Large Pressure Research Articles

Comparing statistical forecasting methods for modelling respiratory virus healthcare demand

Abstract Background Emergency departments in the United Kingdom are under sustained pressure with rising demand for healthcare, gradually decreasing the functionality of the NHS. Forecasts can aid management in the allocation of appropriate healthcare supplies and staffing needs in advance of an expected surge, as well as allow stakeholders to assess most likely outcomes and potential worst-case scenarios. This is especially valuable during the seasonal waves of respiratory infections. Respiratory viruses are usually concentrated in a 6-8 week seasonal surge during the winter period, creating large pressure on the health system. Current forecast models’ performance depreciates significantly after 1 week. This analysis will forecast 6 weeks of respiratory health admissions, comparing traditional and newly emerging forecasting methods. Methods Using 20 years of English hospital admissions data, we evaluated both within season training models (2-20 weeks) and longer term seasonal models (1-10 years), to forecast 6 weeks of hospital admissions. Respiratory admissions were defined according to ARI/ILI ICD10 codes assigned in hospital, stratified by age and region. Models were iteratively constructed, trained and probabilistic forecasts were evaluated across the respiratory season of September to April using Weighted Interval Score. Averages of each model across iterations were generated for comparison. Results For non-seasonal models, shorter model training lengths created better forecast models for 6 weeks. For seasonal models, optimal training length varied across model complexity. Simple models performed best at forecasting 1 week, however performance deteriorated the most as the forecast window increased. Overall, the Prophet model performed best on average at forecasting 6 weeks across all scenarios. Conclusions Applying our framework for optimal model selection in forecasting analysis will help to inform better practice for future health demand modelling. Key messages • This analysis will inform better practice for future health demand forecasting 6 weeks in advance, allowing for preparation of resources for seasonal surges in healthcare demand. • This analysis suggests using models with modest levels of increased complexity over simpler models could significantly improve forecast performance in the short-medium term.

Design, Evaluation & Implementation of a Novel MRI-compatible Physiologic Loading Simulator for Ex-Vivo Joints.

Quantitative magnetic resonance imaging (qMRI), in combination with mechanical testing, offers potential to investigate how loading is related to joint and tissue function. However, current testing devices compatible with MRI are often limited to uniaxial compression, often applying low loads, or loading individual tissues (instead of multiple), while more complex simulators do not facilitate MRI. Hence, in this work, we designed, built and tested (N=1) an MRI-compatible multiaxial load-control system which enables scanning cadaveric joints (healthy or pathologic) loaded to physiologically-relevant levels. Testing involved estimating and validating physiologic loading conditions before implementing them experimentally on cadaver knees to simulate and image gait loading (stance and swing). The resulting design consisted of a portable loading device featuring pneumatic actuators to reach a combined loading scenario, including axial compression (=2.5 kN), shear (=1 kN), bending (=30 N·m) and muscle tension. Initial laboratory testing was carried out; specifically, the device was instrumented with force and pressure sensors to evaluate loading and contact response repeatability in one cadaver knee specimen. This loading system was able to simulate healthy or pathologic gait with reasonable repeatability (e.g., 1.23 to 2.91 % coefficient of variation for axial compression), comparable to current state-of-the-art simulators, leading to generally consistent contact responses. Contact measurements demonstrated a tibiofemoral to patellofemoral load transfer with knee flexion and large contact pressures concentrated over small sites between the femoral cartilage and menisci, agreeing with experimental studies and numerical simulations in the literature.

