Large Globules Research Articles

Light and electron microscopic immunocytochemistry of the pituitary gland of the tortoise.

The pars distalis of the tortoise, Geoclemis reevesii, was studied immunocytochemically with regards to its fine structure and distribution of different cell types. The pars distalis consists of cephalic and caudal lobes and a zona tuberalis, each with distinct cellular components. The cephalic lobe contains ACTH cells and prolactin (PRL) cells, while the caudal lobe consists of STH (GH) cells, TSH cells and FSH/LH (GTH) cells. The zona tuberalis contains GTH cells and a small number of TSH cells. ACTH cells, found exclusively in the cephalic lobe, are amphophilic cells containing ACTH immunoreactive secretory granules 200-240 nm in diameter. PRL cells occur only in the cephalic lobe and are large oval or columnar cells containing large secretory granules 300-500 nm in diameter. The cells immunoreactive to anti-chicken LH serum are also reactive to anti-chicken FSH serum, and were identified as GTH cells. They are distributed in the caudal lobe and zona tuberalis, with a few in the pars tuberalis. The GTH cells contain numerous secretory granules of varying density and diameter 200-280 nm, and large globules. TSH cells are small rounded cells containing small secretory granules 180-240 nm in diameter, and distributed in the caudal lobe and zona tuberalis. After thyroidectomy, these cells are hypertrophied and develop to thyroidectomy cells containing large vacuoles and a few secretory granules. GH cells are typical orangeophiles distributed in the caudal lobe. They contain secretory granules 200-400 nm in diameter. The zona tuberalis, which covers the antero-lateral portion of the cephalic lobe, is closely similar in cellular constitution to that of caudal lobe. It may presumably be a structure extended from the caudal lobe and not a homologue of the mammalian zona tuberalis.

Comparison of the fat content and fat globule size distribution of breast milk from mothers delivering term and preterm

The fat content and the fat globule size distribution of human preterm milk samples were analyzed and compared with data of previously analyzed samples of term milk. A negative correlation was found between the fat content and the gestational age. In both term and preterm milk three subpopulations of fat globules could be observed. The subpopulations of small (1 to 15 µm) and large globules (8 to 13 µm) were larger in preterm milk, but decreased slower throughout lactation. The average diameter was identical in both milks and increased with advancing lactation. The fat surface, which also depends on the fat content, increased with decreasing gestational age. However, no correlation between the specific fat surface area and the gestational age was found. Compared to human milk, commercial infant formulas have a smaller average fat globule diameter and a larger specific fat surface area.

Effect of 17 α‐Methyltestosterone on the Cytology of the Pituitary and the Liver, and on Plasma Electrolytes in Male Silver Eels

AbstractMale silver eels kept in fresh water were injected with 17α‐methyltestosterone (MT). They received 5, 10, 15 or 20 injections (8 μg/g every other day) or 6 or 9 injections (4 μg/g or 2.5 μg/g once a week). Compared to solvent‐injected controls, the treated eels showed changes in the pituitary: prolactin, corticotropic, thyrotropic and melanotropic cells were stimulated. Gonadotropic cells had enlarged nuclei, but synthesis and storage of granules and large globules were not evident, despite the progressive testicular maturation. High doses of MT induced a liver hypertrophy, a drop in plasma osmolarity and sodium level. An increase in plasma calcium level is possibly involved in the reduced activity of PAS‐positive Ca‐sensitive cells of the pars intermedia. These responses, previously observed in estradiol (E2) treated eels, may suggest that MT is aromatized in the brain and/or the pituitary. However, the considerable stimulation of GTH cells which always occurs in E2‐treated eels is absent in MT‐treated eels. With lower doses of MT, changes in the pituitary and the testis were similar to those produced by high doses, although the Ca‐sensitive cells were more active. The decrease in plasma osmolarity and sodium level was less marked, plasma calcium was slightly reduced and the liver was not modified. There is no clear evidence in favour of E2 production. These data suggest that MT may stimulate the gonad directly, in agreement with biochemical data by Dufour et al. (1983).

