Associated with Taylor dispersion, in this paper we analyze how the vertical position of a point-source solute release will affect the transport process in laminar open channel flow, through obtaining and applying analytical solution by the two-scale perturbation analysis (Wu and Chen, 2014, J. Fluid Mech., 740, 196–213), which is verified and supported by results from numerical simulations. Based on multi-dimensional spatial concentration distribution of the solute plume, we resort to the previously proposed criterion for identifying the stage of solute transport characterized by the dispersion-dominated (Taylor dispersion) regime, focusing on the relative uniformity of concentration distribution across a given family of curved surfaces (Wu et al., 2016, Sci. Rep., 6, 20556). The most important finding is that for the solute transport transition into the dispersion-dominated regime, the necessary time is about 50% more for the case of solute release at free water surface compared with that at the channel bed, which is substantial under typical physical parameters. Other effects of release position include affecting the displacement of the solute plume centroid, the value of the maximum mean concentration, and non-Gaussian properties regarding the form of the streamwise distribution of the mean concentration.