Late Miocene Research Articles

Post-cranial remains of Rhinocerotidae from the Neogene of central Myanmar: morphological descriptions and comparisons with ratios

ABSTRACT New post-cranial remains from the early Late Miocene have been discovered in the lowermost part of the Irrawaddy Formation, Tebingan area, central Myanmar. Three genera and one indeterminate taxon were identified: Rhinoceros sp. Rhinoceros cf. R. sondaicus, Dicerorhinus sp., Brachypotherium perimense, and Rhinocerotidae indet. The evolutionary history of the Rhinocerotidae is still poorly known in Southeast Asia. Few Rhinoceros species, Rhinoceros sp. ‘B’. fatehjangense, and B. perimense, has already been identified in the Tebingan area by dental remains. The present discovery of the post-cranial remains of Rhinoceros cf. R. sondaicus and Dicerorhinus sp. that complements previous studies based on dental remains. The early Late Miocene Tebingan record is the oldest fossil record of Dicerorhinus in Southeast Asia, indicating a continental origin of the genus. Furthermore, the possible presence of R. sondaicus in the Tebingan area suggests that it may have appeared in Southeast Asia as early as the early Late Miocene.

Four in one: An integrative taxonomic revision of the Microhyla berdmorei complex (Amphibia: Anura: Microhylidae) illustrates the tremendous amphibian diversity of Southeast Asia

Abstract Berdmore’s narrow-mouthed frog, Microhyla berdmorei (Blyth, 1856), is the largest member of the genus Microhyla and is distributed all over Southeast Asia, from Northeast India and southern China to Sumatra and Borneo in Indonesia. Here we demonstrate that M. berdmorei represents a complex of four species that are morphologically, acoustically, and genetically distinct from each other, and we implement taxonomic revisions. Phylogenetic analyses of three mitochondrial DNA (hereafter mtDNA, including COI, 12S, and 16S rRNA; 3119 bp) and one nuclear (BDNF; 716 bp) gene sequences are corroborated by phylogenomic analyses of 2700 ddRAD-seq loci (387,270 bp). All support that the M. berdmorei complex, which originates from the early Miocene (ca. 19.7 mya), consists of two clades that we date to the late Miocene (ca. 7.5 mya). The first clade, which regroups populations of large-sized individuals, is distributed in Indo-Burma and includes a lineage from Northeast India, Bangladesh, and Myanmar (M. berdmorei sensu stricto) and a lineage from West Malaysia, Thailand, Laos, Cambodia, and Vietnam (for which we propose to resurrect the species M. malcolmi Cochran, 1927). The second clade, which regroups populations of small-sized individuals, occurs in Sundaland and also includes two lineages for which we provide taxonomic descriptions on species rank. The first new species is widely distributed from Peninsular Malaysia to the islands of Borneo and Sumatra (described herein as M. sundaicasp. nov.), while the second one is restricted to the Malay Peninsula and occurs in extreme southern Thailand and adjacent Malaysia (M. peninsularissp. nov.). We further provide evidence for the synonymy of Callula natatrix Cope, 1867 with M. berdmorei sensu stricto, and M. fowleri Taylor, 1934 with M. malcolmi. Our study illustrates the high diversity of Southeast Asian amphibians, especially in the genus Microhyla, which presently totals 54 species.

Pulsed uplift of the South Tianshan since the Late Miocene indicated by the linear inversion on river longitudinal profiles

