Large-scale Monitoring Studies Research Articles

Evaluation of microplastic pollution using bee colonies: An exploration of various sampling methodologies

Recent research has highlighted the potential of honeybees and bee products as biological samplers for monitoring xenobiotic pollutants. However, the effectiveness of these biological samplers in tracking microplastics (MPs) has not yet been explored. This study evaluates several methods of sampling MPs, using honeybees, pollen, and a novel in-hive passive sampler named the APITrap. The collected samples were characterized using a stereomicroscopy to count and categorise MPs by morphology, colour, and type. To chemical identification, a micro-Fourier transform-infrared (FTIR) spectroscopy was employed to determine the polymer types. The study was conducted across four consecutive surveillance programmes, in five different apiaries in Denmark. Our findings indicated that APITrap demonstrated better reproducibility, with a lower variation in results of 39%, compared to 111% for honeybee samples and 97% for pollen samples. Furthermore, the use of APITrap has no negative impact on bees and can be easily applied in successive samplings. The average number of MPs detected in the four monitoring studies ranged from 39 to 67 in the APITrap, 6 to 9 in honeybee samples, and 6 to 11 in pollen samples. Fibres were the most frequently found, accounting for an average of 91% of the total MPs detected in the APITrap, and similar values for fragments (5%) and films (4%). The MPs were predominantly coloured black, blue, green and red. Spectroscopy analysis confirmed the presence of up to five different synthetic polymers. Polyethylene terephthalate (PET) was the most common in case of fibres and similarly to polypropylene (PP), polyethylene (PE), polyacrylonitrile (PAN) and polyamide (PA) in non fibrous MPs. This study, based on citizen science and supported by beekeepers, highlights the potential of MPs to accumulate in beehives. It also shows that the APITrap provides a highly reliable and comprehensive approach for sampling in large-scale monitoring studies.

Exploring Influencing Factors and Innovative Solutions for Sustainable Water Management on Green Roofs: A Systematic Quantitative Review

Green roofs are becoming popular in urban areas due to their potential benefits, including energy efficiency, urban heat island mitigation, and stormwater management. However, their water consumption can negatively impact water resources. Therefore, carefully managing the water consumption of green roofs is crucial to ensure they do not exacerbate existing water scarcity issues. This review explores the influencing factors and innovative solutions that increase the sustainability of water management on green roofs. A systematic quantitative review was conducted on published studies on green roofs. The review highlighted that while small-scale experimental studies are almost saturated, large-scale monitoring studies are still lacking. Modelling and assessing green roof settings based on climatic conditions and water availability and consumption are essential for successful water management. Using integrated technologies and sensing systems can increase water management efficiency and sustainability. Rainwater may be sufficient as a water source for green roofs in wet climates, while irrigation is still needed in other climates. Phytoremediation and biosorption can potentially increase runoff water quality. Improving hydrological performance by increasing rainwater retention and reducing water consumption capacity can reduce demand for other water resources and effectively manage small storms, mitigating pressure on city infrastructure and increasing water quality. Seeking non-potable sources, such as greywater, or harvesting enough rainwater to be used for irrigation during dry weather periods is highly advantageous for improving the sustainability of green roofs.

Observational study of typhoon effects on the oval-shaped 330 m high Zhuhai Center Tower

This paper investigates typhoon-induced wind effects on a skyscraper based on 72-h-long field measurement records. In this large-scale monitoring study, the wind field characteristics surrounding the monitored building are first investigated using climate records collected by a moveable laser radar wind profiler that was temporarily fixed next to the tall building during Typhoon Cempaka, which could avoid adverse impacts caused by interference at the building top or the terrain effects through measurements from an anemometer atop the building or a meteorological observation tower that could be far away from the monitored building. Subsequently, the wind-excited cladding pressures and structural responses of the monitored skyscrapers are analysed according to measurements by pressure transducers, accelerometers and velocimeters, and the pressure distribution characteristics and structural dynamic properties are estimated by spectral analysis and the random decrement technique. Ultimately, provided the oval-shaped appearance and spiral configuration of the Zhuhai Center Tower, the results derived in this study are compared with those of other supertall buildings in terms of several aspects of concern, including the distribution features of wind pressures and acceleration response and amplitude-dependent characteristics of modal parameters, which would further the understanding of wind effects on tall buildings with various cross sections and building shapes. This study aims to provide comprehensive reference information for the wind-resistant design of high-rise buildings in tropical cyclone-prone regions.

