after 4 weeks. We also determined that the length of TC lateral dendrites in the OB significantly decreased after 10 weeks of COI. The axon initial segment of TCs decreased in number and in length after 10 weeks of COI. The lateral dendrites and axon initial segments of MCs, however, were largely unaffected. In addition, dendritic arborization and AIS reconstruction both took place following a 10-week recovery period. Our findings suggest that olfactory inflammation specifically affects TCs and their integrated circuitry, whereas MCs are potentially protected from this condition. This data demonstrates unique characteristics of the OBs ability to undergo neuroplastic changes in response to stress.

A fluorinated SEI layer induced by a fire-retardant gel polymer electrolyte boosting lateral dendrite growth

A flame-retardant electrolyte fabricated by the in situ polymerization method facilitates lateral Li dendrite growth induced by a LiF-rich SEI layer.

Subcellular Dissection of a Simple Neural Circuit: Functional Domains of the Mauthner-Cell During Habituation.

Sensorimotor integration is a pivotal feature of the nervous system for ensuring a coordinated motor response to external stimuli. In essence, such neural circuits can optimize behavioral performance based on the saliency of environmental cues. In zebrafish, habituation of the acoustic startle response (ASR) is a simple behavior integrated into the startle command neurons, called the Mauthner cells. Whereas the essential neuronal components that regulate the startle response have been identified, the principles of how this regulation is integrated at the subcellular regions of the Mauthner cell, which in turn modulate the performance of the behavior, is still not well understood. Here, we reveal mechanistically distinct dynamics of excitatory inputs converging onto the lateral dendrite (LD) and axon initial segment (AIS) of the Mauthner cell by in vivo imaging glutamate release using iGluSnFR, an ultrafast glutamate sensing fluorescent reporter. We find that modulation of glutamate release is dependent on NMDA receptor activity exclusively at the AIS, which is responsible for setting the sensitivity of the startle reflex and inducing a depression of synaptic activity during habituation. In contrast, glutamate-release at the LD is not regulated by NMDA receptors and serves as a baseline component of Mauthner cell activation. Finally, using in vivo calcium imaging at the feed-forward interneuron population component of the startle circuit, we reveal that these cells indeed play pivotal roles in both setting the startle threshold and habituation by modulating the AIS of the Mauthner cell. These results indicate that a command neuron may have several functionally distinct regions to regulate complex aspects of behavior.

Heterogeneity of the Dendrite Array Created in the Root of Cored SX Turbine Blades during Initial Stage of Crystallization.

The roots of cored single-crystalline turbine blades made of a nickel-based CMSX-4 superalloy were studied. The casts were solidified by the vertical Bridgman method in an industrial ALD furnace using the spiral selector and selector continuer situated asymmetrically in the blade root transverse section. Scanning electron microscopy, the Laue diffraction and X-ray diffraction topography were used to visualize the dendrite array and the local crystal misorientation of the roots. It has been stated that heterogeneity of the dendrite array and creation of low-angle boundaries (LABs) are mostly related to the lateral dendrite branching and rapid growth of the secondary and tertiary dendrites near the surface of the continuer–root connection. These processes have an unsteady character. Additionally, the influence of the mould walls on the dendrite array heterogeneity was studied. The processes of the lateral growth of the secondary dendrites and competitive longitudinal growth of the tertiary dendrites are discussed and a method of reducing the heterogeneity of the root dendrite array is proposed.

Topographically Distinct Projection Patterns of Early-Generated and Late-Generated Projection Neurons in the Mouse Olfactory Bulb.

In the mouse brain, olfactory information is transmitted to the olfactory cortex via olfactory bulb (OB) projection neurons known as mitral and tufted cells. Although mitral and tufted cells share many cellular characteristics, these cell types are distinct in their somata location and in their axonal and dendritic projection patterns. Moreover, mitral cells consist of heterogeneous subpopulations. We have previously shown that mitral cells generated at different embryonic days differentially localize within the mitral cell layer (MCL) and extend their lateral dendrites to different sublayers of the external plexiform layer (EPL). Here, we examined the axonal projection patterns from the subpopulations of OB projection neurons that are determined by the timing of neurogenesis (neuronal birthdate) to understand the developmental origin of the diversity in olfactory pathways. We separately labeled early-generated and late-generated OB projection neurons using in utero electroporation performed at embryonic day (E)11 and E12, respectively, and quantitatively analyzed their axonal projection patterns in the whole mouse brain using high-resolution 3D imaging. In this study, we demonstrate that the axonal projection of late-generated OB projection neurons is restricted to the anterior portion of the olfactory cortex while those of the early-generated OB projection neurons innervate the entire olfactory cortex. Our results suggest that the late-generated mitral cells do not extend their axons to the posterior regions of the olfactory cortex. Therefore, the mitral cells having different birthdates differ, not only in cell body location and dendritic projections within the OB, but also in their axonal projection pattern to the olfactory cortex.

