Large Cell Ratio Research Articles

Evaluation of Hemogram Parameters in the Diagnosis of Pulmonary Embolism: Immature Granulocytes and Other New Tips.

Pulmonary embolism (PE) is an important cause of sudden death and is difficult to diagnose. Therefore unnecessary radiological investigations are often resorted to. Although some inflammatory parameters in the hemogram have been found to play a role in the diagnosis of PE, many parameters have not been adequately investigated. We aimed to evaluate potential inflammatory parameters in hemogram in the diagnosis of PE and to determine the parameters with the highest diagnostic value. This single-center, retrospective study was performed by evaluating 114 cases with suspected PE admitted to the emergency department between January 2017 and June 2022. Among 114 cases, 62 cases with a definitive diagnosis of PE by pulmonary computed tomography angiography served as the PE group and 52 cases without PE served as the control group. Admission hemogram parameters of both groups were recorded. Potential chronic diseases and acute conditions affecting hemogram were excluded from the study. In the multivariate model; immature granulocyte (IG), neutrophil/lymphocyte ratio (NLR), monocyte % and platelet large cell ratio (P-LCR) were found to be significantly and independently effective in differentiating cases with and without PE (P˂.05). Our findings suggest that high IG, high NLR, high monocyte %, and low P-LCR values have diagnostic value in cases with suspected PE. However the usability of IGs in the diagnosis of PE is a new finding. Hemogram is cheap, easily accessible, and potential inflammatory biomarkers in hemograms may increase physicians' awareness in the diagnosis of PE.

Serum VEGF, P-Selectin, HDL-C, Platelet Index, and Coagulation Function Index in Deep Vein Thrombosis after Traumatic Fracture.

The goal was to investigate the relationship between serum vascular endothelial growth factor (VEGF), P-selectin, high-density lipoprotein cholesterol (HDL-C), platelet parameters, and coagulation function indexes and postoperative deep vein thrombosis (DVT) in patients with traumatic fracture. A total of 150 patients with traumatic fractures after DVT were selected as the DVT group, and 150 patients with traumatic fractures without DVT during the same period were selected as the non-DVT group. Serum VEGF, P-selectin, HDL-C, platelet parameters including platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (PCT), platelet large cell ratio (P-LCR), and plasma coagulation function indexes including thrombin time (TT), prothrombin time (PT), activated partial thrombin time (APTT), fibrinogen (FIB), and D-dimer (D-D) were measured. Pearson's correlation was performed to analyze the correlation between serum VEGF, P-selectin, and coagulation function indexes, and binary logistic regression was used to analyze the risk factors of DVT. Serum VEGF and P-selectin in the DVT group were higher while HDL-C was lower than those in the non-DVT group (p < 0.05). Serum VEGF and P-selectin were negatively correlated with plasma D-D and FIB (p < 0.05), and serum HDL-C was negatively correlated (p < 0.05). Compared with the non-DVT group, MPV, PDW, and P-LCR in the DVT group were decreased (p < 0.05). Multivariate logistic regression analysis showed that P-LCR was a risk factor for postoperative DVT in patients with traumatic fractures (p < 0.05). Serum VEGF and P-selectin are higher and HDL-C is lower in patients with DVT after postoperative traumatic fracture than in patients without DVT. Combined detection of serum VEGF, P-selectin, HDL-C, and coagulation function indexes may help to reduce the risk of DVT. Platelet parameters (MPV, PDW, P-LCR) have certain reference values for the clinical diagnosis and disease evaluation of DVT.

Effects of methanolic curry leaf extracts (Ocimum irvinei) on the haematological parameters of Wistar rats

This study was set to evaluate the effect of administration of the methanolic extract of Ocimum Irvinei leaf on the haematological parameters of both male and female wistar rats. Previous reports have shown that this plant species is a good medicinal plant which has been used since antiquity for various ailments and also has a rich embodiment of nutritional and phytochemical constituents. Its application is mostly seen as a spice in most of dishes due to its flavouring. The haematological parameters analysed were Haemoglobin (HGB), Platelet Distribution Width (PDW), Platelet Large Cell Ratio (P-LCR), Procalcitonin Test (PCT), Haematocrit (HCT), Mean Platelet Volume (MPV), Mean Cell Volume (MCV), Mean Cell Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), Red Blood Cell Count (RBC), Red Cell Distribution Width (RDW), and White Blood Cell Count (WBC) with Mindray BC-2800 Auto Haematology Analyser. Result obtained from the study showed that there was very little difference in the haematological parameters across groups and between both sexes. This infers that administration of the leaf extract of O. irvineihas little or no pronounced effect on the blood cells. Since there was very little change observed in the results of the test subjects, it is recommended that this study should be carried out for a longer period of time (a year or more) to stand the chance of getting more pronounced results. When compared with the Rat Reference Range shown in chapter one, there were several notable differences between the results and the normal range; while some where relatively higher or lower, some were well within the range.

