Understanding the spatial distribution of evapotranspiration (ET) and other surface energy fluxes in tree crops is vital for optimizing precision irrigation management practices. The land surface temperature (LST) is the primary input to estimate ET at field scale, but currently, available satellite data have a coarse resolution, which is not suitable for estimating ET at the required spatiotemporal scale at the field level. The development of unmanned aerial vehicles (UAVs) or airborne platforms during the last two decades has enabled the acquisition of LST and subsequent derivation of ET at the finer spatial resolution of up to a few centimeters due to the advances in low-weight thermal cameras onboard these platforms. In this study, the disaggregation methodology was applied to Landsat imagery based on aerial imagery collected at 0.5 m resolution over almond orchards in the Sacramento Valley of California, USA, to estimate ET at the orchard scale. The TsHARP algorithm was applied to sharpen the Landsat LST images (30 m spatial resolution) to target resolutions of 0.5, 10, 20 m. Also, the simple average aggregation technique was used to aggregate the aerial imagery (0.5 resolution) to 10, 20, and 30 m scales. Two Source Energy Balance (TSEB) model was used to estimate spatially distributed ET fluxes by combining the ground-based meteorological data for aggregated and disaggregated LST images. The estimated ET fluxes were compared to the measured eddy covariance fluxes, and then the aggregation and disaggregation processes were evaluated at 0.5, 10, 20, and 30 m spatial scales. At four spatial scales (0.5, 10, 20, and 30 m) aggregation and disaggregation derived ET values were highly correlated with the measured eddy covariance fluxes within the source footprint area. Furthermore, both aggregation and disaggregation approaches demonstrated similar ET spatial patterns within the study area, with slight variations observed under the Landsat 30 m resolution. The results indicated that TsHARP downscaled high-resolution imagery provides optimal spatial resolution for mapping and analyzing ET at the plant to orchard scale, with a 95% confidence interval and an RMSE of 0.43 mm/day. Overall, the results suggest that high spatial resolution imagery disaggregated from Landsat was the best alternative for estimating ET fluxes from the plant to the orchard scale, which is beneficial for precision irrigation management in orchards, but the framework can also be applied to other precision agriculture applications.