Evolutionary and homeostatic changes in morphology of visual dendrites of Mauthner cells in Astyanax blind cavefish.

Mauthner cells are the largest neurons in the hindbrain of teleost fish and most amphibians. Each cell has two major dendrites thought to receive segregated streams of sensory input: the lateral dendrite receives mechanosensory input while the ventral dendrite receives visual input. These inputs, which mediate escape responses to sudden stimuli, may be modulated by the availability of sensory information to the animal. To understand the impact of the absence of visual information on the morphologies of Mauthner cells during developmental and evolutionary time scales, we examined the teleost Astyanax mexicanus. This species of tetra is found in two morphs: a seeing surface fish and a blind cavefish. We compared the structure of Mauthner cells in surface fish raised under daily light conditions, in surface fish raised in constant darkness, and in two independent lineages of cave populations. The length of ventral dendrites of Mauthner cells in dark-raised surface fish larvae were longer and more branched, while in both cave morphs the ventral dendrites were smaller or absent. The absence of visual input in surface fish with normal eye development leads to a homeostatic increase in dendrite size, whereas over evolution, the absence of light led to the loss of eyes and a reduction in dendrite size.

CCKergic Tufted Cells Differentially Drive Two Anatomically Segregated Inhibitory Circuits in the Mouse Olfactory Bulb.

Delineation of functional synaptic connections is fundamental to understanding sensory processing. Olfactory signals are synaptically processed initially in the olfactory bulb (OB) where neural circuits are formed among inhibitory interneurons and the output neurons mitral cells (MCs) and tufted cells (TCs). TCs function in parallel with but differently from MCs and are further classified into multiple subpopulations based on their anatomic and functional heterogeneities. Here, we combined optogenetics with electrophysiology to characterize the synaptic transmission from a subpopulation of TCs, which exclusively express the neuropeptide cholecystokinin (CCK), to two groups of spatially segregated GABAergic interneurons, granule cells (GCs) and glomerular interneurons in mice of both sexes with four major findings. First, CCKergic TCs receive direct input from the olfactory sensory neurons (OSNs). This monosynaptic transmission exhibits high fidelity in response to repetitive OSN input. Second, CCKergic TCs drive GCs through two functionally distinct types of monosynaptic connections: (1) dendrodendritic synapses onto GC distal dendrites via their lateral dendrites in the superficial external plexiform layer (EPL); (2) axodendritic synapses onto GC proximal dendrites via their axon collaterals or terminals in the internal plexiform layer (IPL) on both sides of each bulb. Third, CCKergic TCs monosynaptically excite two subpopulations of inhibitory glomerular interneurons via dendrodendritic synapses. Finally, sniff-like patterned activation of CCKergic TCs induces robust frequency-dependent depression of the dendrodendritic synapses but facilitation of the axodendritic synapses. These results demonstrated important roles of the CCKergic TCs in olfactory processing by orchestrating OB inhibitory activities.SIGNIFICANCE STATEMENT Neuronal morphology and organization in the olfactory bulb (OB) have been extensively studied, however, the functional operation of neuronal interactions is not fully understood. We combined optogenetic and electrophysiological approaches to investigate the functional operation of synaptic connections between a specific population of excitatory output neuron and inhibitory interneurons in the OB. We found that these output neurons formed distinct types of synapses with two populations of spatially segregated interneurons. The functional characteristics of these synapses vary significantly depending on the presynaptic compartments so that these output neurons can dynamically rebalance inhibitory feedback or feedforward to other neurons types in the OB in response to dynamic rhythmic inputs.

In situformation of a Li–Sn alloy protected layer for inducing lateral growth of dendrites

A SnO2modified separator prepared by magnetron sputtering guides lateral Li dendrite growth in lithium metal batteries.

