To make the service facilities of Yunzhan expressway becoming the first carbon-neutral Expressway energy-saving and emission reduction demonstration service area in Guangdong Province, utilize the function of carbon sequestration and oxygen release of forest to reduce the carbon emissions of Expressway area and improve the atmospheric environment. Based on the investigation of plants in Yunzhan expressway Service Area of Guangdong Province, the carbon fixation and oxygen release capacity of plants in service area were investigated and evaluated by Li-6400 portable photosynthetic apparatus. It can be concluded that the ability of carbon fixation and oxygen release of plants is related to their own species structure, and is also closely related to the amount of plant green. Broad-leaved trees have large leaf area, so transplanted trees can play a greater role in carbon fixation and oxygen release.