Lake Annecy Research Articles

Holocene Paleohydrological Changes Reflected in Lake-Level Fluctuations in Lake Annecy (French Pre-Alps): Climatic Significance and Archeological Implications

Lakes are threatened by contemporary climate change and human activities. Paleohydrological records provide important evidence for developing scenarios for future changes in the availability of freshwater resources. This study presents a synthesis of a sedimentological, archeological, and chronological dataset collected from Lake Annecy (eastern France) to reconstruct a lake-level record documenting the whole Holocene. This dataset shows a pronounced minimum in the lake level during the Holocene thermal maximum (HTM) (ca. 9000–7000 cal BP), preceded by a general lowering trend (early Holocene), and followed by a general rising trend (Neoglacial). On both the millennial and centennial scales, the Lake Annecy record appears to match the regional pattern of Holocene lake-level fluctuations established for West-Central Europe. In agreement with other extra-regional paleoclimatic records, it shows the dominant influence of orbital forcing. The high magnitude of the lake-level lowering (more than 5 m) during the HTM, with a 2–2.5 °C difference between the HTM and the pre-industrial mean summer temperatures, suggests possible drastic lake-level lowering phases in the near future depending on the IPCC scenarios following climate change. This would mean dramatic impacts on human activities and the preservation of exceptional archeological remains in regional lake basins.

Impact of fishing activities on the population dynamics of European whitefish in four peri-alpine lakes

Inland fisheries play a crucial role in socio-economic activities and food production. However, these fisheries are often less intensively monitored and studied than marine fisheries. In the past two decades, important fluctuations in European whitefish (Coregonus lavaretus) landings have been observed in four peri-alpine lakes in France and Switzerland. To better gauge the impact of fishing removal, a multi-lakes Delay-Difference model was developed and adapted to these fisheries. A hierarchical state-space Bayesian framework was used to integrate stochasticity for each process and incorporate diverse data. The model gave estimates of time series for biomass, recruitment, stage-structured landings, and fishing mortality, as well as estimates of natural mortality rate, Brody growth coefficient and mean recruitment productivity. Differences in the estimates of variables predicted by the model were observed among lakes. Negative impacts of exploitation have been highlighted for Lakes Geneva, Bourget, and Annecy. Low levels of biomass under high fishing mortality rates were observed, while landings tended to increase under lower mortality rates. Lakes Geneva and Bourget outputs suggest stocks were impacted by fishing activities and that fishing effort reduction could ultimately increase yields. Lake Annecy abundance and landings trends increased after 2010, suggesting management strategies that focused on effort reduction were successful. Lake Neuchâtel stock abundance was low after 2017 and probably more fragile due to low biomass levels and recruitment. The observed changes in European whitefish biomass cannot be attributed solely to fishing, suggesting that environmental factors may also influence recruitment success alongside the impacts of fishing pressure.

Winter is not coming: evaluating impacts of changing winter conditions on coregonine reproductive phenology

Fishes in northern latitude lakes are at risk from climate-induced warming because the seasonality in water temperature is degrading, which can change ecosystem properties and the phenology of life-history events. Temperature-dependent embryo development models were developed for a group of cold, stenothermic fishes (Salmonidae Coregoninae) to assess the potential impacts of climate-induced changes in water temperature on cisco (Coregonus artedi) from two populations in Lake Superior (Apostle Islands [USA] and Thunder Bay [Canada]) and one in Lake Ontario (USA), vendace (C. albula) in Lake Southern Konnevesi (Finland), and European whitefish (C. lavaretus) in lakes Southern Konnevesi, Constance (Germany), Geneva (France), and Annecy (France). Water temperatures for each study group were simulated and changes in reproductive phenology across historic (1900–2006) and three future climatic-warming scenarios (2007–2099) were investigated. Models predicted that increases in water temperatures are likely to cause delayed spawning, shorter embryo incubation durations, and earlier larval hatching. Relative changes increased as warming scenarios increased in severity and were higher for littoral as compared to pelagic populations. Our simulations demonstrated that slower cooling in the autumn and (or) more rapid warming in spring can translate into substantial changes in the reproductive phenology of coregonines among our study groups. We expect that the changes in reproductive phenology predicted by our models, in the absence of thermal or behavioral adaptation, will have negative implications for population sustainability.