Potassium carbonate (K2CO3) – A cheap, non-toxic and high-density floating solution for microplastic isolation from beach sediments

Beaches are good indicators for local microplastic distribution and pollution. Multiple methods have been developed for extracting microplastics from sediment through density separation. However, the chemicals applied are often expensive and harmful to the user or the environment. We briefly review the problems associated with the use of these chemicals and present a new floatation medium, potassium carbonate (K2CO3), that has many advantages over other available media. It is non-toxic and cheap, and with a density of 1.54 g/cm3 the K2CO3 solution yielded a mean recovery rate of around 90% for PVC, one of the densest polymers, that cannot be easily extracted with alternative floatation media. We propose that the use of K2CO3 is particularly promising for long term and large-scale monitoring studies, because it allows involving citizen scientists in such studies, leading to an increased public awareness of the plastic problem in the seas.

How many fish could be vocal? An estimation from a coral reef (Moorea Island)

A recurrent question arising in fish bioacoustics research concerns the number of vocal fish species that may exist. Although it is not possible to provide a precise globally valid number, an estimation based on recordings already collected at coral reefs (Moorea) and on morphological approaches indicates that approximately half of the fish families of this particular environment has at least one known sound-producing species. In light of this, acoustic behaviour should be fully considered in biology, ecology and management plans as it may provide information on a consistent portion of fish biodiversity. Fish bioacoustics has switched from anecdotal reports to long-term, large-scale monitoring studies, capable of providing high resolution information on fish populations’ composition and dynamics. This information is vital for successful management plans in our quickly changing seas.

Monitoring serologic response to single in ovo vaccination with an immune complex vaccine against infectious bursal disease in broilers

The infectious bursal disease (IBD) virus is one of the most resistant and prevalent virus worldwide in the poultry industry, being vaccination the main tool to control the disease. For this reason, consistent and uniform immunization of broiler flocks against IBD is necessary to avoid the disease spreading. The aim of this study was to apply and assess an epidemiologic mapping tool focused on the immunization by in ovo single broiler vaccination using an immune complex IBD vaccine. With this regard, 7,576 serum samples were collected from 603 broiler flocks raised in 354 Spanish farms. To do so, blood samples were randomly collected from birds with ages between 35 to 51 d, and the serum was analyzed by ELISA. The results obtained from this study suggested a high uniform immunization against IBDV and a protective immunization between 35 and 51 d of age, with mean titer values ranging between 6,331 and 7,426. In addition, seroprevalence titer data of this large-scale monitoring study fitted a polynomial equation with a R2 value of 0.77, helping to explain and predict the humoral response to IBD vaccination. This seroprevalence map was applied to broiler production and was based on business intelligence tool that incorporates newly developed mapping tool to cover the need of having real-time information of humoral response to IBD vaccination and could be an effective tool for veterinary services to control and prevent IBD.

A Fast and Easily Parallelizable Biosensor Method for Measuring Extractable Tetracyclines in Soils.

Quantification of extractable antibiotics in soils is important to assessing their bioavailability and mobility, and ultimately their ecotoxicological and health risks. This study aimed to establish a biosensor method for detecting extractable tetracyclines in soils (Alfisol, Mollisol, and Ultisol) using whole-cell biosensors containing a reporter plasmid (pMTGFP or pMTmCherry) carrying fluorescent protein genes tightly controlled by tetracyclines-responsive control region (tetRO). This whole-cell biosensor method can simultaneously measure 96 or more samples within 6 h and is easily parallelizable, whereas a typical high-performance liquid chromatography (HPLC) method may require 7 times more of analysis time and much greater cost to achieve similar analytical throughput. The biosensor method had a detection limit for each of six tetracyclines between 5.32-10.2 μg/kg soil, which is considered adequate for detecting tetracyclines in ethylenediaminetetraacetic acid (EDTA) extracts of soils. Relative standard deviation was between 19.8-51.2% for the biosensor Escherichia coli DH5α/pMTGFP and 2.98-25.8% for E. coli DH5α/pMTmCherry, respectively, suggesting that E. coli DH5α/pMTmCherry was superior to E. coli DH5α/pMTGFP for detecting extractable tetracyclines in soils. This new, fast, easily parallelizable, and cost-effective biosensor method has the potential for measuring extractable concentrations of tetracyclines for a large number of soil samples in large-scale monitoring studies.