The early Cenozoic collision and subsequent continuous convergence between the Indian and Eurasian plates reactivated the Tianshan orogen in the Central Asia and caused multistage uplift of the mountain range. The modern Tianshan, with high mountain peaks of >7000 m, forms very prominent topography that profoundly affects the regional tectonics and climate. However, the late Cenozoic uplift process and topography growth of the South Tianshan remain controversial. River longitudinal profile inversion provides a distinctive way to reveal rock uplift history since the late Cenozoic. In this study, we presented linear inversion on river longitudinal profiles of four drainage basins originating from high mountains in the South Tianshan. The inversion results from two basins in the northern flank show fast and continuous increases in the uplift rates from about 0.1–0.2 mm/a to 0.6–1.0 mm/a since 4 Ma. While in the southern flank, the uplift rates of the two basins increased gradually from about 0.1 mm/a to 0.2 mm/a before 10 Ma and from 0.2 mm/a to 0.4 mm/a around 10 Ma and 6 Ma, respectively. Our results combined with recently published chronological data in this region indicate that the South Tianshan experienced a pulsed tectonic uplift process since the Late Miocene. Moreover, the pulsed tectonic uplift should lead to an elevated South Tianshan during 6–4 Ma, coinciding well with the ∼5.3 Ma extreme aridification in the Tarim Basin, thus supporting that the rain shadow effect caused by high topography of South Tianshan is a critical reason for aridification.

Ceratophryid frogs in the late Miocene of central Andes of Argentina: insights on the paleoenvironment of Palo Pintado Formation

ABSTRACT Ceratophryid frogs inhabit the lowlands of South America and exhibit greater diversity in the semiarid Gran Chaco. The presence of Ceratophryidae dates back to the late Miocene on the eastern slopes of the Andes in Argentina. Herein, we describe the remains of Ceratophrys from late Miocene of Palo Pintado Formation (Salta, Argentina). Fragmentary bones were recovered from the base of the unit in beds dated between 8 and 7.7 million years ago. The specimen IBIGEO-P141 exhibits the fusion of dentary and mentomeckelian bones forming a fang, a sharp-edged lower jaw, and sculptured bones as Ceratophryidae. The granular pattern of sculpturation, differences between pars facialis and pars dentalis of maxillary, and the shape of lower jaw, support its assignment to the genus Ceratophrys. Combined bioclimatic data and geographic distribution of living Ceratophrys indicate a strong influence of precipitation variables in the distribution of these frogs with adaptations to survive long dry periods. These facts suggest a seasonal rainfall with a well-defined dry season during the deposition of lower levels of Palo Pintado Formation. The palaeoclimatic conditions resemble those occurring in the actual semiarid Chaco, and reveal that the historical geographic distribution of Ceratophryidae was retracted eastward due to the Andean uplift.

A link of the Late Miocene giraffid migration pathway from the peri-Aegean lands to the northeastern Eurasian areas

The southeastern retreat of the Paratethys Sea constrained by the Middle Sarmatian uplift of the last Eastern Carpathians nappe (Pericarpathian Nappe) caused the progressive emergence of land masses contiguous to the Dacian Basin. Diverse faunas, including large mammals, colonized these lands. However, giraffes remain poorly documented. This paper attests to the presence of giraffids documented by postcranial bones of Samotherium major and Helladotherium duvernoyi. So far, both genera have only been listed in Romania without any information about the retrieved fossil bones, description, or illustration. The fossils described herein – a metacarpal of Samotherium major and two metatarsals, and an astragalus of Helladotherium duvernoyi – were sampled from four Late Miocene outcrops of the Eastern Carpathians Foreland (Crețești 1, Văleni, Pogana and Fălciu). Two outcrops (Văleni and Fălciu) have been newly discovered. This paper also describes for the first time Samotherium major in Romania. During the Late Miocene, the giraffids were elemental components of the so-called Pikermian Biome of the Greco-Irano-Afghan Province. This biome extended from the peri-Aegean lands to Iran. However, several occurrences are known from northeastern Eurasian areas (Ukraine, Republic of Moldova, and Hungary). The new Romanian occurrences represent a link between the southern and northeastern giraffid faunas during the Late Miocene, indicating a presumed migration pathway.

