Lagerstroemia Speciosa Research Articles

Abnormal expression of LiASY1 and LiDMC1 is the main cause of sterility in F1 hybrids developed from Lagerstroemia speciosa and Lagerstroemia indica ‘Ebony Embers’

Lagerstroemia × ‘Xiaoxiang Huaxiu’ (an F1 hybrid of Lagerstroemia speciosa and Lagerstroemia indica ‘Ebony Embers’) exhibits significant sterility. Cytological studies have revealed numerous abnormal chromosomal behaviors during the meiotic division of pollen mother cells in L. × ‘Xiaoxiang Huaxiu’. Transcriptome sequencing identified that LiDMC1 and LiASY1 were expressed at very low levels in ‘Xiaoxiang Huaxiu’. To further verify whether the abnormal expression of LiDMC1 and LiASY1 was the fundamental cause of sterility in ‘Xiaoxiang Huaxiu’, we cloned the full-length gene sequences of LiDMC1 and LiASY1 from ‘Ebony Embers’. Using VIGS (virus-induced gene silencing) technology, pTRV1 and pTRV2 gene silencing vectors were employed to silence LiDMC1 and LiASY1 in the paternal parent ‘Ebony Embers’. The results indicated that in the double-silenced lines of ‘Ebony Embers’, the cytoplasm sizes of different tetrads during the tetrad stage varied significantly. There was a notable proportion of polyads, binucleate cells with micronuclei, and pentads. Additionally, pollen diameter varied significantly, closely resembling the sterility observed in ‘Xiaoxiang Huaxiu’. Pollen viability in the double-silenced ‘Ebony Embers’ decreased by 22.8%. This study demonstrates that the low expression of LiDMC1 and LiASY1 leads to abnormal microspore development in ‘Ebony Embers’, and their low expression is the cause of sterility in ‘Xiaoxiang Huaxiu’.

Lagerstroemin from Lagerstroemia speciosa as Antibreast Cancer Candidate Targeting AURKA, EGFR and SRC Protein: A Comprehensive Computational Study

Breast cancer is a type of cancer that has a high rate of diagnosis and mortality in the world. Lagerestroemia speciosa is a herb that has anticancer activity. This study aims to analyze the compounds in L. speciosa that most act as anticancer of the breast. The compounds in L. speciosa were selected based on drug-likeness, physicochemical properties, ability to penetrate the lipid bilayer and toxicity. The Lagerstroemin target related to Lagestroemin breast cancer was predicted using DisGeNET, SWISS Target Prediction and cBioportal. Molecular docking between Lagerstroemin and AURKA, EGFR and SRC was performed using AutoDock Vina. The interaction stability of each complex was analyzed by molecular dynamic simulation using YASARA with parameters of RMSD protein, RMSD ligand, number of hydrogen bonds and molecular dynamic binding energy. Of the 22 compounds, Quercetin, Caffeic acid, Lagerstroemin and Dypirone were predicted to have good ADME properties and can easily penetrate lipid membranes. Therefore, Quercetin, Caffeic acid and Lagerstroemin were predicted to have anti-breast cancer bioactivity. Of the 3 compounds, Lagerstroemin had the lowest toxicity. Lagerstroemin was predicted to interact with breast cancer-related proteins AURKA, EGFR and SRC. Molecular docking and dynamics showed that Lagerestroemin interacted stably at the ATP binding site of the 3 proteins, so it was very potential as an inhibitor of these 3 proteins. Therefore, Lagerstroemin was predicted to be the compound in L. speciosa with the most potential breast anticancer agent by targeting AURKA, EGFR and SRC. HIGHLIGHTS Quercetin, Caffeic acid and Lagerstroemin from Lagerstroemia speciosa show promising anticancer properties for breast cancer treatment. Lagerstroemin stands out with low toxicity and potent anticancer activity, suggesting its safety and effectiveness as a therapeutic agent. Lagerstroemin interacts stably with breast cancer-related proteins AURKA, EGFR and SRC, indicating its potential as a powerful inhibitor targeting key pathways in breast cancer. GRAPHICAL ABSTRACT

Identification of Bioactive Substances Derived from the Probiotic-Induced Bioconversion of Lagerstroemia speciosa Pers. Leaf Extract That Have Beneficial Effects on Diabetes and Obesity.

