Larval Sea Lamprey Research Articles

Presence of glutamate, glycine, and γ‐aminobutyric acid in the retina of the larval sea lamprey: Comparative immunohistochemical study of classical neurotransmitters in larval and postmetamorphic retinas

The neurochemistry of the retina of the larval and postmetamorphic sea lamprey was studied via immunocytochemistry using antibodies directed against the major candidate neurotransmitters [glutamate, glycine, gamma-aminobutyric acid (GABA), aspartate, dopamine, serotonin] and the neurotransmitter-synthesizing enzyme tyrosine hydroxylase. Immunoreactivity to rod opsin and calretinin was also used to distinguish some retinal cells. Two retinal regions are present in larvae: the central retina, with opsin-immunoreactive photoreceptors, and the lateral retina, which lacks photoreceptors and is mainly neuroblastic. We observed calretinin-immunostained ganglion cells in both retinal regions; immunolabeled bipolar cells were detected in the central retina only. Glutamate immunoreactivity was present in photoreceptors, ganglion cells, and bipolar cells. Faint to moderate glycine immunostaining was observed in photoreceptors and some cells of the ganglion cell/inner plexiform layer. No GABA-immunolabeled perikarya were observed. GABA-immunoreactive centrifugal fibers were present in the central and lateral retina. These centrifugal fibers contacted glutamate-immunostained ganglion cells. No aspartate, serotonin, dopamine, or TH immunoreactivity was observed in larvae, whereas these molecules, as well as GABA, glycine, and glutamate, were detected in neurons of the retina of recently transformed lamprey. Immunoreactivity to GABA was observed in outer horizontal cells, some bipolar cells, and numerous amacrine cells, whereas immunoreactivity to glycine was found in amacrine cells and interplexiform cells. Dopamine and serotonin immunoreactivity was found in scattered amacrine cells. Amacrine and horizontal cells did not express classical neurotransmitters (with the possible exception of glycine) during larval life, so transmitter-expressing cells of the larval retina appear to participate only in the vertical processing pathway.

Comparison of Larval Sea Lamprey Life History Characteristics in a Lampricide‐Treated Tributary and Untreated Tributary System of Lake Champlain

AbstractControl of sea lampreys Petromyzon marinus in Lake Champlain and the Laurentian Great Lakes is dependent primarily on selective lampricides that reduce the density of sea lamprey larvae in tributary streams. After lampricide treatment, recolonized larval sea lampreys may experience enhanced growth from density‐dependent factors, which may lead to transformation at a larger size and younger age than would have occurred before treatment. In this study, the effects of lampricide treatments on larval sea lamprey life history characteristics were compared between Lewis Creek (Vermont), a treated stream, at 4–5 years posttreatment and the untreated Pike River–Morpion Stream system (Quebec, Canada). Sea lamprey habitat was measured in each stream at approximately 100 randomly selected transects, and larval density was determined using a backpack electrofishing unit during 1999 and 2000. Larval sea lamprey density was approximately threefold higher in Lewis Creek than in the Pike River and Morpion Stream in both years. Growth measured in length and weight indicated that neither treatment nor low density created conditions for faster growth. During 1999, transformers were, on average, shorter and younger in Lewis Creek (131 mm; 100% age 4) than in the Pike River–Morpion Stream (143 mm; 100% age 5). During 2000, the majority (75%) of transformers were age 5 in all streams. Weights of age‐4 transformers in Lewis Creek (1999 and 2000) and the Pike River (2000) were higher than those of age‐4 ammocoetes in those streams. Our results suggest that the first year‐class to become reestablished after lampricide application may transform at a younger age and smaller size than fish of the same age‐class in an untreated stream. Treatment intervals must adapt to changes in age at transformation to prevent transformers from leaving Lake Champlain and Great Lakes tributaries.

