Lake St Research Articles

Drivers and mechanisms of harmful algal blooms across hydrologic extremes in hypereutrophic grand lake st marys (Ohio)

Grand Lake St. Marys (GLSM) is a large, shallow, hypereutrophic lake situated in an agricultural watershed with high-nutrient, non-point source runoff. The resulting harmful algal blooms (HABs) are typically dominated by Planktothrix, which can produce microcystin, a potent cyanobacterial toxin that has varied in concentration over the past decade. Some drivers of bloom biomass and toxicity in GLSM are described, but recent years (2019–2022) have exhibited anomalous combinations of winter ice cover and spring runoff, suggesting that additional factors contribute to variability in HAB severity and toxicity. 2020 and 2022 were typical water years, with normal tributary runoff volumes occurring primarily in late winter and spring after either little to no ice cover (2019–2020) or heavy/prolonged ice cover (2021–2022). However, 2021 exhibited prolonged winter ice and low winter/spring runoff. 2020 and 2022 were typical bloom years, with near monoculture, Planktothrix-dominated biomass (11 to 405 μg/L total chlorophyll) and high total concentrations of microcystins (<0.3 to 65 μg/L). However, the first half of 2021 exhibited lower biomass (18 to 65 μg/L chlorophyll a) and toxin concentrations (0.4 to 2.0 μg/L). While biomass returned to bloom levels when external tributary loading increased, ammonium uptake and regeneration rates and microcystin concentrations remained low throughout 2021 (in contrast to other years). Overall, potential ammonium uptake rates strongly correlated with chlorophyll and microcystin concentrations (Bayesian R2 = 0.59; 95% CI = 0.44 to 0.65). Phytoplankton diversity was higher in 2021 than other years, especially in spring/early summer, with increased dinoflagellates and diatoms in spring, followed by a mixed cyanobacterial assemblage in summer. These results suggest that lower external nutrient loads can drive immediate positive impacts on water quality, such as reduced HAB biomass and toxicity and higher phytoplankton diversity, even in hypereutrophic, shallow lakes.

The dependence in microcystin removal with powdered activated carbon on variant properties, carbon properties, and dissolved organic matter

Powdered activated carbon (PAC) is commonly used by water treatment plants to remove harmful cyanotoxins such as microcystins (MCs) produced during seasonal harmful algal blooms. MC removal by PAC depends upon the properties of the PAC, the properties of the MC variant, and the presence and properties of dissolved organic matter (DOM). To identify which of these factors has the greatest impact on the removal of MC by PAC, we evaluated the removal of four different MC variants (MC-LR, MC-LA, MC-RR and desmethylated MC-RR) by three different PAC types (wood-based, coal-blend and coal-based). The role of DOM properties was evaluated using DOM isolated from two different sources, a terrestrial source (Suwannee River Fulvic Acid, SRFA) and a microbial source (Grand Lake St Marys DOM, GLSM). The results of adsorption experiments conducted over a period of 72 h demonstrated the wood-based PAC, which had the highest surface area and mesopore volume of the PAC tested, had the highest adsorption rate and capacity for all four MC variants. Of the variants studied, neutrally charged MC-RR was adsorbed more rapidly and to a greater extent on all of the PAC types than were the other variants. Although MC-LA and MC-LR had the greatest hydrophobicity, their negative charges resulted in their being adsorbed the least. As expected, DOM inhibited microcystin adsorption to PAC. The degree of inhibition, however, did not significantly vary for the two DOM types evaluated, indicating the properties of the DOM on MC adsorption to PAC was less important than the PAC properties or MC variant properties. Overall, PAC properties were a more important factor in MC removal than were the MC properties or DOM conditions.

