Laparoscopic intracorporeal knot tying is a difficult skill to acquire. Currently, time to complete a knot is the most commonly used metric to assess the acquisition of this skill; however, without a measure of knot quality, time is a poor indicator of skills mastery. Others have shown that knot quality can be accurately assessed with a tensiometer, but obtaining this type of assessment has typically been cumbersome. We investigated a new method of real-time assessment of knot quality that allows for more practical use of knot quality as a performance metric. Eleven experienced endoscopic surgeons tied 100 intracorporeal knots in a standard box trainer. Each of the knots was immediately tested using the InSpec 2200 benchtop tensiometer (INSTRON, Canton MA) where a knot quality score (KQS) is generated based on the load handling properties of the knotted suture. The execution time was also recorded for each knot. The assessment of all knots ended with one of two end points: knots that slipped (n=48) or knots that held until the suture broke (n=52). Knots that slip are generally of poorer quality than those that held. Execution time did not correlate with knot-quality score (r=0.009, P=.9), and the mean execution time did not differ significantly between slipped and held knots (65 vs 68 seconds, P=.8). No completion time criteria were able to accurately predict slipped versus held knots. The mean KQS difference between held and slipped knots was highly significant (24 vs 12, P<.0001). A knot with a KQS exceeding 20 was nearly 10 times more likely to hold than slip. Time to complete a knot is a poor metric for the objective assessment of intracorporeal knot-tying performance in the absence of a measure of knot quality. Real-time evaluation of the knot quality can accurately distinguish well-tied knots from poorly tied knots. This mode of assessment should be incorporated into training curriculum for surgical knot tying.