Large-scale Expansion Research Articles

고구려 갑주 연구의 다각화를 위한 시

According to “The Chronicles of the Three States”, infantry and cavalry was used in Gogurye, so-called a “brave cavalry”, an “elite cavalry” or an “iron cavalry” was its core. Armours were mass produced, and infantry and cavalry was in armour in concert with cavalry tactics, large-scale military activities and expansion in the 4th century. The study about an armour of Gogurye was begun with analysis of an armour of a mural. After 1970s, a lot of armour was found in tombs, castles, architecture remains and bastions. After 1990s, studies based on excavated real armour materials were progressed, and the system, the composition and the overall flow of armour came to comprehend. The armour of Gogurye is distributed in Jian-Huanren area, Hun River area, north-western area of Korea, Seoul and Northern area of Gyeonggi. A lamellar was buried in a stone mound tomb in Jian. A various -shaped lamellar was used, and a gilt-bronze lamellar was also produced. Amours excavated in Dongtaizi site, castles and other area belongs to the 6th century, and the trapezoid-shaped lamellar was main. A gilt-bronze lamellar, the change of a lamellar by bending and bevel cut technique, lamellars found in No.26 site of Wunu Castle, a strange shaped lamellar and a helmet of Longori castle was analyzed to diversify research about the armour of Gogurye. At present, the armour excavated in castle is not analyzed yet. As composing and studying armours of stone mound tombs, architecture remains, bastions as well as castles, we can understand the whole aspect of armours of Gogurye from the 4th to the 7th century exactly.

The genome of Acorus deciphers insights into early monocot evolution

Acorales is the sister lineage to all the other extant monocot plants. Genomic resource enhancement of this genus can help to reveal early monocot genomic architecture and evolution. Here, we assemble the genome of Acorus gramineus and reveal that it has ~45% fewer genes than the majority of monocots, although they have similar genome size. Phylogenetic analyses based on both chloroplast and nuclear genes consistently support that A. gramineus is the sister to the remaining monocots. In addition, we assemble a 2.2 Mb mitochondrial genome and observe many genes exhibit higher mutation rates than that of most angiosperms, which could be the reason leading to the controversies of nuclear genes- and mitochondrial genes-based phylogenetic trees existing in the literature. Further, Acorales did not experience tau (τ) whole-genome duplication, unlike majority of monocot clades, and no large-scale gene expansion is observed. Moreover, we identify gene contractions and expansions likely linking to plant architecture, stress resistance, light harvesting, and essential oil metabolism. These findings shed light on the evolution of early monocots and genomic footprints of wetland plant adaptations.

Preparation of thermosensitive PNIPAm- based copolymers with tertiary amino and hydroxyl monomer for cell attachment and detachment study

Thermo-responsive microcarriers are essential for large-scale expansion of anchorage-dependent cells in a suspension culture. In this study, the authors prepared four different N-isopropylacrylamide (NIPAm)-based thermosensitive copolymers by copolymerizing with N-hydroxyethyl acrylamide (HEAA), 2-Hydroxyethyl methacrylate (HEMA), 2-(Diethylamino) ethylmethacrylate (DEAEMA), and 2-(Dimethylamino) ethylmethacylate (DMAEMA) through free radical polymerization (FRP) and used them for surface modification of Cytodex-3 microcarrier. The thermosensitive characteristic of the copolymers enabled microcarriers to attach and detach cells in response to temperature changes. Herein, NIH 3T3 cells were used as a model cell line and it was found that the cells' adhesion rate on Cytodex-3 microcarriers modified with different formulations of thermosensitive copolymers was greater than unmodified microcarriers. The microcarriers coated with 5 wt% of the copolymer showed better cell attachment than 3 wt% and 10 wt% copolymer coated microcarriers. Particularly, 5 wt% P(NIPAm-5% HEMA) copolymer coated microcarriers showed excellent cell attachment than other formulations. More importantly, as the temperature was reduced to 4 °C, the cells cultured on copolymer modified microcarriers were completely detached as a cell sheet from it withoutproteolytic enzymetreatment. Besides preserving the functionality and integrity of cellular structures, the thermal-induced detachment of cells from modified microcarriers was much higher (∼9.9 x 104 cells/mL) than cells detached from the original Cytodex-3 (∼7.5x103 cells/mL) in 1 h at 4 °C. Therefore, this study verified that microcarriers modified with thermosensitive copolymers exhibited enhanced cell attachment and detachment with temperature change, while maintaining cell functionality, thereby providing adequate number of viable cells for large scale production.