The egg-shell of Labiostrongylus eugenii (nematoda, strongyloidea): Structure and function

The egg-shell of Labio-strongylus eugenii consists of three layers; an outer vitelline layer, a middle chitinous layer and an inner lipid layer. The presence of chitin and protein in the middle layer was demonstrated by the use of chitinase and differential staining. The lipid layer was found to be made up of two layers, the innermost having large globules on its outer face. The shell was found to be permeable to liquid water, as demonstrated by the penetration of vital dyes. Eggs were able to develop normally with little or no loss of volume during periods of moisture stress when either osmotic or suction pressures were applied. The survival of eggs, as measured by hatching success, over periods of time at a range of saturation deficits and osmotic pressures was measured, and compared with known survival rates for other nematode species. The problems of working with nematode species whose life cycles have not been established are discussed. Possible survival strategies for Australian strongylids during periods of moisture stress are outlined.

Small intestinal mucosal fat in childhood enteropathies.

A sequential series of 100 small bowel mucosal biopsies from children was studied to assess the frequency and pattern of mucosal fat staining, and to compare patterns of fat distribution with mucosal structure and clinical diagnosis. Deep mucosal fat was commonly associated with those clinical groups showing normal mucosal structure. While fine granular surface epithelial fat was common in normal and abnormal biopsies, the presence of large fat globules in the surface epithelium was almost entirely limited to biopsies showing villous shortening. Large fat globules in the surface epithelium in coeliac disease and cow's milk sensitive enteropathy were probably related to the more severe degrees of villous abnormality encountered in these clinical groups. However, large fat globules in surface epithelium were also found in a few cases of cow's milk sensitive enteropathy with normal or minimal villous blunting. Fat staining may be a useful additional histological marker to aid in the interpretation of small intestinal mucosal biopsies.

The composition of a titanium slag from sorel

Sorelslag, which contains 71%–72% TiO 2, has almost the same chemical analysis and mineralogical composition as a mineral in the lunar rocks from the Apollo 11 and 17 landing sites. It is composed mainly of armalcolite (FeO·MgO·4TiO 2), a silicate phase containing trivalent titanium, tiny globules of metallic iron, which are formed by the reaction Fe 2+ + 2 Ti 3+→ Fe° + 2 Ti 4+, and a small number of large globules of metallic iron which are assumed to be entrained in the slag during tapping. These large globules have a rim of ferrous sulphide. When the amount of titanium(III) in the slag is high as a result of the addition of more carbon during the reduction of ilmenite, the product decrepitates on cooling owing to the enhancement of the above reaction. The residue obtained after baking the slag with concentrated H 2SO 4 and then leaching with water is mainly rutile associated with the silicate phase together with a small amount of unreacted slag.

The brain-pituitary-gonadal axis in the rainbow trout, Salmo gairdneri. II. Direct effect of gonadal steroids on the gonadotropic cells.

In a cytophysiological study it was investigated whether in juvenile trout gonadal steroids stimulate the gonadotropic (GTH)-cells directly or indirectly via the brain. Pituitaries of donor animals were transplanted into the caudal musculature of testosterone-treated and non-testosterone-treated host fish. Testosterone treatment caused an increase in GTH-content in the in situ pituitaries and in the grafts. Accordingly, the gonadotrops displayed ultrastructural changes such as the appearance of well-developed Golgi systems and large globules. The stimulation of the morphological development of gonadotrops and of synthesis and storage of GTH in the allografted pituitaries indicates that testosterone affects the GTH-cells directly. In untreated juvenile trout the gonadotropin content of the pituitary and the gonadotropin concentration in the plasma vary with the time of year. This variation and the role of testosterone and gonadotropin-releasing hormone on the release of GTH are discussed.

Vertebrate host specificity and experimental vectors of Plasmodium (Novyella) kempi sp. n. from the eastern wild turkey in Iowa.

