Large Felids Research Articles

Caching Behavior of Large Prey by Eurasian Lynx: Quantifying the Anti-Scavenging Benefits

Large solitary felids often kill large prey items that can provide multiple meals. However, being able to utilize these multiple meals requires that they can cache the meat in a manner that delays its discovery by vertebrate and invertebrate scavengers. Covering the kill with vegetation and snow is a commonly observed strategy among felids. This study investigates the utility of this strategy using observational data from Eurasian lynx (Lynx lynx)-killed roe deer (Capreolus capreolus) carcasses, and a set of two experiments focused on vertebrate and invertebrate scavengers, respectively. Lynx-killed roe deer that were covered by snow or vegetation were less likely to have been visited by scavengers. Experimentally-deployed video-monitored roe deer carcasses had significantly longer time prior to discovery by avian scavengers when covered with vegetation. Carcass parts placed in cages that excluded vertebrate scavengers had delayed invertebrate activity when covered with vegetation. All three datasets indicated that covering a kill was a successful caching/anti-scavenger strategy. These results can help explain why lynx functional responses reach plateaus at relatively low kill rates. The success of this anti-scavenging behavior therefore has clear effects on the dynamics of a predator–prey system.

Suspected Sunda clouded leopard (Neofelis diardi) predation attempts on two reintroduced Bornean orangutans (Pongo pygmaeus wurmbii) in Bukit Batikap Protection Forest, Central Kalimantan, Indonesia.

In February 2017 and August 2018, respectively, two Bornean orangutans (Pongo pygmaeus wurmbii) reintroduced into the Bukit Batikap Protection Forest in Central Kalimantan were found in weakened physical condition and with deep puncture wounds. The first individual was a sub-adult male, and the second an adult female whose 6- to 8-week-old infant was missing. Both individuals were rescued and transported back to the field base camp for treatment. Experienced veterinarians treating the injuries reported that the type of wounds appeared consistent with those expected from an attack by a large felid. The Sunda clouded leopard (Neofelis diardi) is the largest felid known to inhabit Bukit Batikap Protection Forest, and we suspect that these cases were unsuccessful predatory attacks by this species. Given the severity of his condition when found, the male orangutan would probably have died without medical intervention; however, both orangutans fully recovered following intensive treatment and were successfully returned to the forest. Predation attempts on orangutans are infrequently reported, thus our observations add to the body of knowledge about possible predation by clouded leopards on reintroduced, rehabilitant orangutans.

The Gene Catalog and Comparative Analysis of Gut Microbiome of Big Cats Provide New Insights on Panthera Species.

Majority of metagenomic studies in the last decade have focused on revealing the gut microbiomes of humans, rodents, and ruminants; however, the gut microbiome and genic information (gene catalog) of large felids such as Panthera species are largely unknown to date. In this study, the gut bacterial, fungal, and viral metagenomic composition was assessed from three Panthera species (lion, leopard, and tiger) of Indian origin, which were consuming the same diet and belonged to the same geographical location. A non-redundant bacterial gene catalog of the Panthera gut consisting of 1,507,035 putative genes was constructed from 27 Panthera individuals, which revealed a higher abundance of purine metabolism genes correlating with their purine-rich dietary intake. Analysis with Carbohydrate Active enZyme (CAZy) and MEROPS databases identified enrichment of glycoside hydrolases (GHs), glycoside-transferases, and collagenases in the gut, which are important for nutrient acquisition from animal biomass. The bacterial, fungal, and viral community analysis provided the first comprehensive insights into the Panthera-specific microbial community. The Panthera gene catalog and the largest comparative study of the gut bacterial composition of 68 individuals of Carnivora species from different geographical locations and diet underscore the role of diet and geography in shaping the Panthera gut microbiome, which is significant for the health and conservation management of these highly endangered species.

Size Matters: Zoo Data Analysis Shows that the White Blood Cell Ratio Differs between Large and Small Felids.