Vasopressin Cells in the Rodent Olfactory Bulb Resemble Non-Bursting Superficial Tufted Cells and Are Primarily Inhibited upon Olfactory Nerve Stimulation.

The intrinsic vasopressin system of the olfactory bulb is involved in social odor processing and consists of glutamatergic vasopressin cells (VPCs) located at the medial border of the glomerular layer. To characterize VPCs in detail, we combined various electrophysiological, neuroanatomical, and two-photon Ca2+ imaging techniques in acute bulb slices from juvenile transgenic rats with eGFP-labeled VPCs. VPCs showed regular non-bursting firing patterns, and displayed slower membrane time constants and higher input resistances versus other glutamatergic tufted cell types. VPC axons spread deeply into the external plexiform and superficial granule cell layer (GCL). Axonal projections fell into two subclasses, with either denser local columnar collaterals or longer-ranging single projections running laterally within the internal plexiform layer and deeper within the granule cell layer. VPCs always featured lateral dendrites and a tortuous apical dendrite that innervated a single glomerulus with a homogenously branching tuft. These tufts lacked Ca2+ transients in response to single somatically-evoked action potentials and showed a moderate Ca2+ increase upon prolonged action potential trains.Notably, electrical olfactory nerve stimulation did not result in synaptic excitation of VPCs, but triggered substantial GABAA receptor-mediated IPSPs that masked excitatory barrages with yet longer latency. Exogenous vasopressin application reduced those IPSPs, as well as olfactory nerve-evoked EPSPs recorded from external tufted cells. In summary, VPCs can be classified as non-bursting, vertical superficial tufted cells. Moreover, our findings imply that sensory input alone cannot trigger excitation of VPCs, arguing for specific additional pathways for excitation or disinhibition in social contexts.

Regulation of subcellular dendritic synapse specificity by axon guidance cues.

Neural circuit assembly occurs with subcellular precision, yet the mechanisms underlying this precision remain largely unknown. Subcellular synaptic specificity could be achieved by molecularly distinct subcellular domains that locally regulate synapse formation, or by axon guidance cues restricting access to one of several acceptable targets. We address these models using two Drosophila neurons: the dbd sensory neuron and the A08a interneuron. In wild-type larvae, dbd synapses with the A08a medial dendrite but not the A08a lateral dendrite. dbd-specific overexpression of the guidance receptors Unc-5 or Robo-2 results in lateralization of the dbd axon, which forms anatomical and functional monosynaptic connections with the A08a lateral dendrite. We conclude that axon guidance cues, not molecularly distinct dendritic arbors, are a major determinant of dbd-A08a subcellular synapse specificity.

Basal forebrain GABAergic innervation of olfactory bulb periglomerular interneurons.

Basal forebrain long-range projections to the olfactory bulb are important for olfactory sensitivity and odour discrimination. Using optogenetics, it was confirmed that basal forebrain afferents mediate IPSCs on granule and deep short axon cells. It was also shown that they selectively innervate specific subtypes of periglomerular (PG) cells. Three different subtypes of type 2 PG cells receive GABAergic IPSCs from the basal forebrain but not from other PG cells. Type 1 PG cells, in contrast, do not receive inputs from the basal forebrain but do receive inhibition from other PG cells. These results shed new light on the complexity and specificity of glomerular inhibitory circuits, as well as on their modulation by the basal forebrain. Olfactory bulb circuits are dominated by multiple inhibitory pathways that finely tune the activity of mitral and tufted cells, the principal neurons, and regulate odour discrimination. Granule cells mediate interglomerular lateral inhibition between mitral and tufted cells' lateral dendrites whereas diverse subtypes of periglomerular (PG) cells mediate intraglomerular lateral inhibition between their apical dendrites. Deep short axon cells form broad intrabulbar inhibitory circuits that regulate both populations of interneurons. Little is known about the extrabulbar GABAergic circuits that control the activity of these various interneurons. We examined this question using patch-clamp recordings and optogenetics in olfactory bulb slices from transgenic mice. We showed that axonal projections emanating from diverse basal forebrain GABAergic neurons densely project in all layers of the olfactory bulb. These long-range GABAergic projections provide a prominent synaptic input on granule and short axon cells in deep layers as well as on selective subtypes of PG cells. Specifically, three different subclasses of type 2 PG cells receive robust and target-specific basal forebrain inputs but have little local interactions with other PG cells. In contrast, type 1 PG cells are not innervated by basal forebrain fibres but do interact with other PG cells. Thus, attention-regulated basal forebrain inputs regulate inhibition in all layers of the olfactory bulb with a previously overlooked synaptic complexity that further defines interneuron subclasses.

