In the face of the development of supply chain globalization, Whether it is the choice of goods clearance or transportation mode, the establishment of land waterless port for product transportation, rapid customs clearance, convenient enterprises have a great role. Based on the analysis of the present situation and existing problems of land ports in China, the paper uses improved genetic algorithm to optimize the previous transportation paths. This paper analyzes the present situation and existing problems of waterless ports in China so as to optimize the previous transportation paths by using improved genetic algorithm. Through continuous iterative optimization, the collection paths are shortened and the no-load rate is reduced, so as to make full and reasonable use of transport vehicles, provide better service for enterprises and reduce more costs for waterless ports.