Abstract While some species thrive in urban areas, many are absent from such environments. Those that are successful often have high behavioural flexibility that allows them to exploit new niches in a human-modified landscape. Northern myotis (Myotis septentrionalis) is an endangered bat species rarely identified in urban areas, though it is unclear whether this is due to absence or difficulties in surveying. We investigated the ecology of a population of northern myotis within Canada’s largest conurbation, including reproductive status, roosting preference, and movements. Using capture surveys, we confirmed the presence of reproductive females and healthy juveniles over two seasons. Using radio telemetry and acoustic surveys, we identified a cluster of tree roosts in the centre of the forest, and foraging areas concentrated around waterways within the bounds of the forest. These observations suggest the roosting and movement ecology of this population is similar to that observed for this species in rural environments, despite the urban surroundings. Our results suggest that northern myotis is not a synurbic species but can occur within urbanized environments when suitable habitat is available. We suggest that large forest patches with mature, interior forest cover are likely to be an important resource for northern myotis, and they will be vulnerable to the loss or fragmentation of these features in rapidly urbanizing landscapes. These findings are highly relevant to the ecology and preservation of northern myotis and present a case for greater consideration of this species in urban forests.