Vertebrate host specificity, experimental laboratory vectors, and a description of Plasmodium (Novyella) kempi sp. n. from eastern wild turkeys (Meleagris gallopavo silvestris Vieillot) in Iowa are presented. Plasmodium kempi is infective for domestic turkeys, bobwhites (Colinus virginianus), chukars (Alectoris graeca), guinea fowl (Numida meleagris), peacocks (Pavo cristatus), and canaries (Serinus canaria), produces a transient infection in mallards (Anas platyrhynchos) and domestic geese (Anser anser), but will not infect ring-necked pheasants (Phasianus colchicus), pigeons (Columba livia), Japanese quail (Coturnix coturnix), leghorn white chickens (Gallus gallus), or starlings (Sturnus vulgaris). Oocysts and (or) sporozoites were recovered from 68% (84/124) and 98% (60/61) of the Culex pipiens pipiens and C. tarsalis examined, respectively. Oocysts developed faster and sporozoites invaded the salivary glands sooner in C. tarsalis (6 days) than in C. p. pipiens (7 days). Culex tarsalis transmitted P. kempi more effectively than C. p. pipiens, although both species were capable of transmitting the parasite by natural feeding. Oocysts developed and sporozoites also were produced in C. restuans, but its ability to transmit the parasite was not determined. Aedes aegypti (Rockefeller strain) and A. triseriatus were refractive to P. kempi. Plasmodium kempi produces trophozoites with large refractile globules and fine cytoplasmic extensions, mature schizonts in the form of a condensed fan containing four to eight nuclei (usually 5), and elongate gametocytes with irregular borders. All stages are confined almost exclusively to mature erythrocytes, with no effect on host cell size or position of host cell nucleus. Plasmodium kempi is most similar morphologically to P. (Novyella) hexamerium and P. (Novyella) vaughani. It differs from P. hexamerium in having large refractile globules in trophozoites and immature schizonts, an inability to infect starlings, an absence of phanerozoites in capillary endothelium of the brain, and the ability to develop in C. pipiens mosquitoes. Plasmodium kempi is more like P. vaughani morphologically, but differs by infecting turkeys and ducks (transient), by its inability to infect starlings, its lack of morphological variation even when in different hosts, and its ability to develop in C. pipiens and C. tarsalis.

Comparative Ultrastructure of the Compound Eye of the Wild, Laboratory-Reared, and Irradiated Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae)1

The compound eye of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), was found to consist of ca. 3,000 ommatidia, each composed of a dioptric apparatus overlying a cluster of eight retinula cells. A cross-section of the cell cluster reveals an open rhabdom structure with a superior R7 central cell and an inferior R8 central cell similar to that of other cyclorrhaphous Diptera. Each ommatidium has a pair of primary pigment cells and 12 shared longitudinal pigment cells. The dimensions and histology of all ommatidial components are described briefly. Various ultrastructural differences occur between wild and laboratory-reared flies. Laboratory-reared, particularly pupal-irradiated, flies have ommatidia comprised of less tightly packed retinula cells, and rhabdomeres that are consistently smaller, occasionally absent, and more peripherally distributed within the ommatidial cavity than those in wild flies. The R7 rhabdomere is sometimes expanded into a bulb at the level of the Rl-7 nuclei in laboratory-reared flies, but not in wild flies. Ommatidial cavities of laboratory-reared flies are enlarged and often contain inclusions not present in wild flies. The cytoplasmic density and concentrations of large lipid globules is reduced in the longitudinal pigment cells of laboratory-reared flies. The pattern of increasing ultrastructural deviation from the wild condition in laboratory-reared and irradiated flies is consistent with, and indeed provides a basis for, explaining a pattern of decreasing spectral sensitivity (wavelengths 350 to 750) found electroretinographically for flies obtained from the same samples.

In vitro studies of microwave-induced cataract: Separation of field and heating effects

A parametric study of heat and pulsed microwave energy was performed on murine ocular lens in vitro. Adult rat lenses were placed in a specially designed chamber with thermostat in which temperature was maintained by the circulation of thermostatically regulated phosphate-buffered saline, during irradiation with 915 MHz microwaves. Irradiation in pulsed (Pu) mode was used. Lenses exposed at 37°C to pulsed irradiation at specific absorption rates (SAR) equal to or greater than 400 mW/g, and immediately fixed, showed numerous small holes (not previously reported for other in vitro cataracts) in the equatorial cell surfaces, when examined by scanning electron microscopy (SEM). At 39°C with SAR equal to or greater than 120 mW/g similar holes appeared. On continued incubation for two days the holes could not be seen as easily because globular degeneration appeared to advance into the equatorial areas where holes had originally been seen. In lenses exposed to 120 mW/g at 37°C globular degeneration and equatorial subcapsular foam were observed. At SAR values equal to or above 400 mW/g at 39°C and 1·2 W/g at 37°C, large globules characteristic of temperature elevation to 47 or 50°C were observed, indicating that the effect of the electromagnetic field itself in the absence of any comparable temperature elevation, was equivalent to a 10°C rise in temperature. An unexpected finding was the apparent fragmentational stretching of the capsule observed by SEM, in lenses fixed immediately after irradiation at 37°C at an SAR value of 1·2 W/g. These effects: holes, foam, large globules and capsular damage are apparent examples of physical damage to the lens. Modern radars using directional antennas can deliver pulse power densities many times higher than those employed in these experiments. This work indicates that additional effects of microwave irradiation dependent on modulation should also be considered as potential hazards in setting safety standards.