The day after intracerebral hemorrhage: platelet mass index as predictor of survival—a retrospective cohort study

Abstract Background Platelets are implicated in the pathophysiology of intracerebral hemorrhage (ICH). Platelet count (PLT) is affected by platelet loss, while mean platelet volume (MPV) by platelet replenishment. Whether platelet mass index (PMI), the product of PLT and MPV, might predict survival after ICH, remains unknown. Methods All first-ever ICH patients, admitted to Xanthi General Hospital between January 2018 and May 2020 and met eligibility criteria, were enrolled in this retrospective cohort study. Demographics, medical record, first-symptom-to-admission time, vital signs, modified Rankin Scale, ICH score, arterial blood gas test, complete blood count, blood biochemistry, and CT scan test were collected for each patient. PMI values on day 1 (admission; PMI1), day 2 (PMI2), and day 7 (PMI7), along with PLT, MPV, platelet distribution width (PDW), and platelet large cell ratio (P-LCR), were evaluated as potential predictors of 12-month survival using Repeated Measures General Linear Model. Binary discretization of predictors was based on optimal scaling and evaluated using binary regression. Results From 59 patients enrolled (aged 75.7 ± 12.0 years; 31 females), 29 were still alive 12 months after ICH. Age, arterial hypertension, diabetes mellitus, hemoglobin level (Hb), and oxygen saturation (O2Sat) were correlated with 12-month survival. After adjustment for these parameters, PMI1 and PMI2 were independently correlated with 12-month survival (P = 0.048 and P = 0.004, respectively), while PMI7 was not (P = 0.332). PMI2 ≥ 2,400 fL/μL was best to discriminate survivors from non-survivors (age, arterial hypertension, diabetes mellitus, Hb, and O2Sat adjusted OR 0.123 with 95% CI: 0.023–0.694; P = 0.018). Conclusions PMI within the first day after admission for ICH might be used as early predictors of survival. Properly designed prospective studies are needed to further evaluate their contribution as such.

Platelet indices and angiogenesis markers in hypertensive disorders of pregnancy.

Activated platelets exert a key role in the pathogenesis of preeclampsia (PE). There is evidence of distinctive patterns of platelet indices in PE in comparison to healthy pregnancies, therefore these indices can be potential tools for PE detection, risk stratification, and management. Considering the vascular aspects of its pathophysiology, PE is characterized by the increased levels of soluble FMS-like tyrosine kinase-1 (sFlt-1) an antiangiogenic factor, and reduced placental growth factor (PlGF), a proangiogenic factor. This study aimed to assess the platelet indices in hypertensive disorders of pregnancy (HDP) and its correlation with angiogenesis-related biomarkers. The groups for the study were: control (n = 114); gestational hypertension; (n = 112), and PE (n = 42). The platelet indices included were platelet counts (PLT-I and PLT-F), mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (PCT), platelet large cell ratio (P-LCR), and immature platelet fraction (IPF# and IPF%). Serum levels of sFlt-1 and PlGF were assessed. PLT-I, PLT-F, and PCT% were lower in PE, while MPV, PDW, P-LCR, IPF%, and IPF# were increased. The parameter MPV presented the best performance for the discrimination of PE. There was a moderate positive correlation between sFlt-1 levels and MPV, PDW, and P-LCR. Platelet indices can be potentially applied as additional tools for the diagnosis and management of HDP. Activated platelets may act as an extra source of sFlt-1 in PE.