Comparison of the different anti-CD16 antibody clones in the activation and expansion of peripheral blood NK cells

Natural killer (NK) cells are promising tool for cancer treatment. Methods have been developed for large-scale NK cell expansion, including feeder cell-based methods or methods involving stimulation with NK cell activating signals, such as anti-CD16 antibodies. Different clones of anti-CD16 antibodies are available; however, a comprehensive comparison of their differential effects on inducing NK cell activation and expansion has not been conducted among these various clones under the same experimental conditions. Herein, we found that the NK cell expansion rate differed depending on the various anti-CD16 antibodies (CB16, 3G8, B73.1, and MEM-154) coated on microbeads when stimulated with genetically engineered feeder cells, K562‑membrane-bound IL‑18, and mbIL‑21 (K562‑mbIL‑18/-21). Only the CB16 clone combination caused enhanced NK cell expansion over K562‑mbIL‑18/-21 stimulation alone with similar NK cell functionality. Treatment with the CB16 clone once on the initial day of NK cell expansion was sufficient to maximize the combination effect. Overall, we developed a more enhanced NK expansion system by merging a feeder to effectively stimulate CD16 with the CB16 clone.

Anterograde Collisional Analysis of Solar Wind Ions

Owing to its low density and high temperature, the solar wind frequently exhibits strong departures from local thermodynamic equilibrium, which include distinct temperatures for its constituent ions. Prior studies have found that the ratio of the temperatures of the two most abundant ions—protons (ionized hydrogen) and α-particles (ionized helium)—is strongly correlated with the Coulomb collisional age. These previous studies, though, have been largely limited to using observations from single missions. In contrast, this present study utilizes contemporaneous, in situ observations from two different spacecraft at two different distances from the Sun: the Parker Solar Probe (PSP; r = 0.1–0.3 au) and Wind (r = 1.0 au). Collisional analysis, which incorporates the equations of collisional relaxation and large-scale expansion, was applied to each PSP datum to predict the state of the plasma farther from the Sun at r = 1.0 au. The distribution of these predicted α–proton relative temperatures agrees well with that of values observed by Wind. These results strongly suggest that, outside of the corona, relative ion temperatures are principally affected by Coulomb collisions and that the preferential heating of α-particles is largely limited to the corona.

The effect of dorsal column lesions in the primary somatosensory cortex and medulla of adult rats

Spinal cord injury is a devastating condition that haunts human lives. Typically, patients experience referred phantom sensations on the hand when they are touched on the face. In adult monkeys, massive deafferentations such as chronic dorsal column lesions at higher cervical levels result in the large-scale expansion of face inputs into the deafferented hand cortex of area 3b. However, adult rats with thoracic dorsal column lesions do not demonstrate such large-scale reorganization. The large-scale face expansion in area 3b of monkeys is driven by the reorganization of the cuneate nucleus in the medulla. The sprouting of afferents from the trigeminal nucleus to the adjacent deafferented cuneate nucleus is facilitated by close proximity and compactness of the medulla in primates. Previously, in adult rats with thoracic lesions, the cuneate nucleus was not deafferented and its functional organization was not explored. The extent of the deafferentation and the duration of the recovery period are two major factors that determine the extent of reorganization. Hence, higher cervical (C3-C4) dorsal column lesions were performed, which cause massive deafferentations, and physiological maps were obtained after prolonged recovery periods (3 weeks -18 months). In spite of the above, the expansion of the intact face inputs was not observed in the deafferented zones of the primary somatosensory cortex (SI) and medulla of adult rats. The deafferented forelimb and hindlimb representations in SI were unresponsive to cutaneous stimulation of any part of the body. The cuneate and gracile nuclei in rats with complete dorsal column lesions remained mostly inactive except for a few sites which responded to stimulation of the spared upper arm. Hence, dorsal column lesions have different effects on the adult primate and rodent somatosensory systems. Appreciating this inter-species difference can aid in identifying the underlying neural substrates and restrict maladaptive reorganizations to cure phantom sensations.