Synchrony in whitefish stock dynamics: disentangling the effects of local drivers and climate

Synchronic variations in abundance in populations of the same species are common phenomena encountered in various environments, including lakes, and different taxa of freshwater fishes. This phenomenon can be caused by similar environmental conditions across physically separated populations. In the context of the ongoing climate change, it is essential to test this hypothesis, identify the factors driving the synchrony and elucidate the mechanisms, in the attempt to improve fisheries management. This study investigates synchronic variations in European whitefish (Coregonus spp.) populations in five peri-alpine lakes. The hypothesis suggests that shared biotic or abiotic factors contribute to similar trends in whitefish landings. Environmental and seasonal variables impacting the early life stages of the species were analyzed, and the Euclidean distances between the multivariate time series were calculated to identify similarities or dissimilarities in lake environmental parameters. We found that regional winter and spring temperatures were consistent across the lakes, but these factors did not fully account for variations in landings statistics. Wind intensity, water level and zooplankton abundance showed lake-specific patterns that could better explain local conditions and dynamics. Linear models did not reveal a coherent correlation with a common environmental variable across all lakes. However, distinct relationships were found in four of the lakes, with local factors significantly contributing to abundance variations. The spring abundance of Daphnia spp., a primary food source for whitefish larvae, was the main factor correlated with fish landing trends in Lake Geneva and Lake Bourget. Higher availability of Daphnia spp. may decrease intraspecific competition and density-dependent mortality. In Lake Neuchâtel, winter water temperature was negatively correlated with fish abundance proxies, suggesting that warmer winters may compromise reproduction success. Lake Annecy saw an increase in whitefish landings following a substantial reduction in fishing efforts during the late 2000s. A significant negative correlation was found between whitefish landings and fishing efforts. No relationship was found for Lake Aiguebelette, maybe due to a lack of zooplankton data. In conclusion, the observed synchrony in the European whitefish population is likely driven by a combination of interacting environmental and anthropogenic factors rather than a single common variable. Further research and a more detailed dataset are needed to better understand these complex relationships. Cover image: Whitefish (courtesy of Rémi Masson)

Lidar Profiling of Aerosol Vertical Distribution in the Urbanized French Alpine Valley of Annecy and Impact of a Saharan Dust Transport Event

The vertical aerosol layering of the troposphere is poorly documented in mountainous regions, particularly in the Alpine valleys, which are influenced by valley and mountain winds. To improve our knowledge of particulate matter trapped in the Annecy valley, synergetic measurements performed by a ground-based meteorological Raman lidar and a Rayleigh-Mie lidar aboard an ultralight aircraft were implemented as part of the Lacustrine-Water vApor Isotope inVentory Experiment (L-WAIVE) over Lake Annecy. These observations were complemented by satellite observations and Lagrangian modeling. The vertical profiles of aerosol optical properties (e.g., aerosol extinction coefficient (AEC), lidar ratio (LR), particle linear depolarization ratio (PDR)) are derived from lidar measurements at 355 nm during the period between 13 and 22 June 2019. The background aerosol content with an aerosol optical thickness (AOT) of 0.10 ± 0.05, corresponding to local–regional conditions influenced by anthropogenic pollution, has been characterized over the entirety of Lake Annecy thanks to the mobile ultralight payload. The aerosol optical properties are shown to be particularly variable over time in the atmospheric column, with mean LRs (PDRs) varying between 40 ± 8 and 115 ± 15 sr (2 ± 1 and 35 ± 2%). Those conditions can be disturbed by air masses that have recirculated over the valley, as well as by contributions from neighboring valleys. We have observed an important disruption in the atmospheric aerosol profiles by the arrival of an exceptionally dry air mass (RH ~ 30%), containing aerosols identified as coming from the Great Western Erg (AOT ~ 0.5, LR = 65 ± 10 sr, PDR = 20–35%) in the Sahara. These desert dust particles are shown to influence the entire atmospheric column in the Annecy valley. Such an experimental approach, coupling upward and downward lidar and spaceborne observation/Lagrangian modelling, was shown to be of significant interest for the long-term monitoring of the evolution of aerosol loads over deep valleys. It allows a better understanding of the influence of dust storms in the presence of severe convective weather processes.

Did the Younger Dryas to Holocene climate transition favour high seismicity rates in the north‐western Alps?