Using detrital zircon U[sbnd]Pb geochronology to track the provenance of Miocene sediments in the northern Pearl River Mouth Basin, South China Sea

The Late Cenozoic provenance study of the Pearl River Mouth Basin (PRMB) holds significant implications for understanding the tectonic-climatic interactions, drainage evolution, and oceanic circulation in the northern South China Sea. However, the Miocene sediment routing system remains poorly understood due to the paucity of zircon geochronological data and the effects of sediment mixing and homogenization. This study reconstructs the ancient source-to-sink system by means of detrital zircon analyses from the northern PRMB and zircon age-based mixture modeling of well-defined provenance end-members. The results disclose varied and complex source-to-sink scenarios since the Early Miocene, involving three primary contributors: the Pearl River drainage system, coastal SE China, and Taiwan Island. In combination with seismic facies and climatic proxies, the intensive addition of Precambrian zircons suggests that, during the Middle and Late Miocene, progressive drainage expansion within the Yangtze Block likely contributed to the increasing sediment supply from the western Pearl River drainage system. Furthermore, the provenance shift in the northern PRMB, as indicated by the Mesozoic ages, may reflect a response to the activation of oceanic circulation and intensification of the East Asian monsoon. Additionally, though less significant, contributions from the Hainan and Luzon Island are also observed, indicating the input of the branch of the Kuroshio Current during the Late Miocene.

New bulk rock and age data on the changes in magma evolution during the Miocene, Galatean volcanic area, central Anatolia, Turkey: A review

The Galatean Volcanic Province (GVP) lies within the Sakarya tectonic belt in the northwest of central Anatolia, Turkey, south of the North Anatolian Fault, cropping out over 12,000 km2. It consists of Early Miocene intermediate to acid lavas, pyroclastic rocks, volcaniclastic deposits, and Late Miocene OIB-like basalts. Our study is based on new bulk geochemistry, SrNd isotopes, and 40Ar/39Ar dating of the volcanic rocks from the south-western part of GVP, but integrates all the available previous data to understand how magmas evolved in the post-collisional geodynamic condition in the GVP. The initial eruptions were rhyolitic, as domes or pyroclastic deposits (Group 1) and basaltic lavas (Group 2) during the Early Miocene. Group 2 is also represented by large volumes of basaltic-andesitic, trachyandesite-dacite/trachydacite lava flows, small intrusions, and pyroclastic deposits as generated between 21 and 14 Ma. Relatively low 87Sr/86Sr ratios (0.705–0.706) of the early rhyolites (Group 1), similar to all the rocks from Group (2), suggest the generation of hybrid melts with variable contributions of mantle-derived and crustal material. The volcanic activity ends with OIB-like basalts (11–7 Ma) showing the lowest 87Sr/86Sr (∼0.703) suggesting an asthenospheric origin.The geodynamic model, based on post-Cyprian slab rollback, results in long-term (22–13 Ma) post-collisional delamination/drip processes that support magmatism. This magmatism is generated in the lithospheric mantle, with the formation of basaltic melts and acid hybrid melts showing variable contributions of mantle-derived and crustal materials in a complex trans-crustal magma plumbing system. Complex mixing of various intra-crustal magmas and fractional crystallization processes generated a huge volume of volcanic rocks. The Late Miocene small-volume OIB basalts were asthenospheric melts that during the late stage recorded localized decompression melting processes.

Molybdenite Re-Os geochronology and conditions of formation of potassic and sodic-calcic alteration associated with the Plaka porphyry Mo-Cu system, Lavrion, Greece