Monitoring of pesticides water pollution-The Egyptian River Nile.

BackgroundPersistent organic pollutants represent about 95 % of the industrial sector effluents in Egypt. Contamination of the River Nile water with various pesticides poses a hazardous risk to both human and environmental compartments. Therefore, a large scale monitoring study was carried on pesticides pollution in three geographical main regions along the River Nil water stream, Egypt.MethodsOrganochlorine and organophosphorus pesticides were extracted by liquid-liquid extraction and analyzed by GC-ECD.ResultsOrganochlorine pesticides mean concentrations along the River Nile water samples were 0.403, 1.081, 1.209, 3.22, and 1.192 μg L−1 for endrin, dieldrin, p, p’-DDD, p, p’-DDT, and p, p’-DDE, respectively. Dieldrin, p, p’-DDT, and p, p’-DDE were above the standard guidelines of the World Health Organization. Detected organophosphorus pesticides were Triazophos (2.601 μg L−1), Quinalphos (1.91 μg L−1), fenitrothion (1.222 μg L−1), Ethoprophos (1.076 μg L−1), chlorpyrifos (0.578 μg L−1), ethion (0.263 μg L−1), Fenamiphos (0.111 μg L−1), and pirimiphos-methyl (0.04 μg L−1). Toxicity characterization of organophosphorus pesticides according to water quality guidelines indicated the hazardous risk of detected chemicals to the public and to the different environmental compartments. The spatial distribution patterns of detected pesticides reflected the reverse relationship between regional temperature and organochlorine pesticides distribution. However, organophosphorus was distributed according to the local inputs of pollutant compounds.ConclusionsToxicological and water quality standards data revealed the hazardous risk of detected pesticides in the Egyptian River Nile water to human and aquatic life. Thus, our monitoring data will provide viewpoints by which stricter legislation and regulatory controls can be admitted to avoid River Nile pesticide water pollution.

Large-scale monitoring of effects of clothianidin dressed oilseed rape seeds on pollinating insects in Northern Germany: implementation of the monitoring project and its representativeness.

Monitoring studies at the landscape level are complex, expensive and difficult to conduct. Many aspects have to be considered to avoid confounding effects which is probably the reason why they are not regularly performed in the context of risk assessments of plant protection products to pollinating insects. However, if conducted appropriately their contribution is most valuable. In this paper we identify the requirements of a large-scale monitoring study for the assessment of side-effects of clothianidin seed-treated winter oilseed rape on three species of pollinating insects (Apis mellifera, Bombus terrestris and Osmia bicornis) and present how these requirements were implemented. Two circular study sites were delineated next to each other in northeast Germany and comprised almost 65 km2 each. At the reference site, study fields were drilled with clothianidin-free OSR seeds while at the test site the oilseed rape seeds contained a coating with 10 g clothianidin and 2 g beta-cyfluthrin per kg seeds (Elado®). The comparison of environmental conditions at the study sites indicated that they are as similar as possible in terms of climate, soil, land use, history and current practice of agriculture as well as in availability of oilseed rape and non-crop bee forage. Accordingly, local environmental conditions were considered not to have had any confounding effect on the results of the monitoring of the bee species. Furthermore, the study area was found to be representative for other oilseed rape cultivation regions in Europe.