Dispersal of sea lamprey larvae during early life: relevance for recruitment dynamics

Dispersal is an important early life history process that influences fish population dynamics and recruitment. We studied larval sea lamprey (Petromyzon marinus) dispersal by combining spatially explicit field sampling, genetic methods, and laboratory experiments to investigate how far sea lamprey larvae can disperse away from nests during their first growing season; subsequent dispersal by age 1 of sea lamprey; and the effect of density on larval dispersal. In two study streams sea lamprey larvae were observed to have moved >150 m downstream from the most likely source nest within 2–3 weeks of hatching. Conversely, randomization trials suggested that for both streams age 0 larvae were found closer to full siblings than would be expected if dispersal was not constrained by distance. Restricted dispersal was also observed for age 1 larvae in five streams, although for this age class full siblings were more commonly found to be separated by >1,000 m. Laboratory experiments indicated a significant effect of density on the movement of larval sea lamprey, with more larval movement at higher densities. Temperature also affected movement significantly, with reduced larval movements at cooler temperatures. Our findings suggest that larval sea lamprey dispersal is sufficient to minimize the likelihood of strong density-dependent effects on recruitment, even with large population sizes.

Calbindin and calretinin immunoreactivity in the retina of adult and larval sea lamprey

The presence of calretinin and calbindin immunoreactivity is studied in the retina of larval and adult lamprey and their respective distributions are compared. Calretinin distribution is also studied in the retina of transforming stages. Western blot analysis in brain extracts showed a 29-kDa band with both polyclonal anti-calbindin and anti-calretinin antibodies. Calbindin and calretinin immunoreactivity has shown a partially different distribution. In the adult retina large and small bipolar cells, with respectively stratified or diffuse axons, the inner row of horizontal cells and ganglion cells and/or some amacrine cells were labeled with anti-calretinin antibody. The anti-calbindin antibody labels the same cell types except most of ganglion cells, but the label was less conspicuous. Therefore, the possible existence of these two calcium-binding proteins in the central nervous system of the sea lamprey could be discussed. In the differentiated central retina of larval lampreys, numerous calretinin immunoreactive bipolar and ganglion cells were observed, while, in the lateral retina, only ganglion cells were labeled, accordingly with the lack of differentiation of other neural cell types. CR-ir bipolar cells appeared in the retina by the stage 5 of transformation, i.e. about the time when differentiation of photoreceptors occurs. The comparison of the distribution of calretinin and calbindin between adult and larval central retina of lampreys shows striking differences that could be related to the different functionality of eyes in these two stages of the life cycle of lampreys. In addition, this is the first report on the presence of calcium-binding proteins in the larval and transforming lamprey retina, on the presence of calretinin- and calbindin-immunoreactive horizontal cells in adult lamprey retinas and on the differential stratification of bipolar cell terminals.

Chemoarchitecture of the dorsal column nucleus of the larval sea lamprey

We studied the organization of the dorsal column nucleus (DCN) of larval sea lamprey with immunohistochemical and tract-tracing techniques. Texas red-coupled dextran amine was injected into the spinal cord, which allowed tracing the dorsal column fibers and characterizing the DCN. The dorsal column fibers formed a dense tract coursing adjacent to the dorsal midline of the spinal cord to the caudal rhombencephalon alar plate. In larvae, most spinal cord dorsal cells and spinal ganglion perikarya, and many dorsal column fibers, were calretinin-immunoreactive. We delineated the DCN in the dorsomedial portion of the obex and preobecular alar plate. It consists of a periventricular neuronal cell layer and neurons scattered in the lateral neuropil and receives dorsal column fibers. After immunohistochemistry with antibodies against glutamate, glycine, and GABA numerous immunoreactive perikarya were observed in the DCN. In addition to glutamate-, glycine-, and GABA-immunoreactive processes, serotonin- and dopamine-immunoreactive fibers coursed in the neuropil of this nucleus. A few small calretinin-immunoreactive perikarya were also observed in the DCN. Our results reveal the presence of inhibitory and excitatory transmitters in neurons of the DCN, and suggest that dopamine and serotonin modulate the activity of this nucleus.