Loudness reduction of household refrigerator by structural modification

In this study, loudness reduction of a household refrigerator was attempted by the structure modification. At first, loudness of the radiated noise was analyzed at various operational condition and the loudness was observed to be increased at a specific compressor rotational speed and the large sound pressure level (SPL) at 100 Hz was the main factor. Through the vibration analysis of the refrigerator body, the SPL was found to be increased largely by the resonance between the compressor rotational order input force and the natural frequency of the refrigerator body. Then, for the reduction of the sound, the rubber bush hardness of the compressor mount was softened and the loudness was decreased. However, the door vibration at low rotational speed was increased. Accordingly, we attempted to modify the body structure for the loudness reduction to compatible with small door vibration. Vibration mode analysis indicated that the high contributing vibration behavior of the body increasing the loudness and a countermeasure was applied to constrain the behavior. Finally, the loudness was decreased about 30% and the vibration was not increased at the low rotational speed by the countermeasure.

A brain-wide solute transport model of the glymphatic system.

Brain waste is largely cleared via diffusion and advection in cerebrospinal fluid (CSF). CSF flows through a pathway referred to as the glymphatic system, which is also being targeted for delivering drugs to the brain. Despite the importance of solute transport, no brain-wide models for predicting clearance and delivery through perivascular pathways and adjacent parenchyma existed. We devised such a model by upgrading an existing model of CSF flow in the mouse brain to additionally solve advection-diffusion equations, thereby estimating solute transport. We simulated steady-state transport of 3 kDa dextran injected proximal to the perivascular space (PVS) of the middle cerebral artery, mimicking in vivo experiments. We performed a sensitivity analysis of 11 biological properties of PVSs and brain parenchyma by repeatedly simulating solute transport with varying parameter values. Parameter combinations that led to a large total pressure gradient, poor CSF perfusion or a steep solute gradient were deemed unrealistic. Solute concentrations in parenchyma were most sensitive to changes in pial PVS size, as this parameter linearly affects volume flow rates. We also found that realistic transport requires both highly permeable penetrating PVSs and high-resistance parenchyma. This study highlights the potential of brain-wide models to provide insights into solute transport processes.

Development of Johnson-Cook-Distinct Lattice Spring Model and its application in projectile penetration into metal targets

Projectile penetration into metal targets plays an important role in modern protective structure engineering, damage assessment in terrorist attacks, and car clash accident analysis, etc. The penetration process of metal target has been characterized by large deformation, high temperature, high pressure, and dynamic damage, which can be classified as a continuous-discontinuous dynamical process. To capture the dynamic responses of metal materials subjected to high-speed penetration, Johnson-Cook model has been implemented in a continuum-discrete model, namely, Distinct Lattice Spring Model (DLSM). This is achieved by redefining the constitutive model in DLSM, with plasticity hardening (plastic strain effects), strain rate, temperature, damage evolution, equation of state, etc., being taken into account, thus developing a new numerical model called JC-DLSM model. This model is validated through studying Taylor rod high-speed impact experiments, thin metal plate penetration experiments, and projectile impact experiments on titanium alloy targets. Good agreement between numerical modelling and experimental data has been achieved for all cases considered, thereby demonstrating the capability of JC-DLSM to reproduce the damage characteristics and ballistic limits of metals under high-speed impact/penetration. Then, the impacts of projectile shape, metal target surface configuration on the characteristics of penetration damage patterns have been investigated. This contributes to a better understanding of the damage mechanisms of metal targets upon penetration or impact, facilitating the computational means for analysing and optimizing the penetration resistance of protective engineering structures.

Simple fabrication of breathable poly (vinylidene fluoride) fibrous membranes decorated by silica nanoparticles with enhanced waterproof property

In recent years, scientists have undertaken various preparation approaches to improve the waterproof and moisture permeability of fiber membranes, among which electrospinning has emerged as the simplest and most effective one. In this paper, poly (vinylidene fluoride) /silica (PVDF/SiO2) fibrous membranes were prepared by one-step electrospinning combined with heat treatment. The morphology, surface wettability, water resistance and moisture permeability of the fibrous membranes were also characterized. When the concentration of PVDF was 12 wt.%, the nanofiber membranes exhibited the most uniform and optimal morphology. In addition, the fiber membranes not only exhibited a high water contact angle (WCA) (135.56 °) and a small maximum pore size (dmax) (1.77 μm), but also demonstrated a large hydrostatic pressure (116.86 kPa) and a moderate water vapor transmission rate (WVTR) (3.53 kg m-2 d-1). The PVDF/SiO2 fibrous membranes with good waterproof and moisture permeability obtained in this work are expected to improve the comfort of protective textiles and expand the application range.

