Large Field Study Research Articles

Driving Consumer Engagement for Circular Luxury Products: Two Large Field Studies on the Role of Regulatory Mode Language

ABSTRACTThis research investigates the effect of language on the marketing of circular luxury products. Circular economy principles are becoming important for luxury brands as consumers demand more circular and sustainable alternatives to traditional products. However, luxury literature is divergent on whether communicating circular economy principles with luxury products leads to positive or negative consumer outcomes. This is due to the perceived incompatibility of sustainability and luxury. This research investigates the influence of language used with circular luxury products in sales descriptions of second‐hand circular luxury products (Study 1) and language effects of sustainability communication on social media (Study 2) to establish impact on consumer engagement. Our analysis of combined N = 921,939 observations demonstrates that product descriptions and social media posts communicating circular principles lead to decreased consumer engagement. This is a major problem for the marketing of circular luxury products. However, this effect can be overcome by using predominant locomotion, progress‐focused language that creates a regulatory fit with circular luxury products, increasing consumer engagement. Theoretically this study shows the importance of predominant locomotion language in the marketing of circular luxury products. Luxury marketers can utilise predominant locomotion language in their brand communication to increase consumer engagement with these products.

Large‐scale forest disturbance and associated management shape bird communities in Central European spruce forests

Abstract In Central Europe, natural forest disturbance is increasing due to climate change and the legacies of past forest management. The resulting early successional forest stages shape habitat conditions and availability for forest‐dwelling species. How disturbance, pre‐disturbance forest management such as beech underplanting in conifer stands and post‐disturbance management, such as salvage logging, shape biodiversity is poorly understood. In a large field study, we quantified how the large‐scale loss of mature spruce plantations after natural disturbance affects bird diversity and abundance in Central Europe and how this is moderated by pre‐ and post‐ disturbance management. In a Bayesian community distance sampling approach, we conducted field surveys on 182 plots in a stratified design, comparing bark beetle and windthrow disturbances as well as two management treatments, (i) underplanting with beech before the disturbance occurred versus no underplanting and (ii) salvage logging versus no post‐disturbance logging. We used undisturbed mature spruce plantations as a control. Bird diversity was higher in disturbed forests than in undisturbed spruce plantations and was affected little by management. Bayesian community distance sampling models revealed that responses were highly species‐specific: shrub‐associated birds profited from disturbance in general and reached highest densities where beech had been planted under the spruce canopy before the disturbance occurred, whereas open‐habitat and farmland birds reached higher densities on salvage‐logged sites. Only few typical species of coniferous forests persisted at disturbed sites. Synthesis and applications. With increasing forest disturbance, pre‐ and post‐disturbance management can promote target bird species. Shrub‐associated species can be promoted by underplanting, open habitat‐adapted species by salvage logging and species depending on vertical structures by no intervention. For maximising biodiversity benefits at a landscape scale, underrepresented management practices (no intervention) should be promoted to ensure a balanced forest management and to maintain diverse post‐disturbance bird communities.

Business-to-Investor Marketing: The Interplay of Costly and Costless Signals

Marketing to investors—especially when seeking funding for startups—is unique, with investors facing extreme uncertainty. This study uses foundational work in marketing, economics, management, finance, and psychology, as well as theories-in-use development with angel and venture capital investors, to build a business-to-investor marketing theory. The theory proposes that investors rely on marketing signals from startups, whether they are costly (financial, social, human, and intellectual resource endowments) or costless (verbal passion and concreteness). Results of a large quantitative field study of 5,334 written proposals from startups show that costly and costless signals have interactive effects on investor acceptance. The natural entrepreneurial tendency to compensate for a lack of costly signals with the use of passionate language backfires, reducing investor acceptance. Only when costly signals are communicated does a greater use of passion increase investor acceptance. Further, written proposals should be moderately concrete when they lack costly signals and should be formulated abstractly when plenty of costly signals can be offered. These contingencies provide insights into costly–costless signal interdependence in business-to-investor marketing and suggest how startups can optimize their written proposals for investor acceptance.

