The lipids of barracuda and longnose lancetfish, which were obtained in the Coral Sea, were examined using GLC, TLC, and GC-MS. Each of the extracted lipids was separated into the following fractions prior to gas chromatographic analyses of their fatty acid compositions: polar lipid (PL), triglyceride (TG), and free fatty acid (FFA). The PL of the barracuda muscle was mainly composed of phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE). The major fatty acids of the PL were C16:0, C18:0, C18:1, and C22:6. The ratio of C18:0/C18:1 in the total lipid of barracuda was 0.5, which is somewhat higher than in ones other marine fish lipids. In longnose lancetfish, the muscle lipids including skin contained an extremely small amount of C18:0. The major constituent fatty acids of PL in longnose lancetfish were C16:0, C18:1, C20:3, and C22:6.