Zooplankton composition following an artificial mouth breaching event in Lake St Lucia, South Africa

Lake St Lucia is one of the most important estuarine systems in South Africa, but the long history of anthropogenic intervention has threatened its integrity. With the exception of sporadic overtopping events and periodic marine connectivity via the uMfolozi River, the St Lucia Estuary remained closed off from the Indian Ocean since 2007. Due to the build-up of sediment and prolonged mouth closure, the beach berm was artificially breached in January 2021, fully reconnecting St Lucia to the Indian Ocean for the first time in 13 years. This study aimed to determine the effect of this breaching event on St Lucia’s zooplankton community by analysing samples from March, May and August 2021, at five representative sites throughout the lake system. The mouth remained open for only five months, and the system was still largely oligo- to meso-haline due to high freshwater input. The patterns in the zooplankton were related more to the prevailing environmental conditions than to the breaching event itself. The composition of zooplankton in the system did change; however, the presence of marine taxa was not far-reaching nor long-lasting, most likely due to the limited tidal intrusion, strong outflow and predominant salinity regime.

Mid-Late Holocene palynological development at Lake St Lucia, KwaZulu-Natal

Palynological studies were done on lacustrine core sediments in the Indian Ocean Coastal Belt Biome of KwaZulu-Natal, eastern South Africa, that were deposited during the last ∼6300 cal yrs BP in the Mkuze River delta that drains into the northern-most part of Lake St Lucia. The aim was to reconstruct the past vegetation and to infer past climate fluctuations as well as human disturbances to complement growing evidence from other disciplines about these aspects in the area. Palynological results show a dominance of Poaceae (20–50%) suggesting the consistent presence of grassy woodland. Podocarpus pollen dominated the arboreal spectra from 6300 to 2600 cal yrs BP. Thereafter Spirostachys pollen dominated the arboreal pollen spectra for the last 2600 cal yrs BP indicating a change from a forested environment to a more open woodland environment caused by a drop in moisture content (humidity). The results also show that the mid Holocene was humid and warm with high precipitation (freshwater conditions) coinciding with raised sea levels. The late Holocene was warm and dry with some marine influence with more seawater flowing into the estuary and lake due to high evaporation and low fresh water supply caused by drought. Similar pollen fluctuations were observed in records from Lakes Eteza and Sibaya, also located within the Indian Ocean Coastal Belt Biome. The presence of Pinus pollen at the top of the profiles suggests the onset of European settlement when pines were introduced for timber production.

Exploring long-term trends in microcystin toxin values associated with persistent harmful algal blooms in Grand Lake St Marys

High external nutrient loads from agricultural runoff have led to persistent and highly toxic algal blooms in Grand Lake St Marys (GLSM) for decades. These pervasive blooms are concurrent with long-term (2009 – 2021) toxin and environmental monitoring, providing a robust weekly dataset for modeling microcystins. Median weekly microcystin concentrations (23.2 µg/L) routinely exceeded World Health Organization recreational limits (20 µg/L) for the study period (ranged 0.03 - 185.0 µg/L). Here, we used a Bayesian hierarchical dynamic linear model to hindcast weekly microcystin toxins using external nutrient loads from tributary data as well as internal lake nutrient and physicochemical concentrations. Overall, lake TN was the biggest driver of microcystin concentration in GLSM. Likewise, TN:TP was a strong negative driver of microcystin (i.e. low N:P ratios align with lower total microcystins), suggesting that N availability directly impacts toxins. External nutrient loading was positively related to microcystin during winter and spring; however, there was no relationship detected between toxin and external loading during summer or fall (particulate phosphorus exhibited the strongest signal but all external nutrients were unsurprisingly correlated). This lack of direct correlation on a weekly timescale between external loads and cyanobacterial toxins during the summer months likely results from nutrient saturation and reflects the importance of internal loading for bloom maintenance as supported by the correlation between in-lake TN and microcystin. Thus, management goals to reduce the highest biomass and toxins in the summer should focus on reduction of winter and spring external nutrient loads. Supporting this, both 2010 and 2021 had lower rain in the first half of the year (winter/spring), resulting in less loading, and experienced smaller/later low toxicity blooms. This suggests that, although internal nutrient loads are important for bloom maintenance, reduced external loads are an effective management strategy even in nutrient saturated systems such as GLSM.

