Landscape Modification Research Articles

Integrating historical and recent data to measure long-term trends of endangered subterranean species

Documenting population trends is pivotal to identify the underlying drivers of biodiversity changes and setting conservation priorities. Ascertaining trends often requires long-term, standardized, monitoring data that are not always available. Historical data provide important information on past species distribution, but their integration with recent data to obtain trend estimates is challenging. Here we show how site occupancy-detection models (SODMs) can allow combining data from recent monitoring with historical ones from the gray literature. Using data on the endangered cave salamander, Speleomantes strinatii, we tested whether SODMs can provide reliable trend estimates if i) historical data include repeated within-season surveys enabling the estimation of past detectability, or if ii) information on detection/non-detection is not available. We conducted repeated surveys across 40 caves covering the species range, for which historical (1940–1982) biospeleological data were available. We then developed Bayesian SODMs i) estimating species detectability from both recent and past surveys, and then assessing trends; ii) in absence of estimates of past detectability, assessing trends by comparing scenarios on the potential misdetection rate during historical surveys. Salamanders were widespread in the study sites. SODM estimated high detectability for both recent and historical surveys and suggested a growing occupancy. Changes in occupancy were unrelated to landscape modifications. Even without historical detection/non-detection data, realistic scenarios of past misdetection consistently suggested an increasing or stable trend. The application of tailored analytical approaches is fundamental to exploit the vault of information available in historical data, and can be linked to adaptive management to promote efficient conservation actions.

Urban oasis? Abundant dice snake (Natrix tessellata) populations along artificial lakeside habitats in urban landscapes

ContextHuman-induced landscape modification, such as urbanization, creates new environments that can have adverse effects on flora and fauna, posing threats to biodiversity. Understanding how reptiles respond to urbanization is crucial, especially in light of their ongoing population declines.ObjectivesWe examined the influence of landscape-scale and local-scale urbanization features on the abundance of an aquatic snake species. Our investigation focused on dice snakes (Natrix tessellata) inhabiting a lake with a heavily urbanized shoreline.MethodsWe conducted visual encounter surveys at 25 study sites during the activity period of dice snakes around Lake Balaton in Hungary. We measured both landscape-scale and local-scale variables, including urban land use cover, vegetation cover, road cover, distance of main roads and city size, emergent vegetation cover and the area of artificial rock and concrete shoreline protection structures. We analysed snake survey data using N-mixture models to estimate abundance and examine relationships with landscape-scale and local-scale variables.ResultsUrban land use cover, road cover, the proximity of main roads and the extent of artificial rock and concrete shoreline protection structures positively affected the abundance of snakes. These findings imply that urban habitats may offer new ecological opportunities for dice snakes.ConclusionsThe findings of this study indicate that both landscape-scale and local-scale human-induced landscape modifications may have a positive impact on the abundance of urban snakes. Taken together, our findings suggest that urbanization is a complex phenomenon, affecting species at different levels and with subtle effects.