Suspension culture on microcarriers and as aggregates enables expansion and differentiation of pluripotent stem cells (PSCs)

Background aimsHuman pluripotent stem cells (PSCs) hold a great promise for promoting regenerative medical therapies due to their ability to generate multiple mature cell types and for their high expansion potential. However, cell therapies require large numbers of cells to achieve desired therapeutic effects, and traditional two-dimensional static culture methods cannot meet the required production demand for cellular therapies. One solution to this problem is scaling up expansion of PSCs in bioreactors using culture strategies such as growing cells on microcarriers or as aggregates in suspension culture. MethodsIn this study, we directly compared PSC expansion and quality parameters in microcarrier- and aggregate-cultures grown in single-use vertical-wheel bioreactors. ResultsWe showed comparable expansion of cells on microcarriers and as aggregates by day 6 with a cell density reaching 2.2 × 106 cells/mL and 1.8 × 106 cells/mL and a fold-expansion of 22- and 18-fold, respectively. PSCs cultured on microcarriers and as aggregates were comparable with parallel two-dimensional cultures and with each other in terms of pluripotency marker expression and retention of other pluripotency characteristics as well as differentiation potential into three germ layers, neural precursor cells and cardiomyocytes. ConclusionsOur study did not demonstrate a clear advantage between the two three-dimensional methods for the quality parameters assessed. This analysis adds support to the use of bioreactor systems for large scale expansion of PSCs, demonstrating that the cells retain key characteristics of PSCs and differentiation potential in suspension culture.

Research on the Influence of Teaching Quality on Employment Quality of Graduates in Higher Vocational Colleges Based on Human Capital Effect

Against the backdrop of large-scale enrollment expansion, higher vocational education in China is facing a new task of achieving an "increase in quantity and quality" in the cultivation of technical and skilled talents, and promoting fuller and higher quality employment for vocational college graduates. On the basis of reviewing and organizing relevant literature, this study constructs a conceptual model of the relationship between teaching quality, graduate human capital, and graduate employment quality in vocational colleges, and proposes relevant research hypotheses. Through a questionnaire survey of some vocational colleges in Chongqing, a total of 520 valid data were obtained, and the research hypotheses were validated using SPSS and AMOS software for reliability and validity testing, descriptive statistical analysis, correlation analysis, and structural equation modeling analysis. Research has found that the teaching quality of vocational colleges has a significant positive impact on employment quality, and human capital also has a significant positive impact on employment quality. Teaching quality also has a significant positive impact on human capital; Human capital plays a partial mediating role between the quality of teaching and the quality of employment. The research and analysis results indicate that vocational colleges should fully consider students' career development, reshape their value orientation, position industry needs, adjust professional settings, innovate teaching methods, promote curriculum system reform, strengthen the construction of teachers and teaching resources, collaborate with the main body to build a teaching quality assurance system, improve students' satisfaction with higher education and teaching work, improve teaching quality, and lay the foundation for better achieving high-quality employment.

Large-scale expansions of Friedreich's ataxia GAA•TTC repeats in an experimental human system: role of DNA replication and prevention by LNA-DNA oligonucleotides and PNA oligomers.

Friedreich's ataxia (FRDA) is caused by expansions of GAA•TTC repeats in the first intron of the human FXN gene that occurduring both intergenerational transmissions and in somatic cells. Here we describe an experimental system to analyze large-scale repeat expansions in cultured human cells. It employs a shuttle plasmid that can replicate from the SV40 origin in human cells or be stably maintained in S. cerevisiae utilizing ARS4-CEN6. It also contains a selectable cassette allowing us to detect repeat expansions that accumulated in human cells upon plasmid transformation into yeast. We indeed observed massive expansions of GAA•TTC repeats, making it the first genetically tractable experimental system to study large-scale repeat expansions in human cells. Further, GAA•TTC repeats stall replication fork progression, while the frequency of repeat expansions appears to depend on proteins implicated in replication fork stalling, reversal, and restart. Locked nucleic acid (LNA)-DNA mixmer oligonucleotides and peptide nucleic acid (PNA) oligomers, which interfere with triplex formation at GAA•TTC repeats in vitro, prevented the expansion of these repeats in human cells. We hypothesize, therefore, that triplex formation by GAA•TTC repeats stall replication fork progression, ultimately leading to repeat expansions during replication fork restart.

Machine Learning Cosmic Backreaction and Its Effects on Observations.

Symbolic expressions for cosmic backreaction and mean redshift drift in a range of two-region models in terms of average quantities are presented. The demonstration that these expressions can be obtained constitutes the opening of a new avenue towards understanding the effects of cosmic backreaction in our universe: With a symbolic expression for the redshift drift at hand, the redshift drift can be used to constrain cosmological parameters including the large-scale expansion rate and backreaction. In addition, by introducing symbolic expressions for cosmic backreaction, this quantity can be constrained with observations such as redshift-distance measures.

