The hydrological characteristics of a watershed play a crucial role in shaping ecosystems within the Boreal zone and have a significant impact on regional environments. Knowing these characteristics, such as the distinctive topography, vegetation, soil composition, and climatic conditions in the Canadian Boreal ecozone, is essential for implementing sustainable water management. This study focuses on assessing the hydrological dynamics of the Upper Humber River Watershed (UHRW) in western Newfoundland, Canada, using the Soil and Water Assessment Tool (SWAT) model. The UHRW includes sub-basins and hydrological response units (HRUs), with diverse land uses, soil types, and slope characteristics. Key parameters influencing streamflow simulation were identified through sensitivity analysis, including the runoff curve number, the effective hydraulic conductivity, the temperature lapse rate, the soil evaporation compensation factor, and the available water capacity of the soil layer. The SWAT model, using data from the Reidville hydrometric station, shows favorable performance metrics, with R2 values of 0.79 and 0.83 during the calibration and evaluation periods, respectively. The model effectively captures seasonal and monthly flow patterns, displaying right-skewed distributions and seasonal variations. The analyzed hydrological parameters, such as precipitation, evaporation, transpiration, surface runoff, and groundwater flow, reveal their significant contributions to the water balance. The flow duration curve analysis indicates the model’s capability to estimate peak and low flows, with slight under-prediction during the recession phase. Seasonal analysis further supports the model’s performance, with positive Nash-Sutcliffe Efficiency (NSE) values ranging from 0.65 to 0.91. The study concludes that the SWAT model is suitable for simulating the hydrological processes in the studied watershed providing valuable insights for sustainable water resource management and decision-making in the UHRW. The results can be useful for other Boreal ecozone watersheds.