Menstrual-cycle-dependent and -independent features of the human Fallopian tube fimbrial epithelium: an ultrastructural and cytochemical study.

Ultrastructural and cytochemical features of 100 specimens of human Fallopian tube fimbrial epithelium were studied to determine whether they were dependent on or independent of the ovarian hormones. Characteristics which were independent of hormonal control included the presence of alkaline phosphatase reaction product along the apical cell membrane of the secretory cell and its absence in the ciliary cell. Acid phosphatase reaction product was found in ciliary and secretory cell lysosomes in similar numbers and calcium deposits were observed in ciliary and secretory cell nuclei, nucleoli and cell membranes. On the other hand, characteristics dependent on stages of the cycle included the presence of mitochondrial-bound calcium during the preovulatory phase and its depletion during the periovulatory phase. The same sequence was noticed in the content of the large ciliary cell perinuclear globules, which displayed accumulations of glycogen and lipid in preovulatory phase and their depletion during the periovulatory phase. It seems that during the periovulatory phase the increased metabolic activity of the cells is accompanied by utilization of some of the material stored within the cells.

Coccidia of Brazilian edentates: Eimeria cyclopei n.sp. from the silky anteater, Cyclopes didactylus (Linn.) and Eimeria choloepi n.sp. from the two-toed sloth, Choloepus didactylus (Linn.)

Eimeria cyclopei n.sp. is described from the silky anteater, Cyclopes didactylus, from Para State, north Brazil. Undifferentiated oocysts, passed in the faeces, complete sporulation in seven days at 26 to 28°C. Oocysts are ellipsoidal to sub-spherical, with a mean size of 28.1 × 23.6 μm: the wall is ∼1.5 to 2.0 μm thick, apparently with an outer thin, colourless membrane and two inner, thicker, striated and yellowish layers. There is no micropyle, oocyst residuum or polar body. The mean measurements of sporocysts are 19.0 × 9.0 μm, and they are slightly asymmetrical, elongate pear-shape, with a plug-shaped Steida body projecting beyond the end of the sporocyst. Sporozoites are as long as or longer than the sporocysts: The sporocyst residuum is scattered between sporozoites in younger specimens and becomes condensed into rounded mass in older ones. The endogenous stages occur in the epithelial cells of the ileum, on the lumenal side of the host-cell nucleus. Uninucleate meront, microgamont and macrogamont precursors are recognizable morphologically. Mature meronts are ∼ 20.0 × 15.7 μm some produce 12 to 20 merozoites which are ∼ 8.7 × 2.0 μm, and others 10 to 26 merozoites which are ∼ 11.4 × 2.0 to 15.0 × 3.0 μm. Mature microgamonts which are ∼27.5 × 24.1 μm, produce from 150 to 170 microgametes of ∼7.1 × 1.0 μm: microgametes have two flagella of unequal length. Mature macrogamonts are ∼ 28.4 × 24.5 μm Eimeria choloepi n.sp. is recorded from the two-toed sloth, Choloepus didactylus, from the same area of Brazil. Undifferentiated oocysts, passed in the faeces, complete sporulation in 23 days at 26 to 28°C. Oocysts with a mean size of 23.0 × 20.3 μm, have a wall ∼2.0 to 2.5 μm thick which is composed of two thick, yellowish and striated outer layers and a delicate, colourless inner one. There is no micropyle, oocyst residuum or polar granule. Mature sporocysts with a mean size of 11.3 × 7.1 μm, are ellipsoidal to egg-shaped and have a poorly developed Steida body. The sporocyst residuum is composed of a small number of large globules: The sporozoites are longer than the sporocyst and strongly recurved. The endogenous stages occur in epithelial cells of the ileum, on the lumenal side of the host-cell nucleus. Dimorphic meronts produce 8 to 18 merozoites which are either ∼13.0 × 2.0 μm or ∼13.0 × 3.0 μm. Microgamonts produce 50 to 80 microgametes of ∼8.0 × 1.0 μm. Mature macrogamonts are ∼18.3 × 17.9 μm. ac]19820212

Juxtanuclear globule of the human fimbrial epithelium ciliary cell: a histo- and cytochemical study.