Lagerstroemia speciosa L. (Banaba) has been used as a functional food because of its diuretic, decongestant, antipyretic, anti-hyperglycemic, and anti-adipogenic activities. Triterpene acids, including corosolic acid, oleanolic acid, and asiatic acid, are the principal phytochemicals in Banaba and are potentially anti-diabetic substances, owing to their effect on blood glucose concentration. Bioconversion of Banaba leaf extract (BLE) by Lactobacillus plantarum CBT-LP3 improved the glucose uptake, insulin secretion, and fat browning of this functional food. Furthermore, we identified asiatic acid, which was found to be increased by 3.8-fold during the L. plantarum CBT-LP3-mediated bioconversion process using metabolite profiling. Most previous studies have focused on corosolic acid, another triterpene acid that is a known anti-diabetic compound and is used to standardize BLE preparations. However, asiatic acid is the second most common of the triterpene acids and is also well known to have anti-diabetic properties. The present study has provided strong evidence that asiatic acid represents an alternative to corosolic acid as the most important active compound. These results suggest that the probiotic-mediated bioconversion of BLE may improve the anti-diabetic effects of this functional food. This implies that the consumption of a probiotic should be encouraged for people undergoing BLE treatment to improve its anti-diabetic effects.

The Efficacy and Safety of DLBS3233, A Combined Bioactive Fraction of Cinnamomum burmanii and Lagerstroemia speciosa Plants on The Endocrine-Metabolic Profile of Women with Polycystic Ovary Syndrome: A Randomized Clinical Trial.

A bioactive fraction of Cinnamomum burmanii and Lagerstroemia speciosa, DLBS3233, has recently been used for type-2-diabetes treatment due to its favorable effect on insulin sensitivity. The insulin resistance leading to metabolic syndrome is closely linked to hyperandrogenemia in polycystic ovary syndrome (PCOS). This study evaluated the metabolic and reproductive efficacy and safety of DLBS3233 in insulin-resistant PCOS women. This was a 2-arm, randomized, double-blind, controlled, noninferiority clinical study over a 6-month therapy with DLBS3233 100-mg daily in comparison to metformin-XR 750 mg twice daily, involving 124 PCOS women with insulin resistance. The primary efficacy endpoint was the improvement of Homeostasis Model Assessment-Insulin Resistance (HOMA-IR). Secondary endpoints were improvements in other metabolic and reproductive parameters. Safety endpoints were based on blood pressure, heart rate, electrocardiogram findings, liver and renal function, and adverse events. After 6 months, HOMA-IR improvement in DLBS3233-treated group (-1.03 ± 0.50) and metformin-XR (-1.19 ± 0.50) were comparable, with a between-group difference fell within the pre-set non-inferiority margin (0.16; 95% confidence interval (CI): -1.24, 1.56; P=0.3168). The HOMA-IR in both groups were significantly improved from baseline. On all secondary endpoints, both groups showed comparable effects. Markedly fewer adverse events occurred in the DLBS3233 treated group than in the Metformin-XR-treated group and most were mild clinically and had been resolved by the end of the study. Treatment with DLBS3233 100-mg daily in PCOS women demonstrated comparable efficacy to metformin- XR 750-mg twice daily in improving insulin resistance. However, the non-inferiority of DLBS3233 to metformin- XR remains inconclusive. DLBS3233 was more tolerable than metformin-XR (registration number: NCT01733459).