Self-Supporting Dendritic Copper Porous Film Inducing the Lateral Growth of Metallic Lithium for Highly Stable Li Metal Battery

Lithium metal is recognized as the most promising anode candidate for advanced high-energy-density batteries. However, uncontrolled vertical growth of Li dendrites will induce short-circuit and cause rapid capacity loss, which hinders the practical application of rechargeable Li-metal batteries. The regulation of Li dendrites from vertical to lateral growth may be an effective strategy to solve the problems. Herein, a self-supporting dendritic copper porous film as host matrix of lithium is constructed by a one-step facile and cost-effective electrodeposition on gas-liquid-solid interface to inhibit the vertical growth of Li dendrites. Li nucleates on the surface of copper nanoparticles and grows laterally by the induced action of lateral copper dendrites consisting of nanoparticles. Meanwhile, copper porous film with dendritic walls provides more nucleation sites for uniform Li deposition and space for Li storage. On this basis, high and stable Coulombic efficiency of 98% after 600 cycles at 1 mA cm−2 and prolong lifespan of 2400 h in the symmetric cell are achieved. Coupled with a LiFePO4 cathode, the full cell exhibits good rate performance and cycle stability with capacity retention of 82.4% for 250 cycles at 1 C. It provides a simple but effective way to realize a stable Li metal-based anode.

Changes in the Dendrite Morphology of Mauthner Neurons in Goldfish under the Conditions of Monocular Deprivation and Sensory Stimulation

Abstract—The effects of sensory stimulation on the model of paired Mauthner neurons of monocularly deprived goldfish have been studied by light- and electron microscopy. Three-dimensional reconstruction was performed using serial semi-thin sections in order to determine the volumes of soma and dendrites for the right and left Mauthner neurons. These data suggested that long-term stimulation of some fish caused a decrease in the volume of the lateral dendrites of neurons contralateral with respect to the side of the enucleated eye. As a result, the morphological homeostasis in the dendrites of Mauthner neurons, which was observed in the control fish, was disturbed after the effect of stimulation upon this group of fish. The main mechanism of this effect of stimulation, according to ultrastructural analysis, is the compaction of the cytoskeleton in the lateral dendrite of a neuron and a reduction in the number of synaptic vesicles in the region of afferent synapses. In contrast, stimulation of the remaining fish induced the resistance of the contralateral Mauthner neuron to prolonged stimulation, as well as the hypertrophy of its lateral and shortening of the ventral dendrites. These results suggest that the homeostasis of the dendrites of Mauthner neurons essentially depends on the state of the visual inputs, the load on which increased as a result of the doubled impact.

Quantitative Association of Anatomical and Functional Classes of Olfactory Bulb Neurons.

Juxtaglomerular cells (JGCs) of the olfactory bulb (OB) glomerular layer (GL) play a fundamental role in olfactory information processing. Their variability in morphology, physiology, and connectivity suggests distinct functions. The quantitative understanding of population-wise morphological and physiological properties and a comprehensive classification based on quantitative parameters, however, is still lacking, impeding the analysis of microcircuits. Here, we provide multivariate clustering of 95 in vitro sampled cells from the GL of the mouse (male or female C57BL/6) OB and perform detailed morphological and physiological characterization for the seven computed JGC types. Using a classifier based on a subselection of parameters, we identified the neuron types in paired recordings to characterize their functional connectivity. We found that 4 of the 7 clusters comply with prevailing concepts of GL cell types, whereas the other 3 represent own distinct entities. We have labeled these entities horizontal superficial tufted cell (hSTC), vertical superficial tufted cell, and microglomerular cell (MGC): The hSTC is a tufted cell with a lateral dendrite that much like mitral cells and tufted cells receives excitatory inputs from the external tufted cell but likewise serves as an excitatory element for glomerular interneurons. The vertical superficial tufted cell, on the other hand, represents a tufted cell type with vertically projecting basal dendrites. We further define the MGC, characterized by a small dendritic tree and plateau action potentials. In addition to olfactory nerve-driven and external tufted cell driven interneurons, these MGCs represent a third functionally distinct type, the hSTC-driven interneurons. The presented correlative analysis helps to bridge the gap between branching patterns and cellular functional properties, permitting the integration of results from in vivo recordings, advanced morphological tools, and connectomics.SIGNIFICANCE STATEMENT The variance of neuron properties is a feature across mammalian cerebral circuits, contributing to signal processing and adding computational robustness to the networks. It is particularly noticeable in the glomerular layer of the olfactory bulb, the first site of olfactory information processing. We provide the first unbiased population-wise multivariate analysis to correlate morphological and physiological parameters of juxtaglomerular cells. We identify seven cell types, including four previously described neuron types, and identify further three distinct classes. The presented correlative analysis of morphological and physiological parameters gives an opportunity to predict morphological classes from physiological measurements or the functional properties of neurons from morphology and opens the way to integrate results from in vivo recordings, advanced morphological tools, and connectomics.