AXES-2MRS: A new all-sky catalogue of extended X-ray galaxy groups

Context. Understanding baryonic physics at the galaxy-group level is a prerequisite for cosmological studies of the large-scale structure. One poorly understood aspect of galaxy groups is related to the properties of their hot intragroup medium. The well-studied X-ray groups have strong cool cores by which they were selected, so expanding the selection of groups is currently an important avenue in uncovering the diversity within the galaxy group population. Aims. We present a new all-sky catalogue of X-ray-detected groups (AXES-2MRS) based on the identification of large X-ray sources found in the ROSAT All-Sky Survey (RASS) with the Two Micron Redshift Survey (2MRS) Bayesian Group Catalogue. We studied the basic properties of these galaxy groups to gain insights into the effect of different group selections on the properties. Methods. In addition to X-ray luminosity from shallow survey data of RASS, we obtained detailed X-ray properties of the groups by matching the AXES-2MRS catalogue to archival X-ray observations by XMM-Newton and complemented this by adding the published XMM-Newton results on galaxy clusters in our catalogue. We analysed temperature and density to the lowest overdensity accessible by the data, obtaining hydrostatic mass estimates at a uniform overdensity of 10 000 times the critical, M10 000, and comparing them to the velocity dispersions of the groups. We explored the relationship between X-ray and optical properties of AXES-2MRS groups through the σv−LX, σv−kT, kT−LX, σv−M, and c200−LX scaling relations. Results. We find a large spread in the central mass ( M10 000), measured by XMM-Newton, to virial mass (M200), inferred by the velocity dispersion, ratios for galaxy groups. This can either indicate that large non-thermal pressure of galaxy groups affects our X-ray mass measurements or the effect of a diversity of halo concentrations on the X-ray properties of galaxy groups. Previous catalogues based on detecting the peak of the X-ray emission preferentially sample the high-concentration groups. In contrast, our new catalogue uncovered many low-concentration groups, completely revising our understanding of X-ray groups.

Compressible ocean waves generated by sudden seabed rise near a step-type topography

A typical tsunami generation occurs through submarine earthquakes leading to large volume displacement. The corresponding mathematical problem involves modeling surface water waves generated by an arbitrary temporal motion of the ocean floor. The propagation of tsunami wave and the subsequent scattering from a sudden drop in bathymetry away from the ground motion is studied following linearized water wave theory and a weakly compressible ocean, including static oceanic background compression. The Fourier transformation and eigenfunction expansion techniques are employed to find the surface displacement and pressure profiles by leveraging appropriate matching conditions between regions of different depths. A novel energy balance relationship is derived by considering both the pure-gravity and acoustic-gravity modes. The model is validated in the limit that the depth difference approaches zero, showing a vanishing reflection contribution from the depth change. An efficient numerical code is developed that accurately captures the contribution of the cutoff frequencies of acoustic-gravity modes. Apart from the time-domain propagation of tsunami waves away from the origin, standing wave formations are observed within the shallow region, supported by significantly large pressure fluctuations in time. These standing waves or, equivalently, the pressure fluctuations sustain longer for larger ocean depth. The increase in tsunami speed in the deeper region is readily visible in the time-domain simulations. A three-dimensional axisymmetric solution is also developed, and results show a more gradual sloping tsunami wavefront compared to the equivalent two-dimensional solution for shallower depths. Animation movies corresponding to the two- and three-dimensional surface profiles are provided for better visualization.

