Crop Landraces Research Articles

Cultivated plants and their wild relatives of Central Russia and the North Caucasus (results of a collecting mission of 2023)

In 2023, field explorations of cultivated plants and their wild relatives were carried out in the regions of the Volga Upland, the western part of the Caspian Lowland and the Stavropol Upland with the aim of further replenishing seed and herbarium collections of VIR.A total of 525 samples of CWR and cultivated plants were collected, including 200 are herbarium sheets, 323 seed samples of cultivated plants and CWR, and a couple of cuttings. The collected material encompasses 145 species, including 91 cultivars. Seeds of the cultivars were purchased at markets, in supermarket chains and from local residents. The following areas were examined in detail: the left bank of the Lower Volga (Peschany anabranch, Kigach anabranch, as well as the “Novaya Roshcha” protected area). In the Republic of Ingushetia the team collected samples of cherry plum, Caucasian pear, barberry, viburnum, Lonicera orientalis, sea buckthorn, raspberry, and Malus orientalis, which local residents use in their diet. Also, samples of spicy herbs such as savory, catnip, oregano, thyme, etc. were collected. Hops were sampled in the Tambov and Volgograd provinces, the Republics of Ingushetia, North Ossetia and Adygea, from abandoned household plots in Botanika village in Gulkevichi District (Krasnodar Territory). The exploration in vicinities of Derbent and of the wall of Naryn-Kala fortress in Derbent resulted in collecting a rare specimen of Secale sylvestre Host., which previously used to be a fairly common plant in the North Caucasus, but is very rare now. Vegetable crops were collected along the entire route of the collecting mission. It was not always possible to collect seeds, so a part of the material enriched the herbarium collection. The collected material reflects the use of a vast range of vegetable and spicy plant species by the local population.Collecting missions help to understand which species are of primary importance for the conservation of natural processes, biodiversity and the maintenance of local ecosystems. The genetic diversity of a region is a key object of research for geneticists and plant breeders. Landraces of vegetable, cereal and other crops may have unique properties, such as adaptation to local climate conditions or disease resistance. Work on the mobilization and conservation of the plant genetic diversity is the basis for modern breeding and genetic research.

The Dual Role of Culture in Evolutionary Play: Anthropogenic Expansion Vs Destruction of Biodiversity

A large number of Green discourse blames the human species for the current ecological crisis. However, this description of humanity as the ecocidal culprit serves to conceal the role of humans, in both past and contemporary pre-industrial societies, as custodians of biodiversity. Indigenous societies are known to have conserved their natural resource base for posterity, by instituting cultural norms and institutions against exhaustive resource use. In addition, pre-industrial societies also increased biodiversity on taxic and genetic levels, by domestication of many wild biota, and on the ecosystem level with agroforestry. While Darwin gave much importance to the process of domestication of plants and animals by means of artificial selection, modern science and agriculture curricula tend to neglect this aspect of the history of human civilization. The novel taxa, created in the process of domestication, are characterised by many morphological and behavioural traits never found in the wild progenitor species. Further selection of favourable traits of the new species created an abundance of distinctive crop landraces and animal breeds. The increment in diversity at the ecosystem, taxic and genetic levels, by the process of domestication and in ancient agroforestry systems, began to reverse with industrial development over the past two centuries. Indeed, industrial development has been the chief driver of the continuing process of biodiversity erosion and habitat loss worldwide. Industrial agricultural systems, signposted by the Green Revolution (GR), has severely truncated the on-farm crop species and genetic diversity, which characterised traditional multi-crop farming and agroforestry systems in native agrarian cultures. Over the past six decades, the continual replacement of hundreds of landraces with a handful of GR cultivars, combined with the institutional apathy toward in situ landrace conservation efforts, has led to the disappearance of the importance of genetic purity of landraces from breeding programs and heirloom crop conservation discourse. Most of the modern farmers, predominantly dependent on the industrial supply of crop seeds, have forgotten the methods of genetic purity maintenance, resulting in the rapid loss of the hundreds of crop landraces with distinctive properties, which were selected centuries ago for diverse agronomic, gustatory, and aesthetic qualities. A recognition of the value of the custodian role of ecosystem people in creating and conserving biodiversity, vis-a-vis the current industrial decimation of biodiversity on all levels, will likely promote biodiversity conservation ethos in modern societies, and the value of genetic purity of the extant crop landraces.