Liquid Smoke Yield Grade 3, Grade 2 and Grade 1 from Five Different Types of Plants

This research was motivated by the lack of optimal use of leaves in forest plants, while there are many types of leaves in the forest. One use of leaves is to use them as raw material for making liquid smoke. This research was conducted to determine the yield of liquid smoke on forest plant species from their leaves. The research method used is the pyrolysis method with indirect combustion. This research was carried out by treating forest plant raw materials, namely kerai paying leaves (Filicium decipiens L.), bungur leaves (Lagerstroemia speciosa), mahogany leaves (Swietenia macrophylla King), teak leaves (Tectona grandis) and sungkai leaves (Peronema canecens Jack). The results of the research showed that the results of liquid smoke from the 5 types of plant leaves, namely sungkai leaves, had the highest grade 3 yield of 23.83% and the lowest grade 3 yield, namely mahogany leaves, of 17.81%. In grade 2 sungkai leaves the highest percentage was 21.12% and mahogany leaves had the lowest yield with a percentage of 16.18%. Meanwhile, in grade 1, sungkai leaves have the highest yield percentage of 19.33% and mahogany leaves have the lowest yield, namely 15.03%.

Evaluation of valoneic acid and the cytostasis properties of lagerstroemia speciosa ethanolic leaf extract on the accumulation of MPS in the complete blood count of the albino rats

Evaluation of valoneic acid and the cytostasis properties of lagerstroemia speciosa ethanolic leaf extract on the accumulation of MPS in the complete blood count of the albino rats

Enhanced performance of Lagerstroemia speciosa seed biochar and polypyrrole composite for the sequestration of emerging contaminant from wastewater sample: Case study of ofloxacin drug

Pharmaceutical effluents containing fluroquinolones (FQs) are one of the incipient organic impurities in aquatic environment. The ecosystem and human health were seriously affected from the remaining FQs in marine environment because of their toxicity. Hence, elimination of this FQs by emerging adsorbents with higher efficacy needs to be developed. This current research intends to developed a novel chemically modified jarul biochar/polypyrrole (CLSHB/PPy) composite via pyrolysis followed by sodium hydroxide modification and oxidative polymerization of pyrrole monomer to sequestrate ofloxacin (OFX) from aqua matrix. The characterization results of fabricated composite revealed that agglomerated spherical shape particles and their crystallinity nature can be act as an adequate adsorbent. Several sorption progression limitations are optimized which impact the interaction between CLSHB/PPy and OFX species. The optimum OFX eradication (%) of 82.42 ± 0.90 % by CLSHB/PPy was detected at pH 7.0, dose 1.0 g/L, and initial concentration 60 mg/L. Furthermore, Langmuir isotherm exhibited well fitted (R2 = 0.99) investigational data with an elimination ability of 50.02 ± 0.65 mg/g. Besides, kinetic analysis exposed that Pseudo second order model contribute the best suited kinetic. Thermodynamic study revealed that sorption progression of OFX onto CLSHB/PPy was both spontaneous and endothermic (∆H° ˃ 0). Moreover, CCD optimization exposed that investigational outcome (84.81 ± 1.02 %) are also adjacent to model integrated outcomes (84.12 ± 0.85 %), which indicates importance of RSM analysis. Stability analysis of composite (CLSHB/PPy) has been executed and exposed that the composite material can be effectively reutilized for OFX sorption after certain cycles.

Inventorying and proposing solutions for street tree management in Thu Duc city, Ho Chi Minh City

Tree inventory plays an essential role in the urban landscape master plan. It serves as the foundation for data acquisition that supports the planning strategy and decisions relating to the community’s interests, particularly in recently established cities like Thu Duc city. This research was carried out from October 2021 to December 2022 in this city and aimed to inventory to gather qualified information for managing the city’s street trees. The study used an inventory form of street trees including criteria such as name, family, diameter at breast height (DBH), total height, and indicators of vitality and distance from trees to infrastructure. By this form, 287 streets were surveyed. The total number of investigated trees was 31.023 trees belonging to 65 species and 24 flora families, of which the species with a high percentage (from 10% or more) were Dipterocarpus alatus, Hopea odorata, Peltophorum pterocarpum, and Lagerstroemia speciosa. The most numerous plant family was Fabaceae. These trees typically had a height of less than 10 m, with a DBH of less than 20 cm. Many streets did not guarantee the distance between trees and infrastructure, particularly the distance to the electrical systems. The survey also showed that the majority of trees were healthy and grow healthily. From the current situation of Thu Duc city street trees, three solutions must be prioritized in order to improve street trees in this city.