The Plaka porphyry Mo-Cu system occurs in the world-class Lavrion Ag-Pb-Zn district in Attica, southern Greece. It is spatially associated with a granodiorite porphyry that intruded the Attic-Cycladic Crystalline Complex in the late Miocene, along the footwall of the Western Cycladic detachment fault. A Re-Os age of 9.51 ± 0.04 Ma indicates that molybdenite formed during the early stage of the granodiorite porphyry intrusion and that subsequent cooling was very rapid. Brittle deformation and hydrothermal fluid flow created a network of A-, B-, diopside-actinolite and D- veins, associated with potassic-, sodic-calcic- and sericitic alterations. Potassic alteration is characterized by secondary biotite + K-feldspar + quartz + magnetite ± apatite, contains disseminated molybdenite, pyrite, and chalcopyrite, and formed at 420–500 °C, at pressures up to 530 bars (< 5.3 km depth) from hydrothermal fluids that underwent phase separation. Sodic-calcic alteration is devoid of Cu-Mo mineralization and, consists of diopside + actinolite + oligoclase/andesine + titanite + magnetite ± epidote-allanite ± chlorite ± quartz, which corresponded to a temperature range of between 350 and < 500 °C. Primary magnetite, titanite and biotite crystallized between the nickel‑nickel oxide (NNO) and hematite-magnetite (HM) buffers, indicating fairly oxidizing conditions for the granodioritic magma. Hydrothermal biotite plots closer to the HM buffer suggesting increasing oxygen fugacity during exsolution of the hydrothermal fluids associated with potassic alteration. The system evolved toward more reducing conditions during sericitic alteration and associated pyrite-molybdenite mineralization. A combination of evaporated seawater and magmatic fluids likely caused formation of the sodic-calcic alteration through the decarbonation of the host marble.

New remains of the enormous hoplophorine glyptodont Uruguayurus Mones, 1987 (Mammalia, Cingulata) in a particular faunistic association, Raigón Formation (Pliocene–middle Pleistocene), Uruguay

A new specimen of the poorly known glyptodont Uruguayurus Mones, 1987, is described on the basis of a large portion of the caudal tube from Arazatí Harbor, San José Department, Uruguay. This fossil is attributed to the Raigón Formation (Pliocene–middle Pleistocene), which crops out in the coastal cliffs of the referenced locality. This unit shows a particular faunal association characterized by endemic species, other representatives of the South American Cenozoic and others of North American origin. The material described herein shows diagnostic characteristics that allow to consider it an hoplophorine glyptodont, pertaining to the genus referred to above, similar to and the probable descendant from the smaller Pseudoplohophorus Castellanos, 1926, which is abundant in deposits of the underlying Camacho Formation (late Miocene) at the same locality. On the basis of this finding, the knownledge of Uruguayurus is increased and its diagnosis expanded. The new specimen has a larger, flatter, and more symmetrical caudal tube than the specimens previously described, named U. authochthonus Mones1987, and U. lydekkeri (Mones, 1987). It is also distinguished from those morphotypes by having an interapical groove of intermedial width, very large apical and first lateral figures, and more abundant and clearly delimited peripheral figurines on the dorsal surface. For now, these differences could be attributed to intraspecific variations within Uruguayurus authochthonus.

Mitochondrial genome of Neuryurus rudis (Xenarthra, Cingulata); contribution to phylogeny and origin of glyptodonts

The remarkable glyptodonts have sparked the interest of evolutionary biologists since the 19th century, in their attempts to elucidate the phylogenetic relationships among the various species of these armored giants and their relationship with other xenarthrans. In recent years, the molecular analysis of the first glyptodont has included them within the cingulates, as a special group of armadillos that lost the mobility of the bands of their armor during their evolutionary history. In this research, we obtained the mitochondrial DNA sequence of the elusive and poorly known glyptodont Neuryurus rudis, inferring its phylogenetic position with respect to the glyptodont Doedicurus sp. and extant armadillos. This study reaffirms glyptodonts as a subgroup of cingulates, with Neuryurus and Doedicurus sharing a common ancestor from the late Oligocene or early Miocene and traces the group’s origin back to an armadillo ancestor in the Eocene.