Measuring beachgoer preferences for avoiding harmful algal blooms and bacterial warnings

This paper estimates beachgoers' preferences for beach quality, including avoidance of harmful algal blooms and bacterial warnings. Following a stratified random sampling schedule, data was collected via interviews conducted at 28 public beaches from Eastern Ohio to Northern Lake St Clair. Randomly selected visitors were interviewed and sent a follow-up choice experiment survey, which measured preferences for beach attributes. We find the average respondent is willing to drive 260 and 266 miles to avoid sites with either current HAB or bacterial warnings, and find a negative stigma effect that remains at least 6 days post-warning. While respondents' aversion to active HAB and bacterial warnings are not statistically different, this aversion decreases more slowly after a bacterial warning; respondents are willing to drive 77 miles to avoid a site with a bacterial warning lifted 6 days earlier, but only 31 miles to avoid a site with a HAB warning lifted 6 days earlier. To test our findings' validity, we used the choice model estimates to simulate responses to contingent behavior questions from the follow-up. Although framed differently, the elicitation formats yield concordant findings. Results indicate that cost-benefit analysis which doesn't evaluate the stigma effect of recently-lifted warnings may understate their costs.

Trace Metal Accumulation in Eggs of Wild Nile Crocodiles (Crocodylus niloticus) from Lake St Lucia, South Africa: Implications for Biomonitoring in a Global Biodiversity Hotspot.

Nile crocodiles (Crocodylus niloticus) at Lake St Lucia, South Africa, have some of the highest blood lead (Pb) concentrations ever recorded in wildlife globally. Although exposure to Pb is known to pose major risks to wildlife reproductive success, potential impacts on crocodile reproduction at Lake St Lucia have yet to be examined. In this study, we investigated the accumulation of Pb and other trace metals (Al, V, Cr, Co, Cu, Ni, Zn and Cd) in eggs (n = 20) collected from five wild crocodile nests at Lake St Lucia. All metals analysed in this study were detected in egg contents, although concentrations varied considerably among nests and within clutches. Lead was detected in the contents of all eggs, but only at relatively low concentrations (43 ± 26ngg-1 dry weight). Although sampling limitations commonly associated with wild population surveys prevent a complete assessment of exposure variability, our findings suggest maternal transfer may not be a significant depuration pathway for Pb and females possibly clear Pb through other mechanisms (e.g. sequestration into claws, bone and osteoderms). Metal concentrations in eggshells and shell membranes were poorly correlated with concentrations measured in egg content and thus do not provide viable non-lethal indicators for monitoring metal exposure in Nile crocodiles. Intra-clutch variability accounted for a considerable proportion of the total variance in egg content metal concentrations, suggesting the "one egg" sampling strategy often applied in reptile studies may not be an effective biomonitoring tool for wild crocodilian populations. Although maternally derived Pb does not appear to present widespread toxicological concern at Lake St Lucia, adverse effects of Pb exposure on other reproductive functions (e.g. spermatogenesis) cannot be discounted and warrant further investigation.

High lead exposure and clinical signs of toxicosis in wild Nile crocodiles (Crocodylus niloticus) from a World Heritage site: Lake St Lucia estuarine system, South Africa.