Crop Landraces and Indigenous Varieties: A Valuable Source of Genes for Plant Breeding.

Landraces and indigenous varieties comprise valuable sources of crop species diversity. Their utilization in plant breeding may lead to increased yield and enhanced quality traits, as well as resilience to various abiotic and biotic stresses. Recently, new approaches based on the rapid advancement of genomic technologies such as deciphering of pangenomes, multi-omics tools, marker-assisted selection (MAS), genome-wide association studies (GWAS), and CRISPR/Cas9 gene editing greatly facilitated the exploitation of landraces in modern plant breeding. In this paper, we present a comprehensive overview of the implementation of new genomic technologies and highlight their importance in pinpointing the genetic basis of desirable traits in landraces and indigenous varieties of annual, perennial herbaceous, and woody crop species cultivated in the Mediterranean region. The need for further employment of advanced -omic technologies to unravel the full potential of landraces and indigenous varieties underutilized genetic diversity is also indicated. Ultimately, the large amount of genomic data emerging from the investigation of landraces and indigenous varieties reveals their potential as a source of valuable genes and traits for breeding. The role of landraces and indigenous varieties in mitigating the ongoing risks posed by climate change in agriculture and food security is also highlighted.

Towards a practical threat assessment methodology for crop landraces.

Crop landraces (LR), the traditional varieties of crops that have been maintained for millennia by repeated cycles of planting, harvesting, and selection, are genetically diverse compared to more modern varieties and provide one of the key components for crop improvement due to the ease of trait transfer within the crop species. However, LR diversity is increasingly threatened with genetic erosion and extinction by replacement with improved cultivars, lack of incentives for farmers to maintain traditional agricultural systems, and rising threats from climate change. Their active conservation is necessary to maintain this critical resource. However, as there are hundreds of thousands of LR and millions of LR populations for crops globally, active conservation is complex and resource-intensive. To assist in implementation, it is useful to be able to prioritise LR for conservation action and an obvious means of prioritisation is based on relative threat assessment. There have been several attempts to propose LR threat assessment methods, but none thus far has been widely accepted or applied. The aim of this paper is to present a novel, practical, standardised, and objective methodology for LR threat assessment derived from the widely applied IUCN Red Listing for wild species, involving the collation of time series information for LR population range, LR population trend, market, and farmer characteristics and LR context information. The collated information is compared to a set of threat criteria and an appropriate threat category is assigned to the LR when a threshold level is reached. The proposed methodology can be applied at national, regional, or global levels and any crop group.

Physiological traits contribute to growth and adaptation of Mexican maize landraces.

Local adaptation of populations results from an interplay between their environment and genetics. If functional trait variation influences plant performance, populations can adapt to their local environment. However, populations may also respond plastically to environmental challenges, altering phenotype without shifting allele frequencies. The level of local adaptation in crop landraces and their capacity for plasticity in response to environmental change may predict their continued utility to farmers facing climate change. Yet we understand little about how physiological traits potentially underlying local adaptation of cultivars influence fitness. Farmers in Mexico-the crop center of origin for maize-manage and rely upon a high diversity of landraces. We studied maize grown in Chiapas, Mexico, where strong elevational gradients cover a relatively small geographic area. We reciprocally transplanted 12 populations sourced from three elevational zones (600, 1550 and 2150 m) back into those elevations for two years using a modified split-split plot design to model effects of environment, genetics, and their interaction. We studied physiological and growth traits, including photosynthetic rate, stomatal conductance, stomatal density, relative growth rate (RGR), and seed production. Maize fitness showed indications of local adaptation with highland and midland types performing poorly at warmer lowland locations, though patterns depended on the year. Several physiological traits, including stomatal conductance, were affected by G x E interactions, some of which indicated non-adaptive plastic responses with potential fitness implications. We discerned a significant positive relationship between fitness and relative growth rate. Growth rates in highland landraces were outperformed by midland and lowland landraces grown in high temperature, lowland garden. Lowland landrace stomatal conductance was diminished compared to that of highland landraces in the cooler highland garden. Thus, both adaptive and non-adaptive physiological responses of maize landraces in southern Mexico may have implications for fitness, as well as responses to climate change.

