Lagrangian trajectories obtained through 3D Particle Tracking Velocimetry (3D-PTV) measurements have been used to visualize the flow field of Newtonian and non-Newtonian fluids in a flat-bottomed vessel. The vessel, of diameter T=180 mm, was equipped with a 6-blade Rushton turbine of diameter D=T/3 and four baffles of width b=T/10. The experiments were carried out in the transitional flow regime (73≤Re≤1,257). The velocities and Lagrangian accelerations in the flows have been calculated from the time-resolved tracer coordinates. Non-Newtonian fluids exhibited a smaller impeller flow number compared to Newtonian fluids. The distributions of shear rate have been obtained via interpolation of the Lagrangian velocity data in a 3D Eulerian grid. In the impeller region, the mean shear rate was, to a first approximation, proportional to the impeller rotational speed, although a more detailed analysis revealed influences of both rheology and Reynolds number. The mean Lagrangian acceleration scaled with the mean shear rate raised to the power of 1.8.