Lagerstroemia speciosa (Banaba) seed as a new and prospective bioenergy resource

Bio-oil, a highly valuable product obtained from biomass pyrolysis after proper up-gradation and refining, can be utilised in a variety of downstream applications which is gaining attention to the researcher from last few decades. Extraction and fractionation are two viable techniques to upgrade bio-oil those divide the complex mixture of bio-oil compounds into distinct fine chemicals and fractions enriched classes of chemical compounds. In the current investigation, a new biomass plant (Lagerstroemia speciosa) seed, commonly known as Banaba underwent thermo-chemical conversion at four terminal temperatures viz., 350 0C, 450 0C, 550 0C and 650 0C with a rate of heating 10 0C/min. The highest bio-oil yield (59.36 % out of that 41.68 % is of oil phase) was attained at pyrolysis temperature of 550 °C. The optimum condensable liquid (bio-oil) was collected and analysed. Properties of Banaba were investigated by physico-chemical, biochemical, ultimate analysis, TGA/DTG and FTIR analysis. Characterization of condensable liquid (bio-oil) was investigated by FTIR, NMR and GC-MS. Fuel property of the condensable liquid was further analysed through flash point, pour point, viscosity, Calorific value, and ultimate analysis. The Hydrogen/Carbon molar ratio (1.49) of the bio-oil was determined to be on a level with petroleum-based diesel. The oil derived from Banaba is found to have considerable fuel properties and may be substituted for commercial fuel with chemical modification or through blending with conventional diesel for utilization in IC engine which need to be addressed further.

Evaluation of the invitro antidiabetic and anti-cholesterols activity of selected plant extract

Abstract Diabetic and hypercholesterolemia are the most common metabolic disorders in the world. The purpose of the study was to assess the anti-diabetic and anti-cholesterol effect of water leaf extract of Lagerstroemia speciosa, Cinnamomum parthenoxylon, and Oroxylum indicum in vitro. Those three plants are often used by the local community to treat diabetes and hypercholesterol. The water extract’s active ingredients are steroids and flavonoids. The flavonoid content was reported to be 51.89, 110.92, and 63.41 mg QE/g respectively. In vitro studies on hemoglobin glycosylation, showed that 30 ppm aqueous leaf extract of L. speciosa, C. parthenoxylon, and O. indicum showed considerable inhibition of hemoglobin glycosylation by 12.06 %, 33.25%, and 73.96%, compared to a positive control (metformin) was 30.6 %. The in vitro anti-cholesterol properties of O. indicum and C. parthenoxylon could lower cholesterol by 33% compared to simvastatin by 33.104%. According to the study’s findings, the water leaf extracts of O. indicum and C. parthenoxylon have excellent pharmaceutical potential and might be used as herbal remedy to manage diabetes and cholesterol. To verify this work, more in vitro and in vivo research is required.

Reliable callus-induced plantlet regeneration from leaf explants of Lagerstroemia speciosa and genetic fidelity assessment through ISSR markers

Reliable callus-induced plantlet regeneration from leaf explants of Lagerstroemia speciosa and genetic fidelity assessment through ISSR markers

Comparative transcriptome and metabolite profiling reveal diverse pattern of CYP-TS gene expression during corosolic acid biosynthesis in Lagerstroemia speciosa (L.) Pers.