Genome-Wide Analysis and Functional Characterization of LACS Gene Family Associated with Lipid Synthesis in Cotton (Gossypium spp.)

Cotton (Gossypium spp.) is the fifth largest oil crop in the world, and cottonseed provides abundant vegetable oil resources and industrial bioenergy fuels for people; therefore, it is of practical significance to increase the oil content of cotton seeds for improving the oil yield and economic benefits of planting cotton. Long-chain acyl-coenzyme A (CoA) synthetase (LACS) capable of catalyzing the formation of acyl-CoAs from free fatty acids has been proven to significantly participate in lipid metabolism, of which whole-genome identification and functional characterization of the gene family have not yet been comprehensively analyzed in cotton. In this study, a total of sixty-five LACS genes were confirmed in two diploid and two tetraploid Gossypium species, which were divided into six subgroups based on phylogenetic relationships with twenty-one other plants. An analysis of protein motif and genomic organizations displayed structural and functional conservation within the same group but diverged among the different group. Gene duplication relationship analysis illustrates the LACS gene family in large scale expansion through WGDs/segmental duplications. The overall Ka/Ks ratio indicated the intense purifying selection of LACS genes in four cotton species during evolution. The LACS genes promoter elements contain numerous light response cis-elements associated with fatty acids synthesis and catabolism. In addition, the expression of almost all GhLACS genes in high seed oil were higher compared to those in low seed oil. We proposed LACS gene models and shed light on their functional roles in lipid metabolism, demonstrating their engineering potential for modulating TAG synthesis in cotton, and the genetic engineering of cottonseed oil provides a theoretical basis.

Optimizing Urban Stock Space through District Boundary Reorganization: Hangzhou’s Administrative Adjustment

Since China is the largest developing country in the world, its urban reconstruction has significance for countries undergoing rapid urbanization. The recent development of its central cities has gradually changed from large-scale incremental expansion to stock optimization. District boundary reorganization has become a new trend in reforming China’s administrative division of central cities. From the perspective of scalar reorganization and regional reconstruction, the adjustment of urban administrative divisions can be regarded as a regional construction strategy initiated by city governments to reshape the spatial structure, improve the governance relationship, and enhance the cities’ competitiveness. This study takes Hangzhou, an important central city in eastern China, as a case study to illustrate two ways in which a central city can optimize its urban spaces through district boundary recombination: scale recombination and regional recombination. The findings demonstrate two advantages of district boundary reorganization for China’s central cities: it eases the integration of new city districts and urban areas and promotes balanced development within the city. In the future, more Chinese cities will likely choose to reorganize their district boundaries.

Validation of an automated viable cell counting assay for GMP manufacturing of human induced pluripotent stem cells

Human induced pluripotent stem cells (hiPSCs) and their derivatives are currently manufactured as advanced therapy medicinal products (ATMPs) for clinical use in tissue replacement applications. To achieve this aim, hiPSC production must be performed in compliance with good manufacturing practices (GMP). Specifically, analytical methods used for process monitoring must be validated for their intended purposes. A pivotal analysis for large-scale cell manufacturing is fast, reliable and it requires consistent viability assessment. Cell viability is a crucial quality attribute to guarantee product reproducibility and robust manufacturing process. Hence, we validated the use of an image cytometer–based method for the determination of hiPSC viability, focusing on its specificity, linearity, range, accuracy, and intra- and inter-operator reproducibility. To gain additional information, we also assessed the stability of hiPSC viability. This validation of an analytical procedure for hiPSCs demonstrates the feasibility of translation from research protocols to manufacturing processes via the application of more efficient automated methods for viability measurement in comparison with conventional flow cytometry. In conclusion, the present work paves the way for the application of this method as a quality control test during large-scale GMP hiPSC expansion.

