Filariasis transmitted by vector mosquito Culex sp is one health problem in Bandung. Prevention efforts need to be done either by controlling mosquito larvae Culex sp using nabati larvicides such as karika seed extract (Carica pubescens). Karika chemical content in seeds consisting of saponins, alkaloids, and terpenoids which cytotoxic effect against mosquito larvae Culex sp. This study aimed to determine the effect of various concentrations of karika seed extract (Carica pubescens) on the death of the mosquito larvae Culexsp.The research is a field-scale experiments. The population in this study are all the larvae of Culex sp were bred at the Laboratory School of Biological Sciences and Technology ITB and the sample in this study the majority of mosquito larvae Culex sp with a sample size of 475 individuals. The sampling technique in this research is purposive sampling. This research data collection techniques are observation and measurement. Data collected include the number of larva Culex sp mortality,the results of measurements of air temperature, air humidity, water temperature and pH of the water. The data obtained and analyzed using univariate and bivariate using test, Kruskal-Wallis t test andtest. Mann Whitney Results showed the number of larvae of death Culex sp at a concentration of 0.2% on average 14 individuals (54%), at a concentration of 0.3%, an average of 19 individuals (76%) and at a concentration of 0.4% on average 25 tail (98%). The results of the bivariate analysis (Kruskal-Wallis) obtained result p <α (0.001 <0.05), so there is the effect of varying concentrations of affixing karika seed extract (Carica pubescens)on the death of larvae of Culexsp.This research could be useful in the implementation of vector control programs and vermin, especially larvae of Culex sp and suggested using environmentally friendly natural larvicidal from karika seed extract (Carica pubescens).