Lead (Pb) exposure is a widespread wildlife conservation threat, but impacts on reptile populations remain poorly documented. In this study, we examined Pb exposure and accumulation in a wild population of Nile crocodiles (Crocodylus niloticus) at Lake St Lucia, South Africa. Recreational angling has occurred in the area since the 1930s and incidental ingestion of Pb fishing weights has previously been identified as a major source of Pb poisoning in the local crocodile population. In 2019, we sampled blood and tail fat tissues from wild (n=22) and captive (n=3) crocodiles at Lake St Lucia to investigate potential impacts of chronic Pb exposure on crocodilian health. Lead was detected in blood samples of all wild crocodiles, although concentrations varied widely between individuals (86-13,100ngml-1). The incidence of Pb poisoning was higher in male crocodiles, with mean blood lead (BPb) concentrations in males (3780±4690ngml-1) significantly (p<0.001) higher compared to females (266±230ngml-1). Blood Pb concentrations were correlated with concentrations measured in tail fat tissue (n.d - 4175ngg-1 wet wt.). Although most of the crocodiles sampled appeared to be in good physical condition, highly elevated BPb concentrations (>6000ngml-1) were associated with markedly suppressed packed cell volumes (4.6-10.8%) and severe deterioration in tooth condition. These findings suggest that anaemia and tooth loss may be clinical signs of long-term environmental exposure to Pb. Although previously undocumented in crocodilians, these symptoms are consistent with Pb poisoning observed in birds and mammals, and suggest that crocodilians may be more susceptible to the long-term toxic effects of Pb than previously thought. In light of these findings, we suggest that the impact of accumulated Pb on crocodilian fitness, reproduction and mortality requires urgent attention.

Drone-based water sampling and characterization of three freshwater harmful algal blooms in the United States

Freshwater harmful algal blooms (HABs), caused mostly by toxic cyanobacteria, produce a range of cyanotoxins that threaten the health of humans and domestic animals. Climate conditions and anthropogenic influences such as agricultural run-off can alter the onset and intensity of HABs. Little is known about the distribution and spread of freshwater HABs. Current sampling protocols in some lakes involve teams of researchers that collect samples by hand from a boat and/or from the shoreline. Water samples can be collected from the surface, from discrete-depth collections, and/or from depth-integrated intervals. These collections are often restricted to certain months of the year, and generally are only performed at a limited number of collection sites. In lakes with active HABs, surface samples are generally sufficient for HAB water quality assessments. We used a unique DrOne Water Sampling SystEm (DOWSE) to collect water samples from the surface of three different HABs in Ohio (Grand Lake St Marys, GLSM and Lake Erie) and Virginia (Lake Anna), United States in 2019. The DOWSE consisted of a 3D-printed sampling device tethered to a drone (uncrewed aerial system, or UAS), and was used to collect surface water samples at different distances (10–100 m) from the shore or from an anchored boat. One hundred and eighty water samples (40 at GLSM, 20 at Lake Erie, and 120 at Lake Anna) were collected and analyzed from 18 drone flights. Our methods included testing for cyanotoxins, phycocyanin, and nutrients from surface water samples. Mean concentrations of microcystins (MCs) in drone water samples were 15.00, 1.92, and 0.02 ppb for GLSM, Lake Erie, and Lake Anna, respectively. Lake Anna had low levels of anatoxin in nearly all (111/120) of the drone water samples. Mean concentrations of phycocyanin in drone water samples were 687, 38, and 62 ppb for GLSM, Lake Erie, and Lake Anna, respectively. High levels of total phosphorus were observed in the drone water samples from GLSM (mean of 0.34 mg/L) and Lake Erie (mean of 0.12 mg/L). Lake Anna had the highest variability of total phosphorus with concentrations that ranged from 0.01 mg/L to 0.21 mg/L, with a mean of 0.06 mg/L. Nitrate levels varied greatly across sites, inverse with bloom biomass, ranging from below detection to 3.64 mg/L, with highest mean values in Lake Erie followed by GLSM and Lake Anna, respectively. Drones offer a rapid, targeted collection of water samples from virtually anywhere on a lake with an active HAB without the need for a boat which can disturb the surrounding water. Drones are, however, limited in their ability to operate during inclement weather such as rain and heavy winds. Collectively, our results highlight numerous opportunities for drone-based water sampling technologies to track, predict, and respond to HABs in the future.