Miocene evolution of vegetation, climate, and elevation in the Wulan Basin of northeast Tibetan Plateau based on a CRACLE analysis of palynological assemblages

The uplift of the Tibetan Plateau (TP) during the Cenozoic has played a pivotal role in shaping global climate and vegetation evolution, however, understanding the uplift history of its various tectonic blocks remains complex. In this paper, we analyze palynological samples from the Miocene Xiayoushashan and Shangyoushashan formations in the Wulan Basin to explore the relationship between climate change and TP uplift. The analysis identified four distinct pollen zones: Zone I (18–15.3 Ma) characterized by Artemisia-Lycopodium-Aster-Polygonaceae; Zone II (15.3–12.7 Ma) dominated by Pinus-Picea-Aster-Artemisia; Zone III (12.7–11.7 Ma) featuring Lycopodium-Picea-Pinus-Polygonaceae; Zone IV (11.7–8.7 Ma) characterized by Picea-Chenopodiaceae-Lycopodium-Artemisia. Results indicate that the whole study interval was dominated by shrubs and grasses, interspersed with conifers and broad-leaved trees, exhibiting an altitudinally zonal pattern. Using the Coexistence Likelihood Estimation (CRACLE) method, we estimate a mean annual temperature (MAT) ranging from 6.0 to 14.7 °C and a mean annual precipitation (MAP) between 919.1 and 1612.4 mm during Miocene times. While minor climatic fluctuations occurred, the region experienced a cool-temperate and sub-humid climate significantly warmer and more humid conditions compared to the present. Based on MAT differences, the paleoelevation of the Wulan Basin during the Miocene was estimated to be c. 1655–2025 m, suggesting an uplift of c. 1275–1645 m since that time. These findings corroborate previous suggestions that the northeastern TP had not reached its current elevation prior to the Late Miocene.

The tibiotarsus of a giant darter from the upper Miocene of Amazonia and weight estimates for fossil darters

Fossil darters are known from every continent since the Oligocene. However, it was in the Miocene of South America that this group reached its maximum diversity. Macranhinga ranzii is the largest darter from the Solimões Formation, described based on the left and right femora as well as the distal end of a tarsometarsus and four vertebrae. Here, we describe for the first time the left tibiotarsus of this species by comparing it with the tibiotarsus of the extant Anhinga anhinga and other fossil anhingids. We also calculated and compared the body mass of Ma. ranzii to that of all known tibiotarsi fossil anhingids using two calculation methods. The tibiotarsus described here is morphologically similar to that of other anhingids, however, in addition to its large size and robustness, it presents diagnostic characters (e.g., torus linearis) that allow us to attribute it to the genus Macranhinga and differentiate it from other fossil species of this genus whose tibiotarsi are known. The estimated body mass of Ma. ranzii ranges from 14.39 to 19.1 kg, ranking it the highest estimated body mass among the darters where the tibiotarsi were found.

Paleogeographic implication of a titanosaur eggshell from the Neogene of the Entre Ríos Province, Argentina

The Lower Member of the Ituzaingó Formation (LMIF), fluvial in origin and assigned to the Upper Miocene, contains a conglomerate level known as “Mesopotamiense” or “Conglomerado osífero” that has yielded a rich fauna of vertebrates. The aim of this contribution is to describe a titanosaurian dinosaur eggshell fragment recovered from the LMIF at the locality of Toma Vieja (western Entre Ríos Province), discussing its paleogeographic history and implications. Fragments of eggshells referred to Titanosauria are frequent from litostratigraphic units of the Upper Cretaceous of western Uruguay (e.g., Guichón, Mercedes, and Queguay Formations). Besides, a fragment of eggshell recovered from the Puerto Yeruá Formation (Upper Cretaceous) at eastern Entre Ríos Province was described. Geophysical data from the Entre Ríos Province allow to propose the presence of a Lower Cretaceous field of extensional tectonic stresses that generated structural lineaments with a E-W and ENE-WSW trend. This ancient penetrative Cretaceous tectonic framework would have exerted control on the shape of the basin and sediment flow patterns and subsequent sedimentation, by promoting the generation of accommodation space over the late Paleogene and the Neogene. The phenomenon would have conditioned the deposition of the Fray Bentos Formation, the subsequent marine Paraná Formation, and the sedimentation of the LMIF, with source materials coming from the east. Thus, it is hypothesized that the fragment of eggshell of Titanosauria would come from the eastern area of the basin (western Uruguay) as a consequence of the existence of a paleohydric system with predominantly E-W and ENE-WSW directions. This late Neogene system would have been controlled by inherited Cretaceous structural lineaments with orientations similar to those that also govern the current fluvial systems of western Uruguay, developed on the Mesozoic substrate.