THE PRESENCE OF BENEFICIAL ARTHROPODS IN ORGANIC VERSUS IPM PEAR ORCHARDS AND THEIR ABILITY TO PREDATE PEAR SUCKERS (CACOPSYLLA PYRI)

In Belgium, two cultivation methods of pear are widely applied, namely organic farming and integrated pest management (IPM). Organic pear orchards are characterized by the use of green techniques such as natural fertilizers and the restricted use of crop protection products, whereas integrated pest management combines a well-tuned integration of biological pest control and crop protection treatments. It is generally considered that these two cultivation methods have different effects on the density of beneficial arthropods in the orchard and the occurrence of common pests as well. Therefore, a large-scale monitoring study was executed comprising extensive samplings throughout the year in 14 pear orchards and comparing beneficial fauna population developments between organic and IPM pear orchards. Pear sucker (Cacopsylla sp. or pear psylla) which is considered the major pest of pear in Belgium, was monitored as well. Organic orchards clearly displayed a lower pear psylla pressure correlating with higher proportions of specific predator populations, particularly velvet mites (Trombidiidae) and spiders (Araneae). IPM orchards showed higher numbers of Anthocoris spp. in summer. In addition, predation of C. pyri was verified by PCR-based gut content analysis. Regarding the results of this prey-predator PCR, Anthocoridae had the highest chance for detection of psyllid DNA in their gut. Velvet mites also scored particularly well for the PCR detections, indicating that other arthropods besides Anthocoridae could have an impact on the psyllid pressure in a pear orchard.

Preliminary estimation of deoxynivalenol excretion through a 24 h pilot study.

A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid phase extraction and gas chromatography determination coupled to mass spectrometry in tandem. Satisfactory results in method performance were achieved. The method’s accuracy was in a range of 68%–108%, with intra-day relative standard deviation and inter-day relative standard deviation lower than 12% and 15%, respectively. The limits of quantitation ranged from 0.1 to 8 µg/Kg. The matrix effect was evaluated and matrix-matched calibrations were used for quantitation. Only deoxynivalenol (DON) was quantified in both food and urine samples. A total DON daily intake amounted to 49.2 ± 5.6 µg whereas DON daily excretion of 35.2 ± 4.3 µg was determined. DON daily intake represented 68.3% of the established DON provisional maximum tolerable daily intake (PMTDI). Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies.

Radioactivity of Drinking-Water in the Vicinity of Nuclear Power Plants in China Based on a Large-Scale Monitoring Study

The public concern for radioactivity of drinking-water has been increasing in recent years after the rapid development of nuclear power plants, and especially after the Fukushima nuclear accident. In this study, the radioactivity of water samples collected in the vicinity of nuclear facilities from seven provinces in China was measured and an average annual equivalent effective dose derived from drinking-water ingestion was calculated. The results showed that, in winter and spring, the activities of gross α and β ranged from 0.009 Bq/L to 0.200 Bq/L and from 0.067 Bq/L to 0.320 Bq/L, respectively. While, in summer and autumn, the activities of gross α and β varied from 0.002 Bq/L to 0.175 Bq/L and from 0.060 Bq/L to 0.334 Bq/L. Our results indicated that the gross α and β activities in these measured water samples were below the WHO recommended values (0.5 Bq/L for gross α and 1.0 Bq/L for gross β) and the annual equivalent effective dose derived from drinking-water ingestion was at a safe level.

Polychaete Richness and Abundance Enhanced in Anthropogenically Modified Estuaries Despite High Concentrations of Toxic Contaminants

Ecological communities are increasingly exposed to multiple chemical and physical stressors, but distinguishing anthropogenic impacts from other environmental drivers remains challenging. Rarely are multiple stressors investigated in replicated studies over large spatial scales (>1000 kms) or supported with manipulations that are necessary to interpret ecological patterns. We measured the composition of sediment infaunal communities in relation to anthropogenic and natural stressors at multiple sites within seven estuaries. We observed increases in the richness and abundance of polychaete worms in heavily modified estuaries with severe metal contamination, but no changes in the diversity or abundance of other taxa. Estuaries in which toxic contaminants were elevated also showed evidence of organic enrichment. We hypothesised that the observed response of polychaetes was not a ‘positive’ response to toxic contamination or a reduction in biotic competition, but due to high levels of nutrients in heavily modified estuaries driving productivity in the water column and enriching the sediment over large spatial scales. We deployed defaunated field-collected sediments from the surveyed estuaries in a small scale experiment, but observed no effects of sediment characteristics (toxic or enriching). Furthermore, invertebrate recruitment instead reflected the low diversity and abundance observed during field surveys of this relatively ‘pristine’ estuary. This suggests that differences observed in the survey are not a direct consequence of sediment characteristics (even severe metal contamination) but are related to parameters that covary with estuary modification such as enhanced productivity from nutrient inputs and the diversity of the local species pool. This has implications for the interpretation of diversity measures in large-scale monitoring studies in which the observed patterns may be strongly influenced by many factors that covary with anthropogenic modification.