Influence of Larval Sea Lamprey Density on Transformer Life History Characteristics in Lewis Creek, Vermont

AbstractDensity‐dependent larval life history characteristics of sea lampreys Petromyzon marinus may have important management implications for sea lamprey control if life history attributes directly respond to conditions created by control strategies. Our objective was to examine the effects of larval sea lamprey density on sex ratio, size at metamorphosis, and age at metamorphosis in Lewis Creek, Vermont (one reach with naturally high larval density and one low‐density reach), and to determine the effects of lampricide treatments on these characteristics. Lewis Creek has a natural falls that divides the stream into two distinct larval sea lamprey populations; this barrier created areas of high and low densities in the stream before treatment. Lewis Creek was treated with lampricide in 1990 and 1994, and all visible dead sea lampreys were collected, identified, and counted. Sea lamprey transformers (metamorphosing larvae) were measured for length and weight and sex was determined. Larval habitat was quantified above and below the falls in 1999 and 2000, and larval density was quantified by electroshocking below the falls. During the first treatment, larval density above the falls was 64 times greater than density below the falls. Transformers above the falls were similar in weight, were 11% longer, and had a significantly higher proportion of females (75% versus 53%) than those collected below the falls. Density of sea lampreys below the falls increased after treatment. Transformers decreased in average length and weight between 1990 and 1994 and increased in length and weight between 1999 and 2000. The percentage of females fluctuated from 65% after the first treatment (1994) to 44% after the second treatment (1999) to 52% a year later (2000). Overall, higher larval density (below versus above the falls, after versus before treatment) was associated with smaller transformers and lower proportions of females.

Optical quality of the ocular lens of the sea lamprey (Petromyzon marinus) during the mature and transformer periods of life

While larval sea lampreys exist as eyeless filter feeders for several years, they transform into free-swimming juveniles (transformers) that attach parasitically to prey fish as they develop sexual maturity. This study examines lamprey lens development and optics and, since the lens is often the only refractive component of an aquatic eye, the data also provide an indication of visual ability during transformer and adult periods of life. Seven adult sea lampreys (0.40-0.55 m) and eight transformers (0.15-0.18 m) were sacrificed, the eyes removed and lenses dissected, measured, and placed in an automated laser scanning instrument. Back vertex focal length (spherical aberration) was measured for 14 beam positions across each lens by using a digital camera to record the position of the refracted beam. Transformer lenses exhibit positive spherical aberration, with average focal lengths varying from about 2.40 mm near the lens center and 1.06 mm at the lens periphery. On the other hand, the lenses from adults are largely corrected for spherical aberration, with average focal lengths varying from 2.19 mm to 2.44 mm. This result indicates that the younger lenses do not have a gradient refractive index necessary to mitigate the aberration and that further study of this model may reveal the relation between lens embryology and the development of such a gradient.

In situ Characterization of Gonadotropin- Releasing Hormone-I, -III, and Glutamic Acid Decarboxylase Expression in the Brain of the Sea Lamprey, Petromyzon marinus