The total number of white blood cells (WBCs) is related the immune system. In mammals, it is affected by the body mass, but it is unclear how the numbers of different WBC types correlate with this parameter. We analyzed the effect of body mass on WBC number and ratio in felids, where species are similar in diet (warm-blood vertebrates) and reproductive strategy (promiscuity). Based on zoo veterinary data (ZIMS database) we analyzed the effect of body mass on WBC number and neutrophils/lymphocytes ratio in 26 species of felids. The number of WBCs correlated with the body masses of animals: large cats had more WBC, which may be due to greater risks of infection associated with larger body surface, lifespan and home range size. For the first time we found obvious differences in the number of WBC types. Large cats also had more neutrophils and monocytes but fewer lymphocytes than smaller cats. The ratio of neutrophils to lymphocytes is greater in large felids. This phenomenon may be related to diet (relative prey size and kill utilization time), which suggests regular contact of large cats with bacterial and protozoal pathogens in contrast to the small cats.

Presença do ossículo meniscal na articulação femorotibial de gatos-domésticos (Felis catus, Linnaeus, 1758)

A articulação femorotibial é uma articulação complexa do tipo sinovial, que, além da relação com a patela, em algumas espécies de animais pode apresentar uma estrutura radiopaca visualizada ao exame radiográfico de formato oval entre os ossos do fêmur e tíbia, em projeção mediolateral, denominado ossículo meniscal. Embora comumente relatado em diferentes espécies, inclusive no homem, pouco se sabe sobre a sua função e frequência, sendo bem constatada sua existência em espécimes de grandes felídeos. O presente estudo teve como objetivo realizar a análise radiográfica da articulação femorotibiopatelar de 40 gatos-domésticos (Felis catus, Linnaeus, 1758) em projeções mediolateral e craniocaudal no intuito de avaliar a presença ou ausência do ossículo meniscal. Nas radiografias realizadas, o ossículo esteve presente em 15% (n = 6), observando-se sua maior frequência em machos e unilateralmente, sendo o joelho esquerdo o de maior incidência da estrutura. Por ser um achado comum e relatado em diversas espécies selvagens de felídeos, a presença do ossículo meniscal deve ser levada em consideração e inserida como diagnóstico diferencial quando da realização de radiografias de gatos-domésticos e da visualização de opacidade intra-articular em projeções radiográficas mediolaterais da articulação femorotibiopatelar.

Prey selection and food habits of the Tiger Panthera tigris (Mammalia: Carnivora: Felidae) in Kalakkad-Mundanthurai Tiger Reserve, southern Western Ghats, India

The Endangered Tiger Panthera tigris is the largest felid, distributed over 1.1 million km2 globally. Conservation of Tigers largely depends on the preservation of its natural prey base and habitats. Therefore, the availability of prey and its selection play a major role in the sustainable future of Tigers in the given landscape. The current study assesses the prey selection patterns by Tigers in tropical evergreen forest of the Kalakkad-Mundanthurai Tiger Reserve (KMTR), southern Western Ghats, India. Density of ungulates was assessed by distance sampling (line transect, N = 21) and diet composition of Tigers was evaluated by analysing their faecal samples (N = 66). The study estimated very low ungulate density (26.87 ± 7.41 individuals km-2) with highest density of Gaur Bos gaurus (9.04 individuals km-2) followed by Wild Boar Sus scrofa (8.79 ± 2.73 individuals km-2), whereas, primate density was quite high (45.89 ± 12.48 individuals km-2), with Nilgiri Langur Semnopithecus johnii having the highest density (38.05 ± 10.22 individuals km-2). About 74.62% of the biomass of Gaur constituted in the Tiger’s diet, consumed lesser than its availability, whereas Sambar constituted 16.73% of the Tiger diet consumed proportionally to its availability. Chital Axis axis, Muntjac Muntiacus muntjak, and Indian Chevrotain Moschiola indica were not represented in the Tiger’s diet. The current study is the first scientific information on prey selection of the Tiger in KMTR landscape, which will serve as a baseline for its conservation planning and management.