Extensive leaf transcriptome profiling and differential gene expression analysis of field grown and elicited shoot cultures of L. speciosa suggest that differential synthesis of CRA is mediated primarily by CYP and TS genes, showing functional diversity. Lagerstroemia speciosa L. is a tree species with medicinal and horticultural attributes. The pentacyclic triterpene, Corosolic acid (CRA) obtained from this species is widely used for the management of diabetes mellitus in traditional medicine. The high mercantile value of the compound and limited availability of innate resources entail exploration of alternative sources for CRA production. Metabolic pathway engineering for enhanced bioproduction of plant secondary metabolites is an attractive proposition for which, candidate genes in the pathway need to be identified and characterized. Therefore, in the present investigation, we focused on the identification of cytochrome P450 (CYP450) and oxidosqualene cyclases (OSC) genes and their differential expression during biosynthesis of CRA. The pattern of differential expression of these genes in the shoot cultures of L. speciosa, elicited with different epigenetic modifiers (azacytidine (AzaC), sodium butyrate (NaBu) and anacardic acid (AA)), was studied in comparison with field grown plant. Further, in vitro cultures with varying (low to high) concentrations of CRA were systematically assessed for the expression of CYP-TS and associated genes involved in CRA biosynthesis by transcriptome sequencing. The sequenced samples were de novo assembled into 180,290 transcripts of which, 92,983 transcripts were further annotated by UniProt. The results are collectively given in co-occurrence heat maps to identify the differentially expressed genes. The combined transcript and metabolite profiles along with RT-qPCR analysis resulted in the identification of CYP-TS genes with high sequence variation. Further, instances of concordant/discordant relation between CRA biosynthesis and CYP-TS gene expression were observed, indicating functional diversity in genes.

Genomic evidence for rediploidization and adaptive evolution following the whole-genome triplication

Whole-genome duplication (WGD), or polyploidy, events are widespread and significant in the evolutionary history of angiosperms. However, empirical evidence for rediploidization, the major process where polyploids give rise to diploid descendants, is still lacking at the genomic level. Here we present chromosome-scale genomes of the mangrove tree Sonneratia alba and the related inland plant Lagerstroemia speciosa. Their common ancestor has experienced a whole-genome triplication (WGT) approximately 64 million years ago coinciding with a period of dramatic global climate change. Sonneratia, adapting mangrove habitats, experienced extensive chromosome rearrangements post-WGT. We observe the WGT retentions display sequence and expression divergence, suggesting potential neo- and sub-functionalization. Strong selection acting on three-copy retentions indicates adaptive value in response to new environments. To elucidate the role of ploidy changes in genome evolution, we improve a model of the polyploidization–rediploidization process based on genomic evidence, contributing to the understanding of adaptive evolution during climate change.

Formulation Development, Characterization, and Therapeutic Evaluation of Ethanolic Extract of L. speciosa Leaves in Breast Cancer

Background: Due to its possible antioxidant, anti-tumor, anti-diabetic, and anti-inflammatory qualities, Lagerstroemia speciosa (Banaba) (LS) is receiving more attention in med-ical therapy. LS is a natural chemical with few adverse effects and low toxicity, although it has poor water solubility and absorption. Aims and Objectives: This study aimed to create a nanoemulsion formulation utilising an ethanol-ic extract of LS leaves and test it for anticancer activities in the MCF-7 cell line. Methods: Lemongrass oil, Cremophor EL as the surfactant, and Transcutol HP as the co-surfactant were used to create the nanoemulsion. It was done to characterize the ethanolic extract of LS leaves optimised nanoemulsion (ELSN). The MTT test against the MCF-7 cell line was used to assess the anticancer efficacy of ELSN. Results: The average particle size of the optimized nanoemulsion formulation was 91.22 nm with a PDI value of 0.224, indicating a monodispersed system. Transmission electron microscopy has validated the spherical shape of droplet size. Nanoemulsion components, in association with herbal extract, contributed to a zeta potential value of -4.73 Mv. It was discovered that ELSN IC50 was 75.13 ± 3.29 μg/mL. Conclusion: This study confirms that the development of ELSN improves the poor water solu-bility and bioavailability of the drug as well as enhances the therapeutic efficacy.