The distribution of lamprey gonadotropin-releasing hormone (GnRH)-I and -III has been extensively characterized by immunocytochemistry in the forebrain of the sea lamprey, Petromyzon marinus. However, the cellular location of lamprey GnRH-III mRNA expression by in situ hybridization in the lamprey brain has not been determined. We show for the first time the location of expression of lamprey GnRH-III, as well as provide a more comprehensive in situ study of lamprey GnRH-I and glutamic acid decarboxylase (GAD; GABA-synthesizing enzyme) mRNA expression in the brain of the lamprey in different reproductive life stages. Colorimetric and dual-label fluorescent amplification methods of in situ hybridization were used on brain tissue sections of adult, juvenile, and larval sea lamprey. In each life stage of the lamprey, expression of lamprey GnRH-I was shown in the preoptic area (POA) and the hypothalamus forming the characteristic arc-like cell population extending from the preoptic nucleus (NPO) to the neurohypophysis. Lamprey GnRH-III expression was also seen in the POA of each life stage in close proximity to lamprey GnRH-I mRNA containing neurons. GAD expression was shown in distinct cell clusters in and around the POA, in the olfactory bulb, in the dorsal thalamus beneath the habenular region, and also in the ventral-medial hypothalamus stretching from the periventricular region to the anterior portion of the rhombencephalon. Using dual-label in situ hybridization, we have shown that lamprey GnRH-I and -III mRNA are colocalized in the same cells in the POA in adult lampreys. Dual-label in situ hybridization also showed close proximity of GAD mRNA containing neurons and GnRH containing neurons in the POA. These data suggest that γ-aminobutyric acid (GABA) may directly affect GnRH release in the brain of the sea lamprey.

Evidence That Petromyzontid Lampreys Employ a Common Migratory Pheromone That Is Partially Comprised of Bile Acids

This study examined whether the larval pheromone employed by adult sea lamprey (Petromyzon marinus) to locate spawning streams and known to be at least partially comprised of bile acids is also employed by other lamprey species. Both production and release of lamprey-specific bile acids, and sensitivity to them were examined in a wide variety of species. High pressure liquid chromatography and electrospray ionization/mass spectrometry (ESI-MS) found gallbladders from 10 species of European and North American lamprey to contain large quantities of petromyzonol sulfate (PS) together with much smaller quantities of allocholic acid (ACA) and petromyzonol (P). Evaluation of holding waters from three of these species using ESI-MS found all to contain large quantities of PS and lesser quantities of ACA in similar ratios. Electro-olfactogram recording from the olfactory systems of three parasitic lamprey species found all to detect PS and ACA with high sensitivity. Behavioral studies using migratory adult sea lamprey found them to be attracted to the odors of heterospecific larvae as well as conspecific larvae, both of which contained similar amounts of PS and ACA. Finally, adult silver lampreys (Ichthyomyzon unicuspis) were also found to be attracted to the odor of larval sea lamprey. Together, these results demonstrate that PS and ACA are commonly produced and released by larval petromyzontid lampreys and likely used as part of a common evolutionarily conserved pheromone. This scenario is reasonable because lampreys share similar larval and spawning habitat requirements, and their larvae derive no apparent benefit from producing compounds that serve as an attractant for adults.

Effect of Density and Age on Larval Sea Lamprey Growth and Survival in Three Lake Champlain Streams

ABSTRACT Age-1 and age-3 larval sea lamprey were separately held in circular cages (0.16 m2) with 30 cm of optimal habitat for one year. The five treatments for each age class were: 25, 50, 100, 150, and 200 larvae/m2. Growth of age-1 larvae was significantly higher than age-3 larvae (P<0.006), although survival was not significantly different between ages (P<0.546). Density treatments did not significantly affect growth and survival of the age treatments (P<0.195; P<0.868). These results suggest that age and biomass may be important variables to measure when examining potential demographic effects on larval lamprey life history characteristics.

The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys

A rapid, accurate and non‐invasive method to determine the sex of larval sea lampreys Petromyzon marinus, using wide‐field pulse‐scanning acoustic microscopy, is described. Cross‐sectional pulse‐echo scans were made with a high‐resolution acoustic microscope in 48 larvae (110–130 mm total length, LT), and the acoustic images generated showed such internal body structures as the gonad, intestine, kidneys, cardinal veins, notochord and musculature. Females were identified by the presence of a relatively large (1–1·5 mm diameter) ovary, which was considerably less reflective to the 15 to 25 MHz acoustic signals than the surrounding kidney tissue. Males were recognized by the lack of the large non‐reflective ovary and, in some cases, the appearance of a small (0·2–0·3 mm) testis with slightly stronger reflective properties than the kidney. Identification of sex was confirmed by optical microscopy following dissection, and in a blind test on an additional 10 specimens (121–168 mm LT), the acoustic method reliably identified sex in 100% of the larvae. These results indicate that acoustic microscopy can determine the sex of live sea lamprey larvae in c. 30 s per animal, a process which until now required dissection or invasive surgery.