Taiwanese attitudes toward the clouded leopard (Neofelis nebulosa) and its potential reintroduction

ABSTRACT Social acceptance is crucial to reintroduction projects involving large felids, yet few studies have assessed public attitudes toward reintroduction candidates prior to release. To assess attitudes toward clouded leopards, a reintroduction candidate in Taiwan, we conducted interviews with 263 rural indigenous locals in southern Taiwan and distributed an internet questionnaire to 500 Taiwanese urbanites. Rural and urban attitudes were 67% and 76% positive toward clouded leopards, respectively. Higher knowledge about clouded leopards, male respondents, and younger-aged respondents were positively correlated with rural attitudes toward clouded leopards. Comparably, an increase in knowledge, male respondents, and those with more household children were positively associated with urban attitudes. Additionally, 67% of rural and 71% of urban respondents exhibited positive attitudes toward clouded leopard competitors and prey in Taiwan, respectively. Our findings suggest that clouded leopard reintroduction may already be well-supported among members of Taiwanese indigenous nations and more broadly by the general public.

Understanding decision making in a food-caching predator using hidden Markov models

BackgroundTackling behavioural questions often requires identifying points in space and time where animals make decisions and linking these to environmental variables. State-space modeling is useful for analysing movement trajectories, particularly with hidden Markov models (HMM). Yet importantly, the ontogeny of underlying (unobservable) behavioural states revealed by the HMMs has rarely been verified in the field.MethodsUsing hidden Markov models of individual movement from animal location, biotelemetry, and environmental data, we explored multistate behaviour and the effect of associated intrinsic and extrinsic drivers across life stages. We also decomposed the activity budgets of different movement states at two general and caching phases. The latter - defined as the period following a kill which likely involves the caching of uneaten prey - was subsequently confirmed by field inspections. We applied this method to GPS relocation data of a caching predator, Persian leopard Panthera pardus saxicolor in northeastern Iran.ResultsMultistate modeling provided strong evidence for an effect of life stage on the behavioural states and their associated time budget. Although environmental covariates (ambient temperature and diel period) and ecological outcomes (predation) affected behavioural states in non-resident leopards, the response in resident leopards was not clear, except that temporal patterns were consistent with a crepuscular and nocturnal movement pattern. Resident leopards adopt an energetically more costly mobile behaviour for most of their time while non-residents shift their behavioural states from high energetic expenditure states to energetically less costly encamped behaviour for most of their time, which is likely to be a risk avoidance strategy against conspecifics or humans.ConclusionsThis study demonstrates that plasticity in predator behaviour depending on life stage may tackle a trade-off between successful predation and avoiding the risks associated with conspecifics, human presence and maintaining home range. Range residency in territorial predators is energetically demanding and can outweigh the predator’s response to intrinsic and extrinsic variables such as thermoregulation or foraging needs. Our approach provides an insight into spatial behavior and decision making of leopards, and other large felids in rugged landscapes through the application of the HMMs in movement ecology.

Genetic analyses reveal population structure and recent decline in leopards (Panthera pardus fusca) across the Indian subcontinent.

BackgroundLarge carnivores maintain the stability and functioning of ecosystems. Currently, many carnivore species face declining population sizes due to natural and anthropogenic pressures. The leopard, Panthera pardus, is probably the most widely distributed and highly adaptable large felid globally, still persisting in most of its historic range. However, we lack subspecies-level data on country or regional scale on population trends, as ecological monitoring approaches are difficult to apply on such wide-ranging species. We used genetic data from leopards sampled across the Indian subcontinent to investigate population structure and patterns of demographic decline.MethodsWe collected faecal samples from the Terai-Arc landscape of northern India and identified 56 unique individuals using a panel of 13 microsatellite markers. We merged this data with already available 143 leopard individuals and assessed genetic structure at country scale. Subsequently, we investigated the demographic history of each identified subpopulations and compared genetic decline analyses with countrywide local extinction probabilities.ResultsOur genetic analyses revealed four distinct subpopulations corresponding to Western Ghats, Deccan Plateau-Semi Arid, Shivalik and Terai region of the north Indian landscape, each with high genetic variation. Coalescent simulations with microsatellite loci revealed a possibly human-induced 75–90% population decline between ∼120–200 years ago across India. Population-specific estimates of genetic decline are in concordance with ecological estimates of local extinction probabilities in these subpopulations obtained from occupancy modeling of the historic and current distribution of leopards in India.ConclusionsOur results confirm the population decline of a widely distributed, adaptable large carnivore. We re-iterate the relevance of indirect genetic methods for such species in conjunction with occupancy assessment and recommend that detailed, landscape-level ecological studies on leopard populations are critical to future conservation efforts. Our approaches and inference are relevant to other widely distributed, seemingly unaffected carnivores such as the leopard.