Solid-Liquid Electrolyte as a Nanoion Modulator for Dendrite-Free Lithium Anodes.

Rechargeable lithium (Li) metal batteries are considered the most promising of Li-based energy storage technologies. However, tree-like dendrite produced by irregular Li+ electrodeposition restricts it wide applications. Herein, based on a cation-microphase-regulation strategy, we create solid-liquid electrolytes (SLEs) by absorbing commercial liquid electrolytes into polyethylene glycol (PEG) engineered nanoporous Al2O3 ceramic membranes. By means of molecular dynamics simulations and comprehensive experiments, we show that Li ions are regulated and promoted in the two microphases, the channel phase and nonchannel phase, respectively. The channel phase can achieve homogeneous Li+ flux distribution by multiple mechanisms, including its uniform array of nanochannels and ability to suppress lateral dendrite growth by its high modulus. In the nonchannel phase, PEG chains swollen by electrolyte facilitate desolvation and fast conduction of Li+. As a result, the studied SLEs exhibit high ionic conductivity, low interfacial resistance, and the unique ability to stabilize deposition at the Li anode. By means of galvanostatic cycling studies in symmetric Li cells and Li/Li4Ti5O12 cells, we further show that the materials open a path to Li metal batteries with excellent cycling performance.

Cobalt electrowinning – A systematic investigation for high quality electrolytic cobalt production

The process of Minera y Metalúrgica del Boleo recovers cobalt from a complex oxide and sulfide ore by solvent extraction and electrowinning after the recovery of copper and zinc (as copper cathode and zinc sulfate monohydrate). As part of the process development, cobalt electrowinning was systematically studied with respect to temperature, pH, pitting behavior, deposit morphology, deposition stress and interfacial tension. Electrowinning test work was conducted in a bench scale diaphragm cell in a sulfate medium with control of the catholyte pH. Cobalt was deposited on a coated stainless-steel mandrel cathode with exposed circles to allow plating of disk shaped deposits over the surface of the cathode that could be easily stripped after 5–7 days of deposition. Limiting the area of plating prevents the detachment of the cobalt deposit from the cathode due to high deposition stress. The morphology and pitting behaviour varied for different commercial cobalt raw materials used for making cobalt sulfate solution as they contained different impurities or additives. The deposition behaviour changed after the solutions had an activation carbon treatment as some impurities (mainly organic compounds) were removed. In the temperature range from 50 to 65 °C, a higher temperature resulted in a brighter deposit with more pits and smaller lateral dendrites. The cobalt deposition current efficiency was around 98% at pH 3.0 and was not sensitive to temperature. With decreasing catholyte pH from 3.5 to 2.0, the cobalt current efficiency decreased from 99 to 88% and the growth of lateral dendrites were significantly suppressed. The formation of pits resulted from the adhesion of hydrogen bubbles on the cobalt deposit. The adhesion of hydrogen bubbles was determined by the prevailing surface and interfacial tensions. Anti-pitting agents were used to change the interfacial and surface tensions in such a way that the detachment of bubbles was facilitated, resulting in a pit-free cobalt deposit. The anti-pitting agents affected the deposit morphology and internal stress. The impact of the anti-pitting agents on the disengagement of organic and aqueous phases during cobalt solvent extraction was also investigated. The internal stress of the cobalt deposit as a function of temperature and anti-pitting agent dosage and the conductivities of the CoSO4-Na2SO4-H2SO4 system are reported.