DIPG-45. TARGETING HYPERACETYLATION IN PEDIATRIC DIFFUSE MIDLINE GLIOMAS USING ONCOLYTIC ADENOVIRUSES

Abstract BACKGROUND H3K27-altered pediatric diffuse midline glioma (DMG) is a highly aggressive type of brain tumor with a 5-year survival rate of less than 2%. Conventional treatments are restricted to palliative radiation therapy. Therefore, effective treatments are urgently needed. Results from a recent phase I clinical trial for Diffuse Intrinsic Pontine Glioma showed that intratumoral administration of Delta-24-RGD oncolytic adenovirus in combination with radiation improves survival without unwanted toxicity. Based on the rationale that, during infection, the early viral protein E1A binds P300, we hypothesize that combining oncolytic adenoviruses with p300 inhibitors will induce a robust modification of the post-translational landscape of DMGs and increase the anti-cancer effect of the oncolytic virus. METHODS Studies on Delta-24-RGD infectivity and replication were performed in a panel of clinically significant DMG cells. Modification of the acetylation levels of acid-extracted histones after Delta-24-RGD was analyzed using Western blot. Cell Titer Blue was utilized to measure cell viability, and ZIP-synergy scores were calculated using SynergyFinder. RESULTS We observed a time-dependent reduction in H3K27ac in human DMG cells following Delta-24-RGD infection with an 89% reduction in H3K27ac at 48h post-infection. Delta-24-RGD treatment of DMG cells resulted in optimum viral infectivity and replication. Combining Delta-24-RGD with the p300 inhibitor C646 resulted in enhanced cell death in vitro. Using a panel of oncolytic viruses that induce differential enzymatic and binding functions of P300, we showed that Delta-24-RGD mediated alteration of P300 activity is required for hypoacetylation in DMGs. We characterized the in vivo dynamics of K27M-H3.1 and K27M-H3.3 DMG-derived tumors in the brain stem of syngeneic animal models. In vivo experiments are in progress to examine the combinatorial anti-glioma effect of Delta-24-RGD and P300 inhibitors. CONCLUSION Collectively, our studies provide a strong mechanistic rationale for combining oncolytic adenoviruses and p300 inhibitors and should propel the eventual clinical testing of this approach in DMG patients.

Strigolactone induces D14-dependent large-scale changes in gene expression requiring SWI/SNF chromatin remodellers.

Strigolactones (SL) function as plant hormones in control of multiple aspects of plant development, mostly via the regulation of gene expression. Immediate early-gene regulation by SL remains unexplored due to difficulty in dissecting early from late gene expression responses to SL. We used synthetic SL, rac-GR24 treatment of protoplasts and RNA-seq to explore early SL-induced changes in gene expression over time (5-180 minutes) and discovered rapid, dynamic and SL receptor D14-dependent regulation of gene expression in response to rac-GR24. Importantly, we discovered a significant dependence of SL signalling on chromatin remodelling processes, as the induction of a key SL-induced transcription factor BRANCHED1 requires the SWI/SNF chromatin remodelling ATPase SPLAYED (SYD) and leads to upregulation of a homologue SWI/SNF ATPase BRAHMA. ATAC-seq profiling of genome-wide changes in chromatin accessibility in response to rac-GR24 identified large-scale changes, with over 1400 differentially accessible regions. These changes in chromatin accessibility often precede transcriptional changes and are likely to harbour SL cis-regulatory elements. Importantly, we discovered that this early and extensive modification of the chromatin landscape also requires SYD. This study, therefore, provides evidence that SL signalling requires regulation of chromatin accessibility, and it identifies genomic locations harbouring likely SL cis-regulatory sequences.

Drainage capture driving landscape rejuvenation in a tectonic quiescent setting: A case of study in the southern sector of the Sierra Madre Oriental (Eastern Mexico)

Drainage captures increase the stream discharge and the potential ability of rivers to incise. In quiescent tectonic settings, drainage captures are rapid geomorphic processes that can trigger landscape rejuvenation. We study a drainage capture that renewed the incision of the Moctezuma River, which flows along a mountainous flank of the southern sector of the Sierra Madre Oriental (SMO). The subsequent baselevel drop along the Moctezuma River steepened its adjacent tributaries and generated the migration upstream of knickpoints. We elucidated the resultant pattern of landscape rejuvenation along the Moctezuma River by performing different hillslope and channel metrics. We also identified and analyzed the knickpoints to confirm their origin by one incision pulse migrating upstream from the Moctezuma River. Using the stream power-based celerity model on knickpoints, we estimated that the landscape rejuvenation due to drainage capture initiated ∼0.27 Ma ago with a migration rate of ∼1 mm yr −1. Our results confirm that drainage capture is an important mechanism that produces meaningful and complex landscape modifications likewise those produced by the tectonic activity.