Developing Localized Reference Intervals for Platelet Indices in South India

Introduction: The Platelet Indices (PI) are part of the most frequently done tests in the hospital. They are considered as biomarkers in numerous systemic and metabolic diseases. But the real challenge has been to define the clinical cutoffs which enable management based on the above parameters. So we tried to develop Reference Intervals(RI) for PIs to enable them for clinical use. Aim: The aim of the study was to develop RIs for the PIs from ostensibly healthy adult males from the local population. Materials and Methods: 123 healthy adult males between the age 18 to 64, were chosen after screening and their blood samples collected and examined on Sysmex XN1000 cell counter for the PIs. Results: The mean values of Platelet Count (PC), Distribution width (PDW), Mean Platelet volume(MPV), Plateletcrit (PCT), Large cell Ratio(P-LCR), Immature Platelet Fraction(IPF) and Absolute Immature Platelet Number(AIPN) were 2.58x109/L, 12.3%, 10.2fl, 0.281%, 27.17%,0.682%, and 1551.4 cells. PDW showed a strong positive correlation with MPV (r = 0.937) and P- LCR (r = 0.949). The PLT showed a very strong positive correlation with PCT (r = 0.901). The linear regression analysis of the rest of the PIs with PLT showed an 81% strong predictability for PCT. Conclusion: The sex specific, instrument specific, localized RIs developed the study was compared to other published studies World over. It was also compared to an Indian and other South Indian studies. It also offers an opportunity for institutions using this cell counter to make use of the above RIs in clinical situations.

Comparison of Thrombocyte Indices and Immature Platelet between Preeclampsia and Normal Pregnancy

The primary pathophysiology of preeclampsia is abnormal trophoblast invasion, vasospasm, and endothelial dysfunction occurring, as well as platelet activation, increased platelet consumption, and the stimulation of the release of new platelets, which affects the platelet indices and immature platelets. This study compared the platelet indices (Mean Platelet Volume=MPV, Platelets Distribution Width=PDW, Platelet Large Cell Ratio=PLCR, and Immature Platelet Fraction (IPF) between preeclampsia and normal pregnancy. This research was an unpaired comparative analytic observational study with a cross-sectional design conducted in the Tangerang District General Hospital from February to August 2020. The platelet indices (MPV, PDW, PLCR) and IPF of preeclampsia subjects (n=33) at Tangerang District General Hospital and normal pregnant subjects (n=38) at Annisa Hospital Tangerang were examined using the Sysmex XN-1000 automatic hematology analyzer. Comparative platelet indices and IPF analysis were carried out by independent T-test or Man-Whitney test. Receiver Operating Characteristics (ROC) analysis was performed to obtain the cut-off value of the platelet indices and IPF to differentiate preeclampsia and normal pregnancy. The platelet indices and IPF were significantly higher in preeclampsia. The mean (SD) MPV was 10.86 (0.9) fL and 10. (0.89) fL, p&lt;0.001; PDW 12.7 (8.7-19.1) fL and 10.85 (8.5-15.7) fL, p&lt;0.001; PLCR 31.96 (7.02) % and 25.06 (6.92) %, p&lt;0.001; and IPF 8.50 (3.10-20.40)% and 2.75 (1.2-9.4)%, p&lt;0.001, respectively in preeclampsia and normal pregnancy. ROC analysis showed that an IPF with an AUC of 0.88 (95% confidence interval (CI) 0.78-0.95) with an IPF cut-off value of &gt;6.5% was a good marker, while the MPV cut-off &gt;10.2 fL, AUC 0.75(95% CI 0.63-0.84), PDW cut-off &gt;11.3fL, AUC 0.77 (95%CI 0.65-0.86) and PLCR cut-off &gt;26.1%, AUC 0.76 (95% CI 0.65-0.85) were good enough markers to differentiate preeclampsia from normal pregnancy. Platelet indices and IPF were statistically significantly higher in preeclampsia. The IPF parameter is a good marker, while the platelet indices can be a pretty good marker to distinguish preeclampsia and normal pregnancy. The IPF parameter and platelet indices can be proposed as a routine examination in pregnancy. Changes in IPF and platelet indices can be early signs of preeclampsia or the development of preeclampsia.

Evaluation of Platelet Parameters in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma in a Background of Oral Submucous Fibrosis.