Can starling eggs be useful as a biomonitoring tool to study organohalogenated contaminants on a worldwide scale?

Large-scale international monitoring studies are important to assess emission patterns and environmental distributions of organohalogenated contaminants (OHCs) on a worldwide scale. In this study, the presence of OHCs was investigated on three continents (Europe, North America and Australasia), using eggs of starlings (Sturnus vulgaris and Sturnus unicolor) to assess their suitability for large-scale monitoring studies. To the best of our knowledge, this is the first study using bird eggs of the same species as a biomonitor for OHCs on an intercontinental scale. We found significant differences in OHC concentrations of the eggs among sampling locations, except for hexachlorocyclohexanes (HCHs). Mean concentrations of sum polychlorinated biphenyls (PCBs) in eggs ranged from 78±26ng/glipid weight (lw) in Australia to 2900±1300ng/glw in the United States. The PCB profile was dominated by CB 153 and CB 138 in all locations, except for New Zealand, where the contribution of CB 95, CB 101 and CB 149 was also high. The highest mean sum polybrominated diphenyl ether (PBDE) concentrations were found in Canada (4400±830ng/glw), while the lowest mean PBDE concentrations were measured in Spain (3.7±0.1ng/glw). The PBDE profile in starling eggs was dominated by BDE 47 and BDE 99 in all countries, but in Belgium, the higher brominated PBDEs had a higher contribution compared to other countries. For the organochlorine pesticides (OCPs), dichlorodiphenyltrichloroethanes (DDTs) ranged from 110±16ng/glw in France to 17,000±3400ng/glw in New Zealand, while HCHs and hexachlorobenzene were generally in low concentrations in all sampling locations. Chlordanes were remarkably high in eggs from the United States (2500±1300ng/glw). The OCP profile in all countries was largely dominated by p,p′-DDE. In general, the worldwide trends we observed in starling eggs were in accordance with the literature on human and environmental OHC data, which suggests that there is potential for using starling eggs as a biomonitoring tool on a large geographical scale.

A Whale Tale: Using Blubber Biopsies to Characterize Pacific Ocean Pollutant Trends