Retracted: Lagerstroemia speciosa Ameliorated Blood Pressure in LNAME Induced Hypertension in Experimental Rats through NO/cGMP and Oxidative Stress Modulation.

[This retracts the article DOI: 10.1155/2022/5894416.].

Molecular Mechanism of DLBS3233 Bioactive Fraction in Type-2 Diabetes Mellitus: Network Pharmacology and Docking Study

Although several antidiabetic agents are currently available, they commonly have undesirable effects and are not fully effective in reducing blood glucose. Therefore, since a long time ago, some Indonesians have preferred herbal medicine to cure and prevent diseases such as Lagerstroemia speciosa (L.) Pers. and Cinnamomum burmanni. (Nees & T.Nees) Blume, plants from Indonesia, are believed to be able to treat type 2 diabetes mellitus (T2DM). Both herbs are present in DLBS3233 bioactive fraction, a standardized herbal extract that acts as an insulin sensitizer like thiazolidinediones (TZDs). However, the molecular mechanisms, the bioactive compounds, and the target proteins involved remain unclear. To understand more about the potential molecular mechanism of DLBS3233 in treating T2DM, this network pharmacology study is conducted for the first time. Quercetin, kaempferol, and ellagic acid were discovered to have antidiabetic effects in this study as selected compounds of DLBS3233, primarily on eight core target proteins, including AKT1, EGFR, GSK3B, IL6, PTK2, RELA, SRC, and VEGFA. We also found that they exhibited ligand-receptor binding activity comparable to pioglitazone in the molecular docking study. Concisely, as a reference for furthering the development of this bioactive fraction, this study provides novel information on DLBS3233 in T2DM treatment that was not shown in prior studies.

Phenolic content and antioxidant capacity of Lagerstroemia speciosa (Banaba) and Blumea balsamifera (Sambong) herbal teas sold on-line in the Philippines: A cost-effectiveness analysis

Herbal teas are widely consumed for their health benefits. In this study, the phenolic content and antioxidant capacity of six commercially available banaba (Lagerstroemia speciosa) and sambong (Blumea balsamifera) herbal teas were analyzed. The antioxidant activity was measured using DPPH and ABTS assays, with L. speciosa teas exhibiting higher activity than B. balsamifera teas. The phenolic content was determined using the Folin-Ciocalteu method and showed a direct correlation with antioxidant activity. The market prices based on unit phenolic content and antioxidant activity varied widely with different brands sold online indicating the need for better quality control of the teas analyzed. Bangladesh J. Sci. Ind. Res. 58(4), 265-270, 2023

Corosolic Acid Induced Apoptosis via Upregulation of Bax/Bcl-2 Ratio and Caspase-3 Activation in Cholangiocarcinoma Cells.

Cholangiocarcinoma (CCA), an aggressive malignancy arising from the biliary epithelium, exhibits a high incidence in Thailand. CCA usually lacks specific symptoms and is typically diagnosed in its advanced stages, presenting significant treatment challenges. Current CCA therapeutic options, including surgery, chemotherapy, and radiation, have limited success rates and often cause side effects. Nature-derived compounds hold promise for reducing undesirable adverse effects and are an excellent source of anticancer drugs. Corosolic acid (CA), a triterpenoid found in Lagerstroemia speciosa L. leaves, exhibits anticancer properties; however, the effectiveness of CA against CCA and its molecular mechanisms remained unexplored. Herein, the anti-CCA and apoptosis-inducing effects of CA were investigated using various techniques, i.e., the MTT assay, flow cytometry with FITC-labeled Annexin V (Annexin V-FITC) and propidium iodide double staining, JC-1 staining, western blot analysis, caspase-3 activity assay, and molecular dynamics (MD) simulations. CA inhibited the proliferation of KKU-213A and KKU-213B CCA cells and triggered apoptosis through alterations in mitochondrial membrane potential (ΔΨm), and increases in the Bax/Bcl-2 expression ratio, cytochrome c release, and caspase-3 activity. As indicated by MD simulations, CA has the potential to bind to Bcl-2 through hydrogen bonds between amino acid residues R146 and N143. These findings underscore the potential of CA as a promising candidate for treatment of CCA.