ABSTRACTIn the French north‐western Alps, several lakes of glacial origin, such as Aiguebelette and La Thuile, present some mass‐transport deposits within their sedimentary sequences. These event layers can result from lake sediment destabilization eventually triggered by earthquakes. On Lake Aiguebelette, based on sedimentological, geochemical and magnetic analyses, and high‐resolution seismic and bathymetric surveys a ca 1 m thick event layer was identified in the deepest lake basin and a synchronous ca 2 cm thick deposit in the shallow basin. Age‐depth models based on radiocarbon ages reveal that both mass‐transport deposits in Lake Aiguebelette occurred around the Younger Dryas – Early Holocene climatic transition (i.e. 11 700 cal bp) time range. In Lake La Thuile, located 30 km away, unique mass‐transport deposits (translational slide type) were recorded at the same time range in sedimentary records. Additionally, high‐resolution seismic profiles previously acquired in Lake Annecy and Lake Bourget support the hypothesis of significant mass‐transport deposits occurring at the Younger Dryas – Early Holocene time range. These outcomes on four north‐western peri‐Alpine and Alpine lakes highlight the regional occurrence of mass‐transport deposits in the Younger Dryas – Early Holocene time range. Seismic and rockfall events are discussed as potential sources of these significant and similarly aged mass‐transport deposits. Based on this study and a literature review, the authors suggest that mechanisms induced by rapid climate change and glacial retreat, such as crustal rebound and erosional unloading, could favour the triggering of earthquakes and rockfall events. In the case of mass‐transport deposits archived in north‐western Alpine lakes during this time period, this study favours the hypothesis of increased seismicity as the primary source driving process involved.

A Comparative Study of the Dynamics and Diversity of Bdellovibrio and Like Organisms in Lakes Annecy and Geneva.

Bdellovibrio and like organisms (BALOs) are obligate bacterial predators of other Gram-negative bacteria. Here, we used quantitative PCR (qPCR) and recently developed specific primers which target the 16S rRNA gene to explore the abundance and distribution of three families of BALO belonging to the Oligoflexia class (i.e., Bdellovibrionaceae, Peredibacteraceae and Bacteriovoracaceae) over one year in the epilimnion and hypolimnion of Lakes Annecy and Geneva. Peredibacteraceae was the dominant group at all sampling points except at the bottom of Lake Geneva, where Bdellovibrionaceae was found in higher number. In addition, the abundance of BALOs increased significantly during the warmer months. Using high-throughput sequencing (Illumina Miseq), hundreds of OTUs were identified for Bdellovibrionaceae and Peredibacteraceae. Phylogenetic analysis suggests that Bdellovibrionaceae are more diverse than Peredibacteraceae and that some OTUs belong to new species of Bdellovibrionaceae. We also found that dominant OTUs were present simultaneously in the two lakes, while some others were specific to each lake, suggesting an adaptive pattern. Finally, both abundance and diversity of BALOs were poorly associated with abiotic factors except temperature, suggesting the importance of studying biotic relationships, assumed to play a greater role than physico-chemical variables in BALOs’ dynamics and distribution.

Effect of a Transducer Horizontality Default on Lake Fish Stock Assessment

Freshwater hydroacoustic surveys are frequently performed with small research boats. Thus, the transducer, usually fixed on a pole on the side of the boat, could be misaligned with the horizontal axis (i.e., the lake surface). Given that fish acoustic responses are strongly directional, the transducer angle could induce attenuation of their backscattering strengths. To test the influence of a small shift of the transducer orientation, we compared hydroacoustics estimates from two sampling nights on the Lake Annecy: the first one being a transducer angle of $$1.5^{\circ }$$ with the horizontal and the second one being strictly horizontal. We showed that the effect of such an angle is negligible over the sampling variability on the TS distribution of the fish population and the biomass assessment. We conclude that a small shift in the transducer horizontality ( $$\le 1.5^{\circ }$$ ) will not degrade the quality of the acoustic data.

Two new Punctastriata (Bacillariophyta) species from subalpine French lakes

ABSTRACT Two new diatom species are described from France. Both species have characters that allow them to be included in the genus Punctastriata D.M.Williams & Round whose main defining character is the “multiseriate striae” (i.e. presence of slender vimines and viminules). Punctastriata subalpina sp. nov. from Lake Annecy has lanceolate valve outline, with slightly heteropolar valves, wide central sternum and Y-bifurcated spines located on the virgae. Some distinguishing features observable in light microscopy (compared to other known small elliptical species in the genus), are the relatively larger cells and the wider distinct sternum. Punctastriata catenata sp. nov. from Lake Entonnoir is similar (under light microscopy) to Staurosirella mutabilis (W.Smith) E.Morales & Van de Vijver and Staurosirella neopinnata E.Morales, C.E.Wetzel, E.Y.Haworth & Ector [= Staurosirella pinnata (Ehrenberg) sensu auct. nonnull]. Punctastriata catenata is characterized by its elongated and elliptic valves, mostly isopolar. The cells have rounded apices and a straight and narrow sternum, and long and strongly silicified spatulated spines rising from the virgae allowing the formation of filaments.