Large carnivores as potential predators of sun bears

Sun bears (Helarctos malayanus) have a wide distribution in Southeast Asia, but little is known about their natural predators. During a camera-trap survey in 2018 in Htamanthi Wildlife Sanctuary, Myanmar, we photographed a male leopard (Panthera pardus) carrying a sun bear cub by the throat. This is the first reported case of probable predation on sun bears by leopards, and only their second confirmed predator. A literature review showed that consumption of sun bears and Asiatic black bears (Ursus thibetanus) by tigers (P. tigris) was widespread in Southeast Asia, whereas consumption of both bear species by leopards and dholes (Cuon alpinus) was less common. Outside of Southeast Asia, tigers and leopards, but not dholes, were shown to kill or consume other bear species. Future research should examine interspecific relationships between sun bears and large felids to better understand what, if any, impacts large felids have on sun bear ecology.

REVIEW OF CANINE DISTEMPER VACCINATION USE AND SAFETY IN NORTH AMERICAN CAPTIVE LARGE FELIDS (PANTHERA SPP.) FROM 2000 TO 2017.

Data on canine distemper virus (CDV) vaccination were collected on 812 large felids (351 tigers, Panthera tigris; 220 lions, Panthera leo; 143 snow leopards, Panthera uncia; 50 leopards, Panthera pardus; and 48 jaguars, Panthera onca) from 48 institutions to assess vaccine use and safety. The documented individual vaccination events with multiple products numbered 2,846. Canarypox-vectored CDV vaccines were the most commonly used vaccines (96.3% of all vaccinations) and the Purevax® Ferret Distemper (PFD) vaccine was the most commonly used canarypox-vectored vaccine (91.0% of all vaccinations). Modified live virus (MLV) CDV vaccines were used for 3.7% of all vaccinations, and only in tigers, lions, and snow leopards. Adverse effects were reported after 0.5% (13 of 2,740) of the canarypox-vectored vaccinations and after 2.9% (3 of 104) of the MLV CDV vaccinations. This low complication rate suggests large felids may not be as sensitive to adverse effects of MLV CDV vaccines as other exotic carnivores. Serological data were available from 159 individuals (69 tigers, 31 lions, 31 snow leopards, 22 jaguars, and 6 Amur leopards, Panthera pardus orientalis) vaccinated with the PFD vaccine, and 66.0% of vaccinates seroconverted (defined as acquiring a titer ≥1: 24) at some point postvaccination: 24.3% after one vaccination, 55.8% after two vaccinations, 54.3% after three vaccinations, and 79.2% after four or more vaccinations. Among animals exhibiting seroconversion after the initial PFD vaccinations, 88.9% still had titers ≥12 mo and ≥24 mo after the last vaccination, and 87.5% had titers ≥1: 24 at ≥36 mo after the last vaccination. The study was unable to assess fully the safety of vaccination with either canarypox-vectored or MLV CDV vaccines during gestation because of the small number of animals vaccinated while pregnant (n = 6, all vaccinated with PFD).