Mineralogy and Geochemistry of Upper Miocene Igneous Rocks, Kos Island, Greece: Extension during Strike-Slip Faulting and Subduction Rollback

Upper Miocene volcanic and plutonic rocks on Kos island preserve a record of magmatic and tectonic events in the transition zone between the Aegean and Anatolian microplates. Their field setting, syn-intrusion deformation, mineralogy, and geochemistry were investigated. Volcanic rocks, including trachyandesite flows and trachyandesite to rhyolite domes, were extruded on a central E–W horst and directly overlie Alpine basement. Thick successions of trachytic flow tuffs are interbedded with fluvial and lacustrine basinal sediments to the south of this horst. Volcanism was synchronous with the emplacement of the Dikeos monzonite pluton, which is geochemically similar to some lithic clasts in the thick flow tuffs and is cut by mafic dykes including lamprophyres. Two main types of mafic magma were present: a K-rich lamprophyric magma that evolved to trachyandesite and more calc-alkaline magma similar to mafic enclaves in the monzonite. Syn-intrusion structures in the monzonite indicate emplacement during E–W sinistral strike-slip faulting that created local transtensional deformation, providing accommodation for a Dikeos magma reservoir. A change in the style of deformation in the Late Miocene led to NW-striking extension in the footwall, occupied by mafic dykes and mineralized veins, and extensional detachment of the hanging wall, resulting in unroofing of the monzonite.

Modelo cinemático del control estructural 4D y metalogénesis del distrito auroargentífero Vetas-California (Santander, Colombia)

). The gold and silver deposits of the Vetas-California mining district are hosted in Paleozoic gneisses and Mesozoic granites of the Santander massif in the Eastern Cordillera of Colombia, far from the northern Andean volcanic arc. In the California area, there is ambiguity in defining the epithermal-type metallogenic model with high to intermediate sulfidation due to the presence of Late Triassic-Early Jurassic and Miocene magmatism. The best-known hydrothermal episodes comprise two early porphyritic-style phases: a) characterized by a propylitic alteration with molybdenite of the ~10.1 Ma-old La Mascota breccia, related to granodiorite porphyries and smaller-volume rhyodacitic dykes; and b) attributed to a magmatic-hydrothermal system due to phyllic alteration associated with quartz-pyrite veins with hydrothermal sericite dated at ~3.4 Ma. These two hydrothermal episodes were followed by four epithermal phases related to the development of multiphase hydrothermal breccias and quartz-alunite alteration, defined by bornite, covellite, and chalcopyrite, alongside wolframite, enargite, and sphalerite, with gold and silver mineralization between ~2.6 and ~ 1.6 Ma. To understand the relationship of the emplacement of the porphyries and the subsequent auro-argentiferous enrichments to the deformation front observed in the Pamplona buttress, to the east of the Santander Massif, a 4D structural model of the mineralization is formulated here. This model is based on the spatial analysis of the stresses responsible for the La Baja-Angostura dextral fault in terms of the regional kinematics of the Bucaramanga sinistral fault. It is considered that the SW sector of the deposit area corresponds to a well-defined, tabular, sub-vertical body of the La Mascota breccia. On the other hand, the NE sector in the La Baja-Angostura fault is expressed as a topographically elevated right horsetail splay, which controls the more disseminated distribution of the mineralization by means of veinlet swarms arranged in an ~E-W direction. The disseminated mineralization of Vetas-California was probably favored by late thermal expansion-contraction events in the gneissic sockets, which allowed the accumulation and precipitation of boiling solutions rich in volatiles and elements of economic interest.