Major biomass fluctuations in lake food webs – An example in the peri-alpine Lake Annecy

In aquatic ecosystems, young of the year (YOY) fish often exhibit strong interannual fluctuations. Because these fish prey on zooplankton and are preyed upon by piscivorous fish, strong fluctuations in their abundance may have important impacts on food web functioning. The static Ecopath model was used to assess potential impacts of strong fluctuations in YOY perch in the deep peri-alpine Lake Annecy. We modeled two contrasting years in terms of YOY perch biomass: a situation with a high biomass of YOY perch and a situation with a low biomass of YOY perch. Additionally, five models were derived from the two initial models to better explore the effects of YOY perch biomass fluctuations on food web functioning. Disparities were revealed in terms of the activity of the system (volume of flows) and the trophic transfer efficiency. When the YOY perch biomass was high, the volume of flows within the system was less important, but the trophic transfers were more efficient than when the biomass was low. The high biomass of YOY perch appeared to facilitate transfers from low to high trophic levels. The results indicated that strong variabilities in the abundance of YOY fish had little impact on the main food web flows and pathways because of the capacity of predators to feed on various prey (omnivory) but could induce significant differences in food web properties (e.g. ascendancy, robustness) and organization (e.g. cycling, mean trophic level).

Including 38 kHz in the standardization protocol for hydroacoustic fish surveys in temperate lakes

AbstractHydroacoustics has become a requisite method to assess fish populations and allows to describe the relationships of fish with other elements of the aquatic ecosystem. This nonintrusive method is currently an integral part of the sampling procedures recommended for fish stock assessment by the Water Framework Directive and has been standardized by the European Committee for Standardization [CEN (2014) CSN EN 15910 ‐ Water quality ‐ Guidance on the estimation of fish abundance with mobile hydroacoustic methods, Category: 7577 Water quality. Biological.]. In Europe, hydroacoustic surveys are performed in freshwater using different frequencies. Consequently, there is a need to evaluate if survey results can be compared. This study aimed to carry out in situ comparisons at the 38 kHz frequency (noted f) with two other commonly used frequencies, 70 and 200 kHz. The 38 kHz frequency has seldom been compared with other frequencies in freshwater although it is widely used worldwide, especially in the Great Lakes of North America and in Sweden. In 2016, hydroacoustic data were acquired in Lakes Annecy and Bourget using methods validated in previous studies that compared the frequencies 70, 120 and 200 kHz. This study showed similar density and biomass estimations as a function of frequency, density(f) and biomass(f), between the frequencies studied for low to moderate fish densities. For higher fish densities, the results were more variable and need to be verified. Fish density(f) and biomass(f) estimations sometimes exhibit differences between frequencies, which is not fully in agreement with theoretical calculations. The aim of this study was to evaluate frequency comparisons in practise. However, if the differences on acoustic metrics, density(f) or biomass(f) between frequencies were occasionally statistically significant, the differences were small enough to be considered negligible for fish population management. These analyses led to better knowledge of the responses from fish in temperate lakes for the studied frequencies. Our findings should be considered when revising the CEN standard.

Parasitic versus nutritional regulation of natural fish populations.