The role of platelet parameters as markers of inflammation in various diseases is now in limelight. The interaction between cancer cells and platelets is a well-established phenomenon. Oral submucous fibrosis (OSMF) is a premalignant disorder with a malignant transformation rate of 2-8%. This study aimed to evaluate platelet parameters in OSMF and oral squamous cell carcinoma (OSCC) in the background of OSMF. This cross-sectional study was performed using secondary data retrieved between January 2019 and December 2019 in the Department of Oral Pathology and the Hematology Laboratory. The data retrieved included 44 histopathologically proven OSCC in a background of OSMF (group III) and 36 OSMF (group II). The haematological parameters of these selected cases were retrieved from the Sysmex XN-1000 automated hematology analyser database. A control group (group I) comprises 50 subjects with normal (negative/unflagged) haematological parameters. All data were statistically analysed using SPSS 20.0. The significance level of tests was set at 5%. The mean platelet volume (MPV) (9.60 [±0.95] P < 0.001), platelet distribution width (PDW) (10.45 [±1.9], P < 0.001), platelet large cell ratio (PLCR) (21.70 [±7.98], P < 0.001), and the ratio of mean platelet volume to total platelet count (MPV/PLT) (0.03 [0.01], P < 0.001) were lower in group III when compared to the other two groups. Platelet parameters may be used as indices in the OSCC in the background of OSMF. However, large-scale prospective studies are necessary to evaluate the utility of these parameters during the malignant transformation of OSMF, thereby encouraging prompt treatment to prevent morbidity and mortality.

Relationship between the Consumption of Fermented Red Beetroot Juice and Levels of Perfluoroalkyl Substances in the Human Body's Fluids and Blood Parameters.

Per- and polyfluoroalkyl substances (PFASs) are a group of fluorinated, organic, man-made chemicals; they do not occur naturally in the environment. This study aimed to determine the profile and content of PFASs in the volunteers' blood plasma and urine after the consumption of fermented red beetroot juice and then correlated it with the blood parameters. Over 42 days, 24 healthy volunteers ingested 200 mL/60 kg of body weight of fermented red beetroot juice. PFASs were analyzed using the micro-HPLC-MS/MS method. Five perfluoroalkyl substances were found in the volunteers' body fluids. After consuming the juice, it was discovered that regarding the perfluorocarboxylic acids, a downward trend was observed, while regarding the perfluoroalkane sulfonates, and their plasma content showed a statistically significant upward trend. Analysis of the hematology parameters indicated that the intake of fermented red beetroot juice showed a significant decrease in mean corpuscular volume (MCV), platelets concentration, mean platelet volume (MPV), platelet large cell ratio (P-LCR) at the significance level p < 0.01, and hematocrit (p < 0.05). On the other hand, the dietary intervention also indicated a significant (p < 0.01) increase in corpuscular/cellular hemoglobin concentration (MCHC). In the case of blood biochemistry, no significant change was observed in the blood samples after the intake of the fermented beetroot juice. However, a decreasing tendency of total cholesterol and low-density lipoprotein concentration (LDL-C) was observed. Based on the presented results, there is a need to analyze and monitor health-promoting food regarding undesirable substances and their impact on consumer health.

Effect of platelet indices on postoperative pain and edema in bimaxillary surgery.

To investigate the influence of platelet volume indices (PVI) on postoperative pain and edema in patients undergoing bimaxillary surgery and assessed the associations between PVI parameters and other clinical factors. We examined the medical records of 50 patients aged 18-40, treated between 2019 and 2020. Platelet indices (platelet count [PLT]), mean platelet volume, platelet distribution width, plateletcrit (PCT), and platelet large cell ratio) were analyzed. Postoperative pain and edema were assessed based on the frequency of intravenous (IV) analgesic administration and 3D imaging. A lasso-penalized regression was used for the analysis. Significant positive correlations were observed between PLT and postoperative edema on the first (T1-0) and third (T3-0) postoperative days. Furthermore, PLT was positively associated with the number of IV analgesic drug administrations from 24 to 72 hours after surgery. Additionally, a discernible positive correlation was identified between PCT levels and the quantity of IV analgesic drugs administered within the first 24 hours after surgery. Platelet indices, particularly PLT and PCT levels, were associated with postoperative pain and edema in patients undergoing bimaxillary surgery. These indices have the potential to serve as biomarkers for predicting and managing postoperative complications of orthognathic surgery. Further studies are required to explore the clinical utility and implications of these findings.

Effect of Platelet Parameters on Linezolid-Related Thrombocytopenia in Hospitalized Patients.