Is Bergen Unseasonal? On Europe’s Shifting Relation to Seasons

This article reflects on whether and how European communities’ cultural frameworks of seasons are coming to poorly correspond to the climatic conditions they experience, and the implications for how Europe adapts to climatic (and social and environmental) change. It starts from a colder- and drier-than-normal autumn and winter (2023/2024) in Bergen, Norway, and a local researcher’s investigation into why these climatically anomalous seasons were being culturally celebrated as ‘seasonal weather’. He compares studies into the Bergen population’s cultural expectations for weather conditions in each of the four seasons, with the statistical climatic record, and reveals a mismatch. He argues that the four-season framework prominent in Europe poorly describes or anticipates meteorology in Bergen, and that other frameworks could fit better. The article argues that seasonal frameworks continuously evolve with interlinked environmental and social change – from drivers such as climate change, landscape modification, social evolution, and globalization – so that seasonal mismatches are as much about how societies culturally re-conceive of seasons as about physical climate change for instance. This is important because the way European societies divide the year by seasonal expectations affects how they relate to the meteorological conditions they come to face each season.

Urban Environmental Planning in Shimla Smart City: Review of Related Literature

Urban environmental concerns are on rise due to increasing population pressure on finite natural resources, deforestation, over grazing, unplanned construction, networking of roads and large-scale modification of natural landscape. It has caused irreversible damage to the ecology of this Himalayan state. Number of ultra modern projects, road networking, hydropower projects, and SEZ and real estate projects are costing Himalayan ecology. Unplanned developmental activities and urban processes in city have resulted in several ecological problems like environmental pollution, noise pollution extreme climatic conditions, shortage of drinking water, landslides, sinking land and change in seasonal cycle. Poor urban planning will cause social, economic and environmental implications in long term. Cities needs to be planned professionally keeping in mind future sustainability. Before planning a city in hilly areas, needs proper understanding of complex and dynamic phenomena involving interaction between environmental and socio- economic and political factors with multi disciplinary approach and vision. Urbanization and industrialization on one side is contributing its share in economic development of a region but on the other hand, it is degenerating water, air, soil and natural resources resulting in the degradation of environmental quality (Anjum, 1998). Urban environmental degradation leaves direct impact on the ecology and causes ecological imbalance (Singh, 1996). The most striking reason for the environmental degradation is the changing man and environment relationship. (Dasman, 1976), Shah (2001). Developmental activities in Himalayan region need different treatment and systematic planning.

Urban boundary‐layer flows in complex terrain: Dynamic interactions during a hot and dry summer season in Phoenix, Arizona

AbstractAnthropogenic modification of natural landscapes to urban environments impacts land–atmosphere interactions in the boundary layer. Ample research has demonstrated the effect of such landscape transitions on development of the urban heat island (UHI), but considerably less attention has been given to impacts on regional wind flow. Here, we use a set of high‐resolution (1 km grid spacing) regional climate modeling simulations with the Weather Research and Forecasting model coupled to a multilayer urban canopy scheme to investigate the dynamical interaction between the urban boundary layer of the Phoenix metro (United States) area and the thermal circulation of the complex terrain it resides within. We conduct paired simulations for the extremely hot and dry summer of 2020, using a contemporary urban representation and a pre‐settlement landscape representation to examine the effect of the built environment on local to regional‐scale wind flow. Analysis of our simulation results shows that, during the summer of 2020, (a) the thermo‐topographical circulation dominates over both urban and rural areas for a majority of the diurnal cycle; (b) the built environment obstructs wind flow in the inertial sublayer during the late afternoon and the nighttime, whereas more intense daytime urban sensible heat flux dampens the urban‐roughness‐induced drag effect through a deeper urban boundary layer and vigorous mixing; (c) the Phoenix metro UHI does not result in a well‐developed and clearly discernible induced circulation as observed in other urban areas and described in the scientific literature; (d) shortly before dawn, the local UHI is able to affect the local thermo‐topographical circulation through flow intensity modulation that results in an ~10 km eastward shift of the center of mass convergence. Our results highlight the need for future research—both observational and simulation based—into urbanizing regions where multiscale flows are dominant.

A First Look at the Relationship Between Large Herbivore-Induced Landscape Modifications and Ixodes ricinus Tick Abundance in Rewilding Sites.

