IntroductionIsolated lung experiments allow for ex vivo characterization of lung function and prove to be a valuable model for various fields such as cancer, lung transplantation, and ischemia/reperfusion injury. Unlike other models that require damaging the lung, the isolated lung allows for collection of physiological changes including airway pressure, pulmonary artery and vein pressure, airway flow, and, in our model, ventilated gas concentrations. We describe the use of LabVIEW combined with the free statistical software R for highly flexible, effective, and automated data collection in an isolated lung system.MethodsPressure transducers connected to bioamplifiers allow for collection of dynamic pulmonary artery and vein pressures, while airway pressure and flow are collected from the ventilator with the former used to visualize positive end expiratory pressure. Signals from these devices are connected to an analog‐digital converter from National InstrumentsTM that collects data into LabVIEW software, which is then automatically collected and displayed at 200Hz. Use of a roller pump with analog input/output allows for fully automated control of the perfusion speed. Output from a gas mixer for use in ventilated gas studies is collected and used to generate gas composition data. Additional experimental data that do not have signal output capability can be recorded manually in LabVIEW.ResultsLabVIEW is a reliable alternative to other commercially available data acquisition systems. The creation of a virtual instrument requires significant programming knowledge but allows for completely customizable data collection in a modular isolated lung system. Data become available in an Excel‐compatible file which can then be imported and analysed in R as another affordable alternative to commercial programs.ConclusionWe describe the design and implementation of a LabVIEW virtual instrument as an adjustable alternative to conventional data acquisition systems.Support or Funding InformationThis work was supported, in part, by institutional funds, NIH grant (5R01 HL123227), and a Merit Review Award (I01 BX003482) from the U.S. Department of Veterans Affairs Biomedical Laboratory R&D Service.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.