Ethnolinguistic associations and genetic diversity of rice landraces in Nagaland, India

Societal Impact StatementPreserving and conserving crop landraces, tended by indigenous farming communities, is crucial for future food security. This research focused on rice landrace diversity in the north‐eastern Himalayan region of Nagaland, India, where Naga communities cultivate rice according to their dietary and cultural preferences. Rice diversity is closely linked to the region's ethnolinguistic and ecological variety. On‐farm conservation, in collaboration with indigenous communities, is imperative to protect these germplasm resources. Involving these communities actively in conservation efforts will safeguard their traditional knowledge, endorse sustainable farming practices, and enhance the resilience of local agricultural systems.Summary Understanding the genetic diversity and cultural significance of crop landraces is crucial for their conservation and sustainable utilization. This study focused on rice landraces from Nagaland in north‐eastern India to assess their genetic diversity and explore their associations with ethnolinguistic groups. We collected 78 rice landraces from Nagaland and a small part of Manipur and conducted microsatellite genotyping for genetic analysis. We integrated social anthropology and population genetics analyses of rice landraces to glean insights into the genetic diversity, population structure, and ethnolinguistic history of rice cultivation in Nagaland. The study revealed the rich cultural significance of rice landraces among the Nagas. Farmers practiced small‐scale subsistence farming, maintaining diverse rice landraces. Naming conventions were based on factors such as seed source, color, grain type, and ecological suitability. Rice landraces played important roles in ethnic cultures, festivals, and religious ceremonies. Genetic analysis identified significant diversity, with 277 alleles across 69 loci and a moderate gene diversity of 0.57. Two distinct sub‐populations were identified, with one dominated by Chakhesang and Angami Nagas and the other by Sümi and Lotha accessions. Differentiation was observed between lowland and upland cultivars, with one sub‐population comprising exclusively lowland varieties. Cultural factors and cultivation practices influenced population differentiation, with ethnicity and ecotype having a significant impact. The study also highlighted the correlation between ethnolinguistic differentiation and the indica–japonica structuring of rice landraces. Different ethnic groups in Nagaland had distinct cultivation practices, contributing to genetic differentiation. Overall, this research emphasizes the need to preserve rice landraces and associated traditional knowledge for future improvements and cultural heritage conservation. It provides insights into genetic diversity, cultural significance, and the relationship between genetic diversity, cultural practices, and agricultural traditions.

Differences in mucilage properties and stomatal sensitivity of locally adapted Zea mays in relation with precipitation seasonality and vapour pressure deficit regime of their native environment.

With ongoing climate change and the increase in extreme weather events, especially droughts, the challenge of maintaining food security is becoming ever greater. Locally adapted landraces of crops represent a valuable source of adaptation to stressful environments. In the light of future droughts-both by altered soil water supply and increasing atmospheric water demand (vapor pressure deficit [VPD])-plants need to improve their water efficiency. To do so, plants can enhance their access to soil water by improving rhizosphere hydraulic conductivity via the exudation of mucilage. Furthermore, plants can reduce transpirational water loss via stomatal regulation. Although the role of mucilage and stomata regulation on plant water management have been extensively studied, little is known about a possible coordination between root mucilage properties and stomatal sensitivity as well as abiotic drivers shaping the development of drought resistant trait suits within landraces. Mucilage properties and stomatal sensitivity of eight Mexican landraces of Zea mays in contrast with one inbred line were first quantified under controlled conditions and second related to water demand and supply at their respective site of origin. Mucilage physical properties-namely, viscosity, contact angle, and surface tension-differed between the investigated maize varieties. We found strong influences of precipitation seasonality, thus plant water availability, on mucilage production (R 2= .88, p< .01) and mucilage viscosity (R 2= .93, p< .01). Further, stomatal sensitivity to increased atmospheric water demand was related to mucilage viscosity and contact angle, both of which are crucial in determining mucilage's water repellent, thus maladaptive, behavior upon soil drying. The identification of landraces with pre-adapted suitable trait sets with regard to drought resistance is of utmost importance, for example, trait combinations such as exhibited in one of the here investigated landraces. Our results suggest a strong environmental selective force of seasonality in plant water availability on mucilage properties as well as regulatory stomatal effects to avoid mucilage's maladaptive potential upon drying and likely delay critical levels of hydraulic dysfunction. By this, landraces from highly seasonal climates may exhibit beneficial mucilage and stomatal traits to prolong plant functioning under edaphic drought. These findings may help breeders to efficiently screen for local landraces with pre-adaptations to drought to ultimately increase crop yield resistance under future climatic variability.