ANTICALCIURIA EFFECT OF METHANOLIC ROOT EXTRACT OF LAGERSTROEMIA SPECIOSA (L). PERS (LYTHRACEAE) AGAINST HIGH PROTEIN DIET INGESTED IN ALBINO RATS

Nephrolithiasis, commonly known as kidney stone disease or urolithiasis, is a crystallopathy in which a solid mass forms in the urinary tract. The aim of the present research is to evaluate the way that albino rats administered with a high-protein diet, react to methanolic root extracts of Lagerstroemia speciosa anticalciuric properties. Medicinal plants are frequently utilised in folk medicine to treat a wide range of illnesses. Banaba is the common name for L. speciosa, a member of the Lythraceae family; Poomaruthu is its Tamil name. The test animals were given a diet high in protein and low in protein as well as 45 days of treatment with methanolic root extracts of L. speciosa. The experiment was scheduled with a sample collection, biochemical inquiries, and histopathological exams. The experiment had been scheduled with a sample collection, biochemical investigations, and histopathological evaluations. The biochemical data reveal that as compared to the control, III, and V groups, the low-protein diet groups II and IV had greater blood urea concentrations. The low-protein diet groups II and IV exhibited an increased blood creatinine concentration in contrast to the control, III, and V groups, while group V (H.P+MRE 500 mg/kg) demonstrated a nearly identical outcome to that of the control groups. L. speciosa root extracts in methanol are given to groups to control creatinine concentration. The normal control group's histopathological analysis revealed tubules with a single epithelial lining along the outermost layer that were normal in size.

Banaba Restricts Brain Damage: Neuroprotective Role in Cerebral Ischemiareperfusion Injury

Background:: Glucose regulation and energy homeostasis mitigate energy crises milieu in reperfusion injury. We investigated Banaba for its outcomes on cerebral ischemia reperfusion (IR) injury using artery occlusion in rats. The pleiotropic activity of Banaba on various debilitating mechanisms inducing reperfusion injury was evaluated. Aim:: This study aimedto evaluate the pharmacological activity of Banaba (Lagerstroemia speciosa) extract on reperfusion injury and investigate the effect of Banaba on vascular permeability, oxidative stress and cellular damage in ischemia reperfusion injury in rats. Methods:: Transient ischemia and reperfusion through occlusion of the middle cerebral artery (MCAO) lead to Cerebral IR injury in Wistar rats; it was treated with oral administration of Banaba extract (100mg/kg and 200mg/kg). The injury outcomes were evaluated after 22 hours of reperfusion by determining cellular injury, its impact on musculoskeletal coordination, multiple free radical scavenging measures (SOD, GSH, LPO) and vascular permeability of the blood-brain barrier. Result:: Banaba treatment led to a marked improvement in neurological outcomes by enhanced coordination and reduced cerebral infarct in comparison to vehicle control ischemic group. Free radical scavenging activity (SOD and GSH) was significantly better, and lipid peroxidation was reduced by Banaba treatment; it also reduced the vascular permeability of the blood-brain barrier. We observed that a lower dose of Banaba (100 mg/kg) was more effective than the higher (200 mg/kg) in ischemic rats. The anti-inflammatory and anti-oxidant activity could drive the neuroprotective outcomes of Banaba in cerebral IR injury. The critical factor of the beneficial effect of Banaba in cerebral injury is the optimization of dose in this experimental setup of reperfusion injury using rats. Conclusion:: The recovery of injury could be attributed to Banaba’s multi-factorial effect targeting free radicals, inflammation, and necrosis during ischemiareperfusion injury.