Preparation and performance study of scalable high-efficiency solar steam generated system

Solar-driven evaporation is gaining more and more attention due to its widespread use, including desalination, power generation and solar fuel production. Among them, desalination is the most needed because water is the source of life. In this work, we have developed a new efficient and low-cost solar desalination system consisting of non-woven fabrics, carbon powder and polystyrene foam. We designed the experiments under a solar irradiation of 1000 W m−2, an ambient temperature of 25 °C and a humidity of 0.4, controlled in a laboratory performance. In this system, the solar steam conversion efficiency is greatly improved due to the fibrous structure and high absorbance of the carbonized non-woven fabric. The practical application of the system was also tested. The experimental results show that the solar steam conversion efficiency of the new system can reach 85.3% under the solar radiation intensity of 1000 W m−2. The corresponding evaporation rate is 1.23 kg (m2 h)−1. In addition, the effects of wind speed, solar irradiance, ambient temperature, and material absorptivity on evaporation efficiency are simulated. The thermal environment parameters for enhancing evaporation efficiency are given. The performance of the solar interface evaporation system is compared in terms of the first law of thermodynamics and external energy. Finally, the economics and the analysis of the device are performed, the cost of interface evaporation material is $1.05 m−2. Due to the low cost of raw materials, simple material preparation and high evaporation efficiency, the system provides the possibility for the large-scale expansion and application of solar interface evaporation.

Abstract 1775: Expansion of human T cells using xenogeneic free and gamma irradiated human platelet lysate

Abstract Commercially available human platelet lysate (hPL) is produced using expired human platelets obtained from accredited blood banks in the United States. These platelets were originally intended for use in patient transfusion. The safety of platelets used in transfusion is managed by the U.S. Food & Drug Administration (FDA), as well as the American Association of Blood Banks (AABB). These organizations set standards, including testing for transmissible diseases. The United States record for blood safety is well established, with extremely low rates of disease transmission, making the platelet units used for hPL manufacture low risk. The Covid-19 pandemic has increased awareness of emerging infectious diseases, even though transmission of Covid-19 via blood transfusion has not been documented. For that reason, gamma irradiated hPL offers an additional safety measure in the clinic. Chimeric Antigen Receptor (CAR) expressing T-cells have demonstrated potent clinical efficacy in patients with hematological malignancies. In addition, there are several phase I clinical trials evaluating the use of CAR-T-cells for targeting of solid tumor-associated antigens. Some of the challenging issues found during production of CAR-T cells are the efficiency of T cell transduction to generate CAR-T cells, the expansion of T cells to clinically relevant numbers and the long-term survival in vivo of the therapeutic cells. The use of human platelet lysate has been demonstrated to improve these issues. Our data from experiments performed using human CD3+ from donors demonstrates that human platelet lysates offer an improved performance on T cell expansion versus serum derived products. hPL efficiently promotes T cell expansion, with higher cell yields and lower cell exhaustion rate. Additionally, we efficiently developed a protocol for suspension culture of T cells, which could facilitate the large-scale expansion of allogeneic CAR-T cells. Citation Format: Vanesa Alonso-Camino. Expansion of human T cells using xenogeneic free and gamma irradiated human platelet lysate [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 1775.

Cloud Storage Systems with Secure Attribute-Based Access Control

This research presents a novel approach to safe access procedure in cloud storage structure by addressing the challenges associated with managing and distributing complex secret keys. The proposed procedure utilizes attribute-based access control and incorporates a client process, an authentication terminal process, and a storage terminal process. The client generates a main secret key and public parameters for each user using an attribute password mechanism. The authentication terminal maintains user attribute, file attribute, and attribute secret key databases. Access control is determined by constructing an access control string from the attributes of the user, file, and file operation type, enabling fine-grained access control and accommodating large-scale user dynamic expansion. Consequently, the access control costs of the cloud storage structure are significantly reduced, while offering a flexible, effective, and secure access control mechanism for safe storage structure access.

Volumetric imaging of human mesenchymal stem cells (hMSCs) for non-destructive quantification of 3D cell culture growth.

The adoption of cell-based therapies into the clinic will require tremendous large-scale expansion to satisfy future demand, and bioreactor-microcarrier cultures are best suited to meet this challenge. The use of spherical microcarriers, however, precludes in-process visualization and monitoring of cell number, morphology, and culture health. The development of novel expansion methods also motivates the advancement of analytical methods used to characterize these microcarrier cultures. A robust optical imaging and image-analysis assay to non-destructively quantify cell number and cell volume was developed. This method preserves 3D cell morphology and does not require membrane lysing, cellular detachment, or exogenous labeling. Complex cellular networks formed in microcarrier aggregates were imaged and analyzed in toto. Direct cell enumeration of large aggregates was performed in toto for the first time. This assay was successfully applied to monitor cellular growth of mesenchymal stem cells attached to spherical hydrogel microcarriers over time. Elastic scattering and fluorescence lightsheet microscopy were used to quantify cell volume and cell number at varying spatial scales. The presented study motivates the development of on-line optical imaging and image analysis systems for robust, automated, and non-destructive monitoring of bioreactor-microcarrier cell cultures.