Evolving Outer Heliosphere: Tracking Solar Wind Transients from 1 au to the VLISM with IBEX and Voyager 1

Interstellar Boundary Explorer (IBEX) observations of energetic neutral atom (ENA) fluxes from the heliosphere have greatly enriched our understanding of the interaction of the solar wind (SW) with the local interstellar medium (LISM). However, there has been recent controversy surrounding the inability of most ENA models to produce as high an intensity of ∼0.5–6 keV ENAs as IBEX observes at 1 au, especially as a function of time. In our previous study (E. J. Zirnstein et al.), we introduced a new model that utilizes a data-driven magnetohydrodynamic simulation of the SW–LISM interaction to propagate pickup ions through the heliosheath (HS) after they are nonadiabatically heated at the heliospheric termination shock. E. J. Zirnstein et al. only simulated and analyzed IBEX observations from the direction of Voyager 2. In this study, we expand our model to include fluxes from the direction of Voyager 1, as well as in the low-latitude part (middle) of the ribbon (10° below the ecliptic plane). We show that the model results at Voyager 1 are consistent with E. J. Zirnstein et al.’s results at Voyager 2 in terms of a secondary ENA source contribution of ≲20% from both directions. Our results in the middle of the ribbon also reproduce the data, when including a time-dependent secondary ENA source. Finally, we demonstrate with our simulation that three large pressure waves likely merged in the VLISM and were observed by Voyager 1 as “pf2,” while at least one of the wave’s effects in the HS was observed by IBEX as a brief enhancement in ENA flux in early 2016.

Experimental study on current density distribution characteristics in a novel oxygen recirculation system of dead-ended proton exchange membrane fuel cell

ABSTRACT Dead-ended proton exchange membrane fuel cells (PEMFCs) using pure hydrogen and oxygen can improve fuel efficiency and cell performance, making them widely applicable in enclosed spaces. However, dead-ended PEMFCs are prone to flooding, which reduces cell performance and service life. To address this issue, an oxygen recirculation system utilizing oscillating flow generated by pressure differences is designed in this study. This system not only achieves close to 100% fuel utilization but also optimizes water management and enhances current density uniformity. The optimal oxygen cycling conditions were selected based on current density uniformity and cell performance enhancement. The results show that a larger pressure difference amplitude improves water removal from the cell; however, excessively large pressure differences can lead to unstable cell operation. The oscillating flow generated by pressure differences significantly improved current density uniformity, particularly at higher output load currents. Current density uniformity improved by 16.29% at a current of 75 A. The experiment demonstrates that the best current density uniformity is achieved when managing water using a pressure difference at 4-minute intervals, as longer or shorter oscillation intervals are unsuitable for dead-ended performance.

Red blood cell passage through deformable interendothelial slits in the spleen: Insights into splenic filtration and hemodynamics

The spleen constantly clears altered red blood cells (RBCs) from the circulation, tuning the balance between RBC formation (erythropoiesis) and removal. The retention and elimination of RBCs occur predominantly in the open circulation of the spleen, where RBCs must cross submicron-wide inter-endothelial slits (IES). Several experimental and computational studies have illustrated the role of IES in filtrating the biomechanically and morphologically altered RBCs based on a rigid wall assumption. However, these studies also reported that when the size of IES is close to the lower end of clinically observed sizes (less than 0.5 μm), an unphysiologically large pressure difference across the IES is required to drive the passage of normal RBCs, sparking debates on the feasibility of the rigid wall assumption. In this work, We propose two deformable IES models, namely the passive model and the active model, aiming to explore the impact of the deformability of IES on the filtration function of the spleen. In the passive model, we implement the worm-like string model to depict the IES’s deformation as it interacts with blood plasma and allows RBC to traverse. In contrast, the active model involved regulating the IES deformation based on the local pressure surrounding the slit. To demonstrate the validity of the deformable model, we simulate the filtration of RBCs with varied size and stiffness by IES under three scenarios: (1) a single RBC traversing a single slit; (2) a suspension of RBCs traversing an array of slits, mimicking in vitro spleen-on-a-chip experiments; (3) RBC suspension passing through the 3D spleen filtration unit known as’the splenon’. Our simulation results of RBC passing through a single slit show that the deformable IES model offers more accurate predictions of the critical cell surface area to volume ratio that dictate the removal of aged RBCs from circulation compared to prior rigid-wall models. Our biophysical models of the spleen-on-a-chip indicate a hierarchy of filtration function stringency: rigid model > passive model > active model, providing a possible explanation of the filtration function of IES. We also illustrate that the biophysical model of ‘the splenon’ enables us to replicate the ex vivo experiments involving spleen filtration of malaria-infected RBCs. Taken together, our simulation findings indicate that the deformable IES model could serve as a mesoscopic representation of spleen filtration function closer to physiological reality, addressing questions beyond the scope of current experimental and computational models and enhancing our understanding of the fundamental flow dynamics and mechanical clearance processes within in the human spleen.