The Use of Machine Learning to Predict Prevalence of Subclinical Mastitis in Dairy Sheep Farms.

The objective of the study was to develop a computational model with which predictions regarding the level of prevalence of mastitis in dairy sheep farms could be performed. Data for the construction of the model were obtained from a large Greece-wide field study with 111 farms. Unsupervised learning methodology was applied for clustering data into two clusters based on 18 variables (17 independent variables related to health management practices applied in farms, climatological data at the locations of the farms, and the level of prevalence of subclinical mastitis as the target value). The K-means tool showed the highest significance for the classification of farms into two clusters for the construction of the computational model: median (interquartile range) prevalence of subclinical mastitis among farms was 20.0% (interquartile range: 15.8%) and 30.0% (16.0%) (p = 0.002). Supervised learning tools were subsequently used to predict the level of prevalence of the infection: decision trees, k-NN, neural networks, and Support vector machines. For each of these, combinations of hyperparameters were employed; 83 models were produced, and 4150 assessments were made in total. A computational model obtained by means of Support vector machines (kernel: 'linear', regularization parameter C = 3) was selected. Thereafter, the model was assessed through the results of the prevalence of subclinical mastitis in 373 records from sheep flocks unrelated to the ones employed for the selection of the model; the model was used for evaluation of the correct classification of the data in each of 373 sets, each of which included a test (prediction) subset with one record that referred to the farm under assessment. The median prevalence of the infection in farms classified by the model in each of the two categories was 10.4% (5.5%) and 36.3% (9.7%) (p < 0.0001). The overall accuracy of the model for the results presented by the K-means tool was 94.1%; for the estimation of the level of prevalence (<25.0%/≥25.0%) in the farms, it was 96.3%. The findings of this study indicate that machine learning algorithms can be usefully employed in predicting the level of subclinical mastitis in dairy sheep farms. This can facilitate setting up appropriate health management measures for interventions in the farms.

Clinical Characteristics and Outcomes of Limb Fractures in Saudi Children.

Children's bones are at high risk of fracture as they grow. The clinical characteristics of fractures in children differ from those in adults. Studying fractures in healthy children is critical for identifying cases of fragility fractures. The aim of this study was to assess the clinical characteristics of limb fractures as well as clinical indicators of fracture healing outcomes in healthy Saudi children seen in an emergency room. A retrospective review of the treatment course of all pediatric fractures and related factors treated at King Abdullah Specialist Children's Hospital (KASCH) in Riyadh between 2016 and 2018 was conducted. Children with a primary bone disorder or chronic comorbidities known to affect bone health were excluded. The study included 143patients(mean age ±SD =8.23 + 3.76 years), and 71% (n= 102) were males. Motor vehicle accidents (MVAs) were the most common mechanism of injury, accounting for 50 (35%) cases, followed by fall injuries, sports injuries, and pedestrian accidents at 45 (31.4%), 16 (11.2%), and 13 (9.1%), respectively. A total of 178 fractures were reported, with the femur (n= 75, 42.1%) being the most common of the reported fracture sites, followed by the forearm (n= 44, 24.7%). The most common type of fracture was transverse fracture (n= 96, 54% of patients). Vitamin D levels were measured in 53/143 cases. Of these, vitamin D deficiency was found in 38 (71.7%) patients. The average time for fracture healing was 32.9 ± 30.2 weeks. The mechanisms of injury, including MVAs and sports injuries, as well as femur and forearm fractures, were clinical factors that were independently associated with a longer duration of fracture healing time (p< 0.001), but age, gender, and vitamin D status were not associated with that outcome. MVAs andfall injurieswere the most common causes of fracture in our patients. MVAs and sports injurieswere associated with prolonged healing time. Large prospective, multicenter, or field studies may be required to further explore clinical characteristics, outcomes, and environmental factors.