Evaluation of Cowpea Landraces under a Mediterranean Climate.

Cowpea (Vigna unguiculata (L.) Walp.) yield is strongly influenced by environmental conditions. Average seed yield can decrease to a great extent when drought conditions occur, especially when they prevail during flowering and seed filling periods. Identifying genotypes presenting yield stability is one of the most important breeding goals. Local varieties or crop landraces are genetic resources that, despite exhibiting intermediate yield production capacity, present high yield stability in low-input cropping systems. The objective of this study was therefore to evaluate five selected cowpea landraces originated from different Greek islands under Mediterranean climatic conditions. A complete randomized block design with four replications was used during three consecutive cropping seasons. Many phenological and agronomic traits studied showed statistically significant genotype × experimental year interaction, while there was a strong experimental year effect. Among the landraces studied, local population VG23 from Kythira Island was the most productive under the experimental climatic and soil conditions, while local population VG2 from Lemnos Island was characterized by low seed productivity. Conclusively, our study showed that VG23 landrace is a promising genetic material to be used for seed yield improvement.

Trends, challenges and opportunities in the in situ conservation of cereal landraces in Scottish islands

Landraces are traditional crop varieties that often have special adaptations to the farming environment in which they have evolved and are therefore a valuable source of useful traits for plant breeders. In most agriculturally advanced countries, landraces of the main crops have generally been superseded by modern varieties. An exception to this in the United Kingdom is the cultivation on the Scottish archipelagos of Orkney, Shetland and the Outer Hebrides of three cereal landraces: bere, a 6-row barley (Hordeum vulgare), small oat (Avena strigosa) and Hebridean rye (Secale cereale). Our study focused on trends in their cultivation and use over the past 20 years. In the Outer Hebrides, a mixture of all three has continued to be grown on more than 200ha for feed because of its tolerance of nutrient-deficient sandy soils. Future cultivation is threatened, however, by damage from geese and deer, especially to fields used for seed production. In Orkney and Shetland, only bere and small oat are grown, and always as sole crops. The area of bere has increased in Orkney, from about 10ha in 2004 to almost 75ha in 2020 and has been driven by two supply chains producing bere for milling and malting. However, small oat in Orkney, and both small oat and, especially bere, in Shetland have been grown by very few farmers since 2018 and are at serious risk of being lost from cultivation. We discuss these results in the context of measures to support greater on-farm cultivation of these landraces.

Traditional agroforestry of Angami Nagas of Nagaland state, India- a quantitative assessment of socio-cultural values and agrobiodiversity

Nhalie (shifting cultivation) and teizie (home garden) are the major forms of traditional agroforestry practices of Angami Nagas in Kohima district of Nagaland. They are characterized by high socio-cultural significance and prevalence of high degree of agrobiodiversity. The present study aims to assess agrobiodiversity and socio-cultural values of nhalie agroforestry of Angami Nagas in Kohima district of Nagaland, India. This study collected primary data from 60 households across five Angami villages by using methods of group discussion and participatory interview. Teizie and nhalie had higher average cultural ecosystem service values of 7.5 and 7.1, respectively. In term of economic ecosystem services, nhalie cultivation was perceived to be an important agroforestry practice with social use value (SUV) of 52.21. A total of 136 landraces of traditional crops belonging to 36 species were recorded. Two scented rice landraces (kethselha and rüluoo) and one sticky landrace (nhalenya) were reported. High overall Simpson’s Diversity Index value of 0.72 revealed high level of crop species diversity in nhalie agroforestry of Angami Nagas. Rice was the most common crop with the highest genetic diversity (22 landraces) followed by pumpkin, squash, taro, cucumber and maize. Nhalie agroforestry could serve as the major reservoir of traditional crop species and contribute to the conservation of these valuable traditional crop species on-farm. Further, the existing agrobiodiversity managed with the traditional wisdom of Angami Nagas in nhalie agroforestry systems is required to be preserved and disseminated for sustainable use of bioresources.