Tratamento cirúrgico da obstrução intestinal por fecaloma em um puma (Puma concolor)

The puma (Puma concolor) is a large felid native to the Americas and one of the endangered species of the Brazilian fauna. Digestive disorders such as fecalomas are common in veterinary practice. Fecalomas are masses of retained feces that accumulate in the large colon and progress to hard fecal concretions that are difficult and painful to expel. Inadequate diets and mechanical intraluminal or extraluminal intestinal obstructions are the major causes of fecaloma. Tenesmus, anorexia, vomiting and dehydration are common clinical signs. The diagnosis is based on medical history and physical examination, and can be confirmed by imaging assessment. Fecalomas are amenable to conservative and surgical treatment, depending on disease severity. This paper describes a case of fecaloma in a puma. The diagnosis was made during exploratory laparotomy and improper diet incriminated as the cause. Surgical intervention consisted of enterotomy followed by removal hardened retained feces. Postoperative dietary management consisted of a liquid follow by a soft diet, with reintroduction of the regular diet after full recovery. Dietary supplementation with vegetable fibers was prescribed for life to prevent recurrence.

Food from faeces: Evaluating the efficacy of scat DNA metabarcoding in dietary analyses.

Scat DNA metabarcoding is increasingly being used to track the feeding ecology of elusive wildlife species. This approach has greatly increased the resolution and detection success of prey items contained in scats when compared with other classical methods. However, there have been few studies that have systematically tested the applicability and reliability of this approach to study the diet of large felids species in the wild. Here we assessed the effectiveness of this approach in the cheetah Acinonyx jubatus. We tested how scat degradation, meal size, prey species consumed and feeding day (the day a particular prey was consumed) influenced prey DNA detection success in captive cheetahs. We demonstrated that it is possible to obtain diet information from 60-day old scats using genetic approaches, but the efficiency decreased over time. Probability of species-identification was highest for food items consumed one day prior to scat collection and the probability of being able to identify the species consumed increased with the proportion of the prey consumed. Detection success varied among prey species but not by individual cheetah. Identification of prey species using DNA detection methods from a single consumption event worked for samples collected between 8 and 72 hours post-feeding. Our approach confirms the utility of genetic approaches to identify prey species in scats and highlight the need to account for the systematic bias in results to control for possible scat degradation, feeding day, meal size and prey species consumed especially in the wild-collected scats.

Vertical relief facilitates spatial segregation of a high density large carnivore population

Quantifying the distribution and size of home ranges is critical for understanding animal spatial dynamics. This is particularly important for large carnivores in fragmented landscapes. Most studies that estimate home range consider only a bivariate frequency distribution represented by a two‐dimensional planimetric surface. The underlying assumption of these approaches is that the animals inhabit landscapes that are completely flat. Of course, this is rarely the case. Here we investigated the influence of vertical relief and three‐dimensional landscape features on the home range patterns of a high density carnivore. Via GPS telemetry‐tracking of a population of Persian leopards Panthera pardus saxicolor (n = 6), and globally‐available digital elevation models (DEMs), we calculated the surface area of home ranges in comparison to traditional planimetric estimates. We also investigated predation patterns of leopards across elevation gradients using GPS location data and kill site analysis. The topographic measurements exceeded planimetric estimates by up to 38% which suggests that planimetric modeling underestimates home range size, particularly when animals inhabit variable terrain. We also observed that resident leopards exhibit significant altitudinal partitioning of predation, suggesting that leopards that have overlapping home ranges may still utilize exclusive hunting territories. We discuss the ways in which planimetric approaches may be underestimating aspects of animal ranging behavior and ecology. We conclude that topography should be considered, not as an ancillary metric, but as an important aspect of home range calculation. Our approach can enhance understanding of spatial requirements, population density, intra‐guild sympatric competition and conflict management of large felids inhabiting rugged landscapes.

Visual encounters on line transect surveys under-detect carnivore species: Implications for assessing distribution and conservation status.

