Abstract Water samples were collected from 33 of the 54 heating, ventilation, and air conditioning (HVAC) humidification system water reservoir pans in a large office building. Two different forms of humidification are used in this building: air washers and steam injection. Thirty-eight samples were cultured for human-source and heterotrophic bacteria, thermophilic organisms, and fungi. While one microbe, an unidentified bacterium, was found on more plates than any other organism, Streptococcus and Pseudomonas were found to be the most predominant microorganisms. In a comparison of the air wash and steam injection humidification systems, only small quantities of bacteria were found in the steam systems, while water collected from the air wash systems was highly contaminated. Data were also available to test the effectiveness of the cleaning system. A sample collected one day before cleaning had 58,100 colony forming units per milliliter (CFU/ml). Samples collected 1 and 2 days after cleaning had 250,200 and 528,160 CFU/ml, respectively. This demonstrates not only that the cleaning process is ineffective, biologically, it may increase the risk of bioaerosol contamination. Sample concentrations, in colony forming units per milliliter, were found to be quite different depending upon the side of the baffle plates from which the sample was collected. In some units the spray side of the water reservoir was visually clear with no observable microbial contamination. However, water on the backside (intake air side) of the reservoir would have a green, slimy and frothy contaminant floating on top of the water. This study demonstrates that gross contamination of microorganisms can exist in the water reservoirs of HVAC humidification systems. Burkhart, J.E.; Stanevich, R.; Kovak, B.: Microorganism Contamination of HVAC Humidification Systems: Case Study. Appl Occup. Environ. Hyg. 8(12):1010–1014; 1993.