Origin, transport, and retention of fluvial sedimentary organic matter in South Africa's largest freshwater wetland, Mkhuze Wetland System

Abstract. Sedimentary organic matter (OM) analyses along a 130 km long transect of the Mkhuze River from the Lebombo Mountains to its outlet into Lake St Lucia, Africa's most extensive estuarine system, revealed the present active trapping function of a terminal freshwater wetland. Combining bulk OM analyses, such as Rock-Eval®, and source-specific biomarker analyses of plant-wax n-alkanes and their stable carbon (δ13C) and hydrogen (δD) isotopic composition showed that fluvial sedimentary OM originating from inland areas is mainly deposited in the floodplain and swamp area of the wetland system but not in the downstream lake area. A distinctly less degraded OM signature, i.e., a considerably lower degree of transformation of unstable components (higher I index) and lower contribution of refractory and persistent fractions (lower R index) as well as recognizably higher δD values compared to samples from upstream sub-environments, characterizes surface sediments of Lake St Lucia. The offset in δD indicates that the contributing vegetation, although similar to upstream vegetation inputs in terms of photosynthetic pathway (δ13C) and alkane distribution pattern, experienced different hydrological growth conditions. The results suggest that under current conditions hinterland sedimentary OM is deposited throughout the wetland system up to the Mkhuze Swamps, which ultimately captures the transported OM. Consequently, samples from the downstream located Lake St Lucia show locally derived signals instead of integrated signals encompassing the river catchment. This finding raises important constraints for future environmental studies as the assumption of watershed-integrated signals in sedimentary archives retrieved from downstream lakes or offshore might not hold true in certain settings.

The Holocene evolution of Lake St Lucia, Africa's largest estuary: Geological implications for contemporary management

The Holocene evolution of one of Africa's largest estuaries, Lake St Lucia on the east coast of South Africa, is examined and juxtaposed with previous and contemporary management practices aimed at fixing the estuary state at a single point along the system's overall evolutionary pathway. The estuary has been heavily altered over the course of the last century, mostly through mouth management activities aimed at maintaining a direct connection with the ocean. Based on a high-resolution seismic reflection dataset, LiDAR and bathymetry data of the lake system, together with palaeontological (foraminifera and diatoms) and sulfur isotope geochemical data from three long (14–16 m) piston cores, we investigate the system-wide and coeval evolution of the three different sub-basins (False Bay, North Lake and South Lake) that comprise the current estuarine lake system. The northern and southern sections of the system evolved independently of each other since the Last Glacial Maximum, with each area responding differently to early sea-level forcings. In North Lake, the lagoon evolved from fully estuarine (8300 cal. BP) to a restricted lagoon with occasional marine incursions, steadily decreasing tidal prism and the development of marginal spits by wind-driven lagoonal waves. The tidal inlet closed approximately 6200 cal. BP and fluvial conditions became more dominant as main back-barrier conditions became attuned to fluvial supply. Similar conditions prevailed in the adjacent False Bay basin, albeit with an earlier onset due to sheltering by prominent bedrock peninsulas. In contrast, South Lake evolved from a fully estuarine system (9500 cal. BP) with a persistent ocean connection, to a segmented system with a diminished tidal connection by 5500 cal. BP. The inlet sealed at this point and an isolated back barrier system developed. This prompted the system-wide impoundment of waters causing back-barrier flooding and in situ drowning of bayhead deltas and segmenting spits. By 2000 cal. BP, these areas had flooded sufficiently to connect over a bedrock high that had diverted the initial palaeo-valleys to the north and south. A new, single lagoon, representative of the modern-day system formed, with a new marine connection to the modern day Mfolozi River and contemporary estuary. Periodic desiccation of the lake sub-basins since this point has been apparent. Given the complex management plans implemented to stop excess sedimentation, hypersaline events, and the continuation of “normal” lake levels, this is at odds with the natural system's evolution to a pan or wetland-type state. This geological background should be key to informing management plans that work within the natural evolutionary trajectory of the system, as opposed to attempting to maintain an unattainable status quo.