Metal and oxidative potential exposure through particle inhalation and oxidative stress biomarkers: a 2-week pilot prospective study among Parisian subway workers

ObjectiveIn this pilot study on subway workers, we explored the relationships between particle exposure and oxidative stress biomarkers in exhaled breath condensate (EBC) and urine to identify the most relevant biomarkers for a large-scale study in this field.MethodsWe constructed a comprehensive occupational exposure assessment among subway workers in three distinct jobs over 10 working days, measuring daily concentrations of particulate matter (PM), their metal content and oxidative potential (OP). Individual pre- and post-shift EBC and urine samples were collected daily. Three oxidative stress biomarkers were measured in these matrices: malondialdehyde (MDA), 8-hydroxy-2′deoxyguanosine (8-OHdG) and 8-isoprostane. The association between each effect biomarker and exposure variables was estimated by multivariable multilevel mixed-effect models with and without lag times.ResultsThe OP was positively associated with Fe and Mn, but not associated with any effect biomarkers. Concentration changes of effect biomarkers in EBC and urine were associated with transition metals in PM (Cu and Zn) and furthermore with specific metals in EBC (Ba, Co, Cr and Mn) and in urine (Ba, Cu, Co, Mo, Ni, Ti and Zn). The direction of these associations was both metal- and time-dependent. Associations between Cu or Zn and MDAEBC generally reached statistical significance after a delayed time of 12 or 24 h after exposure. Changes in metal concentrations in EBC and urine were associated with MDA and 8-OHdG concentrations the same day.ConclusionAssociations between MDA in both EBC and urine gave opposite response for subway particles containing Zn versus Cu. This diverting Zn and Cu pattern was also observed for 8-OHdG and urinary concentrations of these two metals. Overall, MDA and 8-OHdG responses were sensitive for same-day metal exposures in both matrices. We recommend MDA and 8-OHdG in large field studies to account for oxidative stress originating from metals in inhaled particulate matter.

Am I next? Men and women's divergent justice perceptions following vicarious mistreatment.

Though we would like to believe that people universally consider workplace mistreatment to be an indicator of injustice, we describe why bystanders can react to justice events (in this study, vicariously observing or becoming aware of others being mistreated) with diverging perceptions of organizational injustice. We show that a bystander's gender and their gender similarity to the target of mistreatment can produce identity threat, which affects whether bystanders perceive the overall organization to be rife with gendered mistreatment and unfairness. Identity threat develops via two pathways-an emotion-focused reaction and a cognitive-focused processing of the event-and each pathway distally relates to different levels of bystanders' justice perceptions. We test these notions in three complementary studies: two laboratory experiments (N = 563; N = 920) and a large field study (N = 8,196 employees in 546 work units). Results generally show that bystanders who are women or similar in gender to the target of mistreatment reported different levels of emotional and cognitive identity threat that related to psychological gender mistreatment climate and workplace injustice following the incident as compared to men and those not similar in gender to the target. Overall, by integrating and extending bystander theory and dual-process models of injustice perceptions, through this work, we provide a potentially overlooked reason why negative behaviors like incivility, ostracism, and discrimination continue to occur in organizations. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Chemical Weathering Patterns of Diluted Bitumen Spilled into Freshwater Limnocorrals.

Due to the sudden nature of oil spills, few controlled studies have documented how oil weathers immediately following accidental release into a natural lake environment. Here, we evaluated the weathering patterns of Cold Lake Winter Blend, a diluted bitumen (dilbit) product, by performing a series of controlled spills into limnocorrals installed in a freshwater lake in Northern Ontario, Canada. Using a regression-based design, we added seven different dilbit volumes, ranging from 1.5 to 180 L, resulting in oil-to-water ratios between 1:71,000 (v/v) and 1:500 (v/v). We monitored changes in the composition of various petroleum hydrocarbons (PHCs), including n-alkanes, polycyclic aromatic hydrocarbons (PAHs), and oil biomarkers in dilbit over time, as it naturally weathered for 70 days. Depletion rate constants (kD) of n-alkanes and PAHs ranged from 0.0009 to 0.41 d-1 and 0.0008 to 0.38 d-1, respectively. There was no significant relationship between kD and spill volume, suggesting that spill size did not influence the depletion of petroleum hydrocarbons from the slick. Diagnostic ratios calculated from concentrations of n-alkanes, isoprenoids, and PAHs indicated that evaporation and photooxidation were major processes contributing to dilbit weathering, whereas dissolution and biodegradation were less important. These results demonstrate the usefulness of large scale field studies carried out under realistic environmental conditions to elucidate the role of different weathering processes following a dilbit spill.