HPLC and ELISA analyses of larval bile acids from Pacific and western brook lampreys

Comparative studies were performed on two native lamprey species, Pacific lamprey ( Lampetra tridentata) and western brook lamprey ( Lampetra richardsoni) from the Pacific coast along with sea lamprey ( Petromyzon marinus) from the Great Lakes, to investigate their bile acid production and release. HPLC and ELISA analyses of the gall bladders and liver extract revealed that the major bile acid compound from Pacific and western brook larval lampreys was petromyzonol sulfate (PZS), previously identified as a migratory pheromone in larval sea lamprey. An ELISA for PZS has been developed in a working range of 20 pg–10 ng per well. The tissue concentrations of PZS in gall bladder were 127.40, 145.86, and 276.96 μg/g body mass in sea lamprey, Pacific lamprey, and western brook lamprey, respectively. Releasing rates for PZS in the three species were measured using ELISA to find that western brook and sea lamprey released PZS 20 times higher than Pacific lamprey did. Further studies are required to determine whether PZS is a chemical cue in Pacific and western brook lampreys.

Density‐dependent growth of Sea Lamprey larvae: evidence for chemical interference

Summary Density can influence growth through exploitation competition and behavioural or chemical interference. Negative effects of population density on the growth rate of Sea Lamprey larvae have been reported in several field and laboratory studies, but the mechanisms involved are poorly understood. In this study the role of population density and waterborne‐mediated interference on the growth rate of Sea Lamprey larvae was assessed in two laboratory experiments. The effects of these factors were evaluated by comparing growth of larvae reared at three different densities (27, 75 and 128 individuals m−2) or in preconditioned water (i.e. containing Sea Lamprey larvae at the same three densities). Larval density had a negative effect on growth rates. Water conditioning had a negative but weaker effect on growth. Larvae reared in water preconditioned at the two higher densities showed a lower mass increase than those growing in water preconditioned at the lowest density. The results are consistent with the existence of some chemical or biological agent released into the surrounding water and influencing growth in larval Sea Lampreys. However, chemical interference is not the only factor influencing density‐dependent growth.

Classification of Lentic Habitat for Sea Lamprey ( Petromyzon marinus) Larvae Using a Remote Seabed Classification Device

Lentic populations of larval sea lampreys ( Petromyzon marinus) are suspected of being a major source of recruitment to parasitic stocks in some areas of the Great Lakes, and methods are needed to estimate habitat and population sizes. A deepwater electroshocker has been used to quantitatively assess larval sea lamprey populations in deepwater areas, however a method has not been developed to efficiently identify the most promising locations to sample in this environment. A remote seabed classification device (RoxAnn™) was used to identify soft substrates in a lentic area where sea lamprey larvae have been found in Batchawana Bay (Ontario) in eastern Lake Superior, and related those substrate types to larval distribution and occurrence. Presence of larvae was significantly related to substrate type, distance from the stream mouth, and slope of the lake bottom. Remote seabed classification would be a useful tool in the Sea Lamprey Control Program to identify the most promising locations to conduct larval surveys in lentic areas.