Background: While the influence of landscape and microclimatic conditions on tick populations is well-documented, there remains a gap in more specific data regarding their relationship to rewilding efforts with large herbivore activity. Objective: This pilot study, spanning from 2019 to 2021, explores the effects of naturalistic grazing by large semi-wild ungulates on tick abundance in the Milovice Reserve, Czechia. Methods: Tick collection was observed using flagging techniques at two distinct sites of rewilding area: one grazed, actively utilized by animals involved in the rewilding project, and one ungrazed, left fallow in neighboring areas utilized only by wild animals. Transects, each measuring 150 m in length and 5 m in width (750 m2), were established at these two sampling locations from March to September between 2019 and 2021. To minimize potential bias resulting from tick movement, a 300 m buffer zone separated the two sites. Data analysis employed a generalized estimating equations (GEE) model with negative binomial regression. The study assessed potential variations in tick abundance between selected transects, considering factors such as plant cover seasonality, temperature, and humidity. Results: During the collection periods, we gathered 586 live ticks, with 20% found in grazed areas and 80% in ungrazed areas. Notably, tick abundance was significantly higher in ungrazed areas. Peaks in tick abundance occurred in both grazed and ungrazed areas during spring, particularly in April. However, tick numbers declined more rapidly in grazed areas. Microclimatic variables like temperature and humidity did not significantly impact tick abundance compared to landscape management and seasonal factors. Conclusion: Rewilding efforts, particularly natural grazing by large ungulates, influence tick abundance and distribution. This study provides empirical data on tick ecology in rewilded areas, highlighting the importance of landscape management and environmental factors in tick management and conservation. Trophic rewilding plays a crucial role in shaping ecosystems and tick population dynamics in transformed landscapes.

Effective dispersal patterns in prairie plant species across human-modified landscapes.

Effective dispersal among plant populations is dependent on vector behaviour, landscape features and availability of adequate habitats. To capture landscape feature effects on dispersal, studies must be conducted at scales reflecting single-generation dispersal events (mesoscale). Many studies are conducted at large scales where genetic differentiation is due to dispersal occurring over multiple generations, making it difficult to interpret the effects of specific landscape features on vector behaviour. Genetic structure at the mesoscale may be determined by ecological and evolutionary processes, such as the consequences of vector behaviour on patterns of gene flow. We used chloroplast haplotypes and nuclear genome SNP surveys to identify landscape features influencing seed and pollen dispersal at a mesoscale within the Rogue River Valley in southern Oregon. We evaluated biotic and abiotic vector behaviour by contrasting two annual species with differing dispersal mechanisms; Achyrachaena mollis (Asteraceae) is a self-pollinating and anemochoric species, and Plectritis congesta (Caprifoliaceae) is biotically pollinated with barochoric seeds. Using landscape genetics methods, we identified features of the study region that conduct or restrict dispersal. We found chloroplast haplotypes were indicative of historic patterns of gene flow prior to human modification of landscapes. Seed dispersal of A. mollis was best supported by models of isolation by distance, while seed-driven gene flow of P. congesta was determined by the distribution of preserved natural spaces and quality habitat. Nuclear genetic structure was driven by both pollen and seed dispersal, and both species responded to contemporary landscape changes, such as urban and agricultural conversion, and habitat availability.

Marine introgressions and Andean uplift have driven diversification in neotropical Monkey tree frogs (Anura, Phyllomedusinae).

The species richness in the Neotropics has been linked to environmental heterogeneity and a complex geological history. We evaluated which biogeographic processes were associated with the diversification of Monkey tree frogs, an endemic clade from the Neotropics. We tested two competing hypotheses: the diversification of Phyllomedusinae occurred either in a "south-north" or a "north-south" direction in the Neotropics. We also hypothesized that marine introgressions and Andean uplift had a crucial role in promoting their diversification. We used 13 molecular markers in a Bayesian analysis to infer phylogenetic relationships among 57 species of Phyllomedusinae and to estimate their divergence times. We estimated ancestral ranges based on 12 biogeographic units considering the landscape modifications of the Neotropical region. We found that the Phyllomedusinae hypothetical ancestor range was probably widespread throughout South America, from Western Amazon to Southern Atlantic Forest, at 29.5 Mya. The Phyllomedusines' ancestor must have initially diverged through vicariance, generally followed by jump-dispersals and sympatric speciation. Dispersal among areas occurred mostly from Western Amazonia towards Northern Andes and the South American diagonal of dry landscapes, a divergent pattern from both "south-north" and "north-south" diversification hypotheses. Our results revealed a complex diversification process of Monkey tree frogs, occurring simultaneously with the orogeny of Northern Andes and the South American marine introgressions in the last 30 million years.