Upper Miocene volcanic ash layers from central Italy: tracking down the volcanic source

Upper Miocene volcanic ash layers from central Italy: tracking down the volcanic source

Mio-pliocene evolution of deep-water channels on the northeastern Bengal fan: Records of signals of Himalaya uplift and South Asian Monsoon from source areas

Submarine channels, acting as major conduits for delivering terrestrial sediments into the deep sea, are sensitive to source-region uplift and climate variations. Using new 3D seismic data, previously published paleoenvironmental data, and IODP/ODP data, we characterize stratigraphic time changes in deep-water channels on the northeastern Bengal Fan and suggest that their long-term evolution relates to contemporaneous changes of Himalaya uplift and South Asian Monsoon. Our results show that around the boundary between the Miocene and Pliocene Epochs, the sedimentary characteristics of deep-water channels were changing at about the same time as decreased rates of Himalaya uplift and changes in the monsoonal climate. It is strongly suggested that environmental signals could successfully propagate from the Himalaya to the deep sea and play a dominant control on the long-term evolution of deep-water channels. During the late Miocene, orogen-wide and substantial Himalaya uplift and humid monsoonal climates (dominance of summer monsoon) generated a sediment supply with high fluxes, which allowed coarser-grained sediments to reach further into the basin and thus caused the formation of larger-scale, non-leveed erosional channels that have avulsion and confluence phenomena. After the Miocene, local and limited Himalaya uplift and arid monsoonal climates (dominance of winter monsoon) led to a sediment supply with low fluxes, during which only finer-grained sediments reached deep water and gave rise to smaller-scale, leveed aggradational channels that mainly have splay deposits. Between the late-Miocene and post-Miocene periods, detrital zircon U-Pb ages of Bengal Fan samples from IODP cores reflect a sediment provenance change from the mixing of Brahmaputra and Ganges Rivers to the end-member of Brahmaputra River, broadly coeval with the variations of Himalaya uplift and South Asian Monsoon. Moreover, Bengal Fan cores record a Mio-Pliocene decrease in sedimentation rates and maximum grain sizes and an increase in mudstone contents, consistent with the variations of sediment yield in the source catchments. Observations and interpretations from the current study, therefore, contribute to a better understanding of how the long-term evolution of submarine channels respond to tectonic and climatic perturbations in source areas and may help recognizing similar process-response relationships in other areas.

Phylogeny, historical biogeography and climate niche differentiation in extant species of Ceratophryidae (Anura, Hyloidea) frogs in South America

Ceratophryidae is a family of frogs containing twelve extant species distributed in South America. Several studies have been carried out concerning the systematics, morphology, karyotypes, and behaviour within this monophyletic family. However, little is known about the historical biogeography and the divergence in time of this group. Here, we present an updated phylogeny, along with a calibrated chronogram, to analyse the historical biogeographical pattern and climate niche differentiation among extant species of Ceratophryidae (Anura: Hyloidea) frogs in South America. A phylogeny based on morphological and genetic data was obtained from 256 morphological characters, six mitochondrial and eight nuclear genes for up to a total of 8428 characters in the homological matrix. Our results indicate that the genus Ceratophrys is sister to the clade Chacophrys + Lepidobatrachus. The divergence of the crown group of Ceratophryidae is estimated to have occurred 19.2 Ma at the beginning of the Miocene, with recent cladogenetic events related to the late Miocene (10.18–13.70 Ma) and the Pliocene (~5.3–2.6 Ma). Moreover, the Cerrado region is estimated to be the ancestral area of the family, as well for the genera Chacophrys and Lepidobatrachus. In addition, temperature seasonality and annual precipitation play a major role in the niche differentiation of extant species within Ceratophryidae. In conclusion, our data suggest multiple dispersal and vicariance events originating from the Cerrado region in the early Miocene, and recognize the role of the environment in the differentiation of the ecological niches among extant species of this family in South America. Ceratophryidae is hypothesized to have originated in the semi-arid Cerrado region in the early Miocene (~19.2 million years ago), from where species have diversified by independent dispersal and vicariance events across other South American regions. Ceratophrys frogs are phylogenetically recovered as the sister taxon to the clade comprising Chacophrys and Lepidobatrachus. Three lineages of Lepidobatrachus and four lineages from Ceratophrys diverged in the Pliocene, around 5.3 to 2.6 million years ago. Niche differentiation among extant species of Ceratophryidae appears to reflect roles for temperature seasonality and annual precipitation.