Exotic Stable Branches with Efficient TOV Sequences

Modern inference schemes for the neutron star (NS) equation of state (EoS) require large numbers of stellar models constructed with different EoS, and these stellar models must capture all the behavior of stable stars. I introduce termination conditions for sequences of stellar models for cold nonrotating NSs that can identify all stable stellar configurations up to arbitrarily large central pressures along with an efficient algorithm to build accurate interpolators for macroscopic properties. I explore the behavior of stars with both high- and low-central pressures. Interestingly, I find that EoSs with monotonically increasing sound-speed can produce multiple stable branches (twin stars) and that large phase transitions at high densities can produce stable branches at nearly any mass scale, including sub-solar masses while still supporting stars with M > 2 M ⊙. I conclude with some speculation about the astrophysical implications of this behavior.

Effect of macro- and micro-morphology on fluid film properties based on plasto-elastohydrodynamic lubrication

Purpose The developed plasto-elastohydrodynamic lubrication (PEHL) model is used to demonstrate the permanent change of macro morphology by critical high local stress at micro asperities in contact, which may further affect the fluid-film characteristics. Design/methodology/approach Geometric morphology is integrated into the PEHL model to elucidate the fluid-film properties governed by both macro- and micromorphologies. Findings Results show the model, accounting for combination of elastic and plastic deformations, realistically reveals fluid film distribution affected by the significant pressure highly concentrated within surface micro roughness interaction. The designed macroscopic textured surface mitigates the fluid film rupture phenomenon and prevents accumulated wear degradation from plastic deformation. Originality/value The PEHL model takes into account both elastic and plastic deformations and realistically reveals the fluid film distribution affected by large pressures that are highly concentrated in surface micro-roughness interactions. The macro-textured surfaces are designed to mitigate fluid film rupture phenomena and prevent cumulative wear caused by plastic deformation. Peer review The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-05-2024-0170/

Biomimetic Contact Behavior Inspired Tactile Sensing Array with Programmable Microdomes Pattern by Scalable and Consistent Fabrication.

Flexible sensor arrays have attracted extensive attention in human-computer interaction. However, realizing high-performance sensor units with programmable properties, and expanding them to multi-pixel flexible arrays to maintain high sensing consistency is still struggling. Inspired by the contact behavior of octopus antenna, this paper proposes a programmable multistage dome structure-based flexible sensing array with robust sensing stability and high array consistency. The biomimetic multistage dome structure is pressurized to gradually contact the electrode to achieve high sensitivity and a large pressure range. By adjusting the arrangement of the multistage dome structure, the pressure range and sensitivity can be customized. More importantly, this biomimetic structure can be expanded to a multi-pixel sensor array at the wafer level with high consistency through scalable and high-precision imprinting technologies. In the imprinting process, the conductive layer is conformally embedded into the multistage dome structure to improve the stability (maintain stability over 22000 cycles). In addition, the braced isolation structure is designed to effectively improve the anti-crosstalk performance of the sensor array (crosstalk coefficient: 26.62dB). Benefitting from the programmable structural design and high-precision manufacturing process, the sensor array can be customized and is demonstrated to detect human musculation in medical rehabilitation applications.