Although parasites are expected to affect their host's fitness, quantitative proof for impacts of parasitism on wild populations is hampered by confounding environmental factors, including dietary resource. Herein, we evaluate whether the physiological conditions of European perch (Perca fluviatilis) in three large peri‐alpine lakes (Geneva, Annecy, and Bourget) depend on (a) the nutritional status of the juvenile fish, as revealed by stable isotope and fatty acid compositions, (b) the prevalence of the tapeworm Triaenophorus nodulosus, a parasite transmitted to perch through copepod preys, or (c) interactive effects of both factors. At the scale of lake populations, the deficit in growth and fat storage of juvenile perch during their first summer coincides with a high parasite prevalence and also a low quality of dietary resource. Yet, at the individual level, parasites had no evident effect on the growth of the juvenile perch, while impacts on fat storage appeared only at the highest prevalence of the most infected lake. Fatty acid and stable isotope analyses of fish tissue do not reveal any impact of T. nodulosus on diet, physiology, and feeding behaviour of fish within lakes. Overall, we found a low impact of parasitism on the physiological condition and trophic status of juvenile perch at the end of their first summer. We find instead that juvenile perch growth and fat storage, both factors tied to their winter survival, are under strong nutritional constraints. However, the coinciding nutritional constraints and parasite prevalence of perch juveniles in these three lakes may result from the indirect effect of lake nutrient concentrations, which, as a major control of zooplankton communities, simultaneously regulate both the dietary quality of fish prey and the host–parasite encounter rates.

Assessing the diversity of the g23 gene of T4-like bacteriophages from Lake Baikal with high-throughput sequencing.

Based on second generation sequencing (MiSeq platform, Illumina), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obtained by the Sanger method before. We obtained 33 701 sequences of the g23 gene fragments, 141 operational taxonomic units (OTUs) of which were identified. 86 OTUs (60.9%) had the closest relatives from lakes Bourget and Annecy, and 28 OTUs (19.8%) had the highest identity with the Baikal g23 clones, which had been previously identified in the northern and southern basins of the lake by the Sanger method. The remaining OTUs were similar to the clones from other ecosystems. We showed a high genetic diversity of T4-type bacteriophages and a genetic difference with the phage communities from other ecosystems.

Local human pressures influence gene flow in a hybridizing Daphnia species complex.

Anthropogenic environmental changes are considered critical drivers of the genetic structure of populations and communities through, for example, the facilitation of introgressive hybridization between syntopic species. However, the mechanisms by which environmental perturbations trigger changes in the genetic structure of populations and communities, such as the processes that determine the directionality of hybridization and patterns of mitochondrial introgression over many generations, remain largely unexplored. In this study, the changes in genetic structure of hybridizing members of the Daphnia longispina species complex were reconstructed over the last 100 years for three large temperate lakes under strong anthropogenic pressures via palaeogenetic analyses of resting egg banks. Drastic changes in the genetic structure of the Daphnia community, associated with hybridization events between D. longispina and D. galeata and subsequent introgression, were detected in Lakes Geneva and Bourget. In Lake Bourget, these changes were induced by the successful establishment of D. galeata with rising phosphorus levels and reinforced by the sensitivity of D. longispina to fish predation pressure. In Lake Geneva, the pattern of hybridization during eutrophication is more likely a function of the original taxonomic composition of the species complex in this lake. Lakes seem to require at least a meso-oligotrophic status to allow D. galeata populations to establish and accordingly no D. galeata genotypes were found in the egg bank of oligotrophic Lake Annecy. In contrast to the generally assumed pattern of unidirectional hybridization in this species complex, bidirectional hybridization was recorded in Lakes Geneva and Bourget. Our results also demonstrate complex genetic trajectories within this species complex and highlight the irreversibility of changes in the genotypic architecture of populations driven by local human pressures. Finally, we show that extensive hybridization and introgression do not necessarily result in a large and homogenous hybrid swarm.

Structure and diversity of ssDNA Microviridae viruses in two peri-alpine lakes (Annecy and Bourget, France)

Microviridae is a subset of single-stranded DNA (ssDNA) viruses infecting bacteria. This group of phages has been previously observed to be very abundant (representing >90% of the total known viral metagenomic sequences) in Lake Bourget. However, this observation was made only during one period (in summer) and from a single sample collected at a single depth (near surface). This result suggests the importance of these viruses, poorly examined thus far, especially in fresh waters. In this study, performed on the two largest natural lakes in France (e.g. Lakes Annecy and Bourget), Microviridae structure was determined each month throughout the year (2011) using PCR-DGGE, with primers that target the major-capsid-protein-encoding gene VP1; cloning/sequencing was used to investigate their diversity. Our results confirm that Microviridae are diverse in peri-alpine lakes and are mainly represented by gokushoviruses. We also found for the first time ssDNA viruses belonging to Alpavirinae, another subfamily within Microviridae recently proposed by Krupovic and Forterre (2011), generally prophages infecting members of the Phylum Bacteroidetes. Our data also support highly variable community composition and dynamics of individual components whose patterns were different between lakes, suggesting distinct host communities and/or abiotic influences between the two ecosystems. We point out that most of the major observed ssDNA Microviridae viruses display boom-bust patterns (with a sharp increase/decline) in their dynamics, with high relative abundances, suggesting brutal control of hosts and rapid regulation of the host community structure.