Acute Toxicity of TFM and a TFM/Niclosamide Mixture to Selected Species of Fish, Including Lake Sturgeon ( Acipenser fulvescens) and Mudpuppies ( Necturus maculosus), in Laboratory and Field Exposures

The toxicity of the lampricides 3-trifluoromethyl-4-nitrophenol (TFM) and 2′,5-dichloro-4′-nitrosalicylanilide (niclosamide) to non-target fishes has been a major point of concern since their use to control larval sea lamprey ( Petromyzon marinus) populations began in the early 1960s. The toxicity of TFM to several non-target fish species has been demonstrated in previous studies. However, little information is available on the toxicity of the TFM/1% niclosamide mixture. One species of particular concern is the lake sturgeon ( Acipenser fulvescens). Juvenile lake sturgeon of several size ranges were exposed to determine potential effects of the lampricides to individuals present in treatment streams. Sac fry were most resistant to the lampricides followed by fingerlings in the 200 to 225 mm size range. Swim-up fry and fingerlings less than 100 mm were the most sensitive. Concentrations that produced 50% mortality (LC50 s) in juvenile lake sturgeon of these smaller size ranges were at or near the minimum lethal concentrations (MLCs) required for effective control of larval sea lampreys. The mudpuppy ( Necturus maculosus), an amphibian native to several tributaries of the Great Lakes, have also become a species of interest in recent years. Laboratory tests conducted with TFM and a TFM/1% niclosamide mixture on adult mudpuppies indicate that although the amphibian is sensitive to the lampricides, an adequate margin of safety exists for adult mudpuppies to survive when exposed during stream treatments. Fifteen other fish species native to streams treated with lampricides were investigated in the laboratory to determine their sensitivity to the lampricides. Centrarchids, bluegill ( Lepomis macrochirus) and green sunfish ( Lepomis cyanellus) were the least sensitive to TFM, while ictalurids, black bullhead ( Ictalurus melas), channel catfish ( Ictalurus punctatus), and tadpole madtom ( Notorus gyrinus) were the most sensitive. On-site bioassays conducted before lampricide treatments also revealed that lake sturgeon, channel catfish, and whitefish ( Coregonus clupeaformis) were sensitive to the lampricides although considerably less sensitive compared to sea lamprey.

Selecting Great Lakes Streams for Lampricide Treatment Based On Larval Sea Lamprey Surveys

The Empiric Stream Treatment Ranking (ESTR) system is a data-driven, model-based, decision tool for selecting Great Lakes streams for treatment with lampricide, based on estimates from larval sea lamprey ( Petromyzon marinus) surveys conducted throughout the basin. The 2000 ESTR system was described and applied to larval assessment surveys conducted from 1996 to 1999. A comparative analysis of stream survey and selection data was conducted and improvements to the stream selection process were recommended. Streams were selected for treatment based on treatment cost, predicted treatment effectiveness, and the projected number of juvenile sea lampreys produced. On average, lampricide treatments were applied annually to 49 streams with 1,075 ha of larval habitat, killing 15 million larval and 514,000 juvenile sea lampreys at a total cost of $5.3 million, and marginal and mean costs of $85 and $10 per juvenile killed. The numbers of juvenile sea lampreys killed for given treatment costs showed a pattern of diminishing returns with increasing investment. Of the streams selected for treatment, those with > 14 ha of larval habitat targeted 73% of the juvenile sea lampreys for 60% of the treatment cost. Suggested improvements to the ESTR system were to improve accuracy and precision of model estimates, account for uncertainty in estimates, include all potentially productive streams in the process (not just those surveyed in the current year), consider the value of all larvae killed during treatment (not just those predicted to metamorphose the following year), use lake-specific estimates of damage, and establish formal suppression targets.

Estimating Lake-wide Abundance of Spawning-phase Sea Lampreys ( Petromyzon marinus) in the Great Lakes: Extrapolating from Sampled Streams Using Regression Models

Lake-wide abundance of spawning-phase sea lampreys ( Petromyzon marinus) can be used as one means to evaluate sea lamprey control efforts in the Great Lakes. Lake-wide abundance in each Great Lake was the sum of estimates for all streams thought to contribute substantial numbers of sea lampreys. A subset of these streams was sampled with traps and mark-recapture studies were conducted. When sea lampreys were captured in traps, but no mark-recapture study was conducted, abundance was estimated from a relation between trap catch and mark-recapture estimates observed in other years. In non-sampled streams, a regression model that used stream drainage area, geographic region, larval sea lamprey, production potential, the number of years since the last lampricide treatment, and spawning year was used to predict abundance of spawning-phase sea lampreys. The combination of estimates from sampled and non-sampled streams provided a 20-year time series of spawning-phase sea lamprey abundance estimates in the Great Lakes.