Microbiopsies of human Fallopian tube fimbrial epithelium were obtained during routine gynecological operations, and during pregnancy while undergoing hysterectomy in the first trimester or cesarean section in the third trimester. Specimens were studied under light and electron microscopes utilizing histo- and cytochemical techniques. Large juxtanuclear globules were demonstrated in the ciliary cell at all stages of the cycle. During the proliferative phase, these globules contained large lipid droplets and glycogen rosettes. The content of the globules in the early luteal phase was mostly amorphous electron-lucid material which failed to stain with PAS, alcian blue and the Millon reaction. However, reaction products of acid phosphatase as well as calcium precipitate were scattered in them as discrete granules. The same type of globule was found in early pregnancy. During late luteal phase and late pregnancy, the content of the globule was once again composed of lipids and glycogen. It seems that lipid and glycogen accumulate during the preovulatory phase, while depletion and dispersion of these substances occur in the periovulatory phase, probably due to increased energy utilization. Late luteal phase revealed subsequent replenishment of these reserves, as was also revealed in late pregnancy.

Structure and development of plastids in hypsophylls of Euphorbia pulcherrima and Bouganwillea sp.

The fine structure of plastids in hypsophyll at different stages of development and pigmentation has been followed in two short-days plants: Euphorbia pulcherrima and Bouganwillea.In Euphorbia the young chloroplasts observed in the embryonal green hypsophyll change into plastids of small dimensions when contained in the red expanded hypsophyll. Moreover in the green leaves, where red colour is afterwards superimposing, chloroplasts are undergoing a degenerative process. In Bouganwillea the plastid ultrastructure is changing from a young embryonal feature to a moderately developed state, while in the fully expanded hypsophyll degenerated chloroplasts are present together with a population of plastids characterized by numerous large globules and few residual membranes. The unlike chloroplasts behaviour observed in the two different hypsophylls is discussed keeping in consideration pigment synthesis and hypsophyll derivation.In Bouganwillea phloem tubes characteristic P-plastids have been observed and have confirmed the importance of this plastid as a systematic character of Caryophyllales.

Human fallopian tube epithelium cytochemistry in the first and third trimesters of pregnancy.

The human Fallopian tube fimbrial epithelium was histo- and cytochemically studied in the first and third trimesters of pregnancy. In the first trimester morphometric measurements confirmed a decrease in cell size and a reduction in ciliary cell count. The ciliary cell population was surprisingly glycogen-poor, and some of the cell contained, among normal organelles, lipid accumulation, lamellated bodies and nuclei with slightly condensed chromatin. Some had defective cilia and basal bodies. Large globules, containing few lipid droplets and glycogen rosettes were demonstrated. The bulk of the globules, however, contained an unidentified electron-lucid substance, negative to PAS, Alcian blue, OsO4 and the Millon reactions, but positive to acid phosphatase reaction, the products of which appeared dispersed. The nonciliary cell population was heterogeneous; some appeared almost devoid of microvilli and had dilated RER and SER cisternae, lamellated bodies and condensed nuclear chromatin. In some cells a single centrally located cilium was noticed. Other nonciliary cells had extensive microvilli and appeared less atrophied. In the third trimester morphometric, as well as cytochemical methods indicated that the regressive process was beginning to reverse itself. Alkaline-phosphatase reaction products appeared along the apical and lateral membranes of the nonciliary cell. Acid-phosphatase reaction products depicted the lysosomes, present in both types of cells.

