Lamiophlomis Rotata Research Articles

Separation and determination of flavonoids in Lamiophlomis rotata by capillary electrophoresis using borate as electrolyte

A capillary electrophoresis method was developed for the simultaneous determination of five flavonoids such as luteolin-7- O-glucoside, isorhamnetin, apigenin, luteolin and quercetin in Lamiophlomis rotata (Benth.) Kudo. Optimal conditions were obtained at pH 9.0 with 30 mM borate as buffer containing 8% (v/v) acetonitrile, 20 kV as driving voltage and 210 nm as detection wavelength. The association constant K and the change in Gibbs free energy (Δ G) of the interaction of flavonoids with borate anion ion (a typical ion-dipole or ion-induced dipole interaction) were calculated for the quantitative evaluation and characterization of the interaction. The described method was successfully applied for the rapid and efficient quality control by quantifying flavonoids in L. rotata. Repeatability tests showed that the R.S.D.s of both intra- and inter-day migration times and peak areas were less than 5%. The LOD of the five flavonoids was less than 3.75 mg/l. Recovery results ranged from 94.2% to 105.1%.

Soil erosion and vegetation succession in alpine Kobresia steppe meadow caused by plateau pika—A case study of Nagqu County, Tibet

This paper evaluated the impacts of mounds created by the plateau pika (Ochotona curzoniae) on the vegetation composition, structure, and species diversity of an alpine Kobresia steppe meadow in Nagqu County, Tibet Autonomous Region, China. Based on mound height or the depth of erosion pit, we defined five stages of erosion and compared the floristic features of communities at these stages with those in undisturbed sites. In the study area, the mounds and pits covered up to 7% of the total area. Lancea tibetica, Lamiophlomis rotata, and Potentilla bifurca were the dominant species in erosion pits, and Kobresia pygmaea, the dominant species in undisturbed sites, became a companion species in eroded areas. In the process of erosion, the original vegetation was covered by soil ejected by the pika, then the mounds were gradually eroded by wind and rain, and finally erosion pits formed. The vegetation coverage increased with increasing erosion stages but remained significantly lower than that in undisturbed sites. Improved coverage eventually reduced soil erosion, and pit depth eventually stabilized at around 20cm. Aboveground biomass increased with increasing erosion stage, but the proportion of low-quality forage reached more than 94%. The richness index and Shannon-Wiener index increased significantly with increasing erosion stage, but the richness index in mound and pit areas was significantly lower than that in undisturbed sites.

A karyomorphological study in the monotypic genus Lamiophlomis and five species in Phlomis (Lamiaceae)

The monotypic genus Lamiophlomis (including only L. rotata) and five species of Phlomis in the Lamiaceae were karyornorphologically studied. The interphase nuclei are all categorized to be round prochromosome type, and the mitotic-prophase chromosomes are all categorized to be interstitial type. The karyotype formulae are as follows: L. rotata, 2n=2x=22=18m+4sm; R umbrosa, 2n=2x=22=22m; P ruptilis, 2n=2x=22=22m; P betonicoides, 2n=2x=22=22m; P melanantha, 2n=2x=22=22m(2sat); P strigosa, 2n=6x=66= 60m+ 6sm. The basic chromosome number of Lamiophlomis is confirmed to be x=11, the same as that of Phlomis sect. Phlomoides, which shows that Lamiophlomis rotata should be better treated as a species of Phlomis.

Iridoid Glycosides from Lamiophlomis rotata

AbstractThe seven new iridoid diglycosides 1–7, together with eleven known iridoid and phenylpropanoid glycosides, were isolated from the 50% EtOH extract of the roots of Lamiophlomis rotata. Their structures were elucidated by chemical and spectroscopic methods.

Genetic diversity and population structure of Lamiophlomis rotata (Lamiaceae), an endemic species of Qinghai–Tibet Plateau

Lamiophlomis rotata (Lamiaceae), a perennial medicinal herb, is endemic to the Qinghai-Tibet Plateau. A total of 188 individuals from eight natural populations of L. rotata in Qinghai-Tibet Plateau (four from Tibet, two from Yunnan, and two from Qinghai) were analyzed using intersimple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) techniques. Our results revealed that the level of genetic variation in L. rotata was relatively high (P = 94.85%, I = 0.440 +/- 0.220, H(T) = 0.289 +/- 0.028). Three genetic groups corresponding to the three geographic regions were detected, suggesting significant geographic structure. Our results suggest that the highly structured geographic pattern found in L. rotata might represent diverging evolutionary processes associated with the uplifting of the Qinghai-Tibet Plateau and Quaternary climatic oscillations. These findings imply that as many populations as possible should be preserved in situ for the conservation of this species. Given their genetic variability and peripheral distribution, Qinghai and Yunnan populations should be assigned priority for conservation. Optimal harvesting strategies, domestication and tissue culture of L. rotata should be developed as soon as possible to guarantee its sustainable use.

Chemical variation in lipophilic composition of Lamiophlomis rotata from the Qinghai-Tibetan Plateau

Lipophilic extracts from flowers, leaves, and roots of Lamiophlomis rotata (Benth.) were analyzed using GC-MS. A total of 67 compounds were identified.

Phenylpropanoid glycosides from Lamiophlomis rotata

Two new phenypropanoid glycosides were isolated from the roots of Lamiophlomis rotata, together with a known compound, cistanoside C. On the basis of spectral and chemical evidence, the structures of two new compounds were identified as 6'-beta-D-apiofuranosyl cistanoside C and cis-lamiophlomiside A. (C) 1999 Elsevier Science Ltd. All rights reserved.