Genetic footprints of a rapid and large-scale range expansion: the case of cyclic common vole in Spain.

In the Anthropocene, many species are rapidly shifting their ranges in response to human-driven habitat modifications. Studying patterns and genetic signatures of range shifts helps to understand how species cope with environmental disturbances and predict future shifts in the face of global environmental change. We investigated the genetic signature of a contemporary wide-range expansion observed in the Iberian common vole Microtus arvalis asturianus shortly after a colonization event. We used mtDNA and microsatellite data to investigate patterns of genetic diversity, structure, demography, and gene flow across 57 localities covering the historical range of the species and the newly colonized area. The results showed a genetic footprint more compatible with a true range expansion (i.e. the colonization of previously unoccupied areas), than with a model of "colonization from within" (i.e. local expansions from small, unnoticed populations). Genetic diversity measures indicated that the source population was likely located at the NE of the historical range, with a declining gradient of genetic diversity towards the more recently invaded areas. At the expansion front, we observed the greatest gene flow and smallest pairwise differences between nearby localities. Both natural landscape features (rivers) and recent anthropogenic barriers (roads, railways) explained a large proportion of genetic variance among populations and had a significant impact on the colonization pathways used by voles.

Hologenome analysis reveals independent evolution to chemosymbiosis by deep-sea bivalves

BackgroundBivalves have independently evolved a variety of symbiotic relationships with chemosynthetic bacteria. These relationships range from endo- to extracellular interactions, making them ideal for studies on symbiosis-related evolution. It is still unclear whether there are universal patterns to symbiosis across bivalves. Here, we investigate the hologenome of an extracellular symbiotic thyasirid clam that represents the early stages of symbiosis evolution.ResultsWe present a hologenome of Conchocele bisecta (Bivalvia: Thyasiridae) collected from deep-sea hydrothermal vents with extracellular symbionts, along with related ultrastructural evidence and expression data. Based on ultrastructural and sequencing evidence, only one dominant Thioglobaceae bacteria was densely aggregated in the large bacterial chambers of C. bisecta, and the bacterial genome shows nutritional complementarity and immune interactions with the host. Overall, gene family expansions may contribute to the symbiosis-related phenotypic variations in different bivalves. For instance, convergent expansions of gaseous substrate transport families in the endosymbiotic bivalves are absent in C. bisecta. Compared to endosymbiotic relatives, the thyasirid genome exhibits large-scale expansion in phagocytosis, which may facilitate symbiont digestion and account for extracellular symbiotic phenotypes. We also reveal that distinct immune system evolution, including expansion in lipopolysaccharide scavenging and contraction of IAP (inhibitor of apoptosis protein), may contribute to the different manners of bacterial virulence resistance in C. bisecta.ConclusionsThus, bivalves employ different pathways to adapt to the long-term co-existence with their bacterial symbionts, further highlighting the contribution of stochastic evolution to the independent gain of a symbiotic lifestyle in the lineage.

A Multi-Stage Bioprocess for the Expansion of Rodent Skin-Derived Schwann Cells in Computer-Controlled Bioreactors

Regenerative therapies for the treatment of peripheral nerve and spinal cord injuries can require hundreds of millions of autologous cells. Current treatments involve the harvest of Schwann cells (SCs) from nerves; however, this is an invasive procedure. Therefore, a promising alternative is using skin-derived Schwann cells (Sk-SCs), in which between 3-5 million cells can be harvested from a standard skin biopsy. However, traditional static planar culture is still inefficient at expanding cells to clinically relevant numbers. As a result, bioreactors can be used to develop reproducible bioprocesses for the large-scale expansion of therapeutic cells. Here, we present a proof-of-concept SC manufacturing bioprocess using rat Sk-SCs. With this integrated process, we were able to simulate a feasible bioprocess, taking into consideration the harvest and shipment of cells to a production facility, the generation of the final cell product, and the cryopreservation and shipment of cells back to the clinic and patient. This process started with 3 million cells and inoculated and expanded them to over 200 million cells in 6 days. Following the harvest and post-harvest cryopreservation and thaw, we were able to maintain 150 million viable cells that exhibited a characteristic Schwann cell phenotype throughout each step of the process. This process led to a 50-fold expansion, producing a clinically relevant number of cells in a 500 mL bioreactor in just 1 week, which is a dramatic improvement over current methods of expansion.