High Lead Exposure and Clinical Signs of Toxicosis in Wild Nile Crocodiles (Crocodylus Niloticus) from a World Heritage Site: Lake St Lucia Estuarine System, South Africa

High Lead Exposure and Clinical Signs of Toxicosis in Wild Nile Crocodiles (Crocodylus Niloticus) from a World Heritage Site: Lake St Lucia Estuarine System, South Africa

Bioaccumulation of current-use herbicides in fish from a global biodiversity hotspot: Lake St Lucia, South Africa

Agricultural pesticides threaten aquatic systems and biodiversity at a global scale, but limited information is available on the accumulation of current-use herbicides in tissues of aquatic organisms. Here, we examine the potential exposure and accumulation of currently used herbicides in two species of fish from Lake St Lucia, a global biodiversity hotspot located in South Africa. Muscle tissue samples were analysed for 11 widely used multi-residue and phenoxy-acid herbicides. Herbicide residues were detected in all samples analysed, with total concentrations ranging from 44.3 to 238 ng g−1 (Clarias gariepinus) and 72.2–291 ng g−1 dw (Oreochromis mossambicus). The most prominent herbicides detected included the two phenoxy-acid herbicides, MCPA (17.6 ± 12 ng g−1) and 2,4-D (28.9 ± 16 ng g−1), along with acetochlor (15.4 ± 5.8 ng g−1), atrazine (12.7 ± 7.1 ng g−1) and terbuthylazine (12.4 ± 12 ng g−1). Results indicate that fish at Lake St Lucia accumulate a complex mixture of herbicides, some previously unreported in tissue, highlighting the potential threat that agricultural runoff may pose to conservation areas. However, assessing the impact of herbicide accumulation on wild fish populations is difficult at present and urgent toxicological data are needed to better understand chronic exposure effects on aquatic organisms. A preliminary human health risk assessment indicated minimal risk associated with the consumption of local fish, but potential additive and synergistic effects of contaminant mixtures remain unknown.

Spatial trends and temporal declines in tissue metals/metalloids in the context of wild fish health at the St. Clair River Area of Concern

The St. Clair River, which flows from Lake Huron to Lake St Clair, receives discharges and emissions from numerous petroleum refineries, petrochemical facilities, and other sources. We used a multi-factor ANOVA to test for spatial and temporal changes in concentrations of trace metals/metalloids in homogenates of fish from Lake Huron (reference site) and two river sites - an industrialized region (Stag Island) and downstream Walpole Island/Chenal Écarte. In 2002/2003 and in 2014, we sampled 20 adult fish of each sex of the following species: shorthead redhorse (Moxostoma macrolepidotum), yellow perch (Perca flavescens); we added emerald shiner (Notropis atherinoides; unsexed) in 2014. There was a temporal decline of most metals/metalloids in shorthead redhorse at the river sites. Linear Discriminant Analysis separated the 2014 shorthead redhorse by site. Emerald shiners had higher concentrations of metals/metalloids, apart from mercury, than the other species; those concentrations were highest at Chenal Écarte, which warrants further research. Tissue concentrations of mercury, which declined temporally at Stag Island and Walpole Island, were above the protective guidance for wildlife consumers at all sites, but were lower than predicted to affect fish health. Apart from increased liver size (ANCOVA) in shorthead redhorse at Walpole Island (2014), the health variables in the riverine fish were stable or differed slightly from reference, which indicated good health and reproductive potential. In shorthead redhorse, tissue metals correlated inconsistently with health indices. Further research could ascertain if temporal declines in metals/metalloids in riverine SHRH are benefits of remediation actions or fluctuations with other causes.

Organochlorine pesticide accumulation in fish and catchment sediments of Lake St Lucia: Risks for Africa’s largest estuary