Electron microscopic and histochemical study of the postovulatory follicles of Perca fluviatilis L. (Teleostei)

Postovulatory follicles of Perca fluviatilis L. have been studied with histochemical, light, and electron microscopic methods. They show two antagonistic transformations: Some of their cells undergo a process of desintegration and autophagocytosis. This process resorbs cell debris left behind after ovulation, and also reduces the size of the follicle. Other cells of the follicular epithelium transform into synthetically active cells. This process can be divided into four stages: reorganization phase, vacuolar phase, globular phase, and regression phase. During the vacuolar and early globular phase the granulosa cells exhibit a well-developed agranular endoplasmic reticulum, mitochondria with tubular cristae, and a 3β-hydroxysteroid dehydrogenase activity. Large compound globules up to 15 μm in diameter, containing proteins and cholesterol-related material, accumulate within granulosa cells of the globular phase. Their exact chemical nature has yet to be determined. They seem to be shed as a holocrine secretion into the follicular lumen. The cells of the theca do not participate in this process. The regression phase is characterized by a vacuolar degeneration of the granulosa cells remaining after secretion, without formation of yellow pigments.

The fat globule size distribution in human milk

The size distribution of fat globules in human colostrum and milk was measured using a conductometric method. The number-, surface- and volume-frequency functions were analyzed and various parameters related to the stability of the polydisperse fat emulsion were calculated. The shape of the distribution curves suggests the presence of sub-populations of differently sized particles. A postulated sub-population of small globules with a size frequency maximum below 1 μm comprises 70–90% of the number of globules but only a few percent of the fat volume. A sub-population of medium-sized globules with a size frequency maximum around 4 μm comprises the largest amount of the fat. A shoulder in the distribution curves around 8–12 μm indicates a third population of large globules. They contribute about 0.01% to the number of fat globules but represent 1–4% of the milk fat. The height but not the position of the maxima of the sub-distributions changed throughout lactation. This affected the size-related parameters such as the overall mean globule diameter. The volume/surface average diameter increased from about 1.8 μm in colostrum to 4.0 μm after 4–5 months of lactation. The range of globule size is similar in human and cow's milk and milk of some other species. Compared to cow's milk human milk contains more small fat globules, especially at the earlier stages of lactation.

SYNGAMY AND RESTING BODY DEVELOPMENT IN CHYTRIOMYCES HYALINUS (CHYTRIDIALES)

Somatogamy by rhizoidal anastomosis initiates sexual reproduction and resting body formation in Chytriomyces hyalinus Karling. At least two external factors influence somatogamy: the level of nutrients available and the chance association of rhizoids of young thalli (germlings). Syngamy and resting body development occurs in axenic culture when germlings are developing on a nutrient‐depleted substrate and when the rhizoids of two germlings (contributing thalli) anastomose. After anastomosis, the contents of the two contributing thalli flow into a swelling (incipient fusion body) which develops near the point of anastomosis. Nuclear fusion occurs in the fusion body (incipient resting body). After nuclear fusion, the resting body continues to enlarge, its wall thickens, and lipoid inclusions coalesce to form a large refractile globule. This sexual process is better referred to as somatogamy rather than Siphonaria‐type sexual reproduction.

Syngamy and Resting Body Development in Chytriomyces hyalinus (Chytridiales)

Somatogamy by rhizoidal anastomosis initiates sexual reproduction and resting body formation in Chytriomyces hyalinus Karling. At least two external factors influence somatogamy: the level of nutrients available and the chance association of rhizoids of young thalli (germlings). Syngamy and resting body development occurs in axenic culture when germlings are developing on a nutrient-depleted substrate and when the rhizoids of two germlings (contributing thalli) anastomose. After anastomosis, the contents of the two contributing thalli flow into a swelling (incipient fusion body) which develops near the point of anastomosis. Nuclear fusion occurs in the fusion body (incipient resting body). After nuclear fusion, the resting body continues to enlarge, its wall thickens, and lipoid inclusions coalesce to form a large refractile globule. This sexual process is better referred to as somatogamy rather than Siphonaria-type sexual reproduction.

Feinstrukturelle Untersuchungen zur Entwicklung der Ölzellen bei dem LebermoosRiella

Liverworts are characterized by the possession of typical cell elements, the oil bodies. In the submerse, thalloid liverwortRiella helicophylla (Sphaerocarpales) oil bodies are existing in idioblastic cells only, called oil body cells. Each oil body cell contains only one oil body. The oil body originates from small vacuoles. Their membranes are extremely high in contrast and asymmetric. The lipophilic substances are probably produced inside the oil bodies. At the end of the development of an oil body cell lipophilic and hydrophilic material will be separated from each other inside the oil body. The result is an oil body, consisting of one large spherical oil globule surrounded by a thin layer of hydrophilic matrix.