We compared the distribution and occurrence of 15 carnivore species with data collected monthly over three years by trained native trackers using both sign surveys and an encounter-based, visual-distance method in a well-preserved region of southern Guyana (Amazon / Guiana Shield). We found that a rigorously applied sign-based method was sufficient to describe the status of most carnivore species populations, including rare species such as jaguar and bush dog. We also found that even when accumulation curves for direct visual encounter data reached an asymptote, customarily an indication that sufficient sampling has occurred to describe populations, animal occurrence and distribution were grossly underestimated relative to the results of sign data. While other researchers have also found that sign are better than encounters or camera traps for large felids, our results are important in documenting the failure of even intensive levels of effort to raise encounter rates sufficiently to enable statistical analysis, and in describing the relationship between encounter and sign data for an entire community of carnivores including felids, canids, procyonids, and mustelids.

Late Pleistocene records of felids from Medicine Hat, Alberta, including the first Canadian record of the sabre-toothed cat Smilodon fatalis

In the late 1960s, a team led by C.S. Churcher and A. MacS. Stalker collected over 1000 vertebrate fossils, mostly representing large herbivorous mammals, from bluffs along the South Saskatchewan River near Medicine Hat, Alberta, Canada. The records from this area also include the only documented case of the sabre-toothed cat Smilodon fatalis, but these specimens have not been described or illustrated, and therefore, their identification has never been verified. Here, all felid fossils recovered from the Medicine Hat bluffs are described and identified. We confirm the presence of the machairodontine S. fatalis and three additional taxa: the feline Lynx and the pantherines Panthera cf. P. atrox (American lion) and Panthera cf. P. spelaea (cave lion). Notably, this record of S. fatalis is its first confirmed occurrence in Canada and is a significant northerly range expansion, bringing the global distribution of this species in line with what is typical for a large felid. Should the tentative record of Panthera cf. P. spelaea be correct, this would represent its first occurrence in Alberta and a southeastern range extension, bringing it into the range of P. atrox. The possible presence of both P. atrox and P. spelaea suggests that Late Pleistocene pantherine biogeography in North America may be more complex than previously believed, particularly during relatively warm interglacial periods.

Documenting the demise of tiger and leopard, and the status of other carnivores and prey, in Lao PDR's most prized protected area: Nam Et - Phou Louey

The Nam Et - Phou Louey National Protected Area (NEPL) is known for its diverse community of carnivores, and a decade ago was identified as an important source site for tiger conservation in Southeast Asia. However, there are reasons for concern that the status of this high priority diverse community has deteriorated, making the need for updated information urgent. This study assesses the current diversity of mammals and birds in NEPL, based on camera trap surveys from 2013 to 2017, facilitating an assessment of protected area management to date. We implemented a dynamic multispecies occupancy model fit in a Bayesian framework to reveal community and species occupancy and diversity. We detected 43 different mammal and bird species, but failed to detect leopard Panthera pardus and only detected two individual tigers Panthera tigris, both in 2013, suggesting that both large felids are now extirpated from NEPL, and presumably also more widely throughout Lao PDR. Mainland clouded leopard Neofelis nebulosa had the highest estimates of probability of initial occupancy, persistence and colonization, and appeared to be the most widely distributed large carnivore, followed by dhole Cuon alpinus. Both of these species emerge as a priority for further monitoring and conservation in the NEPL landscape. This study provides the most recent assessment of animal diversity and status in the NEPL. Our analytical approach provides a robust and flexible framework to include sparse and inconsistent data sets of multiple species to assess their status via occupancy as a state process, which can often provide insights into population dynamics.

