This paper describes a new quality inspection method of woven fabrics. This method is based on Laser induced fluorescence (LIF). LIF spectra were measured by a developed measuring system. Defect such as soil, and the degree of dye were the focus in this paper. Typical standard cloth was used as a sample and synthesized sweat and human lipid was used as model soil on the standard cloth. Hues of sample clothes dyed at various levels and the LIF intensities were examined by using the conventional colorimetric method values as parameters. Appropriate calibration models to obtain the concentration of the dirt elements or degree of dye were studied. This paper also describes the application of this method to estimate the real human dirt that includes sweat and lipid.