Adapting to Social–Ecological Risks to the Conservation of a Muskmelon Landrace in India

Crop landraces are vanishing alarmingly worldwide, posing serious risks to the livelihoods of the resource-poor farmers; this study, conducted using ‘vulnerability’ and ‘resilience theory’ frameworks, sought to delineate social–ecological, climatic and policy hindrances to the conservation of a muskmelon landrace ‘Jaunpuri Netted’ traditionally grown in eastern Uttar Pradesh, India. Our results showed that the blue bull menace, market constraints and erratic rainfall have gradually emerged as severe stresses to the conservation of this muskmelon landrace. Yet, a set of enablers including relative ease in crop management, pleasant fruit taste, perceived livelihood opportunities and the cultural legacy seem to offset these stresses, at least partly, keeping the farmers engaged in muskmelon cultivation. The Tobid regression analysis revealed that educated farmers with large landholdings were likely to grow muskmelon on relatively small acreages, and that market constraints, blue bull menace and erratic rainfall are the major future risks to the muskmelon-based livelihoods. A growing obsession with higher fruit yields has led to the virtual eclipse of traditional crop management practices, further enhancing the vulnerability of muskmelon growers. Addressing these challenges requires some major changes to the ways in which the muskmelon crop is managed and traded. While muskmelon growers need to revisit the present chemical-intensive practices, adequate research and policy support remain requisite to unveiling the unique nutraceutical properties of this muskmelon landrace, promoting organic farming, reviving seed-based business opportunities, and creating strong market linkages to enhance the livelihood resilience of the muskmelon growers.

Agro-Morphological Characterization and Nutritional Profiling of Traditional Himalayan Crop Landraces for Their Promotion Toward Mainstream Agriculture.

The northwest Indian Himalayas are often regarded as a biological hotspot for the presence of rich agro-biodiversity harboring locally adapted traditional crop landraces facing utter neglect owing to modern agricultural systems promoting high-yielding varieties. Addressing this challenge requires extricating the potential of such cultivars in terms of agro-morphological and nutritional attributes. In this study, 29 traditional crop landraces of maize (11), paddy (07), finger millet (03), buckwheat (05), and naked barley (03) were characterized and evaluated for target traits of interest. In maize, Chitkanu emerged as an early maturing landrace (107 days) with high concentrations of zinc (Zn), iron (Fe), and potassium (K), and Safed makki showed the highest 100-seed weight (28.20 g). Similarly, Bamkua dhan exhibited high concentrations of K and phosphorus (P), and Lamgudi dhan showed a high protein content (14.86 g/100 g) among paddy landraces. Ogla-I and Phapra-I showed high contents of protein (14.80 g/100 g) and flavonoids (20.50 mg/g) among buckwheat landraces, respectively, followed by Nei-I, which exhibited the highest protein content (15.66 g/100 g) among naked barley landraces. Most of the target traits varied significantly (p < 0.05) among evaluated samples, except those associated with finger millet landraces. The grouping pattern obtained by principal component analysis (PCA) and multidimensional scaling (MDS) was congruent with the geographical relationship among the crop landraces. This study led to the identification of elite crop landraces having useful variations that could be exploited in plant breeding programs and biofortification strategies for future crop improvement. Our endeavor would aid in conserving the depleting Himalayan agro-biodiversity and promoting versatile traditional crops toward mainstream agriculture vis-à-vis future nutritional security.

Survey and conservation of crop landraces in northwest Syria

Syria lies at the heart of the Fertile Crescent – one of the centres of diversity of staple crops such as wheat, barley, chickpea and lentil. The country has historically been rich in agrobiodiversity, including crop landraces valued for their nutritional and culinary qualities, as well as for their resilience. With their cultivation already in decline before the start of the Syrian crisis in 2011, this study was undertaken to assess the current status of crop landraces in northwest Syria, and to initiate an ex situ conservation programme. We found a significant decline in the number of landraces in cultivation, indicating a loss of locally adapted genetic diversity. Influencing factors include insufficient seed supply, competition with commercial hybrids, falling market demand and neglect by relevant government authorities. Despite not seeing conservation as their responsibility, the participating farmers were supportive of landrace conservation and willingly contributed seeds for ex situ conservation.