Vol. 119, No. 3 NewsOpen AccessA Whale Tale: Using Blubber Biopsies to Characterize Pacific Ocean Pollutant Trends Angela Spivey Angela Spivey Search for more papers by this author Published:1 March 2011https://doi.org/10.1289/ehp.119-a133bAboutSectionsPDF ToolsDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InReddit Expression of the enzyme CYP1A1 in the skin of marine mammals has been shown by multiple studies to indicate exposure to organic pollutants in a dose-dependent manner. A new large-scale monitoring study investigated whether analysis of dermal CYP1A1 expression and organic pollutants in sperm whales (Physeter macrocephalus) could reveal oceanwide geographical trends in chemical exposure [EHP119(3):337–343; Godard-Codding et al]. This is the first known study to assess broad geographic trends in CYP1A1 expression, stable carbon and nitrogen isotopes, and organic pollutant burdens in a threatened whale species.The authors used immunochemistry to analyze CYP1A1 expression in skin and blubber samples collected from 234 sperm whales from five Pacific Ocean regions. Variation in the whales’ trophic level (position in the food chain) was examined by using mass spectrometry to measure nitrogen isotopes in skin samples; enrichment of an animal’s tissue nitrogen is known to occur as the animal eats higher on the food chain. The general latitude frequented by the whales—a reflection of where the whales were likely to have been exposed to pollution—was determined by analyzing carbon isotope ratios.The whales exhibited significant regional differences in CYP1A1 expression. Expression was highest among whales from the Galapagos Islands, a United Nations World Heritage marine reserve, and lowest among whales from sites farthest away from continents. Differences in the whales’ age, sex, and diet did not appear to explain regional differences but could not be ruled out unequivocally.This study did not show a significant correlation between CYP1A1 expression in skin cells and actual pollutant burden in blubber, as measured by analyzing eight sex-specific pooled samples for burdens of polycyclic aromatic hydrocarbons, hexachlorobenzene, polychlorinated biphenyls, and the pesticide DDT, then comparing them with CYP1A1 immunohistochemistry scores estimated for the pooled samples.However, the small size of the individual biopsies allowed under standards for humane biopsying of marine mammals prevented detailed chemical analyses and limited the power to detect significant associations. Also, the biopsies were limited to the outer blubber layer, which is less metabolically active than deeper tissue. Studies in bottlenose dolphins have shown that CYP1A1 expression in the skin is more strongly related to pollutants measured in deeper blubber than in blubber closer to the skin surface; whether such stratification happens in other cetaceans requires further study.The study succeeded at identifying regional differences in CYP1A1 expression, providing a baseline for this known biomarker of exposure to organic pollutants. Future studies that profile CYP1A1 expression in cetacean skin biopsies oceanwide are warranted to explore the global distribution of biochemically relevant levels of these chemicals.Carnivorous sperm whales are at the top of their food chain.FiguresReferencesRelatedDetails Vol. 119, No. 3 March 2011Metrics About Article Metrics Publication History Originally published1 March 2011Published in print1 March 2011 Financial disclosuresPDF download License information EHP is an open-access journal published with support from the National Institute of Environmental Health Sciences, National Institutes of Health. All content is public domain unless otherwise noted. Note to readers with disabilities EHP strives to ensure that all journal content is accessible to all readers. However, some figures and Supplemental Material published in EHP articles may not conform to 508 standards due to the complexity of the information being presented. If you need assistance accessing journal content, please contact [email protected]. Our staff will work with you to assess and meet your accessibility needs within 3 working days.

Decrease in air pollution load in urban environment of Bratislava (Slovakia) inferred from accumulation of metal elements in lichens

The study illustrates the response of epiphytic lichens to changing atmospheric conditions in Central Europe, where the emission of air pollutants has significantly decreased from 1990, in the area in and around Bratislava City. Variation in concentrations of seven metal elements (Cu, Cd, Cr, Mn, Ni, Pb and Zn) in the thalli of Evernia prunastri, Hypogymnia physodes and Parmelia sulcata is assessed. Samples of these species were exposed in lichen bags in 39 sites throughout the territory of the city (more than 300 km(2)) during the period December 2006-February 2007. The samples were analyzed by AAS for metal element contents prior to and after exposure. The decrease in air pollution (for all studied elements by more than 90%) corresponded to a decrease in the accumulation of elements in lichen thalli, e.g. the contents of Pb decreased by 69% and of Cd by 34% on average. The results show also variations in accumulation between with different lichen species. The background values of metal element contents in thalli of H. physodes growing in situ were measured in semi-natural sites in Slovakia. It is suggested that these can be used as a reference in large-scale monitoring studies in Central Europe. Analysis of compatible data from the current study, and the study performed at the end of 1990s shows a significant decrease of metal elements in the air pollution load.

Pacific Ocean–Wide Profile of CYP1A1 Expression, Stable Carbon and Nitrogen Isotope Ratios, and Organic Contaminant Burden in Sperm Whale Skin Biopsies

