Lake chlorophyll-a (Chl-a) is one of the important components of the lake ecosystem. Numerous studies have analyzed Chl-a in ocean and inland water ecosystems under pressures from climate change and anthropogenic activities. However, little research has been conducted on lake Chl-a variations in the Tibet Plateau (TP) because of its harsh environment and limited opportunities for in situ data monitoring. Here, we combined 95 in situ measured lake Chl-a concentration data points and the Landsat reflection spectrum to establish an inversion model of Chl-a concentration. For this, we retrieved the mean annual Chl-a concentration in the past 35 years (1986–2021) of 318 lakes with an area of > 10 km2 in the TP using the backpropagation (BP) neural network prediction method. Meteorological and hydrological data, measured water quality parameters, and glacier change in the lake basin, along with geographic information system (GIS) technology and spatial statistical analysis, were used to elucidate the driving factors of the Chl-a concentration changes in the TP lakes. The results showed that the mean annual Chl-a in the 318 lakes displayed an overall decrease during 1986–2021 (−0.03 μg/L/y), but 63%, 32%, and 5% of the total number exhibited no significant change, significant decrease, and significant increase, respectively. After a slight increase during 1986–1995 (0.05 μg/L/y), the mean annual lake Chl-a significantly decreased during 1996–2004 (−0.18 μg/L/y). Further, it decreased slightly during 2005–2021 (−0.02 μg/L/y). The mean annual lake Chl-a concentration was significantly negatively correlated with precipitation (R2 = 0.48, p < 0.01), air temperature (R2 = 0.31, p < 0.01), lake surface water temperature (LSWT) (R2 = 0.51, p < 0.01), lake area (R2 = 0.42, p < 0.01), and lake water volume change (R2 = 0.77, p < 0.01). The Chl-a concentration of non-glacial-meltwater-fed lakes were higher than those of glacial-meltwater-fed lakes, except during higher precipitation periods. Our results shed light on the impacts of climate change on Chl-a variation in the TP lakes and lay the foundation for understanding the changes in the TP lake ecosystem.
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