Geomorphic changes after the 2021 Central European flood in the Ahr Valley by LiDAR-based differences

BackgroundIn July 2021, destructive floods in Western Europe were triggered by enormous precipitation rates related to a low-pressure system named "Bernd." These catastrophic events led not only to major damage to infrastructure, severe economic losses, and the loss of lives but also to significant landscape changes and modifications. Here, we focus, as a case study, on the flood aftermath of the Ahr Valley in Rhineland-Palatinate state in western Germany, as it was one of the most affected and destroyed regions by the flood. We utilize high-resolution Digital Terrain Models (DTMs) based on airborne Light Detection and Ranging (LiDAR) that were taken shortly before and after the flood to investigate insights into geomorphic changes. ResultsBy calculating Digital Terrain Models of Difference (DoD), we are able to quantify volumetric and areal changes caused by erosional and depositional processes for different sites in the Ahr Valley. Due to the morphology of the narrow Ahr Valley, most of the erosion and deposition is located within the deeply incised canyon of the Ahr River. The comprehensive analysis reveals notable morphological modifications throughout the study area, with a calculated erosion/deposition areal ratio of 0.46 and an erosion/deposition volumetric ratio of 0.63. Our findings indicate massive deposition regarding both areal and volumetric. We selected six different locations along the Ahr Valley that showcase distinct aspects of flood-induced fluvial morpho-dynamics. Deposition occurred mainly in point bars and downstream of destroyed artificial levees, in a braided river style.ConclusionOur investigations contribute to an overview and assessment of the morphological response to the destructive flood in the Ahr Valley. The results emphasize the necessity for implementing effective flood management strategies, as most of the urban areas in the Ahr Valley were flooded. Moreover, our results provide valuable insights into the impacted areas, highlighting vulnerable locations for flood-related erosion and deposition. This information could contribute to future mitigation and protection efforts, aiding in the development of comprehensive strategies to minimize the impact of similar events in the future.

Limited Evidence of Late Quaternary Tectonic Surface Deformation in the Eastern Tennessee Seismic Zone, United States

ABSTRACT The ∼300-km-long eastern Tennessee seismic zone, United States, is the secondmost seismically active region east of the Rocky Mountains. Seismicity generally occurs below the Paleozoic fold-and-thrust belt within the Mesoproterozoic basement, at depths of 5–26 km, and earthquake magnitudes during the instrumental record have been moment magnitude (Mw) ≤4.8. Evidence of surface deformation may not exist or be difficult to detect because of the vegetated and soil-mantled landscape, landslides, locally steep topography, anthropogenic landscape modification, or long, irregular recurrence intervals between surface-rupturing earthquakes. Despite the deep seismicity, analog models indicate that accumulation of strike-slip or oblique-slip displacement at depth could be expected to propagate upward through the Paleozoic section, producing a detectable surficial signal of distributed faulting. To identify potential surface deformation, we interrogated the landscape at different spatial scales. We evaluated morphotectonic and channel metrics, such as channel sinuosity and catchment-scale hypsometry. In addition, we mapped possible fault-related topographic features on 1-m lidar data. Finally, we integrated our observations with available bedrock and Quaternary surficial mapping and subsurface geophysical data. At a regional scale, most morphotectonic and channel metrics have a strong lithologic control. Within smaller regions of similar lithology, we observe changes in landscape metrics like channel sinuosity and catchment-scale hypsometry that spatially correlate with new lineaments identified in this study and previously mapped east–west Cenozoic faults. These faults have apparent left-lateral offsets, are optimally oriented to slip in the current stress field, and match kinematics from the recent focal mechanisms, but do not clearly preserve evidence of late Pleistocene or Holocene tectonic surface deformation. Most newly mapped lineaments might be explained by either tectonic or nontectonic origins, such as fluvial or karst processes. We also reevaluated a previously described paleoseismic site and interpret that the exposure does not record evidence of late Pleistocene faulting but instead is explained by fluvial stratigraphy.