High-resolution paleolimnology opens new management perspectives for lakes adaptation to climate warming

Varved lake sediments provide opportunities for high-resolution paleolimnological investigations that may extend monitoring surveys in order to target priority management actions under climate warming. This paper provides the synthesis of an international research program relying on >150 years-long, varved records for three managed perialpine lakes in Europe (Lakes Geneva, Annecy and Bourget). The dynamics of the dominant, local human pressures, as well as the ecological responses in the pelagic, benthic and littoral habitats were reconstructed using classical and newly developed paleo-proxies. Statistical modelling achieved the hierarchization of the drivers of their ecological trajectories. All three lakes underwent different levels of eutrophication in the first half of the XXth century, followed by re-oligotrophication. Climate warming came along with a 2°C increase in air temperature over the last century, to which lakes were unequally thermally vulnerable. Unsurprisingly, phosphorous concentration has been the dominant ecological driver over the last century. Yet, other human-influenced, local environmental drivers (fisheries management practices, river regulations) have also significantly inflected ecological trajectories. Climate change has been impacting all habitats at rates that, in some cases, exceeded those of local factors. The amplitude and ecological responses to similar climate change varied between lakes, but, at least for pelagic habitats, rather depended on the intensity of local human pressures than on the thermal effect of climate change. Deep habitats yet showed higher sensitivity to climate change but substantial influence of river flows. As a consequence, adapted local management strategies, fully integrating nutrient inputs, fisheries management and hydrological regulations, may enable mitigating the deleterious consequences of ongoing climate change on these ecosystems.

Changes in carbon sources fueling benthic secondary production over depth and time: coupling Chironomidae stable carbon isotopes to larval abundance.

Stable C isotope ratio (δ(13)C) values of chironomid remains (head capsules; HC) were used to infer changes in benthic C sources over the last 150 years for two French sub-Alpine lakes. The HCs were retrieved from a series of sediment cores from different depths. The HC δ(13)C values started to decrease with the onset of eutrophication. The HC δ(13)C temporal patterns varied among depths, which revealed spatial differences in the contribution of methanotrophic bacteria to the benthic secondary production. The estimates of the methane (CH4)-derived C contribution to chironomid biomass ranged from a few percent prior to the 1930s to up to 30 % in recent times. The chironomid fluxes increased concomitantly with changes in HC δ(13)C values before a drastic decrease due to the development of hypoxic conditions. The hypoxia reinforced the implication for CH4-derived C transfer to chironomid production. In Lake Annecy, the HC δ(13)C values were negatively correlated to total organic C (TOC) content in the sediment (Corg), whereas no relationship was found in Lake Bourget. In Lake Bourget, chironomid abundances reached their maximum with TOC contents between 1 and 1.5 % Corg, which could constitute a threshold for change in chironomid abundance and consequently for the integration of CH4-derived C into the lake food webs. Our results indicated that the CH4-derived C contribution to the benthic food webs occurred at different depths in these two large, deep lakes (deep waters and sublittoral zone), and that the trophic transfer of this C was promoted in sublittoral zones where O2 gradients were dynamic.

Historical profiles of PCB in dated sediment cores suggest recent lake contamination through the "halo effect".

