Experimental ultraviolet was performed in rabbit eyes in two series. Using optic-enzymatic tests we investigated pyruvate and lactate levels of the anterior eye segment after biomicroscopic controls of the damaged corneal epithelium. Comparing the untreated control eyes we found again the lactate concentration gradient of the cornea and aqueous humor. Lactate levels of the stroma and aqueous humor of the anterior chamber were reduced significantly, while the lactate pyruvate ratio was increased in corneal epithelium and reduced in the aqueous. The direct influence of ultraviolet radiation on nucleoproteins was discussed.