Anthropogenic nest material use in a global sample of birds.

As humans increasingly modify the natural world, many animals have responded by changing their behaviour. Understanding and predicting the extent of these responses is a key step in conserving these species. For example, the tendency for some species of birds to incorporate anthropogenic items-particularly plastic material-into their nests is of increasing concern, as in some cases, this behaviour has harmful effects on adults, young and eggs. Studies of this phenomenon, however, have to date been largely limited in geographic and taxonomic scope. To investigate the global correlates of anthropogenic (including plastic) nest material use, we used Bayesian phylogenetic mixed models and a data set of recorded nest materials in 6147 species of birds. We find that, after controlling for research effort and proximity to human landscape modifications, anthropogenic nest material use is correlated with synanthropic (artificial) nesting locations, breeding environment and the number of different nest materials the species has been recorded to use. We also demonstrate that body mass, range size, conservation status and brain size do not explain variation in the recorded use of anthropogenic nest materials. These results indicate that anthropogenic materials are more likely to be included in nests when they are more readily available, as well as potentially by species that are more flexible in their nest material choice.

Abstract 4049: The beta-carboline Harmine sensitives microsatellite stable colorectal cancer mouse model to anti-PD-1 through upregulating MHC-I dependent antigen presentation machinery and improving the infiltration of CD8 T cells into the tumor microenvironment

Abstract Colorectal cancer (CRC) is the most common gastrointestinal cancer and the third leading cause of cancer-related death in both sexes. Mismatch repair deficient/microsatellite instability-high (MSI) colorectal cancer patients benefit from immune checkpoint blockades (ICBs)-based immunotherapy; however, this benefit has not been translated into microsatellite stable (MSS) colorectal cancer. It has been proposed that the loss of major histocompatibility complex class I (MHC-I) in tumor cells limits the use of ICB in colorectal cancer. Harmine displays a number of biological and pharmacological properties affecting the immune response and tumor phenotype. Here, we assessed the impact of combining harmine, also referred to as ACB1801 molecule, on improving the responsiveness of ICB in the mouse MSS CRC model. Our results showed that combining ACB1801 significantly improves the therapeutic benefit of anti-PD-1 in the CT26 CRC mouse model by decreasing tumor growth and improving the survival of tumor-bearing mice. The improvement of anti-PD-1-based therapy by combining ACB-1801 was associated with a modification of the immune landscape of tumors as evidenced by an increase in the CD8+ T cells and a decrease in the Treg infiltrations into the tumor microenvironment. By profiling the cytokine/chemokine network, we showed that ACB1801 induced the expression and the release of the proinflammatory chemokine CXCL10 by CRC tumor cells in vitro, which could be responsible for driving CD8+ T cells to the tumor microenvironment. Mechanistically, we showed that ACB1801 increased the expression of MHC-I genes, including TAP1 and TAPASIN, and improved the antigen loading on MHC-I in CRC cells in vitro. Overall, our study highlights the value of combining ACB1801 and anti-PD-1 as a therapeutic opportunity to convert MSS colorectal cancer into an “immune hot” cancer, which may define the future treatment paradigm of colorectal cancer for which there is a great unmet need. Citation Format: Ruize Gao, Kris Van Moer, Coralie Pulido, Anaïs Oudin, Diane Murera, Teresa L. Ramos, Margaux Poussard, Andreas Schläpfer, Annette Ives, Christian Auclair, Bassam Janji. The beta-carboline Harmine sensitives microsatellite stable colorectal cancer mouse model to anti-PD-1 through upregulating MHC-I dependent antigen presentation machinery and improving the infiltration of CD8 T cells into the tumor microenvironment [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 4049.