Universal Diffusion in Coulomb Crystals.

Diffusion coefficients for crystallized Coulomb plasmas are essential microphysics input for modeling white dwarf cores and neutron star crusts but are poorly understood. In this work we present a model for diffusion in Coulomb crystals. We show that melting and diffusion follow the same universal scaling such that diffusion is independent of screening. Our simulations show, contrary to prevailing wisdom, that the formation of vacancies is not suppressed by the large pressure. Rather, vacancy formation and hole diffusion is the dominant mode of self-diffusion in Coulomb crystals.

Research on the technical improvement of the turbine runner of a power station based on improving stability

AbstractIn view of problems such as the narrow efficiency area, large hydraulic vibration area, pressure pulsation, and serious sediment wear of turbines at the Futang hydropower station, the technical transformation of turbine runners was carried out by modifying the blade shape and increasing the blade thickness, and a combination of numerical simulations based on shear stress transport k–ω turbulence model and tests was adopted to improve the operational stability of power station units. Calculation and testing demonstrate an enlargement of the high‐efficiency zone. Specifically, the optimal efficiency of the runner increases by 0.37%, while the rated efficiency rises by 0.19%. Significant reductions are observed in pressure pulsation within the draft tube and vaneless area decrease of approximately 50%. There is a high‐frequency pressure pulsation in the vaneless zone and the runner under low‐load conditions, and the influence of dynamic and static interference gradually weakens with the increase of opening. The draft tube is prone to eccentric vortex bands under partial working conditions, which causes the unit to be affected by low‐frequency pulsation. This optimization also leads to a notable decrease in runner blade wear, with the maximum sand and water velocity reduced from 45 to 40 m/s, resulting in a 30% reduction in sand wear. Moreover, there is a substantial enhancement in the runner's stiffness, with the thickness of the blade near the high stress area of the upper crown and lower ring increasing by over 50%, and the weight of each individual blade increasing by more than 50%. These research findings validate that modifying the runner blade effectively improves flow patterns, reduces eddy current generation, minimizes pressure pulsation, widens the high‐efficiency zone, decreases wear, and enhances the operational stability of the unit. The technical transformation method and research results of this study have important guiding significance for similar technical transformation of other power stations

A novel membrane-based absorber for sorption heat transformers: A comparative experimental study of a thin-film absorbent and salt-impregnated adsorbents

The present study proposes a novel membrane-based sorber bed with stationary solution film for oscillatory sorption heat transformers. A custom-built gravimetric large pressure jump setup is used to experimentally compare the performance of the novel membrane-based sorber bed with a Lithium Bromide/Silica gel solid composite sorbent synthesized using the salt-impregnation method. It is shown that the current membrane-based sorber bed provides up to about four times the specific cooling power and more than twice the cooling power density of solid sorbents, demonstrating the capability of the present sorber bed as a promising alternative to solid sorbents. The specific cooling power, cooling power density, and energy storage density of about 2.8 kW/kg, 470 kW/m3, and 269 MJ/m3 are experimentally obtained, respectively. According to experimental results, it is observed that the film thickness plays a significant role in sorption dynamics, while the effect of membrane mass transfer resistance on sorption dynamics is less than 10%. In addition, the present membrane-based sorber bed is analytically modeled, and the results are validated with present experimental data. The present analytical model is also used to investigate the effects of design parameters on the performance metrics.