Evaluation of a high‐speed planter in soybean production

AbstractTimely and quality planting of soybean is important to achieve maximum yield potential. Wet spring soil conditions and rain frequently shorten the time for farmers to plant crops within optimal soil conditions. New planter technology has been introduced that enables farmers to plant their fields faster and more precisely than with traditional planters. Large plot field studies were conducted in Indiana from 2015 to 2017 to evaluate a high‐speed planter at various planting speeds with multiple seeding rates on soybean. Seedling emergence, plant distribution, and final yield were evaluated. Three planting speeds [8, 12, and 16 kilometers per hour (kph)] and two seeding rates (222,000 and 321,000 seeds ha−1) were included in all years, and an additional planting speed and seeding rate were included in 2016 (20 kph and 420,000 seeds ha−1, respectively). Overall, planting speed did not impact soybean seedling emergence. Uniformity of plant spacing decreased slightly as the planting speed increased from 8 to 20 kph in 2016. Cool and wet conditions immediately after planting likely led to inconsistent emergence. Final grain yield was not affected by planting speeds or seeding rate except in 2017 when 12 kph planting speed yielded 0.25 Mg ha−1 higher than the other planting speeds. Increasing planting speed can be achieved without detrimentally affecting plant population, plant spacing, and yield in soybean.

A Preliminary Analysis of Thyrotropin Measurement from Finger Stick Dried Blood Spot with an Automated High-Throughput Immunoassay Analyzer.

Background: Hyper- and hypothyroidism are prevalent in Western countries and often go unnoticed for long periods. Thyrotropin (TSH) as a biomarker of thyroid dysfunction is regularly measured in venous plasma/serum. In newborn screening for congenital hypothyroidism, TSH is measured from dried blood spots (DBSs). DBS enables minimally invasive (at-home) sampling of a small blood volume that can be sent to diagnostic laboratories by regular mail. Methods: In this study, we included 109 patients who presented to the outpatient clinic of the University Medical Center Utrecht. Capillary finger stick was used to spot blood on a filter paper card and was dried. After extraction of TSH from DBS, method comparison with venous TSH was performed on an automated high-throughput immunoassay analyzer. Additional validation steps regarding stability, effect of hematocrit (Hct), precision, and limits of blank and quantitation were conducted according to corresponding Clinical and Laboratory Standards Institute evaluation protocol. Results: Method comparison of TSH from venous plasma versus finger stick DBSs showed an R2 [95% confidence interval] = 0.988 [0.986-0.990]. This enabled correct diagnosis of hypothyrotropinemia and hypothyroidism in 12 of 14 and 6 of 7 cases, respectively, with no false positives. Furthermore, TSH from DBS was stable for at least 4 days at temperatures between -20°C and +30°C, and the maximum decrease of eluate TSH was 1.13% for 1% increase in Hct. Conclusions: TSH from DBS may be accurately measured on an automated high-throughput immunoassay analyzer and could be used to diagnose hypothyroidism and, for the first time, hypothyrotropinemia. This method, when confirmed in larger field studies, may enable individuals to engage in (at-home) sampling of blood on DBSs for telediagnostics, screening programs, patient follow-up, and medication management.

Self-presentation in medicine: How language patterns reflect physician impression management goals and affect perceptions

Self-presentation in medicine: How language patterns reflect physician impression management goals and affect perceptions

Indoor Air Quality and Learning: Evidence from A Large Field Study in Primary Schools

Indoor Air Quality and Learning: Evidence from A Large Field Study in Primary Schools

Indoor Air Quality And Learning: Evidence from a Large Field Study in Primary Schools

Indoor Air Quality And Learning: Evidence from a Large Field Study in Primary Schools

Blood draw site and analytic device influence hemoglobin measurements.