Neogene–Quaternary paleoenvironments and kerogen assessment of the NDO B-1 well, offshore Nile Delta, Egypt, Eastern Mediterranean: palynological evidence

Palynofacies and palynological investigations conducted on the Neogene–Quaternary succession from the NDO B-1 well, located in the offshore Nile Delta, Egypt, in the Eastern Mediterranean, suggest generally shallow marine (neritic) conditions. These environments are manifested by the overall palynofacies composition and the occurrence of dinoflagellate cysts (e.g., Spiniferites spp., Lingulodinium spp., Hystrichokolpoma spp., Homotryblium spp. and Selenopemphix spp.). Neritic environments are suggested for the lower and middle Miocene Sidi Salim, and the Pliocene to Pleistocene upper Kafr El Sheikh, El Wastani and Mit Ghamr formations, while shallower, coastal to inner neritic settings were interpreted for the late Miocene (Qawasim and Rosetta formations) and early Pliocene (Abu Madi and lower Kafr El Sheikh formations). Anoxic conditions existed during the deposition of the studied well succession, which can be seen from the occurrence of imprints of pyrite crystals and some types of oxygen-sensitive dinoflagellate cysts. The palynofacies fluctuated repeatedly between Amorphous Organic Matter (AOM)-dominated and phytoclast-dominated intervals, of kerogen types II and III, respectively. The spore coloration index (SCI) of indigenous thin-walled palynomorphs confirms thermally mature sediments, generative of dry gas and wet gas/condensates. Reworking during the deposition of the upper Sidi Salim, Qawasim and Rosetta formations is inferred from the occurrence of Cretaceous dinoflagellate cysts and pollen.

Comparison of organelle genomes between endangered mangrove plant Dolichandrone spathacea to terrestrial relative provides insights into its origin and adaptative evolution.

Dolichandrone spathacea is a mangrove associate with high medicinal and ecological values. However, due to the dual-pressure of climate change and human activities, D. spathacea has become endangered in China. Moreover, misidentification between D. spathacea and its terrestrial relative D. cauda-felina poses further challenges to field protection and proper medicinal usage of D. spathacea. Thus, to address these problems, we sequenced and assembled mitochondrial (mt) and chloroplast (cp) genomes for both D. spathacea and D. cauda-felina. Comparative analysis revealed apparently different size and scaffold number between the two mt genomes, but a high similarity between the cp genomes. Eight regions with high sequence divergence were identified between the two cp genomes, which might be used for developing candidate DNA markers for distinguishing the two species. The splitting between D. spathacea and D. cauda-felina was inferred to occur at ~6.8 - 7.7 million years ago (Mya), which may be driven by the environment fluctuations in late Miocene. In the cp genome, 12 genes related to the expression of photosynthesis-associated proteins were detected with signatures of positive selection, which may contribute to the origin and evolutionary adaptation of Dolichandrone mangrove species. These new findings do not only enrich organelle genomic resources of Dolichandrone species, but also provide important genetic clues for improving the conservation and proper usage of endangered mangrove associate D. spathacea.