Natural gas hydrate dissolution kinetics in sandy sediments: Implications for massive water production in field tests

The tremendous natural gas hydrate (NGH) reserve in globe makes it a promising future energy. The field test production indicates that massive water production may occur in the depressurization of sandy NGH reservoirs. The dissolution of NGH may play a significant role in this process. Therefore, quantifying the NGH dissolution kinetics and clarifying the relations between NGH dissolution and water production is crucial. In this work, a modified excess water method was developed to prepare quasi-homogenous water-saturated methane hydrate (MH) bearing sediments (HBS). The MH dissolution kinetics were investigated by flowing methane-free water through the HBS. The MH dissolution process could be divided into the rapid stage and the decayed stage. Preferential flow channels may form by the inhomogeneous MH dissolution. Water flux has a significant impact on the MH dissolution kinetics via affecting the mass transfer and the hydrate-water exposure in flow channels. When increasing the water fluxes from 2.0 mL·cm−2·min−1 to 6.1 mL·cm−2·min−1, the MH dissolution rate initially ramped up from 15.4 cm·d−1 to 36.0 cm·d−1 (mass transfer dominates), and then decreased to 28.7 cm·d−1 (flow channels dominate). MH saturation has an influence on dissolution kinetics. The MH dissolution rate increased from 27.1 cm·d−1 to 44.2 cm·d−1 when the MH saturations increased from 11.8 % to 60.1 %. Experimental results indicate that the preferential flow channels by inhomogeneous NGH dissolution could contribute to the massive water production in the depressurization of NGH reservoir. Large pressure gradient in NGH production may accelerate the NGH dissolution and the following massive water production. Homogeneous high-saturation HBS could more facilitate the hydrate-water exposure for dissolution, which could retard the onset of massive water production. This work may ignite some new thoughts on the water control and production optimization strategies in sandy NGH exploitation.

Breaking the Saturation of Sensitivity for Ultrawide Range Flexible Pressure Sensors by Soft-Strain Effect.

The flexible pressure sensors with a broad pressure range and unsaturated sensitivity are highly desired in practical applications. However, pressure sensors by piezoresistive effect are always limited by the compressibility of sensing layers, resulting in a theoretically decreasing sensitivity of less than 100%. Here, a unique strategy is proposed that utilizes the strain effect, simultaneously achieving a trade-off between a wider pressure detection range and unsaturated sensitivity. Ascribed to the strain effect of sensing layers induced by interlaced microdomes, the sensors possess an increased sensitivity (5.22-70 MPa-1) over an ultrawide pressure range (45 Pa-4.1 MPa), a high-pressure resolution (5 Pa), fast response/recovery time (30/45 ms), and a robust response under a high-pressure loading of 3.5 MPa for more than 5000 cycles. These superior sensing performances allow the sensor to monitor large pressure. The flexible pressure sensor array can assist doctors in restoring the neutral mechanical axis, tracking knee flexion angles, and extracting gait features. Moreover, the flexible sensing array can be integrated into the joint motion surveillance system to map the balance medial-lateral contact forces on the metal compartments in real time, demonstrating the potential for further development into precise medical human-machine interfaces during total knee replacement surgery.

Cell spheroid viscoelasticity is deformation-dependent

Tissue surface tension influences cell sorting and tissue fusion. Earlier mechanical studies suggest that multicellular spheroids actively reinforce their surface tension with applied force. Here we study this open question through high-throughput microfluidic micropipette aspiration measurements on cell spheroids to identify the role of force duration and spheroid deformability. In particular, we aspirate spheroid protrusions of mice fibroblast NIH3T3 and human embryonic HEK293T homogeneous cell spheroids into micron-sized capillaries for different pressures and monitor their viscoelastic creep behavior. We find that larger spheroid deformations lead to faster cellular retraction once the pressure is released, regardless of the applied force. Additionally, less deformable NIH3T3 cell spheroids with an increased expression level of alpha-smooth muscle actin, a cytoskeletal protein upregulating cellular contractility, also demonstrate slower cellular retraction after pressure release for smaller spheroid deformations. Moreover, HEK293T cell spheroids only display cellular retraction at larger pressures with larger spheroid deformations, despite an additional increase in viscosity at these larger pressures. These new insights demonstrate that spheroid viscoelasticity is deformation-dependent and challenge whether surface tension truly reinforces at larger aspiration pressures.