Anthropogenic activities facilitate temporal overlaps and spatial partitions among sympatric canids in a human-modified landscape of Bulgaria

Mammalian carnivores (order Carnivora) occupy higher trophic levels in many terrestrial ecosystems, shaping community structures via direct predation and trophic cascades. The interference competition among sympatric carnivores (i.e., aggression and killing) has a major role in the intraguild interactions in which the large-dominant predators typically constrain behaviors and resource use patterns by exploitative (i.e., resource) competitions as well as the survival of smaller subordinate species by interference (i.e., direct) competitions. The free-roaming dog, Canis lupus familiaris, has been introduced worldwide and the species affects regional biodiversity, including native carnivore species. Using camera trapping, we investigated the temporal and spatial overlaps between the free-roaming dog and two wild canids, the golden jackal (Canis aureus) and the red fox (Vulpes vulpes), in different landscapes of central Bulgaria. We predict their interference interactions, namely that the dog, as the largest competitor, suppresses the golden jackal with intermediate body size, and, in turn, the golden jackal suppresses the smaller red fox. In mountainous forest landscapes where the free-roaming dog was absent or scarce, the golden jackal and red fox partitioned their diel activities, while there were moderate levels of spatial overlap. In agricultural lowlands where dogs were abundant and anthropogenic disturbances were relatively high, all three canids were primarily nocturnal with high temporal overlap, probably owing to human activities (e.g., hunting) in the daytime. The golden jackal was spatially separated from the dog, whereas the red fox spatially overlapped with large dogs. Our results indicated that, in human-modified landscapes with scattered forests and shrubs, spatial partitioning between the free-roaming dog and the golden jackal at fine spatial scales facilitates their sympatry by decreasing the probability of direct encounters. Furthermore, our findings also indicate that spatiotemporal interactions between the golden jackal and the red fox can change in association with various human disturbances, e.g., landscape modifications, human activities, and the introduction of dogs.

Drivers for soil organic carbon stabilization in Elbe River floodplains

AbstractBackgroundFloodplains play an important role in the global carbon (C) cycle, particularly due to their large soil organic carbon (SOC) storage potential. However, a heterogeneous microrelief and anthropogenic landscape modifications complicate the understanding of stabilization processes of SOC in floodplains.AimDetermining the predominant drivers for SOC stabilization in Elbe River floodplain soils.MethodsWe measured SOC density fractions, microbial biomass, and mineralization characteristics in top‐ and subsoils of eight floodplain sites of the lower middle Elbe River.ResultsThe heavy fraction (HF) was the most important SOC pool, with a contribution of >64% at both depth intervals. With soil depth, HF pool size increased and the occluded light fraction (oLF) decreased, whereas the free LF (fLF) stayed the same. The contribution of the HF to SOC was positively related to fine texture (R2 = 0.64). Mineralizable C was negatively related to fine texture at both depth intervals. Both results suggest a positive effect of fine texture on SOC stabilization. The metabolic quotient was related to the amount of available SOC in the topsoil, but no relation was found in the subsoil. However, in top‐ and subsoil, the mineralization rate constant was positively related to the C/N ratios of the fLF and the oLF, indicating that the quality of fresh plant litter is an important energy source for microbial mineralization.ConclusionSedimentation of fine‐textured material is the most important driver for SOC stabilization rather than fresh plant litter input. Thus, SOC stabilization strongly depends on relief, flooding, and sedimentation.

A pan-cancer analysis of the oncogenic and immunological roles of transglutaminase 1 (TGM1) in human cancer

BackgroundThere is currently a limited number of studies on transglutaminase type 1 (TGM1) in tumors. The objective of this study is to perform a comprehensive analysis across various types of cancer to determine the prognostic significance of TGM1 in tumors and investigate its role in the immune environment.MethodPan-cancer and mutational data were retrieved from the TCGA database and analyzed using R (version 3.6.4) and its associated software package. The expression difference and prognosis of TGM1 were examined, along with its correlation with tumor heterogeneity, stemness, mutation landscape, and RNA modification. Additionally, the relationship between TGM1 expression and tumor immunity was investigated using the TIMER method.ResultsTGM1 is expressed differently in various tumors and normal samples and is associated with the overall survival and progression-free time of KIRC, ACC, SKCM, LIHC, and STES. In LICH, we found a negative correlation between TGM1 expression and 6 indicators of tumor stemness. The mutation frequencies of BLCA, LIHC, and KIRC were 1.7%, 0.3%, and 0.3% respectively. In BLCA and BRCA, there was a significant correlation between TGM1 expression and the infiltration of CD4 + T cells, CD8 + T cells, neutrophils, and dendritic cells.ConclusionTGM1 has the potential to serve as both a prognostic marker and a drug target.