Linezolid-induced thrombocytopenia incidence varies considerably. Linezolid-related thrombocytopenia in patients has received few studies which have investigated risk factors including platelet parameters except for platelet counts. The study aims to analyze the effect of platelet parameters, including mean platelet volume and platelet large cell ratio, on linezolid-related thrombocytopenia in patients. The effect of platelet parameters on linezolid-related thrombocytopenia was identified by univariate and multivariate logistic regressions. A Kaplan-Meier survival analysis was carried out to compare the survival of patients who developed linezolid-related thrombocytopenia with patients who did not. Thrombocytopenia occurred at a rate of 41.5% (66/159) after linezolid therapy in hospitalized patients. Platelet parameters, including the difference in mean platelet volume (MPV/fL=0.08 (-1.2-0.9)vs-0.5 (-1.5-0.3), (OR, 0.459; P = 0.001), the difference in platelet large cell ratio (PLCR/fL=0.9 (-5.1-6.2)vs-3.8 (-8.6-2.4), (OR, 1.156; P = 0.001), baseline platelet counts (OR, 0.995; P = 0.006) and duration of linezolid therapy≥10d (OR, 1.346; P = 0.007), were significantly associated with linezolid-related thrombocytopenia in hospitalized patients. In addition, other risk factors which also are associated with linezolid-related thrombocytopenia include baseline red blood cells, co-medication with parecoxib and co-medication with caspofungin. Accumulated in-hospital mortality of patients with thrombocytopenia was significantly higher than that of patients without thrombocytopenia during linezolid treatment (19.7% vs 8.6%, P = 0.003). The difference in mean platelet volume, the difference in large platelet ratio, baseline platelet counts and duration of linezolid therapy≥10d significantly affected the development of linezolid-related thrombocytopenia in hospitalized patients.

The effects of type of recovery in resistance exercise on responses of platelet indices and hemodynamic variables.

To examine the effects of two different volume-matched resistance exercise (RE) recovery protocols (passive and active) on platelet indices and hemodynamic variables. Twelve Healthy participants (mean ± SD; 25 ± 3 yrs) completed a traditional resistance exercise (TRE) protocol that included three sets of six repetitions at 80% one repetition maximum (1RM) with two minutes passive recovery between sets, exercises and an interval resistance exercise (IRE) protocol that included three sets of six repetitions at 60%1RM followed by active recovery including six repetitions of the same exercise at 20%1RM. Blood samples for multiple platelet indices were taken before the protocols, immediately-post (IP), and after 1-hour recovery. Hemodynamic variables were measured before, IP, and every five minutes during recovery. Mean platelet volume and platelet large cell ratio P_LCR decreased from baseline to recovery. Heart rate (HR) and rate pressure product (RPP) were augmented at IP following IRE compared to TRE. HR was significantly elevated for 20 minutes after both RE protocols, and RPP recovered by five minutes. Systolic blood pressure was increased at IP compared to baseline and all recovery time points for both RE protocols. Our research demonstrated that both RE protocols, produced transient increases in platelet indices (MPV, and P_LCR) and hemodynamic variables (SBP, HR, and RPP), all of which returned to baseline within an hour. Notably, the IRE protocol elicited a greater increase in HR and RPP compared to the TRE protocol.

Relationship of platelet subgroups with prognosis and mortality in patients with mild, severe and critical COVID-19

Introduction: This study investigates whether platelet-related indicators including platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), Plateletcrit (PCT), and platelet large cell ratio (P-LCR) could be effective biomarkers in the prognostic of 2019-novel coronavirus disease (COVID-19) fatality. &#x0D; Methods: Out of 380 patients diagnosed with COVID-19, 98 patients were randomly and retrospectively were included in our study. Detection of COVID-19 was performed according to the World Health Organization (WHO) guidelines and using quantitative polymer chain reaction (qPCR). Clinical and laboratory data and medical records for all study participants were collected from a digital version of their health history at Ayancik State Hospital and Diyarbakır Gazi Yaşargil Training and Research Hospital. All patients were followed up daily until discharge or death and were eventually classified into three categories: Mild, severe, and critical patients. Data analysis was performed using SPSS-26 for Windows (Statistical Package for Social Science, SPSS Inc. Chicago IL, USA®Z), and a 95% confidence level was selected. P &lt; 0.5 was considered statistically significant.&#x0D; Results: Of participants, 64.3% of the patients are 65 years and older, 54.1% are male, 60.2% survived, 39.8 % died and 35.7% are in critical condition. The mortality rate is higher in males and 65 years and older. While PLT (190,24) and PCT (0,21) measurements were higher in surviving patients, MPV (10,67), PDW (16,15) and P-LCR (30,82) measurements were higher in deceased patients. PDW and PCT measurements are more effective in predicting mortality state according to Roc analysis. The measurement that best predicts the deceased status of patients who are positive according to the cut-off value determined is PDW (0.621), and the measurement that best estimates the surviving patients is PLT (0.925).&#x0D; Conclusions: In our study, it was concluded that high MPV, PDW and P-LCR and low platelet values are associated with poor prognosis of COVID-19 disease, and PDW and PCT measurements are more effective in predicting mortality in patients with COVID-19 infection. Laboratory parameters such as MPV, PDW, P-LCR, PDW, and PCT can provide clinicians with predictions of prognosis and mortality from COVID-19 disease.