Although use of organochlorine pesticides (OCPs) has been banned or severely limited on a global basis, concerning concentrations continue to be reported in many tropical and subtropical regions of the world. These habitats often support high levels of unique biodiversity and vulnerable communities that depend on the environment for their survival. We investigated threats associated with OCP contamination at Lake St Lucia, a global hotspot for biodiversity and a UNESCO World Heritage Site in South Africa. Lake St Lucia is sustained largely by surface runoff from catchment areas where significant quantities of OCPs have historically been used in agriculture and where DDT continues to be used for disease control. Sediments (n = 40) collected from the two largest fluvial inputs to Lake St Lucia showed that these rivers represent important sources of contaminants, with ∑OCP concentrations ranging between 74 and 510 ng g−1. Measured HCH, dieldrin, ∑DDT and ∑chlor concentrations exceeded NOAA sediment toxicity guidelines in the majority of samples analysed. Bioaccumulation was assessed by examining residue concentrations in muscle tissues from two abundant fish species from Lake St Lucia. OCPs were detected in all samples analysed, with total concentrations ranging 860–5000 ng g−1 lw and 390–3200 ng g−1 lw for Oreochromis mossambicus (n = 17) and Clarias gariepinus (n = 41), respectively. A health risk assessment indicated potential dietary risk associated with exposure to aldrin, dieldrin and heptachlor, although the cumulative effect of OCPs on human health, ecosystem biodiversity and long-term ecotourism sustainability remains unknown.

Hitchhiking mites

Phoresy is an association between two species in which one transports another. Burying beetles (Nicrophorus spp), also known as carrion beetles, carry mites. There are about 20 phoretic mites on this Nicrophorus beetle, seen in the Great Lakes–St Lawrence forest near Gravenhurst, Canada, and these mites will remain attached until they arrive at the beetle's next carcass. Male Nicrophorus beetles first locate the carcasses of small mammals or birds, and then prepare them by stripping off the fur or feathers. After locating a suitable male and mating, the female lays her eggs inside the prepared carrion. Males and females then bury the carcass in a ~20-cm-deep grave (which will become the beetle's brood chamber) by gradually excavating soil beneath it and covering it with antimicrobial secretions. Burying beetles are unusual in that both males and females take part in raising their young. The American burying beetle (Nicrophorus americanus) historically inhabited 35 states and three Canadian provinces but is now found in only five states and one province. In 1989, the US Fish and Wildlife Service listed it as endangered.

Herbicide residues in sediments from Lake St Lucia (iSimangaliso World Heritage Site, South Africa) and its catchment areas: Occurrence and ecological risk assessment

The impact of agricultural pesticides on sensitive aquatic ecosystems is a matter of global concern. Although South Africa is the largest user of pesticides in sub-Saharan Africa, few studies have examined the toxicological threats posed by agricultural runoff, particularly to conservation areas of international importance. This study investigated the occurrence of 11 priority listed herbicides in sediments from Lake St Lucia, located on the east coast of South Africa. While characterised by exceptionally high levels of biodiversity, Lake St Lucia is affected by agricultural runoff primarily via inflow from two major rivers; the Mkhuze and Mfolozi. Sediment samples collected from Lake St Lucia and its two major fluvial inputs reveal widespread herbicide contamination of the aquatic environment. Residues were detected in the vast majority of samples analysed, with Mkhuze (27.3 ± 17 ng g−1) and Mfolozi (25.6 ± 20 ng g−1) sediments characterised by similar total herbicide levels, while lower concentrations were typically detected in Lake St Lucia (12.9 ± 12 ng g−1). Overall, the most prominent residues detected included acetochlor (3.77 ± 1.3 ng g−1), hexazinone (2.86 ± 1.4 ng g−1) and metolachlor (10.1 ± 8.7 ng g−1). Ecological assessment using Risk Quotients (RQs) showed that cumulative values for triazines and anilides/aniline herbicide classes presented low to medium risk for algae and aquatic invertebrate communities. Considering the biological importance of Lake St Lucia as a nursery for aquatic organisms, it is recommended that further research on the aquatic health of the system be undertaken. Additional monitoring and investigation into mitigation strategies is suggested, particularly as agricultural activities surrounding Lake St Lucia are likely to expand in the future.