State of ex situ conservation of landrace groups of 25 major crops

Crop landraces have unique local agroecological and societal functions and offer important genetic resources for plant breeding. Recognition of the value of landrace diversity and concern about its erosion on farms have led to sustained efforts to establish ex situ collections worldwide. The degree to which these efforts have succeeded in conserving landraces has not been comprehensively assessed. Here we modelled the potential distributions of eco-geographically distinguishable groups of landraces of 25 cereal, pulse and starchy root/tuber/fruit crops within their geographic regions of diversity. We then analysed the extent to which these landrace groups are represented in genebank collections, using geographic and ecological coverage metrics as a proxy for genetic diversity. We find that ex situ conservation of landrace groups is currently moderately comprehensive on average, with substantial variation among crops; a mean of 63% ± 12.6% of distributions is currently represented in genebanks. Breadfruit, bananas and plantains, lentils, common beans, chickpeas, barley and bread wheat landrace groups are among the most fully represented, whereas the largest conservation gaps persist for pearl millet, yams, finger millet, groundnut, potatoes and peas. Geographic regions prioritized for further collection of landrace groups for ex situ conservation include South Asia, the Mediterranean and West Asia, Mesoamerica, sub-Saharan Africa, the Andean mountains of South America and Central to East Asia. With further progress to fill these gaps, a high degree of representation of landrace group diversity in genebanks is feasible globally, thus fulfilling international targets for their ex situ conservation.

The Neglected Traditional Enset (Ensete ventricosum) Crop Landraces for the Sustainable Livelihood of the Local People in Southern Ethiopia.

Enset (Ensete ventricosum, Musaceae) is a neglected traditional multipurpose crop plant critical for Ethiopian food security. It has drawn a lot of attention in the last few years. This study was undertaken on the morphological diversity among the enset landraces and their cultural use for the livelihood of the people in Southern Ethiopia. The study was administered in four purposively selected kebeles of the Mareka District. A total of 145 individuals were interviewed using semistructured interviews, and field observation has also occurred. The descriptors for enset developed by the International Board for Plant Genetic Resources were used to measure the morphological features. This study found twenty-two enset landraces. Landraces were categorized into five groups based on their morphological trait variability. The highest mean was in cluster five, while the lowest was in cluster three. The highest landrace diversity was found in Ocha (n = 2.28) and the lowest in Guta (n = 2.17). This study confirmed that the study area has a diverse range of ecosystems. However, a reduction in production and the loss of some landraces were observed. As a result, the protection and preservation of enset landraces must be a priority for all responsible entities.

Theoretical and experimental assessment of genome-based prediction in landraces of allogamous crops

Discovery and enrichment of favorable alleles in landraces are key to making them accessible for crop improvement. Here, we present two fundamentally different concepts for genome-based selection in landrace-derived maize populations, one based on doubled-haploid (DH) lines derived directly from individual landrace plants and the other based on crossing landrace plants to a capture line. For both types of populations, we show theoretically how allele frequencies of the ancestral landrace and the capture line translate into expectations for molecular and genetic variances. We show that the DH approach has clear advantages over gamete capture with generally higher prediction accuracies and no risk of masking valuable variation of the landrace. Prediction accuracies as high as 0.58 for dry matter yield in the DH population indicate high potential of genome-based selection. Based on a comparison among traits, we show that the genetic makeup of the capture line has great influence on the success of genome-based selection and that confounding effects between the alleles of the landrace and the capture line are best controlled for traits for which the capture line does not outperform the ancestral population per se or in testcrosses. Our results will guide the optimization of genome-enabled prebreeding schemes.