Taphonomic and zooarchaeological investigations at the middle Pleistocene site of Ti's al Ghadah, western Nefud Desert, Saudi Arabia

In recent years, the Arabian Peninsula has emerged as a key region for elucidating hominin and faunal evolution and dispersals between Africa and Eurasia. Central to this research is the middle Pleistocene site of Ti's al Ghadah (TAG) which has yielded a diverse and abundant fossil faunal assemblage and the earliest chronometrically dated evidence for hominins in this part of the world. Here, we present the first detailed taphonomic study of the large Unit 5 fossil assemblage from the site. We aim to assess which actor/s were responsible for the accumulation of the assemblage and evaluate evidence that might be consistent with the accumulation of fauna by hominins. We also describe, for the first time, fossils and lithic artefacts from stratigraphic horizons not previously considered, providing taphonomic insights into their accumulation. The taphonomic work shows that the Unit 5 faunal assemblage was accumulated by ambush predators, likely large felids and hominins, in a lake side environment, and that carcasses were subsequently scavenged by more durophagus carnivores such as hyenas and canids. Less can be reliably said regarding the newly described fossil assemblages given their poor preservation and significant wind abrasion, but large carnivores again appear to have played a role, and hominins probably played a role in the accumulation of at least one of these. This study provides the first detail insights into the interplay between hominins, carnivores, and herbivores in Arabia, and suggests that watering holes have been a focus on the Arabian landscape for resources since the middle Pleistocene.

Geographical assignment of polar bears using multi-element isoscapes

Wide-ranging apex predators are among the most challenging of all fauna to conserve and manage. This is especially true of the polar bear (Ursus maritimus), an iconic predator that is hunted in Canada and threatened by global climate change. We used combinations of stable isotopes (13C,15N,2H,18O) in polar bear hair from > 1000 individuals, sampled from across much of the Canadian Arctic and sub-Arctic, to test the ability of stable isotopic profiles to ‘assign’ bears to (1) predefined managed subpopulations, (2) subpopulations defined by similarities in stable isotope values using quadratic discriminant analysis, and (3) spatially explicit, isotopically distinct clusters derived from interpolated (i.e. ‘kriged’) isotopic landscapes, or ‘isoscapes’, using the partitioning around medoids algorithm. A four-isotope solution provided the highest overall assignment accuracies (~80%) to pre-existing management subpopulations with accuracy rates ranging from ~30–99% (median = 64%). Assignment accuracies of bears to hierarchically clustered ecological groups based on isotopes ranged from ~64–99%. Multivariate assignment to isotopic clusters resulted in highest assignment accuracies of 68% (33–77%), 84% (47–96%) and 74% (53–85%) using two, three and four stable isotope groups, respectively. The resulting spatial structure inherent in the multiple stable isotopic compositions of polar bear tissues is a powerful forensic tool that will, in this case, contribute to the conservation and management of this species. Currently, it is unclear what is driving these robust isotopic patterns and future research is needed to evaluate the processes behind the pattern. Nonetheless, our isotopic approach can be further applied to other apex mammalian predators under threat, such as the large felids, providing that isotopic structure occurs throughout their range.

Inter-dependent movements of Asiatic Cheetahs Acinonyx jubatus venaticus and a Persian Leopard Panthera pardus saxicolor in a desert environment in Iran (Mammalia: Felidae)

We investigated the simultaneous and sympatric movements of a coalition of two Asiatic Cheetahs (Acinonyx jubatus venaticus) and a Persian Leopard (Panthera pardus saxicolor), two rare and highly mobile large felids in Bafq Protected Area, Iran. The animals were tracked with GPS collars for 4.5 to 9 months at a temporal resolution of eight hours. The cheetahs used lower elevations areas (average: 1600 m), and remained more distant to the surrounding highways of (average: 14.5 km) than the leopard (average: 1.8 km and 12.3 km, respectively). The leopard’s home range (408 km2) was almost entirely within the larger home ranges of the cheetah coalition (1,137 km2). We found that the leopard approached more closely to either of the cheetahs in the rare occasions when they were separated, though whether that was the response of the cheetahs to the leopard or vice versa is unknown. This interaction eventually culminated in the leopard killing one of the cheetahs, the first documented proof of lethal competition between cheetah and leopard in Iran. The combined risks of larger home ranges beyond the protected areas with higher probability of encounters with humans, of highway crossing, and predation by Persian Leopards contribute to the particularly precarious situation of the Asiatic Cheetah.