Platelet Indices as a Tool for Differentiation between Clonal Thrombocytosis and Reactive Thrombocytosis

Objective: To determine the efficacy of platelet indices as a tool for differentiation between clonal thrombocytosis and reactive thrombocytosis. Study Design: Cross sectional study. Place and Duration of Study: Department of Pathology, Combined Military Hospital, Lahore Pakistan, from Nov 2019 to Oct 2020. Methodology: Fifty-six subjects were included in Reactive thrombocytosis group and 56 in Clonal thrombocytosis group. Fresh blood in EDTA anticoagulant was analyzed to determine complete blood counts and platelet parameters (Platelet distribution width, mean platelet volume, Platelet large cell ratio, Plateletcrit) using the automated hematological analyzer Sysmex KX-21. Results: Mean age of the patients was 45.76 years with a range of 17-75 years. Assessing the cause of reactive thrombocytosi revealed that 20(35.7%) was infection and 16(28.5%) was iron deficiency anemia. In clonal thrombocytosis group 21(37.5%) were diagnosed with Chronic myeloid leukemia, 13(23.2%) with Essential thrombocythemia and 14(25%) with Polycythemia rubera vera. Platelet indices were compared in clonal thrombocytosis group and the reactive thrombocytosis group. Mean platelet volume, Platelet large cell ratio and Plateletcrit were significantly higher in the clonal thrombocytosis group (p=0.001). Difference in Platelet distribution width was not found to be statistically significant in the two groups (p=0.07) Conclusion: Platelet indices were higher in the clonal thrombocytosis group. Along with platelet count, they can serve as an efficient and cost-effective method in differentiating between clonal and reactive thrombocytosis.

Safety and feasibility of platelet transfusion through long catheters in the neonatal intensive care unit: an in vitro study

ObjectiveTo assess the safety and feasibility of platelet transfusion through small-bore long lines used in the neonatal intensive care unit (NICU), including double-lumen umbilical venous catheters (UVCs) and 24 G...

Full blood count and some haemorheologic variables of vendors exposed to liquefied petroleum (cooking) gas: a comparative study

