Changes in the Dendrite Morphology of Mauthner Neurons in Goldfish under the Conditions of Monocular Deprivation and Sensory Stimulation

Abstract

Abstract—The effects of sensory stimulation on the model of paired Mauthner neurons of monocularly deprived goldfish have been studied by light- and electron microscopy. Three-dimensional reconstruction was performed using serial semi-thin sections in order to determine the volumes of soma and dendrites for the right and left Mauthner neurons. These data suggested that long-term stimulation of some fish caused a decrease in the volume of the lateral dendrites of neurons contralateral with respect to the side of the enucleated eye. As a result, the morphological homeostasis in the dendrites of Mauthner neurons, which was observed in the control fish, was disturbed after the effect of stimulation upon this group of fish. The main mechanism of this effect of stimulation, according to ultrastructural analysis, is the compaction of the cytoskeleton in the lateral dendrite of a neuron and a reduction in the number of synaptic vesicles in the region of afferent synapses. In contrast, stimulation of the remaining fish induced the resistance of the contralateral Mauthner neuron to prolonged stimulation, as well as the hypertrophy of its lateral and shortening of the ventral dendrites. These results suggest that the homeostasis of the dendrites of Mauthner neurons essentially depends on the state of the visual inputs, the load on which increased as a result of the doubled impact.