Anemia is a continuing global public health concern and a priority for international action. The prevalence of anemia is estimated from the hemoglobin (Hb) levels within target populations, yet the procedures for measuring Hb are not standardized and different approaches may result in discrepancies. Several analytical variables have been proposed to influence Hb measurements, but it is difficult to understand the impact on specific variables from large population or field studies. Therefore, we designed a highly controlled protocol that minimized most technical parameters to specifically investigate the impact of blood draw site and analytic device on Hb measurements. A diverse cohort of sixty healthy adults each provided a sequential capillary and venous blood sample that were measured for Hb using an automated hematology analyzer (ADVIA-2120) and two point-of-care devices (HemoCue 201+ and HemoCue 301). Comparing blood draw sites, the mean Hb content was 0.32-0.47 g/dL (2-4%) higher in capillary compared to venous blood from the same donors. Comparing different Hb measuring instruments, the mean Hb content was 0.19-0.46 g/dL (1-4%) higher measured with HemoCue devices compared to ADVIA-2120 in both capillary and venous blood from the same donors. The maximum variance in measurement was also higher with HemoCue devices using blood from venous (5-6% CV) and capillary (21-25% CV) sites compared to ADVIA-2120 (0.6-2% CV). Other variables including blood collection tube manufacturer did not affect mean Hb content. These results demonstrate that even when most technical variables are minimized, the blood draw site and the analytical device can have a small but statistically significant effect on the mean and dispersion of Hb measurements. Even in this study, the few participants identified as mildly anemic using venous blood measured by ADVIA-2120 would not have been classified as anemic using their capillary blood samples or point-of-care analyzers. Thus, caution is warranted when comparing Hb values between studies having differences in blood draw site and Hb measuring device. Future anemia testing should maintain consistency in these analytical variables.

When Given a Fish vs. Offered a Fishing Rod: Outcomes of Receiving Contrasting Types of Help

In today’s fast paced, collaborative workplace cultures, it is hard for employees to not receive help and succeed alone. Thus, giving and receiving help is omnipresent in organizations. However, receiving help has proven to be both beneficial and harmful concurrently. To reconcile these conflicting outcomes of receiving help, we adopt a dual-type view of helping. We propose that employees who receive dependency help (i.e., where a leader fixes a problem on behalf of an employee or presents a final answer) report lower job satisfaction and higher turnover intention. In contrast, employees who receive autonomy help (i.e., where a leader guides an employee through the steps on how to fix a problem) report higher job satisfaction and lower turnover intention. We further invoke Self-Determination Theory to examine the mediating role of employees’ needs for perceived impact, sense of belongingness, and occupational self-efficacy in predicting divergent outcomes. Finally, we examine the moderating role of leader-employee relationships in elucidating how high-quality LMX can buffer against the negative effects of receiving dependency help. We test our hypotheses using a combination of experimental studies and a large field study comprising of 73 supervisors and 292 employees. In doing so, this research highlights how receiving contrasting types of help can yield divergent outcomes.

Cellular and Humoral Immunogenicity Investigation of Single and Repeated Allogeneic Tenogenic Primed Mesenchymal Stem Cell Treatments in Horses Suffering From Tendon Injuries.

The use of mesenchymal stem cells (MSCs) for the treatment of equine tendon disease is widely investigated because of their regenerative and immunomodulatory potential. However, questions have been raised concerning the immunogenic properties of allogeneic MSCs. Therefore, two studies were conducted to assess the safety of equine allogeneic peripheral blood-derived tenogenic primed MSCs (tpMSCs). The objective was to evaluate if a single and repeated tpMSC administration induced a cellular and humoral immune response in horses suffering from tendon injuries. Horses enrolled in the first study (n = 8) had a surgically induced superficial digital flexor tendon core lesion and were treated intralesionally with tpMSCs. Before and after treatment the cellular immunogenicity was assessed by modified mixed lymphocyte reactions. The humoral immune response was investigated using a crossmatch assay. Presence of anti-bovine serum albumin (BSA) antibodies was detected via ELISA. Horses enrolled in the second study (n = 6) suffered from a naturally occurring tendon injury and were treated twice with tpMSCs. Blood was collected after the second treatment for the same immunological assays. No cellular immune response was found in any of the horses. One out of eight horses in the first study and none of the horses in the second study had anti-tpMSC antibodies. This particular horse had an equine sarcoid and further investigation revealed presence of antibodies against sarcoid cells and epithelial-like stem cells before treatment, which increased after treatment. Additionally, formation of antibodies against BSA was observed. These findings might indicate a non-specific immune response generated after treatment. Serum from the other horses revealed no such antibody formation. These two studies showed that the administration of tpMSCs did not induce a cellular or humoral immune response following an intralesional single or repeated (two consecutive) allogeneic tpMSC treatment in horses with tendon injury, except for one horse. Therefore, a larger field study should confirm these findings and support the safe use of tpMSCs as a therapeutic for horses suffering from tendon injuries.