Introduction: Cooking gas in the form of liquefied petroleum gas is sold in gas stations and outlets by vendors who do not use personal protective equipment despite working in a high-risk environment hence they have a high exposure to inhalation of the chemicals in the gas. This study assessed the full blood count and haemorheologic variables of vendors exposed to liquefied petroleum gas. Methods: Forty gas vendors were enrolled from four gas stations in Calabar while forty apparently healthy individuals who do not work in gas-related jobs were recruited as control. Ethical approval was granted by the Cross River State Ministry of Health, Calabar. Permission to enroll workers was obtained from the management of gas stations while study subjects gave informed consent. A structured questionnaire was used to obtain demographic information and health history while blood pressure, weight and height were measured using standard instruments; the body mass index was calculated. The full blood count and haemorheologic variables were determined by standard methods. Data analysis was done using a student t-test on SPSS version 21 and a P value ≤0.05 was considered significant. Results: The mean age was 25.9±3.2 years and 26.5±6.5 years for the test and control groups with gas vendors being predominantly males (97.5%). Work duration of 97.5% of gas vendors was ≤5 years. Sixty percent of the control and 42.5% of the gas vendors engaged in regular exercise while 40% and 57.5% do not. Sixty-seven-point five percent (67.5%) of the control rarely go for medical checks and 27.5% had never had a medical check with only 2% who check often. For the gas vendors, 55% have never had a medical check while 45% of them rarely check. Also, 42.5% of the control have never or rarely checked their blood pressure while 15% check often; 62.5% of the gas vendors have never checked their blood pressure while 37.5% rarely check. There was a family history of hypertension for 2 (5%) of the control and none for the test group. Also, 1 (2.5%) of the gas vendors had a history of fainting episodes while 39 of them and the control did not. Only 10% and 20% of the gas vendors use nose mask and hand gloves with the use of overalls and safety boots being 40% for each. The body mass index of the control group (25.15kg/m2) was not different (P&gt;0.05) from that of the test group (24.53kg/m2). Whereas the systolic blood pressure was higher for the gas vendors than the control (126.47mmHg and 120.32mmHg), the diastolic blood pressures were comparable (74.60mmHg versus 72.92mmHg). The full blood count shows the red blood cell count, haematocrit and haemoglobin of the gas vendors were significantly higher (P&lt;0.05) than the control value while the mean corpuscular volume and mean corpuscular haemoglobin were comparable (P&gt;0.05). Again, the mean corpuscular haemoglobin concentration and red cell distribution width standard deviation of the gas vendors were significantly higher (P&lt;0.05) versus the control while the red cell distribution width coefficient of variation was comparable (P&gt;0.05). The total white blood cell count and the absolute granulocyte and lymphocyte counts of the gas vendors were significantly higher (P&lt;0.05) than the control value while the mixed leucocyte count was not different (P&gt;0.05) between the two groups. The platelet count, mean platelet volume, platelet distribution width, plateletcrit, platelet large cell ratio and platelet large cell count were significantly higher (P&lt;0.05) for the gas vendors when compared to the control. Relative plasma viscosity and fibrinogen concentration were significantly higher (P=0.030) when the test group was compared to the control. Conclusions: This study has demonstrated an increase in red cell, white cell and platelet parameters of vendors exposed to cooking gas as a probable response to an induced state of chronic inflammation. There is also impaired haemorheology as expressed by an increase in relative plasma viscosity and fibrinogen concentration with possible pre-disposition to cardiovascular disease as a consequence. Management of cooking gas stations and the vendors should be educated on the need to use personal protective equipment in order to reduce exposure to the components of cooking gas.

Red cell distribution width, platelet distribution width, and plateletcrit as indicators of prognosis in COVID-19 patients - A single-center study

Background: COVID-19 is still present in the world, though the extent varies by region and country. According to the World Health Organization, there have been over 617 million confirmed cases of COVID-19 and over 13 million deaths worldwide since the pandemic began on March 10, 2023. Aims and Objectives: This is a study conducted with the aim of providing biomarkers to predict COVID-19 disease progression and mortality based on red cell indices and platelet indices which are commonly measured as part of a complete blood count (CBC). Materials and Methods: A prospective study was conducted during the peak of the second wave of COVID-19 from March 2021 to June 2021. The study included 540 patients who were admitted to the Government General Hospital, Nizamabad, and had tested positive for COVID-19 by RT-PCR. Red Blood Cell (RBC), Hematocrit (HCT), Red cell indices like Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), Red Cell Distribution width (RDW) and Platelet indices like Mean Platelet Volume (MPV), Platelet Distribution Width (PDW), Plateletcrit (PCT), Platelet–Large Cell Ratio were taken from CBC analyzer Sysmex XN-1000 and analyzed statistically. The patients were then followed up for a period of 14 days to track their outcomes. Results: In the data, majority were male n=334 (62%) and n=280 (38%) were female. 70.37% (n=380) were survivors and 29.63% (n=160) were non-survivors. Red blood cell, red cell indices such as RDW-CV and RDW-SD, and platelet indices such as PCT and PDW were significantly higher in non-survivors compared to survivors with P&lt;0.05. Conclusion: Non-survivors had significantly higher levels of RDW-CV, RDW-SD, PCT, and PDW compared to survivors. These parameters in combination can be useful for predicting COVID-19 mortality at early stage in forthcoming waves.