Drought influences habitat associations and abundances of birds in California's Central Valley

AbstractAimAs climate change increases the frequency and severity of droughts in many regions, conservation during drought is becoming a major challenge for ecologists. Droughts are multidimensional climate events whose impacts may be moderated by changes in temperature, water availability or food availability, or some combination of these. Simultaneously, other stressors such as extensive anthropogenic landscape modification may synergize with drought. Useful observational models for guiding conservation decision‐making during drought require multidimensional, dynamic representations to disentangle possible drought impacts, and consequently, they will require large, highly resolved data sets. In this paper, we develop a two‐stage predictive framework for assessing how drought impacts vary with species, habitats and climate pathways.LocationCentral Valley, California, USA.MethodsWe used a two‐stage counterfactual analysis combining predictive linear mixed models and N‐mixture models to characterize the multidimensional impacts of drought on 66 bird species. We analysed counts from the eBird participatory science data set between 2010 and 2019 and produced species‐ and habitat‐specific estimates of the impact of drought on relative abundance.ResultsWe found that while fewer than a quarter (16/66) of species experienced abundance declines during drought, nearly half of all species (27/66) changed their habitat associations during drought. Among species that shifted their habitat associations, the use of natural habitats declined during drought while use of developed habitat and perennial agricultural habitat increased.Main ConclusionsOur findings suggest that birds take advantage of agricultural and developed land with artificial irrigation and heat‐buffering microhabitat structure, such as in orchards or parks, to buffer drought impacts. A working lands approach that promotes biodiversity and mitigates stressors across a human‐induced water gradient will be critical for conserving birds during drought.

Diversity of disease-carrying mosquito vectors in potential areas of dengue fever in Palembang City, South Sumatra, Indonesia

Abstract. Listiono H, Damiri N, Kamaluddin T, Irsan C. 2024. Diversity of disease-carrying mosquito vectors in potential areas of dengue fever in Palembang City, South Sumatra, Indonesia. Biodiversitas 25: 474-481. Mosquitoes (Diptera: Culicidae) are biting insects commonly found in many areas on earth in subtropics and tropics, especially around residential areas. So far, more than 2500 species have been recorded from various parts of the world. They are the main vectors of many diseases in humans and livestock caused by viruses and parasites. Mosquito diversity and disease transmission are influenced by landscape modifications, namely vectors and pathogens. The study aimed to evaluate the distribution, prevalence and abundance of mosquito species in potential dengue fever districts in Palembang city. The study was conducted in 2 two sub-districts in Palembang City, namely Gandus and Seberang Ulu I that represent housing environment, schools and gardens. Mosquito capture was done during three periods 06.00-10.00, 15.00-18.00, and 19.00-21.00 to align with their active hours. This involved active human landing collection method. 200 ovitraps were set up in both regions to assess the density of mosquitoes. The results showed that the diversity index values in Gandus and the Seberang Ulu I sub-districts were 0.781 and 0.756, respectively. Both values were categorized as low diversity index. Furthermore, the dominance index in Gandus and Seberang Ulu 1 sub-district were 0.105 and 0.031, respectively. It meant that there were no dominant mosquito species in both sub-districts. Based on the results of Independent t-test analysis, it was found that the average number of mosquito populations in Gandus sub-district was 25.07 and Seberang Ulu I sub-district was 13.29. From the results of the analysis it was found that there was no difference in average number of mosquito populations between Gandus and Seberang Ulu I sub-district, with a p-value of 0.367. Further research needs to be directed at uncovering mosquito distribution patterns and their potential as disease vectors, the risk of infectious diseases, and the need for further prevention to reduce the number of disease incidences.