Organochlorine pesticide bioaccumulation in wild Nile crocodile (Crocodylus niloticus) fat tissues: Environmental influences on changing residue levels and contaminant profiles

Biologically significant concentrations of organochlorine pesticides (OCPs) continue to be reported in wildlife populations and are of particular concern in species that occupy the highest trophic levels. Nile crocodiles (Crocodylus niloticus) are important apex predators occurring throughout much of tropical and subtropical sub-Saharan Africa, where they inhabit estuarine and freshwater habitats often impacted by contamination. In this study we examined pesticide residue accumulation in fat tissue from Nile crocodiles at Lake St Lucia, South Africa, where historically large quantities of OCPs have been used for agriculture and disease control. During 2019, we collected tail fat samples from wild (n = 21) and captive (n = 3) individuals to examine the influence of habitat, body size and sex on variations in bioaccumulation. The principal contaminant found was p,p′-DDE, a major persistent metabolite of DDT, which continues to be used in the region for combating malaria. Tissue p,p′-DDE concentrations in wild crocodiles (95–1200 ng g−1 ww) were significantly (p < 0.05) higher compared to captive individuals (23–68 ng g−1 ww) and strongly correlated (R2 > 0.70) to body length. Male (n = 14) and female (n = 7) wild crocodiles exhibited similar contaminant body burdens, however, total concentrations were substantially lower than those measured in the same population during 2016/2017. Marked differences in residue levels and profiles appear to reflect changes in food availability and dietary exposure associated with a shift in environmental conditions. These findings suggest that periods of environmental stress may be associated with enhanced toxicological risk in crocodiles. Additional work is needed to better understand contaminant accumulation and elimination mechanisms in crocodiles, and their potential effects on reproductive health.

Changes in the fish community of the St Lucia estuarine system (South Africa) following Cyclone Gamede, an episodic cyclonic event

Lake St Lucia, the largest estuarine lake system in Africa, has been closed since 2002 except for a five month period in 2007 when the mouth was breached by an episodic event, Cyclone Gamede. Mouth opening in 2007 was preceded by five years of low lake levels and hypersaline conditions, which changed the fish species composition and greatly reduced marine fish species diversity. Mouth opening in March 2007 caused the system to partially fill and marine salinities were restored, allowing recruitment of marine species. This study assessed changes in the fish community during the period 2008–2011 following mouth closure after the Cyclone Gamede breaching event. A total of 19,726 fish representing 61 species were collected using seine netting at six sites across the system during biannual sampling between 2008 and 2011. After mouth closure in August 2007, salinities increased and North Lake became hypersaline during 2009–2010. Marine species initially dominated in terms of species number, but freshwater and estuarine species became progressively more abundant, notably Oreochromis mossambicus and Ambassis ambassis. The number of species decreased from 51 in 2008 to 37 in 2011, with 22 marine and two estuarine species being lost from the system. The abundance of marine species decreased by >75% as the system became progressively more saline and the closed mouth limited recruitment to infrequent overtopping events. Changes in the fish community were largely driven by salinity changes across the three main regions of the system. Although management efforts to increase freshwater inflow from the Mfolozi estuary was successful, rapid siltation of the mouth area is a concern.

The influence of prolonged drought and anthropogenic impact reversal on fish community structure and ecosystem state cycling of Lake St Lucia, Africa’s largest estuarine lake

Historically, the St Lucia Estuarine Lake System and uMfolozi River joined via an estuarine bay before exiting to the sea. In 1952, separate mouths were created, because of sediment problems originating from the uMfolozi River. During the 2004 drought, the mouth of the 525 km2 St Lucia System closed and has remained so to date, except for a single opening in 2007, lasting six months. From 2008, management actions have been implemented to 'reconnect' St Lucia to uMfolozi. This study assessed the effects of a new channel between the two on the St Lucia fish community and its effectiveness for recruitment of marine species. Twelve marine species previously absent and a 67% increase in abundance in the Lower Narrows were recorded after channel opening, which allowed for limited recruitment of marine species. This paper reviews the effects of a permanent connection between St Lucia and uMfolozi, via the uMfolozi Spillway, established in 2012, which has pushed the system into a prolonged freshwater state severely reducing the estuarine fish fauna. This, the lack of a connection to the sea for 15 years and issues potentially associated with increased sedimentation in the lower reaches are reviewed in relation to management decisions that have been implemented.