Variations in chemical defences and patterns of natural enemy attack between native and introduced populations of fireweed (Senecio madagascariensis): Implications for biological control

AbstractNative to South Africa, fireweed (Senecio madagascariensis Poiret; Asteraceae) is an annual or short‐lived perennial herb that is highly invasive in Australia, where it is a target for biological control. Preliminary research indicates that fireweed may be undergoing adaptive changes along its invasion gradient in Australia. Changes, such as a shift in plant chemical defence, may influence fireweed’s interaction with specialist insect herbivores, and hence the efficacy of a biocontrol program. As the testing of biocontrol candidates is a rigorous and lengthy process, confirmation of fireweed’s susceptibility to insect attack may assist in fast tracking candidate suitability, where changes in plant defensive chemistry are found. In a large experimental field study in fireweed’s native range, we compared the susceptibility of Australian and South African populations to insect natural enemy attack (in terms of plant biomass, insect richness and composition). Simultaneously, we assessed the variation in plant pyrrolizidine alkaloid concentrations and composition to rationalise any differences in natural enemy attack between populations. We found no significant differences in plant biomass across fireweed populations, despite significantly higher alkaloid concentrations in Australian populations. The composition of the endophagous (specialist) insect herbivore assemblage was related to alkaloid composition, but there were only minor differences in the assemblage between Australian and South African fireweed populations. Moreover, we found no significant differences in the abundance, richness or Shannon diversity indices of endophagous or ectophagous (generalist) insect herbivores between Australian and South African fireweed populations. Our results suggest that despite variations in chemical defence, Australian fireweed populations displayed neither reduced nor increased susceptibility to specialist insect herbivore attack, supporting the notion that if host‐specific biocontrol agents are released, their impact in Australia is unlikely to be influenced by any changes in plant chemical defence.

A large field study of relationship between indoor and outdoor climate in residential buildings

High-quality data on indoor climate and energy collected in buildings is required to deepen our understanding of building performance. The aim of this work was to investigate the relationship between the indoor and outdoor climate in Danish residential buildings. Field data was collected in 45 apartments from April 2019 to November 2020. Internet of things (IoT) devices were installed to record the temperature, relative humidity and CO2 concentration in the central corridor of each apartment. High CO2 concentration (above 1,000ppm) and overheating were observed in the apartments. The changeover between the heating mode and the free running mode occurred between 11.1 to 13.6°C of outdoor air temperature. The temperature setpoints of the heating systems were around 20.6-22.3°C, which could be useful values to feed building simulations in order to achieve more realistic predictions of indoor climate and energy. The results of this study improve our understanding of indoor environmental quality in residential buildings at a national level.

Complete Genome Sequencing of Field Isolates of Peste des Petits Ruminants Virus from Tanzania Revealed a High Nucleotide Identity with Lineage III PPR Viruses.