An updated checklist of plant agrobiodiversity of northern Italy

Northern Italy is an area particularly suitable for the cultivation of different crops. It used to be characterized by a high agrobiodiversity. However, it is estimated that over the last decades, this area experienced a genetic erosion of more than 90%, resulting in the loss of many traditional plant genetic resources (PGR). Nevertheless, recent surveys found an unexpectedly high agrobiodiversity which remains. The presented study aims to provide a complete overview of plant agrobiodiversity (in terms of crop landraces, old cultivars and neglected crops) in northern Italy using a mixed approach of literature research and ethnobotanical field surveys. In addition, this paper aims to investigate on farm and ex situ conservation activities performed in the study area. 810 entities were found, of which 26 were identified as neglected crops, 377 as landraces (plus 124 doubtful landraces) and 99 as old cultivars (plus 184 doubtful old cultivars). 248 entities were here recorded for the first time through field surveys. It clearly emerges that by using an ethnobotanical approach, several PGR can still be found in the study area especially in small farms and home gardens. We describe hotspots of crop diversity and areas in which there is still a lack of knowledge of local agrobiodiversity. Only 43% of the PGR recorded are conserved long-term in seed banks, underlying the fact that great efforts are needed to ensure the ex situ conservation of PGR in northern Italy. Careful interventions are needed to enhance the on-farm conservation status of the crop diversity reported here by enhancing its demand and its accessibility.

Traditional uses of multi-cob bearing Murlimakai primitive maize landrace from Sikkim state, India

Sikkim is one of the most beautiful tourist hill states and land of traditional landraces of many crops and with incredible agro-biodiversity treasure. Multi-cob baring murlimakai is used for various purposes such as mouthwatering delicious foods preparation such as popcorn, chapati, roti, chyakdung. Makkaiko chamal, makkaiko pitho, makkaiko laddu and makkaiko dalla. Attractive artifacts mats, baskets, fruits and vegetable container baskets, bags and hats are prepared from ears and local beverage jhand and rakshi is prepared from the grans. Immediate conservation of this unique multi-cob bearing landrace either ex situ or in situ is necessary for future needs.

The Erosion of Biodiversity and Culture

The decimation of biodiversity at the species, genetic, and ecosystem levels as a direct consequence of the industrial resource use mode is well documented in human ecology and conservation literature. Not only wild biota but also domesticated crop landraces have been pushed to extinction by industrial land-use systems. The process of biodiversity erosion impinges on, and is augmented by, the decimation of local cultural elements, such as food cultures, the vocabularies of local languages, house architecture, and an inchoate appreciation of the non-use value of biodiversity, i.e., beyond its instrumental value. This process of biocultural erosion is evident in the district of Bankura, West Bengal, India, and this article collates evidence from over two decades of my research on the biodiversity and cultural elements of the region. The replacement of a traditional eco-centric ethic with an industrial ethic, and its consequent impacts on biodiversity and local cultural traditions in this region, is illustrative of the global process of biodiversity loss.

Ecological and Evolutionary Consequences of Gene Flow through Pollen from Transgenic Crops to Their Wild Relatives

The rapid progress in biotechnology has significantly promoted the development and production of genetically modified (GM) crops. The extensive global cultivation of GM crops has generated great benefits, which may provide opportunities for solving the problems inherent in world food security, but it has also aroused considerable biosafety concerns worldwide. Among these, the potential environmental consequences created by possible transgene escape from a GM crop to its nonGM crop counterparts and wild or weedy relatives via gene flow are the most debated biosafety issues internationally. Gene flow indicates the movement of genes or genetic materials from one plant population into another. There are a range of predicted possible environmental and evolutionary consequences such as the creation of new weeds, change of fitness-related characters, and loss of genetic diversity in crop landraces and wild relatives) caused by wild transgene flow. In addition, transgene flow also arouses biosafety concerns for food/feed and health and socio-economics and ethics. Through pollenmediated gene flow, a transgene can move from a GM crop into populations of a wild relative, and persist or disseminate in the wild population through further hybridization and introgression between the GM crop and wild relative. If a transgene can express in wild plants as it does in the GM crop, the transgene may change a certain trait of the wild plants, possibly leading to further undesired consequences. If a transgene can alter the fitness of wild plants and the dynamics of the wild populations, the introgression of the transgene in the wild population may cause either local extinction of the population or make the wild population more invasive and competitive. Therefore, a wide variety of possible ecological and evolutionary consequences of gene flow through Pollen from transgenic plants to their wild relatives are discussed on the basis of previous work