Platelet indices as a predictor of microvascular complications of type 2 diabetes mellitus

Abstract Background: The burden of diabetes is increasing day by day, and diabetic individuals are predisposed to develop micro- and macrovascular complications leading to increased mortality and morbidity. Platelet indexes can alert us regarding endothelial dysfunction in diabetes and in turn regarding microvascular complications. Materials and Methods: A case–control study was conducted on 100 diabetic patients with age and sex matched and 100 nondiabetic controls. All diabetic patients were evaluated for microvascular complications such as diabetic neuropathy, retinopathy, and nephropathy. Clinical history of all patients was taken regarding associated comorbidities and association. Fasting and postprandial blood sugar, HbA1c, platelet count, and platelet indices were measured. Statistical analysis was performed by using student unpaired t test and χ2 test. Results: Platelet indices mean platelet volume, plateletcrit, platelet distribution width (PDW), and platelet large cell ratio (P-LCR) were significantly higher in diabetic patients compared with controls. It was also observed that changes in mean platelet volume, PDW, and P-LCR were more significant in diabetic patients with microvascular complications than diabetic patients without microvascular complications. Conclusion: Values of platelet indices were statistically associated with diabetes and its microvascular complications.

Evaluate the Complete Blood Picture and Some Biochemical Parameters in Patients referred to the dialysis unit in Diyala General Hospital

Research done on 40 patients; 24 males, 20-79 years, and 16 females, 42-67 years; referred to dialysis unit, Baqubah hospital, in November and December, 2021; January and February 2022. Creatinine, Urea and Iron, record high levels. Glucose, calcium, sodium, potassium, and phosphate, were within normal, and low levels of albumin. Means of Total Erythrocyte Counts (TECs), hemoglobin (Hb), Hematocrit% (HCT), and Mean Corpuscular Hemoglobin Concentration (MCHC), showed lowered level. While, Mean Cell Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Red Blood Cell Distribution Width- Standard Deviation (RDW-SD), Mean Red Blood Cell Distribution Width- Coefficient of Variation (RDW-CV), were within normal. Total Leucocyte Counts (TLCs), Neutrophils, Lymphocytes, Eosinophil, Basophils, were within normal, Platelet Counts and related parameter were within normal. Within ranges some values were higher than normal as, potassium, phosphate, (RDW-SD), (RDW-CV), Neutrophils%, Eosinophil%, Lymphocytes, Mean Platelets Volume (MPV). While some other values were lower as sodium, (TRCs), (Hb), (HCT %), (MCV), (MCH), (MCHC), (RDW-SD), (TLCs), Lymphocytes%, platelets, Platelet Distribution Width (PDW), Platelet- Large Cell Ratio (P-LCR). But some values of glucose, and calcium, were high, and some other low. At the same time Monocytes%, Basophils% was within normal range. In conclusion creatinine and urea levels have an important role in diagnosis and follow- up of kidney failure. All patients have high levels of urea, iron and creatinine. But not all have higher level of potassium and glucose. Sodium and albumin, showed decreased level. Blood picture revealed microcytic, hypochromic anemia; as (TECs), (Hb), (HCT %), (MCHC) and (RDW-SD) were of lower values.

Comparison of platelet indices in dengue fever patients based on platelet transfusion: A prospective observational study in a tertiary care center.

Dengue fever is the most prevalent mosquito-borne viral disease in humans. Platelet indices (PIs) are given by the cell counters but are often not reported which is possibly due to under-recognition of the utility of these parameters. This study aimed to compare PIs in patients with dengue fever to assess their role in the outcome such as hospital stay and platelet transfusion requirements. Prospective observational study in a tertiary care center, Thrissur, Kerala. A group of 250 dengue patients was studied over a period of 18 months. The platelet parameters (platelet count, mean platelet volume [MPV], platelet distribution width [PDW], platelet large cell ratio [PLCR], plateletcrit [PCT] and immature platelet fraction [IPF]) were measured with Sysmex XN-1000 and followed up every 24 h. The clinical features, duration of hospital stay and platelet transfusion requirements details were collected. Independent t-test, Chi-square test, Karl Pearson correlation coefficient. A total of sample size was 250. The study showed normal PDW and MPV, low platelet count and PCT, and high PLCR and IPF in dengue patients. There were significant differences in PIs (lower platelet count and PCT, higher MPV, PDW, PLCR, and IPF) in comparison between dengue patients based on platelet transfusion. PIs may act as a predictive tool in the diagnosis and predicting outcomes in dengue fever. Low platelet count and PCT, high PDW, MPV, PLCR, and IPF in transfused dengue patients were found to be statistically significant. Clinicians need to be sensitized about the utility and limitations of these indices and rationalize the need for red cell and platelet transfusions in dengue.