Xinjiang is located in the northwest of China, with a vast territory and abundant resources, which holds an important strategic position for China. However, the climate in the region is arid and the ecological environment is fragile. In recent years, with the growth of population and the improvement of economic development level, construction and agricultural land in Xinjiang have gradually expanded, and ecological land has been extensively occupied. The competition between land resources is becoming increasingly fierce, leading to some ecological and environmental problems in the region. In this context, understanding the spatiotemporal changes and driving factors of Xinjiang's production space, living space and ecological space (PLES) is of great practical significance for promoting sustainable development in the region, and can also provide reference for the research of PLES in other arid areas at home and abroad. At present, most of the research on PLES is focused on small-scale areas such as urban agglomerations, rural areas, and watersheds, or non arid areas with relatively superior natural conditions, with less research on large-scale arid areas. In this study, the transfer matrix, the center of gravity movement trajectory, Moran's I and the spatial conflict measurement model were used to illustrate the spatial–temporal evolution of PLES in Xinjiang, and the correlation coefficient was used to determine the main factors affecting the change of PLES. Results show that (1) Xinjiang is dominated by ecological space, which accounts for more than 90% of the total area of Xinjiang and has significant continuity. They are mainly distributed in Tarim, Turpan, and the northern Junggar Basin. The production space area accounts for about 5% of the total area of Xinjiang and is distributed in the vicinity of the Tianshan and Kunlun Mountains. Living space accounts for about 1% of Xinjiang's total area and is scattered around cities. (2) From 2000 to 2020, the change degree of PLES in Xinjiang in the last decade is higher than that in the first decade, most of which is the transformation from ecological space to production space. The three types of spaces influence and restrict each other in the development process. (3) Population, crop sown area, natural conditions, policy and the development level of agricultural science and technology are the main factors affecting the spatial distribution of production. Environmental factors such as altitude, soil erosion and temperature have a great influence on ecological space. Population, the output value of various industries and total retail sales of consumer goods are the main factors affecting residential space. The evolution of Xinjiang PLES is the result of multiple factors. (4) The medium spatial conflict dominates, mainly distributed in Tarim Basin and Junggar Basin. The weak and strong space conflicts are concentrated in the oases in the north of the Tianshan and Kunlun Mountains, and the number is small. From 2000 to 2020, the increasing degree of spatial conflicts in Xinjiang is due to land desertification.