Simple SummaryPeste des petits ruminants virus (PPRV) causes a highly devastating disease, peste des petits ruminants (PPR), in sheep and goats, which is targeted for global control and eradication. However, in many developing countries, access to expensive sequencing technologies is limited and is compounded by difficulties in transporting clinical samples across international borders. Oxford nanopore MinION is a relatively cheap sequencing technology using portable devices that require minimal supporting laboratory infrastructure or technical expertise for sample preparation and rapid sequencing. In this study, Oxford nanopore MinION sequencing was carried out to generate complete genomes of PPRV from archived PPRV-positive samples collected from PPR outbreaks in goats in Ngorongoro and Momba districts in Tanzania during 2016 and 2018, respectively. Complete genomes of PPRV of 15,948 nucleotides long were generated within four hours of sequencing. The phylogenetic analysis of the complete genomes revealed a high nucleotide identity (96.19–99.24%) with lineage III PPR viruses currently circulating in East Africa, indicating a common origin. The Oxford nanopore MinION sequencer can be deployed to overcome diagnostic and surveillance challenges in developing countries in the PPR Global Control and Eradication program. However, the coverage depth was uneven across the genome and amplicon dropout was observed between the matrix (M) and fusion (F) genes. Thus, larger field studies are needed to allow the collection of sufficient data to assess the robustness of nanopore sequencing technology.Peste des petits ruminants virus (PPRV) causes a highly devastating disease of sheep and goats that threatens food security, small ruminant production and susceptible endangered wild ruminants. With policy directed towards achieving global PPR eradication, the establishment of cost-effective genomic surveillance tools is critical where PPR is endemic. Genomic data can provide sufficient in-depth information to identify the pockets of endemicity responsible for PPRV persistence and viral evolution, and direct an appropriate vaccination response. Yet, access to the required sequencing technology is low in resource-limited settings and is compounded by the difficulty of transporting clinical samples from wildlife across international borders due to the Convention on International Trade in Endangered Species (CITES) of Wild Fauna and Flora, and Nagoya Protocol regulations. Oxford nanopore MinION sequencing technology has recently demonstrated an extraordinary performance in the sequencing of PPRV due to its rapidity, utility in endemic countries and comparatively low cost per sample when compared to other whole-genome (WGS) sequencing platforms. In the present study, Oxford nanopore MinION sequencing was utilised to generate complete genomes of PPRV isolates collected from infected goats in Ngorongoro and Momba districts in the northern and southern highlands of Tanzania during 2016 and 2018, respectively. The tiling multiplex polymerase chain reaction (PCR) was carried out with twenty-five pairs of long-read primers. The resulting PCR amplicons were used for nanopore library preparation and sequencing. The analysis of output data was complete genomes of PPRV, produced within four hours of sequencing (accession numbers: MW960272 and MZ322753). Phylogenetic analysis of the complete genomes revealed a high nucleotide identity, between 96.19 and 99.24% with lineage III PPRV currently circulating in East Africa, indicating a common origin. The Oxford nanopore MinION sequencer can be deployed to overcome diagnostic and surveillance challenges in the PPR Global Control and Eradication program. However, the coverage depth was uneven across the genome and amplicon dropout was observed mainly in the GC-rich region between the matrix (M) and fusion (F) genes of PPRV. Thus, larger field studies are needed to allow the collection of sufficient data to assess the robustness of nanopore sequencing technology.

Controlling microbial activity on walls by a photocatalytic nanocomposite paint: A field study

Bacteria and fungi that grow on the walls can cause allergic reactions and infectious diseases in human. We proposed a low-cost and easy-to-operate testing protocol for large scale field studies to evaluate the long-term antimicrobial performance of a novel WOx paint in 2 primary schools. In Tun Mun and Tin Shui Wai schools, WOx paints were painted on semi-outdoor and indoor walls and daily chlorine disinfection was applied after class in TSW School. A guidance was proposed for the protocol using the ATP biofluorescence method for large-scale field studies. ATP swab samples were taken at locations with and without the WOx paint on a control basis with a sampling frequency once a week for three months. The ATP values were then processed and presented in box plots. In both schools, the median log-scale ATP values of walls with WOx paint were at least 0.5-log lower than those without WOx paint. The WOx paint also performed better than daily chlorine disinfection in reducing microbial activities in long-term. The proposed testing protocol is suitable to evaluate long-term performance of an antimicrobial paint by analyzing its microbial activity in large-scale field tests. The WOx paint shows long-term effectiveness in reducing microbial activities on wall surfaces in both indoor and semi-outdoor environments.