Response of Larval Sea Lampreys ( Petromyzon marinus) to Pulsed DC Electrical Stimuli in Laboratory Experiments

Four electrical factors that are used in pulsed DC electrofishing for larval sea lampreys ( Petromyzon marinus) were evaluated in two laboratory studies to determine the optimal values to induce larval emergence over a range of water temperatures and conductivities. Burrowed larvae were exposed to combinations of pulsed DC electrical factors including five pulse frequencies, three pulse patterns, and two levels of duty cycle over a range of seven voltage gradients in two separate studies conducted at water temperatures of 10, 15, and 20°C and water conductivities of 25, 200, and 900 μS/cm. A four-way analysis of variance was used to determine significant (α = 0.05) influences of each electrical factor on larval emergence. Multiple comparison tests with Bonferroni adjustments were used to determine which values of each factor resulted in significantly higher emergence at each temperature and conductivity. Voltage gradient and pulse frequency significantly affected emergence according to the ANOVA model at each temperature and conductivity tested. Duty cycle and pulse pattern generally did not significantly influence the model. Findings suggest that a setting of 2.0 V/cm, 3 pulses/sec, 10% duty, and 2:2 pulse pattern seems the most promising in waters of medium conductivity and across a variety of temperatures. This information provides a basis for understanding larval response to pulsed DC electrofishing gear factors and identifies electrofisher settings that show promise to increase the efficiency of the gear during assessments for burrowed sea lamprey larvae.

Agreement Among Observers Classifying Larval Sea Lamprey ( Petromyzon marinus) Habitat

Estimates of larval sea lamprey ( Petromyzon marinus) abundance are used to rank Great Lakes tributaries for lampricide treatment. Observers subjectively stratify habitat into three categories: type I = preferred, type II = acceptable, type III = unacceptable. Agreement was evaluated among eight observers classifying habitats in small discrete plots in two Lake Superior tributaries, the Rock and Chocolay rivers, and among four observers classifying and measuring the amount of each habitat type along random transects in the Rock River. Agreement among the eight observers classifying habitat plots was high (Chocolay, κ = 0.742 and Rock, κ = 0.785). The amounts of types I, II, and III habitat estimated were statistically different among observers. However, the amount of variability found in the classification and measurement of habitat by observers had little effect on the ranking of 51 streams considered for lampricide treatment.

Relative Contributions of Sampling Effort, Measuring, and Weighing to Precision of Larval Sea Lamprey Biomass Estimates

We developed two weight-length models from 231 populations of larval sea lampreys (Petromyzon marinus) collected from tributaries of the Great Lakes: Lake Ontario (21), Lake Erie (6), Lake Huron (67), Lake Michigan (76), and Lake Superior (61). Both models were mixed models, which used population as a random effect and additional environmental factors as fixed effects. We resampled weights and lengths 1,000 times from data collected in each of 14 other populations not used to develop the models, obtaining a weight and length distribution from reach resampling. To test model performance, we applied the two weight-length models to the resampled length distributions and calculated the predicted mean weights. We also calculated the observed mean weight for each resampling and for each of the original 14 data sets. When the average of predicted means was compared to means from the original data in each stream, inclusion of environmental factors did not consistently improve the performance of the weight-length model. We estimated the variance associated with measures of abundance and mean weight for each of the 14 selected populations and determined that a conservative estimate of the proportional contribution to variance associated with estimating abundance accounted for 32% to 95% of the variance (mean = 66%). Variability in the biomass estimate appears more affected by variability in estimating abundance than in converting length to weight. Hence, efforts to improve the precision of biomass estimates would be aided most by reducing the variability associated with estimating abundance.