BackgroundOcean pollution affects marine organisms and ecosystems as well as humans. The International Oceanographic Commission recommends ocean health monitoring programs to investigate the presence of marine contaminants and the health of threatened species and the use of multiple and early-warning biomarker approaches.ObjectiveWe explored the hypothesis that biomarker and contaminant analyses in skin biopsies of the threatened sperm whale (Physeter macrocephalus) could reveal geographical trends in exposure on an oceanwide scale.MethodsWe analyzed cytochrome P450 1A1 (CYP1A1) expression (by immunohistochemistry), stable nitrogen and carbon isotope ratios (as general indicators of trophic position and latitude, respectively), and contaminant burdens in skin biopsies to explore regional trends in the Pacific Ocean.ResultsBiomarker analyses revealed significant regional differences within the Pacific Ocean. CYP1A1 expression was highest in whales from the Galapagos, a United Nations Educational, Scientific, and Cultural Organization World Heritage marine reserve, and was lowest in the sampling sites farthest away from continents. We examined the possible influence of the whales’ sex, diet, or range and other parameters on regional variation in CYP1A1 expression, but data were inconclusive. In general, CYP1A1 expression was not significantly correlated with contaminant burdens in blubber. However, small sample sizes precluded detailed chemical analyses, and power to detect significant associations was limited.ConclusionsOur large-scale monitoring study was successful at identifying regional differences in CYP1A1 expression, providing a baseline for this known biomarker of exposure to aryl hydrocarbon receptor agonists. However, we could not identify factors that explained this variation. Future oceanwide CYP1A1 expression profiles in cetacean skin biopsies are warranted and could reveal whether globally distributed chemicals occur at biochemically relevant concentrations on a global basis, which may provide a measure of ocean integrity.

Urban Forest Health Monitoring: Large-Scale Assessments in the United States

The U.S. Department of Agriculture, Forest Service (USFS), together with state partners, developed methods to monitor urban forest structure, function, and health at a large statewide scale. Pilot studies have been established in five states using protocols based on USFS Forest Inventory and Analysis and Forest Health Monitoring program data collection standards. Variables and data analysis are described. Advantages of a large-scale monitoring study are discussed and examples of results from Wisconsin are presented. Studies in Indiana, Wisconsin, New Jersey, Tennessee, and Colorado, U.S., have shown that urban forest health monitoring data collection and analysis is feasible and can be implemented nationally.

Nitrogen Deposition and the Biodiversity of Boreal Forests: Implications for the Nitrogen Critical Load

The critical load concept is used to establish the deposition levels which ecosystems can tolerate without significant harmful effects. Here we summarize work within the Swedish research program Abatement Strategies for Transboundary Air Pollution (ASTA) assessing the critical load of N for boreal forests. Results from both field experiments in an area with low background N deposition in northern Sweden, and from a large-scale monitoring study, show that important vegetational changes start to take place when adding low N doses and that recovery of the vegetation after ceasing N input is a very slow process. The data presented indicate that changes in key ecosystem components occur even at a lower rate of N input than the present recommended empirical critical load for boreal forest understorey vegetation of 10-15 kg N ha(-1) yr(-1). Based on the data presented, we suggest that the critical load should be lowered to 6 kg N ha(-1) yr(-1).

Landscape-level approach to assess aquatic exposure via spray drift for pesticides: a case study in a Mediterranean area.

The development of methods to extract information from landscape analysis to refine risk assessment is becoming increasingly important. This paper presents results from a pesticide surface water exposure assessment at the watershed scale, based on a combination of edge of field studies, large-scale monitoring studies, and modeling activities with GIS-based landscape analysis methodologies covering an area of approximately 3200 ha surrounding the Simeto River in Sicily (Italy). The dynamic behavior of the pesticide chlorpyrifos-methyl was modeled in two different steps: calculation of the fraction of the application rate that is deposited beyond the field edge and simulation of the fate and persistence of the pesticide in the aquatic environment. Drift loads showed high spatial variability. Considering spray drift deposition as a fraction of the pesticide application rate, 60% of the results were < or = 0.02 (equal to 0.04 mg/m2). Only 8.5% of the results were above 0.5. The highly variability of the landscape factors was reflected in the results. More than 60% of the predicted pesticide concentrations were less than the limit of quantification (0.05 microg/L), affecting about 75% of the total length of the river tract analyzed. Predicted pesticide concentrations were higher than 0.1 microg/L in 23% of cases, but this corresponded to an insignificant portion of the river (1.2% of the total length). These results suggest that management options, such as increased no-spray zones, could provide further protection for surface water. These could be modeled to illustrate their overall impact. As an alternative, the introduction of a 20-m no-spray zone clearly reduced potential exposure, and 92% of the water body was protected. Estimated data are in agreement with data collected during a field monitoring study.