We investigated the major sources of polychlorinated biphenyls (PCB) and interpreted the environmental fate processes of these persistent organic pollutants in the past and current PCB contamination of three large, urbanized, French peri-alpine lakes. Dated sediment cores were analyzed in order to reconstruct and compare the historical contamination in all three lakes. Stratigraphic changes of PCB contents and fluxes were considered as revealing the temporal dynamics of PCB deposition to the lakes and the distribution of the seven indicator congeners (further referred to as PCBi) as an indicator of the main contamination origin and pathway. Although located within a single PCB industrial production region, concentration profiles for the three lakes differed in timing, peak concentration magnitudes, and in the PCBi congeners compositions. PCBi fluxes to the sediment and the magnitude of the temporal changes were generally much lower in Lake Annecy (0.05-2 ng·cm(-2)·yr(-1)) as compared to Lakes Geneva (0.05-5 ng·cm(-2)·yr(-1)) and Bourget (5-290 ng·cm(-2)·yr(-1)). For all three lakes, the paramount contamination occurred in the early 1970s. In Lakes Annecy and Bourget, PCB fluxes have declined and plateaued at 0.5 and 8 ng·cm(-2)·yr(-1), respectively, since the early 1990s. In Lake Geneva, PCB fluxes have further decreased by the end of the XX(th) century and are now very low. For the most contaminated lake (Lake Bourget), the high PCBi flux (5-290 ng·cm(-2)·yr(-1)) and the predominance of heavy congeners for most of the time period are consistent with a huge local input to the lake. This still high rate of Lake Bourget is explained by transport of suspended solids from one of its affluents, polluted by an industrial point source. Intermediate historical levels and PCBi distribution over time for Lake Geneva suggest a mixed contamination (urban point sources and distant atmospheric transport), while atmospheric deposition to Lake Annecy explains its lowest contamination rate. The presently low contamination levels recorded in Lake Geneva correspond to atmospheric inputs, but the recent PCBi distribution of Lake Annecy, enriched in relatively heavy congeners, reveals a contamination by the neighboring Lake Bourget, following a halo effect of about 40 km radius.

Blue-Green Algae in a “Greenhouse Century”? New Insights from Field Data on Climate Change Impacts on Cyanobacteria Abundance

Climate warming is likely to impact phytoplankton communities by providing a habitat in which cyanobacteria have competitive advantage over other phytoplankton taxa. We used extreme hot weather periods to investigate the potential impact of climate change on cyanobacteria abundance in three large and deep peri-alpine lakes, Lakes Geneva, Annecy, and Bourget. Between 2000 and 2011, there were four extreme warm weather periods: spring and summer 2003, autumn 2006 and winter 2007. We found that the consequences of extreme air temperatures on cyanobacteria abundance and phytoplankton composition depend on the time of year in which the extreme temperatures occur. In all three lakes studied, a warm summer did not clearly promote cyanobacteria blooms, whereas a warm autumn promoted cyanobacteria growth in the mesotrophic Lakes Geneva and Bourget, but not in the oligotrophic Lake Annecy. A warm winter was associated with high cyanobacteria abundance and a high contribution of cyanobacteria to total phytoplankton biomass. Our results reinforce the idea that lakes have an ecological memory by showing that a warm winter can influence subsequent seasonal succession in the cyanobacteria community. In both mesotrophic lakes studied, cyanobacteria abundance was strongly influenced by phosphorus concentrations and winter air temperatures. We conclude that although extreme hot weather periods can be used to analyze various aspects of the impacts of climate change, they are of limited value in forecasting the structure of phytoplankton communities in a warmer future.

Life history traits of the fish community in Lake Annecy: evidence from the stable isotope composition of otoliths

Stable isotope analysis (SIA) of fish otoliths shows great potential for exploring the ecology of fish, but this method has not been applied to the study of lacustrine fish communities. Both sequential and whole-otolith SIAs were performed on six fish species of Lake Annecy and their results compared to muscle SIA. The first purpose of these investigations was to test the use of δ18Ooto values for reconstructing fish thermal history and delimiting spatial distribution in a stratified lake. Comparison of species-specific fractionation equations and the general equation developed for freshwater fishes showed that the general one was the best suited for thermal estimation of Lake Annecy fishes and suggested that inter-specific differences or specific “vital effects” are not the only reason for apparent difference in fractionation. Thermal estimations based on SIA were consistent with descriptions of thermal habitats in the literature, except in the case of roach (Rutilus rutilus). Based on the current results, roach appears to live in a colder habitat than do perch (Perca fluviatilis). The high water transparency and thermal stratification of Lake Annecy could explain this distribution. Moreover, perch juveniles were found to live in two different thermal niches. This finding highlights the great plasticity of the species. Second, the potential use of δ13Coto values to reconstruct variation in diet and metabolism was assessed. The proportion of metabolic carbon (M) contributing to otolith carbon varies markedly among species. Comparison with δ13Cmuscle values shows no direct relationship between δ13Coto and diet at either the intra-individual or the inter-specific level. A strong linear relationship between either M or the isotopic offset between otolith and muscle (Δδ13Coto - muscle) and δ18Ooto values was found; this relationship reveals the dependence of M on the ambient temperature at which the species occurs. This relationship might be general and could be used to explore variation in fish metabolism in the future.